Rroxscaffold_2G00114450

Glucose-6-phosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0031366
Physical Location & Seq
Reverse (-)
40512421 .. 40512783
363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00114450.1

Sequence Viewer

Length: 363 bp
ATGGCTTCATCTGCTTTGATCTCTGATACTGAGCCTTGGAAGGATTTGAAGGGCCATGTTGAGGAGATAAACAAGACCCATTTGCGTGATTTGATGAATGATGTTAACCGGTGCCAGGCAATGATGGTTGAGTTTGATGGGATGCTGTTGGACTACTCGCGGCAACGTGCAACCCTTGAAACTATGGGGAAACTGATCAAATTGGCAGAGGCTGCAGGCCTCAAAGATAAGATCAACCGAATGTTTGCTGGAGAGCATATAAACAGCACAGAAAATAGATCAGTGCTTCATGTAGCTCTTCGTGCTCCAAGGGATGCAGTTATACAGAGTGATGGGAAAGAGTTGCAACTATTACATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004347 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005996 GO:0006006 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016853 GO:0016860 GO:0016861 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00500 ko00520 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00030 map00500 map00520 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
PGI

Protein Analysis

120

Amino Acids

13.56

Weight (kDa)

6.19

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGI PF00342 51 - 114 2.9e-19 Phosphoglucose isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031366)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0006254.1_g000001
rosa_roxburghii Rroxscaffold_2G00114450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 111
AccII CGCG 1 cut(s) 160
AciI CCGC 1 cut(s) 160
AfiI CCNNNNNNNGG 2 cut(s) 61, 115
AgeI ACCGGT 1 cut(s) 108
AgsI TTSAA 2 cut(s) 49, 179
AjnI CCWGG 1 cut(s) 114
AluBI AGCT 1 cut(s) 296
AluI AGCT 1 cut(s) 296
Alw21I GWGCWC 1 cut(s) 307
AlwNI CAGNNNCTG 1 cut(s) 212
AoxI GGCC 2 cut(s) 52, 217
ApeKI GCWGC 1 cut(s) 212
AsiGI ACCGGT 1 cut(s) 108
AspS9I GGNCC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 199
BccI CCATC 3 cut(s) 118, 131, 326
BciT130I CCWGG 1 cut(s) 116
BclI TGATCA 1 cut(s) 195
BfmI CTRYAG 1 cut(s) 213
BisI GCNGC 2 cut(s) 161, 213
BlsI GCNGC 2 cut(s) 162, 214
Bme1390I CCNGG 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 1 cut(s) 113
BmrFI CCNGG 1 cut(s) 116
BmsI GCATC 2 cut(s) 132, 304
BpmI CTGGAG 1 cut(s) 270
BsaJI CCNNGG 2 cut(s) 35, 308
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 115
Bse118I RCCGGY 1 cut(s) 108
Bse3DI GCAATG 1 cut(s) 126
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 2 cut(s) 35, 308
BseGI GGATG 2 cut(s) 147, 319
BseLI CCNNNNNNNGG 2 cut(s) 61, 115
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 1 cut(s) 21
BseRI GAGGAG 1 cut(s) 77
BseXI GCAGC 1 cut(s) 199
Bsh1236I CGCG 1 cut(s) 160
BshFI GGCC 2 cut(s) 54, 219
BshNI GGYRCC 1 cut(s) 111
BshTI ACCGGT 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 1 cut(s) 109
BslI CCNNNNNNNGG 2 cut(s) 61, 115
BsnI GGCC 2 cut(s) 54, 219
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 4 cut(s) 18, 195, 231, 278
BspACI CCGC 1 cut(s) 160
BspANI GGCC 2 cut(s) 54, 219
BspCNI CTCAG 1 cut(s) 22
BspFNI CGCG 1 cut(s) 160
BspLI GGNNCC 1 cut(s) 113
BspMAI CTGCAG 1 cut(s) 217
BspQI GCTCTTC 1 cut(s) 303
BspT107I GGYRCC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 126
BsrFI RCCGGY 1 cut(s) 108
BssAI RCCGGY 1 cut(s) 108
BssECI CCNNGG 2 cut(s) 35, 308
BssMI GATC 4 cut(s) 18, 195, 231, 278
BssT1I CCWWGG 2 cut(s) 35, 308
Bst2UI CCWGG 1 cut(s) 116
Bst6I CTCTTC 1 cut(s) 303
BstAPI GCANNNNNTGC 1 cut(s) 212
BstC8I GCNNGC 1 cut(s) 217
BstDEI CTNAG 1 cut(s) 30
BstF5I GGATG 2 cut(s) 147, 319
BstFNI CGCG 1 cut(s) 160
BstKTI GATC 4 cut(s) 21, 198, 234, 281
BstMBI GATC 4 cut(s) 18, 195, 231, 278
