Rroxscaffold_4G00285250

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0031154
Physical Location & Seq
Forward (+)
6626750 .. 6627301
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00285250.1

Sequence Viewer

Length: 552 bp
ATGAAACTTCGCAGACGTGTACTGGGATCCTGTAATGGCTTGATCTGTGTAGCTCAGGAAAACCTTTTTCTCTGGACCTTGGTTACTATCTGGAACCCAGTAACTGGATTTATGCATAACTTACCTGCTCCATATTTTTACCTAGACAGCTTCTTTATTACTGGCTTTGGCTATTTGTCGGCGACTGATGATTACAAAGTTCTTTTAGGCGGCTGTTCTGATACTACTAAGGTTCAGGTTTTCTCATTGAGAGCTAACATTTGGAAAACGATTGAAGGCCCTATCCTCCATCGTTTCAGGCCTCCCGGGGTTCTTTTAAATGTGACACTTCATTTTAAGCATACTAAAACACGTCTCCTGGCTTTCGATTTGGCGAAAGAAGAGTTCCGGGCAATGTGGCTTCCTGATTTTGAAGGAACAGGGCGGCGTGGGAGAGACAAAGACAAGGAGAATAGTTCGGTTTATGATCTTGGTGTTTGTGGAGGATGCCTGTATCTGGTTCATTGGGACCCTACACATAAACCTATTGGGTTGTGGCCTTGTGGGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

183

Amino Acids

20.84

Weight (kDa)

