Rroxscaffold_5G00347850

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0019192
Physical Location & Seq
Reverse (-)
19523130 .. 19528745
5616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00347850.1

Sequence Viewer

Length: 2412 bp
ATGGCCGACTCAGTGGTACAGGGGAAACTGGGTTTAGAATTTAATTTGCTGTATGAGGCCGTCACACAAGTAGTGAAGGACAAGACTAAGATGTTCAGAAGCGTCTTCGAAGAGCTAAAACTCACGCTGGACTCTTTACAACCATTGGTCAAAGAGAAGGTACAACGTGACATATCTGATCAGTTCCTTACTGAAGAACTTGAAATATTTAGAAAAAAATTGGAAAATGGGGTAGAGCTAGTTCGTAAGTCCTCGAACTTAAGTGTTTGGAGCGCCTACAGAAAGTATCATTACAGACACAAACTTATTGCATTGGAAAAATCTCTTCAAAGTCTGTTAGATATACTGAGAATTCAGGCACTGATGGATGTGGGGAAAACTTGGAATTCGGTAAACAATAGAGAGACGATGGTCAAGGAAATGACTTATTGGAATACAGAACCTGAACCCCCATCTTTTACGGTTGGGTTGGATGAGCCTTTAGAGGAAGTGAAGAAGATGCTTCTTACCGAAGATATGTCAGTTCTTGTCGTCACTGGTCCTGGAGGATGTGGGAAAACCACCTTGGCAACAACCATTTGTCGAGATGCCAAAATCAAAGAGAAATTCAGGGACAATATTTTCTTTGTCACGGTTGGAAAAAGACCAAGTATGAGCTTGATTTTGCAACAACTAAATCAACCAATGAGTTCTGAGACACCTAATATCCAAGACGAAGCAATTGCAGATTGGTTGCGTGTCTTTCTAAAGGAACAAGGGCAGAATGTGCTGTTGATCTTGGATGATGTTTGGTCTGGATCAGAATCACTTCTTGAAAAGTTCATCGTAGATGGAGGGAAAGAAACGTCAAATTATAAAATTTTGGTGACGTCCAGATCTGAAATTCCACGATTAGGTTCTTCATATCACCTAAATTTATTGAATGATGAAGATTCAATGACTCTTTTCCGTCATTCAGCATTCCTTGGAGACCGGAGCATCCATATTCCACTTGATCTTTCGAGAGAGATCGTAAAGAACTGTAAGGGACTTCCACTTGCCATTCTAGTGGTTGGAAAATCGCTTTGCGGAAAGCCTATAGAAATCTGGCGAAACACACTATTGAAATGGTCTAAAGGTTATTCTATTCTCGATTATGAGAATGCTTTGCTGTTTAGACTCCAAAGCAGCTTAGATGACTTGGGTAAAGAAAACTATATCCTCCTAGAATCTTTCATTGATCTTGCTTCATTCCCTGAAGACCAAGCAATCCCTGTCCCCGCCCTCGTTGATATGTGGGCAGAGCTATATGAGCTACGTGAAGATATAATGTGCATTGCGAATCTCTATGAGCTCACCACCCGCAGTTTGGCCAATGTTGTAGTGAGAAGAAAGGTGGAGGAGGACTACAGTGAACACTTTGTTACCCAGCATGATATGCTTAGAAGCCTAGCAATCTATCAGACTCGACATGATCCAGTCCTAAGCAAAAGACTGAATATAGACATTCGTGGAGACGATTTTCCCAAGTGGTGGACAGAACAGACCAAAAAACCAAAGAAAACTCGCTTATTATCTATCTCAACTGATGAGGTGTTCTCCACGAAATGGCCCATCATGCAACTACCAATAGCTGAGGTTCTGGTTCTGAATTTTCGAACAAAGAACTATGTCTTGCCTAAGTTCATCAGGGATATGTACAAATCGTTGAAGGTTCTGATAGTCATAAATTATGGGTCCGCAATTGCTGAAGTAAGTGATTTTCACCTGTTGAGTTCGTTATCAAATCTGAAAACAATCAGATTGCAGCGGATTTCAATTCCTTCCGTTACTAAGAACTCCATACACTTGAAGAGTCTCCAGAAAATATCTGTTTTCATGTGTAACATCAGCCAAGCTTTTGGGAAGGATGCTATCCCAATTTCGCATGCATTGCCAATTCTAGTGGAAATAAACATCGACTATTGCAGTGATTTGGTAGAATTGCAGGACGACGTTTGTAATCTTTTTCACCTAGAGAAGCTCAGCATCACCAATTGCCACAAGTTATCTGCCTTACCTGAAGAAATTGGGAAGTTGCTCAAGTTGGAAGTGTTGAGGCTAAGGTCATGCACTGACTTGGTAGAATTGCCAGGCTCCATGGGGAACCTAGAGAAGCTAAACTTTCTTGACATATCCCATTGCTTCAGCATTAAGGAGTTGCCCGAAGAAATTGGTAAATTGGGCAGTTTAAGAAAGCTGAACATGAGAGAGTGCTCGAGATTGGAAGAGTTGCCGGAATCATTTTCAGACCTCGAGGAGTTGAAGGAAGTGATATGTGACAAATATACACAAGGTTTGTGGGAGCCCTTCTTACCCACACTCAGAAGCACGGAAATAAGGGTGGTCAAAGAAGCAATCAACCTAAATTGGCTCCAGAAGCTTCAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