BstMWI GCNNNNNNNGC 3 cut(s) 11, 212, 302
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 114
BstSFI CTRYAG 1 cut(s) 213
BstUI CGCG 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 199
BsuRI GGCC 2 cut(s) 54, 219
BtsCI GGATG 2 cut(s) 147, 319
BtsIMutI CAGTG 1 cut(s) 288
Cac8I GCNNGC 1 cut(s) 217
CaiI CAGNNNCTG 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 108
Cfr13I GGNCC 1 cut(s) 52
CspAI ACCGGT 1 cut(s) 108
CviAII CATG 2 cut(s) 56, 290
CviJI RGCY 6 cut(s) 5, 34, 54, 212, 219, 296
CviKI_1 RGCY 6 cut(s) 5, 34, 54, 212, 219, 296
DdeI CTNAG 1 cut(s) 30
DpnI GATC 4 cut(s) 20, 197, 233, 280
DpnII GATC 4 cut(s) 18, 195, 231, 278
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Eco130I CCWWGG 2 cut(s) 35, 308
Eco147I AGGCCT 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 114
EcoT14I CCWWGG 2 cut(s) 35, 308
ErhI CCWWGG 2 cut(s) 35, 308
FaeI CATG 2 cut(s) 59, 293
FaiI YATR 6 cut(s) 57, 185, 258, 260, 291, 323
FatI CATG 2 cut(s) 55, 289
FbaI TGATCA 1 cut(s) 195
Fnu4HI GCNGC 2 cut(s) 161, 213
FokI GGATG 2 cut(s) 154, 326
Fsp4HI GCNGC 2 cut(s) 161, 213
GluI GCNGC 2 cut(s) 161, 213
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 2 cut(s) 54, 219
HapII CCGG 1 cut(s) 109
Hin1II CATG 2 cut(s) 59, 293
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HpaI GTTAAC 1 cut(s) 106
HpaII CCGG 1 cut(s) 109
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 1 cut(s) 25
Hpy8I GTNNAC 1 cut(s) 106
HpyAV CCTTC 2 cut(s) 34, 43
HpyCH4IV ACGT 1 cut(s) 166
HpyCH4V TGCA 4 cut(s) 170, 215, 317, 346
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 212, 302
HpyF3I CTNAG 1 cut(s) 30
HpySE526I ACGT 1 cut(s) 166
Hsp92II CATG 2 cut(s) 59, 293
Ksp22I TGATCA 1 cut(s) 195
KspAI GTTAAC 1 cut(s) 106
Kzo9I GATC 4 cut(s) 18, 195, 231, 278
LguI GCTCTTC 1 cut(s) 303
LmnI GCTCC 1 cut(s) 310
LpnPI CCDG 5 cut(s) 101, 122, 128, 201, 234
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 2 cut(s) 132, 304
MaeII ACGT 1 cut(s) 166
MalI GATC 4 cut(s) 20, 197, 233, 280
MboI GATC 4 cut(s) 18, 195, 231, 278
MboII GAAGA 1 cut(s) 290
MhlI GDGCHC 1 cut(s) 307
MluCI AATT 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 129
MnlI CCTC 3 cut(s) 55, 202, 230
MseI TTAA 1 cut(s) 105
MslI CAYNNNNRTG 1 cut(s) 84
MspI CCGG 1 cut(s) 109
MspR9I CCNGG 1 cut(s) 116
MvaI CCWGG 1 cut(s) 116
MvnI CGCG 1 cut(s) 160
MwoI GCNNNNNNNGC 3 cut(s) 11, 212, 302
NdeII GATC 4 cut(s) 18, 195, 231, 278
NlaIII CATG 2 cut(s) 59, 293
NlaIV GGNNCC 1 cut(s) 113
PceI AGGCCT 1 cut(s) 219
PciSI GCTCTTC 1 cut(s) 303
PinAI ACCGGT 1 cut(s) 108
PkrI GCNGC 2 cut(s) 162, 214
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 113
PspPI GGNCC 1 cut(s) 52
PstI CTGCAG 1 cut(s) 217
PstNI CAGNNNCTG 1 cut(s) 212
RseI CAYNNNNRTG 1 cut(s) 84
SapI GCTCTTC 1 cut(s) 303
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 2 cut(s) 161, 213
Sau3AI GATC 4 cut(s) 18, 195, 231, 278
Sau96I GGNCC 1 cut(s) 52
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 1 cut(s) 307
SetI ASST 2 cut(s) 169, 298
SfaNI GCATC 2 cut(s) 132, 304
SfcI CTRYAG 1 cut(s) 213
SmiMI CAYNNNNRTG 1 cut(s) 84
Sse9I AATT 1 cut(s) 200
SseBI AGGCCT 1 cut(s) 219
SsiI CCGC 1 cut(s) 160
StuI AGGCCT 1 cut(s) 219
StyD4I CCNGG 1 cut(s) 114
StyI CCWWGG 2 cut(s) 35, 308
TaiI ACGT 1 cut(s) 169
TasI AATT 1 cut(s) 200
TauI GCSGC 1 cut(s) 163
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 1 cut(s) 288
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 2 cut(s) 110, 278
TspRI CASTG 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.