8.93

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 7 - 136 4.1e-11 F-box associated beta propeller domain
FBA_1 PF07734 10 - 137 4.2e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031154)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001755.1_g000026
rosa_roxburghii Rroxscaffold_4G00285250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 133
AccB7I CCANNNNNTGG 1 cut(s) 104
AciI CCGC 2 cut(s) 210, 424
AclWI GGATC 2 cut(s) 21, 34
AfaI GTAC 1 cut(s) 21
AfiI CCNNNNNNNGG 2 cut(s) 104, 496
AflIII ACRYGT 2 cut(s) 16, 350
AgsI TTSAA 2 cut(s) 275, 413
AjiI CACGTC 2 cut(s) 17, 353
AjnI CCWGG 1 cut(s) 357
AluBI AGCT 3 cut(s) 53, 150, 254
AluI AGCT 3 cut(s) 53, 150, 254
Alw26I GTCTC 2 cut(s) 359, 429
AlwI GGATC 2 cut(s) 21, 34
AlwNI CAGNNNCTG 1 cut(s) 104
Ama87I CYCGRG 1 cut(s) 305
AoxI GGCC 3 cut(s) 277, 299, 536
AspS9I GGNCC 3 cut(s) 75, 278, 508
AsuC2I CCSGG 3 cut(s) 306, 307, 389
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 2 cut(s) 75, 508
BamHI GGATCC 1 cut(s) 26
BccI CCATC 1 cut(s) 297
BciT130I CCWGG 1 cut(s) 359
BcnI CCSGG 3 cut(s) 306, 307, 389
BcoDI GTCTC 2 cut(s) 359, 429
BfaI CTAG 1 cut(s) 143
BfuAI ACCTGC 1 cut(s) 133
BisI GCNGC 2 cut(s) 211, 425
BlsI GCNGC 2 cut(s) 212, 426
Bme1390I CCNGG 4 cut(s) 306, 307, 359, 389
Bme18I GGWCC 2 cut(s) 75, 508
BmeT110I CYCGRG 1 cut(s) 305
BmgBI CACGTC 2 cut(s) 17, 353
BmgT120I GGNCC 3 cut(s) 75, 278, 508
BmiI GGNNCC 4 cut(s) 28, 95, 509, 510
BmrFI CCNGG 4 cut(s) 306, 307, 359, 389
BmrI ACTGGG 2 cut(s) 32, 92
BmsI GCATC 1 cut(s) 476
BmuI ACTGGG 2 cut(s) 32, 92
Bpu10I CCTNAGC 1 cut(s) 54
BpuMI CCSGG 3 cut(s) 306, 307, 389
BsaJI CCNNGG 3 cut(s) 78, 305, 306
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 496
Bse1I ACTGG 4 cut(s) 27, 98, 109, 166
Bse3DI GCAATG 1 cut(s) 399
BseBI CCWGG 1 cut(s) 359
BseDI CCNNGG 3 cut(s) 78, 305, 306
BseGI GGATG 1 cut(s) 491
BseLI CCNNNNNNNGG 2 cut(s) 104, 496
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 1 cut(s) 68
BseNI ACTGG 4 cut(s) 27, 98, 109, 166
BshFI GGCC 3 cut(s) 279, 301, 538
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 2 cut(s) 306, 388
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 2 cut(s) 104, 496
BsmAI GTCTC 2 cut(s) 359, 429
BsmBI CGTCTC 1 cut(s) 359
BsmFI GGGAC 1 cut(s) 521
BsnI GGCC 3 cut(s) 279, 301, 538
BsoBI CYCGRG 1 cut(s) 305
Bsp143I GATC 3 cut(s) 26, 42, 466
BspACI CCGC 2 cut(s) 210, 424
BspANI GGCC 3 cut(s) 279, 301, 538
BspCNI CTCAG 1 cut(s) 67
BspHI TCATGA 1 cut(s) 548
BspLI GGNNCC 4 cut(s) 28, 95, 509, 510
BspMI ACCTGC 1 cut(s) 133
BspPI GGATC 2 cut(s) 21, 34
BsrDI GCAATG 1 cut(s) 399
BsrI ACTGG 4 cut(s) 27, 98, 109, 166
BssECI CCNNGG 3 cut(s) 78, 305, 306
BssMI GATC 3 cut(s) 26, 42, 466
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 359
Bst6I CTCTTC 1 cut(s) 375
BstDEI CTNAG 2 cut(s) 54, 228
BstF5I GGATG 1 cut(s) 491
BstKTI GATC 3 cut(s) 29, 45, 469
BstMAI GTCTC 2 cut(s) 359, 429
BstMBI GATC 3 cut(s) 26, 42, 466
BstNI CCWGG 1 cut(s) 359
BstSCI CCNGG 4 cut(s) 304, 305, 357, 387
BstX2I RGATCY 1 cut(s) 26
BstYI RGATCY 1 cut(s) 26
BsuRI GGCC 3 cut(s) 279, 301, 538
BtrI CACGTC 2 cut(s) 17, 353
BtsCI GGATG 1 cut(s) 491
BveI ACCTGC 1 cut(s) 133
CaiI CAGNNNCTG 1 cut(s) 104
CciI TCATGA 1 cut(s) 548