803

Amino Acids

92.18

Weight (kDa)

5.93

Isoelectric Point (pI)

45.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 9 - 127 1.7e-18 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 159 - 321 2e-22 NB-ARC domain
AAA_22 PF13401 174 - 263 1.6e-06 AAA domain
NACHT PF05729 175 - 263 8.3e-07 NACHT domain
LRR_14 PF23598 645 - 721 1.5e-10 Leucine-rich repeat region
LRR_14 PF23598 716 - 771 6.4e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019192)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15390 FvH4_1g15390 FvH4_1g15390 FvH4_1g15390 FvH4_1g15390 FvH4_1g15390 FvH4_1g15390
rosa_roxburghii Rroxscaffold_5G00347850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 855
AatII GACGTC 1 cut(s) 872
AbsI CCTCGAGG 1 cut(s) 2273
AccB7I CCANNNNNTGG 2 cut(s) 1512, 1587
AciI CCGC 5 cut(s) 1068, 1260, 1342, 1719, 1789
AclWI GGATC 2 cut(s) 805, 1448
AcoI YGGCCR 2 cut(s) 3, 1350
AcsI RAATTY 8 cut(s) 38, 351, 385, 605, 858, 882, 913, 1630
AcuI CTGAAG 6 cut(s) 213, 1257, 1749, 2061, 2149, 2387
AcyI GRCGYC 1 cut(s) 869
AfaI GTAC 3 cut(s) 18, 162, 1679
AfiI CCNNNNNNNGG 4 cut(s) 893, 1348, 1512, 1587
AflII CTTAAG 1 cut(s) 259
AjnI CCWGG 2 cut(s) 541, 2110
AjuI GAANNNNNNNTTGG 2 cut(s) 548, 580
Alw21I GWGCWC 2 cut(s) 1335, 2237
Alw26I GTCTC 5 cut(s) 398, 689, 963, 1488, 1841
AlwI GGATC 2 cut(s) 805, 1448
AlwNI CAGNNNCTG 2 cut(s) 361, 443
Ama87I CYCGRG 2 cut(s) 2236, 2273
AoxI GGCC 4 cut(s) 3, 57, 1350, 1589
ApeKI GCWGC 2 cut(s) 1167, 1786
ApoI RAATTY 8 cut(s) 38, 351, 385, 605, 858, 882, 913, 1630
Asp700I GAANNNNTTC 1 cut(s) 807
AspLEI GCGC 1 cut(s) 275
AspS9I GGNCC 3 cut(s) 539, 1590, 1716
AsuHPI GGTGA 6 cut(s) 877, 899, 1327, 1736, 1982, 2002
AsuII TTCGAA 2 cut(s) 108, 1636
AvaI CYCGRG 2 cut(s) 2236, 2273
AvaII GGWCC 2 cut(s) 539, 1716
BalI TGGCCA 1 cut(s) 1352
BanII GRGCYC 2 cut(s) 1335, 2328
BbsI GAAGAC 2 cut(s) 97, 1245
Bbv12I GWGCWC 2 cut(s) 1335, 2237
BbvCI CCTCAGC 1 cut(s) 1614
BbvI GCAGC 2 cut(s) 1179, 1798
BccI CCATC 5 cut(s) 358, 403, 460, 824, 1601
BceAI ACGGC 1 cut(s) 44
BcgI CGANNNNNNTGC 2 cut(s) 704, 738
BciT130I CCWGG 2 cut(s) 543, 2112
BclI TGATCA 1 cut(s) 178
BcoDI GTCTC 5 cut(s) 398, 689, 963, 1488, 1841
BfaI CTAG 7 cut(s) 239, 1046, 1205, 1430, 1922, 1994, 2129
BfmI CTRYAG 3 cut(s) 277, 1077, 1387
BfoI RGCGCY 1 cut(s) 276
BfrI CTTAAG 1 cut(s) 259
BglII AGATCT 1 cut(s) 875
