Rroxscaffold_4G00293860

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0025470
Physical Location & Seq
Reverse (-)
14083282 .. 14085060
1779 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00293860.1

Sequence Viewer

Length: 513 bp
ATGTGGATTAAACGTTGCAATGATTTTGTCCTGACAATTTCGGCTGACCCAGTATCAAATCCAAACGATTGCATTATGCTAGTCATCTATGAGGAATGGTGTCAATTGGCTTTCATTAGACTTGGCCAAGACACAACGTGGACTTATGTGGATCCAAGTGTTTGGCCTCATGCCGGTGATAATCTTCTCGGGCTCTGTTCACTCACATTGGAGATAGATTATGAGTACTGCAAGAGAGCTATGTCCTTCCTCATCAACAACAGACTTAATTGCCCGAGATGCACCAACAACTACATGGATTTCAAAGAAGACGAGAACGACCTTGTGCTTCCGGTGGAAAGGGTGAAATCCAAAACCCTAGCAATGGTGATAGAGTGGTGCGAGAATCACGCCCCCGGCATGGAAAATGCACCACCCCGGAAGAACTCAAAGCATAGGATGCTGAATTCATCTAGGGACTCGACCCAGTTAACCTCTACCTCGCTGCCGAATGTCTTAAAATTAAGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

170

Amino Acids

19.66

Weight (kDa)