Cfr13I GGNCC 3 cut(s) 75, 278, 508
Cfr9I CCCGGG 1 cut(s) 305
Csp6I GTAC 1 cut(s) 20
CviAII CATG 1 cut(s) 549
CviQI GTAC 1 cut(s) 20
DdeI CTNAG 2 cut(s) 54, 228
DpnI GATC 3 cut(s) 28, 44, 468
DpnII GATC 3 cut(s) 26, 42, 466
DraI TTTAAA 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 78
Eco147I AGGCCT 1 cut(s) 301
Eco47I GGWCC 2 cut(s) 75, 508
Eco88I CYCGRG 1 cut(s) 305
EcoO109I RGGNCCY 2 cut(s) 278, 508
EcoRII CCWGG 1 cut(s) 357
EcoT14I CCWWGG 1 cut(s) 78
EcoT22I ATGCAT 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 78
Esp3I CGTCTC 1 cut(s) 359
FaeI CATG 1 cut(s) 552
FaiI YATR 7 cut(s) 113, 117, 133, 342, 465, 519, 550
FaqI GGGAC 1 cut(s) 521
FatI CATG 1 cut(s) 548
Fnu4HI GCNGC 2 cut(s) 211, 425
FokI GGATG 1 cut(s) 498
Fsp4HI GCNGC 2 cut(s) 211, 425
FspBI CTAG 1 cut(s) 143
GluI GCNGC 2 cut(s) 211, 425
HaeIII GGCC 3 cut(s) 279, 301, 538
HapII CCGG 2 cut(s) 306, 388
Hin1II CATG 1 cut(s) 552
HpaII CCGG 2 cut(s) 306, 388
Hpy166II GTNNAC 1 cut(s) 20
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 5 cut(s) 56, 73, 91, 404, 549
Hpy8I GTNNAC 1 cut(s) 20
HpyAV CCTTC 2 cut(s) 269, 407
HpyCH4IV ACGT 2 cut(s) 16, 352
HpyCH4V TGCA 1 cut(s) 115
HpyF3I CTNAG 2 cut(s) 54, 228
HpySE526I ACGT 2 cut(s) 16, 352
Hsp92II CATG 1 cut(s) 552
KflI GGGWCCC 1 cut(s) 508
Kzo9I GATC 3 cut(s) 26, 42, 466
LmnI GCTCC 1 cut(s) 133
LweI GCATC 1 cut(s) 476
MaeI CTAG 1 cut(s) 143
MaeII ACGT 2 cut(s) 16, 352
MaeIII GTNAC 3 cut(s) 82, 100, 322
MalI GATC 3 cut(s) 28, 44, 468
MboI GATC 3 cut(s) 26, 42, 466
MboII GAAGA 1 cut(s) 392
MflI RGATCY 1 cut(s) 26
MnlI CCTC 3 cut(s) 296, 312, 476
Mph1103I ATGCAT 1 cut(s) 117
MseI TTAA 2 cut(s) 317, 336
MspI CCGG 2 cut(s) 306, 388
MspR9I CCNGG 4 cut(s) 306, 307, 359, 389
MvaI CCWGG 1 cut(s) 359
NciI CCSGG 3 cut(s) 306, 307, 389
NdeII GATC 3 cut(s) 26, 42, 466
NlaIII CATG 1 cut(s) 552
NlaIV GGNNCC 4 cut(s) 28, 95, 509, 510
NmuCI GTSAC 1 cut(s) 322
NsiI ATGCAT 1 cut(s) 117
PagI TCATGA 1 cut(s) 548
PceI AGGCCT 1 cut(s) 301
PflMI CCANNNNNTGG 1 cut(s) 104
PkrI GCNGC 2 cut(s) 212, 426
PpuMI RGGWCCY 1 cut(s) 508
Psp5II RGGWCCY 1 cut(s) 508
Psp6I CCWGG 1 cut(s) 357
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 4 cut(s) 28, 95, 509, 510
PspPI GGNCC 3 cut(s) 75, 278, 508
PspPPI RGGWCCY 1 cut(s) 508
PstNI CAGNNNCTG 1 cut(s) 104
PsuI RGATCY 1 cut(s) 26
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
SaqAI TTAA 2 cut(s) 317, 336
SatI GCNGC 2 cut(s) 211, 425
Sau3AI GATC 3 cut(s) 26, 42, 466
Sau96I GGNCC 3 cut(s) 75, 278, 508
ScrFI CCNGG 4 cut(s) 306, 307, 359, 389
SfaNI GCATC 1 cut(s) 476
SinI GGWCC 2 cut(s) 75, 508
SmaI CCCGGG 1 cut(s) 307
SseBI AGGCCT 1 cut(s) 301
SsiI CCGC 2 cut(s) 210, 424
SspMI CTAG 1 cut(s) 143
StuI AGGCCT 1 cut(s) 301
StyD4I CCNGG 4 cut(s) 304, 305, 357, 387
StyI CCWWGG 1 cut(s) 78
TaiI ACGT 2 cut(s) 19, 355
TaqI TCGA 1 cut(s) 366
TatI WGTACW 1 cut(s) 19
TauI GCSGC 2 cut(s) 213, 427
Tru1I TTAA 2 cut(s) 317, 336
Tru9I TTAA 2 cut(s) 317, 336
TseFI GTSAC 1 cut(s) 322
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 3 cut(s) 17, 320, 491
TspMI CCCGGG 1 cut(s) 305
Van91I CCANNNNNTGG 1 cut(s) 104
VpaK11BI GGWCC 2 cut(s) 75, 508
XmaI CCCGGG 1 cut(s) 305
XspI CTAG 1 cut(s) 143
Zsp2I ATGCAT 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.