BisI GCNGC 2 cut(s) 1168, 1787
BlpI GCTNAGC 1 cut(s) 2003
BlsI GCNGC 2 cut(s) 1169, 1788
Bme1390I CCNGG 2 cut(s) 543, 2112
Bme18I GGWCC 2 cut(s) 539, 1716
BmeT110I CYCGRG 2 cut(s) 2236, 2273
BmgT120I GGNCC 3 cut(s) 539, 1590, 1716
BmiI GGNNCC 5 cut(s) 1717, 2116, 2126, 2325, 2393
BmrFI CCNGG 2 cut(s) 543, 2112
BmrI ACTGGG 1 cut(s) 38
BmsI GCATC 5 cut(s) 489, 577, 987, 1879, 2016
BmuI ACTGGG 1 cut(s) 38
BoxI GACNNNNGTC 1 cut(s) 410
BpiI GAAGAC 2 cut(s) 97, 1245
BplI GAGNNNNNCTC 2 cut(s) 1562, 1594
BpmI CTGGAG 3 cut(s) 564, 1823, 2378
Bpu10I CCTNAGC 3 cut(s) 1463, 1614, 2081
Bpu1102I GCTNAGC 1 cut(s) 2003
Bpu14I TTCGAA 2 cut(s) 108, 1636
BpuEI CTTGAG 1 cut(s) 2045
BsaAI YACGTR 1 cut(s) 1298
BsaBI GATNNNNATC 1 cut(s) 802
BsaHI GRCGYC 1 cut(s) 869
BsaI GGTCTC 1 cut(s) 963
BsaJI CCNNGG 3 cut(s) 564, 964, 2118
BsaWI WCCGGW 1 cut(s) 972
Bsc4I CCNNNNNNNGG 4 cut(s) 893, 1348, 1512, 1587
Bse1I ACTGG 3 cut(s) 33, 541, 1457
Bse3DI GCAATG 3 cut(s) 1314, 1910, 2158
Bse8I GATNNNNATC 1 cut(s) 802
BseBI CCWGG 2 cut(s) 543, 2112
BseDI CCNNGG 3 cut(s) 564, 964, 2118
BseGI GGATG 6 cut(s) 373, 478, 554, 787, 978, 1894
BseJI GATNNNNATC 1 cut(s) 802
BseLI CCNNNNNNNGG 4 cut(s) 893, 1348, 1512, 1587
BseMI GCAATG 3 cut(s) 1314, 1910, 2158
BseMII CTCAG 6 cut(s) 24, 338, 684, 1605, 2017, 2356
BseNI ACTGG 3 cut(s) 33, 541, 1457
BseRI GAGGAG 2 cut(s) 1394, 2291
BseXI GCAGC 2 cut(s) 1179, 1798
BseYI CCCAGC 1 cut(s) 1407
BshFI GGCC 4 cut(s) 5, 59, 1352, 1591
BsiHKAI GWGCWC 2 cut(s) 1335, 2237
BsiHKCI CYCGRG 2 cut(s) 2236, 2273
BsiSI CCGG 2 cut(s) 973, 2255
BslFI GGGAC 3 cut(s) 626, 1041, 1241
BslI CCNNNNNNNGG 4 cut(s) 893, 1348, 1512, 1587
BsmAI GTCTC 5 cut(s) 398, 689, 963, 1488, 1841
BsmBI CGTCTC 2 cut(s) 398, 1488
BsmFI GGGAC 3 cut(s) 626, 1041, 1241
BsmI GAATGC 2 cut(s) 959, 1147
BsnI GGCC 4 cut(s) 5, 59, 1352, 1591
Bso31I GGTCTC 1 cut(s) 963
BsoBI CYCGRG 2 cut(s) 2236, 2273
Bsp119I TTCGAA 2 cut(s) 108, 1636
Bsp1286I GDGCHC 3 cut(s) 1335, 2237, 2328
Bsp1407I TGTACA 1 cut(s) 1677
Bsp143I GATC 8 cut(s) 178, 774, 797, 875, 994, 1008, 1219, 1453
Bsp1720I GCTNAGC 1 cut(s) 2003
Bsp19I CCATGG 1 cut(s) 2118
BspACI CCGC 5 cut(s) 1068, 1260, 1342, 1719, 1789
BspANI GGCC 4 cut(s) 5, 59, 1352, 1591
BspCNI CTCAG 6 cut(s) 23, 339, 685, 1606, 2016, 2355
BspLI GGNNCC 5 cut(s) 1717, 2116, 2126, 2325, 2393
BspPI GGATC 2 cut(s) 805, 1448
BspQI GCTCTTC 1 cut(s) 105
