Rroxscaffold_1G00033470

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0031077
Physical Location & Seq
Reverse (-)
48471372 .. 48472797
1426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00033470.1

Sequence Viewer

Length: 555 bp
ATGAGTTTGAGCAATGGAATGAGGACCTGTGCCAAGGTCTTGATGTGTTCAGAAGAACCATTATCAAAATCAGGTTACTTTGATGTCATATGTTGCTTATTCTGGTTGTTGCCACTGTATTTCGATGGCAAGTATTTGCATAATATGACAATTGTAGAATCCATAGGATGTAATCAATTATCTAGAAATGCAGGTCACCTATCTAGTGGAAGGGGTACTTTTGTTGATAGAGCTGTCATTGCAGCAGCAAGAGCATTGCCTACTTGGTCAAATGGGGGATGTGCCTCCAATCTAAGCATGGAAAGAAGAACGATAAAGGAATTACAGGAAGAATGCAGACAGATGCTTGCCGCGTTTCCTACCAACTCTAAGGAAGACCAAAATATCCTAGTTTATGATCTGTCCACATTCACCATATCATTGCTTTCTGTTAAATATAGGGACTGGAAAAATGTCATGACAAAGTTATTTACTCGTGTATTTGCTTCACTTCCAGATGATCCAAAACTTGATGTTGTTGGTTATGTTTTCTTCTTTGCCTGGGCTCTATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

184

Amino Acids

20.74

Weight (kDa)

8.1

Isoelectric Point (pI)