5.61

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025470)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000046.1_g000028
rosa_roxburghii Rroxscaffold_4G00293860
rosa_samantha Rh1BG272900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 13
AclWI GGATC 2 cut(s) 146, 159
AcoI YGGCCR 1 cut(s) 124
AcsI RAATTY 1 cut(s) 445
AdeI CACNNNGTG 1 cut(s) 138
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 3 cut(s) 173, 364, 400
AgsI TTSAA 1 cut(s) 304
AluBI AGCT 1 cut(s) 239
AluI AGCT 1 cut(s) 239
AlwI GGATC 2 cut(s) 146, 159
Ama87I CYCGRG 2 cut(s) 188, 274
AoxI GGCC 2 cut(s) 124, 164
ApeKI GCWGC 1 cut(s) 484
ApoI RAATTY 1 cut(s) 445
AsuC2I CCSGG 2 cut(s) 396, 418
AsuHPI GGTGA 3 cut(s) 188, 355, 379
AvaI CYCGRG 2 cut(s) 188, 274
BalI TGGCCA 1 cut(s) 126
BamHI GGATCC 1 cut(s) 151
BanII GRGCYC 1 cut(s) 195
BbsI GAAGAC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 471
BcnI CCSGG 2 cut(s) 396, 418
BfaI CTAG 3 cut(s) 80, 359, 453
BisI GCNGC 1 cut(s) 485
BlsI GCNGC 1 cut(s) 486
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 2 cut(s) 396, 418
BmeT110I CYCGRG 2 cut(s) 188, 274
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 2 cut(s) 396, 418
BmrI ACTGGG 2 cut(s) 44, 460
BmsI GCATC 2 cut(s) 269, 429
BmuI ACTGGG 2 cut(s) 44, 460
BpiI GAAGAC 1 cut(s) 315
BpuMI CCSGG 2 cut(s) 396, 418
BsaJI CCNNGG 2 cut(s) 394, 416
BsaWI WCCGGW 1 cut(s) 331
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 364, 400
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 2 cut(s) 50, 466
Bse3DI GCAATG 2 cut(s) 25, 369
BseDI CCNNGG 2 cut(s) 394, 416
BseGI GGATG 1 cut(s) 444
BseLI CCNNNNNNNGG 3 cut(s) 173, 364, 400
BseMI GCAATG 2 cut(s) 25, 369
BseNI ACTGG 2 cut(s) 50, 466
BseXI GCAGC 1 cut(s) 471
BshFI GGCC 2 cut(s) 126, 166
BsiHKCI CYCGRG 2 cut(s) 188, 274
BsiSI CCGG 4 cut(s) 174, 332, 396, 418
BslFI GGGAC 1 cut(s) 470
BslI CCNNNNNNNGG 3 cut(s) 173, 364, 400
BsmFI GGGAC 1 cut(s) 470
BsnI GGCC 2 cut(s) 126, 166
BsoBI CYCGRG 2 cut(s) 188, 274
Bsp1286I GDGCHC 1 cut(s) 195
Bsp143I GATC 1 cut(s) 151
BspANI GGCC 2 cut(s) 126, 166
BspLI GGNNCC 1 cut(s) 153
BspPI GGATC 2 cut(s) 146, 159
BsrDI GCAATG 2 cut(s) 25, 369
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 2 cut(s) 50, 466
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 394, 416
BssMI GATC 1 cut(s) 151
BstAPI GCANNNNNTGC 1 cut(s) 439
BstF5I GGATG 1 cut(s) 444
BstKTI GATC 1 cut(s) 154
BstMBI GATC 1 cut(s) 151
BstMWI GCNNNNNNNGC 2 cut(s) 279, 439
BstSCI CCNGG 2 cut(s) 394, 416
BstV1I GCAGC 1 cut(s) 471
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 1 cut(s) 151
BstXI CCANNNNNNTGG 1 cut(s) 162
BstYI RGATCY 1 cut(s) 151
BsuRI GGCC 2 cut(s) 126, 166
BtsCI GGATG 1 cut(s) 444
Cfr10I RCCGGY 1 cut(s) 173
Csp6I GTAC 1 cut(s) 226
CviAII CATG 3 cut(s) 170, 295, 400
CviJI RGCY 6 cut(s) 44, 110, 126, 166, 193, 239
CviKI_1 RGCY 6 cut(s) 44, 110, 126, 166, 193, 239
CviQI GTAC 1 cut(s) 226
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
DraIII CACNNNGTG 1 cut(s) 138
EaeI YGGCCR 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 195
Eco88I CYCGRG 2 cut(s) 188, 274
EcoRI GAATTC 1 cut(s) 445
EcoT38I GRGCYC 1 cut(s) 195
FaeI CATG 3 cut(s) 173, 298, 403
FaiI YATR 9 cut(s) 77, 90, 147, 171, 222, 242, 296, 401, 435
FaqI GGGAC 1 cut(s) 470
FatI CATG 3 cut(s) 169, 294, 399
Fnu4HI GCNGC 1 cut(s) 485
FokI GGATG 1 cut(s) 451
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 1 cut(s) 485
FspBI CTAG 3 cut(s) 80, 359, 453
GluI GCNGC 1 cut(s) 485
HaeIII GGCC 2 cut(s) 126, 166
HapII CCGG 4 cut(s) 174, 332, 396, 418
Hin1II CATG 3 cut(s) 173, 298, 403
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
HinfI GANTC 2 cut(s) 385, 458
HpaI GTTAAC 1 cut(s) 471
HpaII CCGG 4 cut(s) 174, 332, 396, 418
HphI GGTGA 3 cut(s) 188, 355, 379
Hpy166II GTNNAC 3 cut(s) 141, 200, 471
Hpy188III TCNNGA 1 cut(s) 31
Hpy8I GTNNAC 3 cut(s) 141, 200, 471
HpyAV CCTTC 1 cut(s) 256
HpyCH4IV ACGT 2 cut(s) 13, 137
HpyCH4V TGCA 5 cut(s) 18, 72, 231, 282, 410
HpyF10VI GCNNNNNNNGC 2 cut(s) 279, 439
HpySE526I ACGT 2 cut(s) 13, 137
Hsp92II CATG 3 cut(s) 173, 298, 403
KspAI GTTAAC 1 cut(s) 471
Kzo9I GATC 1 cut(s) 151
LpnPI CCDG 7 cut(s) 44, 63, 187, 345, 409, 431, 479
Lsp1109I GCAGC 1 cut(s) 471
LweI GCATC 2 cut(s) 269, 429
MaeI CTAG 3 cut(s) 80, 359, 453
MaeII ACGT 2 cut(s) 13, 137
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 3 cut(s) 176, 320, 433
MfeI CAATTG 1 cut(s) 104
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 195
MlsI TGGCCA 1 cut(s) 126
MluCI AATT 6 cut(s) 36, 104, 268, 445, 500, 508
MluNI TGGCCA 1 cut(s) 126
MlyI GAGTC 1 cut(s) 452
MnlI CCTC 5 cut(s) 85, 177, 260, 484, 490
Mox20I TGGCCA 1 cut(s) 126
MscI TGGCCA 1 cut(s) 126
MseI TTAA 5 cut(s) 9, 267, 470, 497, 503
MslI CAYNNNNRTG 1 cut(s) 174
Msp20I TGGCCA 1 cut(s) 126
MspI CCGG 4 cut(s) 174, 332, 396, 418
MspR9I CCNGG 2 cut(s) 396, 418
MunI CAATTG 1 cut(s) 104
MwoI GCNNNNNNNGC 2 cut(s) 279, 439
NciI CCSGG 2 cut(s) 396, 418
NdeII GATC 1 cut(s) 151
NlaIII CATG 3 cut(s) 173, 298, 403
NlaIV GGNNCC 1 cut(s) 153
PfeI GAWTC 1 cut(s) 385
PkrI GCNGC 1 cut(s) 486
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
Psp1406I AACGTT 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 153
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
RseI CAYNNNNRTG 1 cut(s) 174
SaqAI TTAA 5 cut(s) 9, 267, 470, 497, 503
SatI GCNGC 1 cut(s) 485
Sau3AI GATC 1 cut(s) 151
ScaI AGTACT 1 cut(s) 227
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 2 cut(s) 396, 418
SduI GDGCHC 1 cut(s) 195
SetI ASST 6 cut(s) 16, 140, 241, 324, 476, 482
SfaNI GCATC 2 cut(s) 269, 429
SmiMI CAYNNNNRTG 1 cut(s) 174
Sse9I AATT 6 cut(s) 36, 104, 268, 445, 500, 508
SspMI CTAG 3 cut(s) 80, 359, 453
StyD4I CCNGG 2 cut(s) 394, 416
TaiI ACGT 2 cut(s) 16, 140
TaqI TCGA 1 cut(s) 461
TasI AATT 6 cut(s) 36, 104, 268, 445, 500, 508
TatI WGTACW 1 cut(s) 225
TfiI GAWTC 1 cut(s) 385
Tru1I TTAA 5 cut(s) 9, 267, 470, 497, 503
Tru9I TTAA 5 cut(s) 9, 267, 470, 497, 503
TseI GCWGC 1 cut(s) 484
TspDTI ATGAA 2 cut(s) 103, 438
XapI RAATTY 1 cut(s) 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
XspI CTAG 3 cut(s) 80, 359, 453
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.