BspT104I TTCGAA 2 cut(s) 108, 1636
BspTI CTTAAG 1 cut(s) 259
BspTNI GGTCTC 1 cut(s) 963
BsrDI GCAATG 3 cut(s) 1314, 1910, 2158
BsrGI TGTACA 1 cut(s) 1677
BsrI ACTGG 3 cut(s) 33, 541, 1457
BssECI CCNNGG 3 cut(s) 564, 964, 2118
BssMI GATC 8 cut(s) 178, 774, 797, 875, 994, 1008, 1219, 1453
BssNI GRCGYC 1 cut(s) 869
BssT1I CCWWGG 3 cut(s) 564, 964, 2118
Bst2UI CCWGG 2 cut(s) 543, 2112
Bst4CI ACNGT 4 cut(s) 463, 634, 1022, 1391
Bst6I CTCTTC 4 cut(s) 105, 330, 1826, 2241
BstACI GRCGYC 1 cut(s) 869
BstAFI CTTAAG 1 cut(s) 259
BstAPI GCANNNNNTGC 3 cut(s) 766, 1417, 1912
BstAUI TGTACA 1 cut(s) 1677
BstBAI YACGTR 1 cut(s) 1298
BstBI TTCGAA 2 cut(s) 108, 1636
BstC8I GCNNGC 1 cut(s) 1908
BstDSI CCRYGG 1 cut(s) 2118
BstF5I GGATG 6 cut(s) 373, 478, 554, 787, 978, 1894
BstH2I RGCGCY 1 cut(s) 276
BstHHI GCGC 1 cut(s) 275
BstKTI GATC 8 cut(s) 181, 777, 800, 878, 997, 1011, 1222, 1456
BstMAI GTCTC 5 cut(s) 398, 689, 963, 1488, 1841
BstMBI GATC 8 cut(s) 178, 774, 797, 875, 994, 1008, 1219, 1453
BstMWI GCNNNNNNNGC 6 cut(s) 766, 1291, 1417, 1597, 1912, 2398
BstNI CCWGG 2 cut(s) 543, 2112
BstNSI RCATGY 1 cut(s) 1910
BstPAI GACNNNNGTC 1 cut(s) 410
BstSCI CCNGG 2 cut(s) 541, 2110
BstSFI CTRYAG 3 cut(s) 277, 1077, 1387
BstV1I GCAGC 2 cut(s) 1179, 1798
BstV2I GAAGAC 2 cut(s) 97, 1245
BstX2I RGATCY 1 cut(s) 875
BstXI CCANNNNNNTGG 2 cut(s) 1048, 1880
BstYI RGATCY 1 cut(s) 875
BsuRI GGCC 4 cut(s) 5, 59, 1352, 1591
BtgI CCRYGG 1 cut(s) 2118
BtsCI GGATG 6 cut(s) 373, 478, 554, 787, 978, 1894
BtsI GCAGTG 1 cut(s) 1954
BtsIMutI CAGTG 6 cut(s) 18, 359, 534, 1396, 1954, 2091
Cac8I GCNNGC 1 cut(s) 1908
CaiI CAGNNNCTG 2 cut(s) 361, 443
CfoI GCGC 1 cut(s) 275
Cfr13I GGNCC 3 cut(s) 539, 1590, 1716
CseI GACGC 1 cut(s) 91
Csp6I GTAC 3 cut(s) 17, 161, 1678
CspCI CAANNNNNGTGG 4 cut(s) 1905, 1940, 2300, 2335
CviAII CATG 8 cut(s) 1412, 1451, 1597, 1858, 1907, 2088, 2119, 2224
CviQI GTAC 3 cut(s) 17, 161, 1678
DpnI GATC 8 cut(s) 180, 776, 799, 877, 996, 1010, 1221, 1455
DpnII GATC 8 cut(s) 178, 774, 797, 875, 994, 1008, 1219, 1453
EaeI YGGCCR 2 cut(s) 3, 1350
Eam1104I CTCTTC 4 cut(s) 105, 330, 1826, 2241
EarI CTCTTC 4 cut(s) 105, 330, 1826, 2241
Ecl136II GAGCTC 1 cut(s) 1333
Eco130I CCWWGG 3 cut(s) 564, 964, 2118
Eco24I GRGCYC 2 cut(s) 1335, 2328
Eco31I GGTCTC 1 cut(s) 963
Eco47I GGWCC 2 cut(s) 539, 1716
Eco53kI GAGCTC 1 cut(s) 1333