34.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031077)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000516.1_g000001
rosa_roxburghii Rroxscaffold_1G00033470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 182
AccII CGCG 1 cut(s) 353
AciI CCGC 1 cut(s) 351
AclWI GGATC 1 cut(s) 494
AfaI GTAC 1 cut(s) 217
AjnI CCWGG 1 cut(s) 539
AluBI AGCT 1 cut(s) 233
AluI AGCT 1 cut(s) 233
AlwI GGATC 1 cut(s) 494
ApeKI GCWGC 2 cut(s) 242, 245
AspS9I GGNCC 1 cut(s) 24
AsuHPI GGTGA 2 cut(s) 188, 403
AvaII GGWCC 1 cut(s) 24
BaeI ACNNNNGTAYC 2 cut(s) 207, 240
BanII GRGCYC 1 cut(s) 547
BauI CACGAG 1 cut(s) 474
BbsI GAAGAC 1 cut(s) 381
BbvI GCAGC 2 cut(s) 254, 257
BccI CCATC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 541
BfaI CTAG 3 cut(s) 183, 204, 389
BfuAI ACCTGC 1 cut(s) 182
BisI GCNGC 3 cut(s) 243, 246, 351
BlsI GCNGC 3 cut(s) 244, 247, 352
Bme1390I CCNGG 1 cut(s) 541
Bme18I GGWCC 1 cut(s) 24
BmgT120I GGNCC 1 cut(s) 24
BmrFI CCNGG 1 cut(s) 541
BmsI GCATC 1 cut(s) 333
BpiI GAAGAC 1 cut(s) 381
BsaJI CCNNGG 2 cut(s) 33, 540
Bse1I ACTGG 1 cut(s) 449
Bse3DI GCAATG 4 cut(s) 19, 237, 254, 419
BseBI CCWGG 1 cut(s) 541
BseDI CCNNGG 2 cut(s) 33, 540
BseGI GGATG 2 cut(s) 173, 284
BseMI GCAATG 4 cut(s) 19, 237, 254, 419
BseNI ACTGG 1 cut(s) 449
BseXI GCAGC 2 cut(s) 254, 257
Bsh1236I CGCG 1 cut(s) 353
BslFI GGGAC 1 cut(s) 455
BsmFI GGGAC 1 cut(s) 455
BsmI GAATGC 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 547
Bsp143I GATC 2 cut(s) 397, 499
BspACI CCGC 1 cut(s) 351
BspFNI CGCG 1 cut(s) 353
BspHI TCATGA 1 cut(s) 456
BspMI ACCTGC 1 cut(s) 182
BspPI GGATC 1 cut(s) 494
BsrDI GCAATG 4 cut(s) 19, 237, 254, 419
BsrI ACTGG 1 cut(s) 449
BssECI CCNNGG 2 cut(s) 33, 540
BssMI GATC 2 cut(s) 397, 499
BssSI CACGAG 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 33
Bst2BI CACGAG 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 541
Bst4CI ACNGT 1 cut(s) 117
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 2 cut(s) 293, 369
BstEII GGTNACC 1 cut(s) 194
BstF5I GGATG 2 cut(s) 173, 284
BstFNI CGCG 1 cut(s) 353
BstKTI GATC 2 cut(s) 400, 502
BstMBI GATC 2 cut(s) 397, 499
BstMWI GCNNNNNNNGC 2 cut(s) 239, 251
BstNI CCWGG 1 cut(s) 541
BstPI GGTNACC 1 cut(s) 194
BstSCI CCNGG 1 cut(s) 539
BstUI CGCG 1 cut(s) 353
BstV1I GCAGC 2 cut(s) 254, 257
BstV2I GAAGAC 1 cut(s) 381
BtsCI GGATG 2 cut(s) 173, 284
BtsIMutI CAGTG 1 cut(s) 113
BveI ACCTGC 1 cut(s) 182
Cac8I GCNNGC 1 cut(s) 348
CciI TCATGA 1 cut(s) 456
Cfr13I GGNCC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 216
CviAII CATG 2 cut(s) 298, 457
CviJI RGCY 2 cut(s) 233, 545
CviKI_1 RGCY 2 cut(s) 233, 545
CviQI GTAC 1 cut(s) 216
DdeI CTNAG 2 cut(s) 293, 369
DpnI GATC 2 cut(s) 399, 501
DpnII GATC 2 cut(s) 397, 499
Eco130I CCWWGG 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 547
Eco47I GGWCC 1 cut(s) 24
Eco91I GGTNACC 1 cut(s) 194
EcoO109I RGGNCCY 1 cut(s) 24
EcoO65I GGTNACC 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 539
EcoT14I CCWWGG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 547
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 2 cut(s) 301, 460
FalI AAGNNNNNCTT 2 cut(s) 202, 234
FaqI GGGAC 1 cut(s) 455
FatI CATG 2 cut(s) 297, 456
FauNDI CATATG 1 cut(s) 89
Fnu4HI GCNGC 3 cut(s) 243, 246, 351
FokI GGATG 2 cut(s) 180, 291
FriOI GRGCYC 1 cut(s) 547
Fsp4HI GCNGC 3 cut(s) 243, 246, 351
FspBI CTAG 3 cut(s) 183, 204, 389
GluI GCNGC 3 cut(s) 243, 246, 351
Hin1II CATG 2 cut(s) 301, 460
HinfI GANTC 1 cut(s) 158
HphI GGTGA 2 cut(s) 188, 403
Hpy166II GTNNAC 1 cut(s) 405
Hpy188I TCNGA 1 cut(s) 52
Hpy188III TCNNGA 4 cut(s) 40, 183, 457, 494
Hpy8I GTNNAC 1 cut(s) 405
HpyAV CCTTC 1 cut(s) 204
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4V TGCA 4 cut(s) 139, 191, 242, 336
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 251
HpyF3I CTNAG 2 cut(s) 293, 369
Hsp92II CATG 2 cut(s) 301, 460
Kzo9I GATC 2 cut(s) 397, 499
LpnPI CCDG 8 cut(s) 40, 57, 88, 177, 311, 430, 507, 526
Lsp1109I GCAGC 2 cut(s) 254, 257
LweI GCATC 1 cut(s) 333
MaeI CTAG 3 cut(s) 183, 204, 389
MaeIII GTNAC 2 cut(s) 74, 194
MalI GATC 2 cut(s) 399, 501
MboI GATC 2 cut(s) 397, 499
MboII GAAGA 5 cut(s) 65, 318, 341, 386, 523
MfeI CAATTG 1 cut(s) 150
MhlI GDGCHC 1 cut(s) 547
MluCI AATT 3 cut(s) 150, 176, 320
MnlI CCTC 2 cut(s) 15, 295
MseI TTAA 2 cut(s) 432, 553
MspR9I CCNGG 1 cut(s) 541
MunI CAATTG 1 cut(s) 150
Mva1269I GAATGC 1 cut(s) 338
MvaI CCWGG 1 cut(s) 541
MvnI CGCG 1 cut(s) 353
MwoI GCNNNNNNNGC 2 cut(s) 239, 251
NdeI CATATG 1 cut(s) 89
NdeII GATC 2 cut(s) 397, 499
NlaIII CATG 2 cut(s) 301, 460
NmuCI GTSAC 1 cut(s) 194
PagI TCATGA 1 cut(s) 456
PctI GAATGC 1 cut(s) 338
PfeI GAWTC 1 cut(s) 158
PkrI GCNGC 3 cut(s) 244, 247, 352
PpuMI RGGWCCY 1 cut(s) 24
Psp5II RGGWCCY 1 cut(s) 24
Psp6I CCWGG 1 cut(s) 539
PspEI GGTNACC 1 cut(s) 194
PspGI CCWGG 1 cut(s) 539
PspPI GGNCC 1 cut(s) 24
PspPPI RGGWCCY 1 cut(s) 24
RsaI GTAC 1 cut(s) 217
RsaNI GTAC 1 cut(s) 216
SaqAI TTAA 2 cut(s) 432, 553
SatI GCNGC 3 cut(s) 243, 246, 351
Sau3AI GATC 2 cut(s) 397, 499
Sau96I GGNCC 1 cut(s) 24
ScrFI CCNGG 1 cut(s) 541
SduI GDGCHC 1 cut(s) 547
SetI ASST 6 cut(s) 29, 39, 76, 196, 201, 235
SfaNI GCATC 1 cut(s) 333
SinI GGWCC 1 cut(s) 24
Sse9I AATT 3 cut(s) 150, 176, 320
SsiI CCGC 1 cut(s) 351
SspMI CTAG 3 cut(s) 183, 204, 389
StyD4I CCNGG 1 cut(s) 539
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 1 cut(s) 117
TaqI TCGA 1 cut(s) 123
TasI AATT 3 cut(s) 150, 176, 320
TauI GCSGC 1 cut(s) 353
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 2 cut(s) 432, 553
Tru9I TTAA 2 cut(s) 432, 553
TscAI CASTG 1 cut(s) 120
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 2 cut(s) 242, 245
Tsp45I GTSAC 1 cut(s) 194
TspRI CASTG 1 cut(s) 120
VpaK11BI GGWCC 1 cut(s) 24
XbaI TCTAGA 1 cut(s) 182
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XspI CTAG 3 cut(s) 183, 204, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.