Eco57I CTGAAG 6 cut(s) 213, 1257, 1749, 2061, 2149, 2387
Eco88I CYCGRG 2 cut(s) 2236, 2273
EcoICRI GAGCTC 1 cut(s) 1333
EcoRI GAATTC 2 cut(s) 351, 385
EcoRII CCWGG 2 cut(s) 541, 2110
EcoT14I CCWWGG 3 cut(s) 564, 964, 2118
EcoT22I ATGCAT 1 cut(s) 1912
EcoT38I GRGCYC 2 cut(s) 1335, 2328
ErhI CCWWGG 3 cut(s) 564, 964, 2118
Esp3I CGTCTC 2 cut(s) 398, 1488
FaeI CATG 8 cut(s) 1415, 1454, 1600, 1861, 1910, 2091, 2122, 2227
FalI AAGNNNNNCTT 2 cut(s) 2126, 2158
FaqI GGGAC 3 cut(s) 626, 1041, 1241
FatI CATG 8 cut(s) 1411, 1450, 1596, 1857, 1906, 2087, 2118, 2223
FauI CCCGC 2 cut(s) 1267, 1349
FbaI TGATCA 1 cut(s) 178
Fnu4HI GCNGC 2 cut(s) 1168, 1787
FokI GGATG 6 cut(s) 380, 485, 561, 794, 965, 1901
FriOI GRGCYC 2 cut(s) 1335, 2328
Fsp4HI GCNGC 2 cut(s) 1168, 1787
FspBI CTAG 7 cut(s) 239, 1046, 1205, 1430, 1922, 1994, 2129
GlaI GCGC 1 cut(s) 274
GluI GCNGC 2 cut(s) 1168, 1787
GsaI CCCAGC 1 cut(s) 1411
GsuI CTGGAG 3 cut(s) 564, 1823, 2378
HaeII RGCGCY 1 cut(s) 276
HaeIII GGCC 4 cut(s) 5, 59, 1352, 1591
HapII CCGG 2 cut(s) 973, 2255
HgaI GACGC 1 cut(s) 91
HhaI GCGC 1 cut(s) 275
Hin1I GRCGYC 1 cut(s) 869
Hin1II CATG 8 cut(s) 1415, 1454, 1600, 1861, 1910, 2091, 2122, 2227
Hin6I GCGC 1 cut(s) 273
HinP1I GCGC 1 cut(s) 273
HindIII AAGCTT 2 cut(s) 1875, 2399
HpaII CCGG 2 cut(s) 973, 2255
HphI GGTGA 6 cut(s) 877, 899, 1327, 1736, 1982, 2002
Hpy166II GTNNAC 3 cut(s) 394, 1394, 1515
Hpy8I GTNNAC 3 cut(s) 394, 1394, 1515
Hpy99I CGWCG 1 cut(s) 1976
HpyAV CCTTC 7 cut(s) 70, 151, 1684, 1812, 1879, 2278, 2338
HpyCH4III ACNGT 4 cut(s) 463, 634, 1022, 1391
HpyCH4IV ACGT 5 cut(s) 166, 845, 869, 1297, 1974
HpyF10VI GCNNNNNNNGC 6 cut(s) 766, 1291, 1417, 1597, 1912, 2398
HpySE526I ACGT 5 cut(s) 166, 845, 869, 1297, 1974
Hsp92I GRCGYC 1 cut(s) 869
Hsp92II CATG 8 cut(s) 1415, 1454, 1600, 1861, 1910, 2091, 2122, 2227
HspAI GCGC 1 cut(s) 273
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 8 cut(s) 178, 774, 797, 875, 994, 1008, 1219, 1453
LguI GCTCTTC 1 cut(s) 105
LmnI GCTCC 5 cut(s) 270, 975, 2120, 2323, 2397
Lsp1109I GCAGC 2 cut(s) 1179, 1798
LweI GCATC 5 cut(s) 489, 577, 987, 1879, 2016
MaeI CTAG 7 cut(s) 239, 1046, 1205, 1430, 1922, 1994, 2129
MaeII ACGT 5 cut(s) 166, 845, 869, 1297, 1974
MaeIII GTNAC 9 cut(s) 61, 167, 532, 628, 865, 1402, 1807, 1862, 2297
MalI GATC 8 cut(s) 180, 776, 799, 877, 996, 1010, 1221, 1455
MboI GATC 8 cut(s) 178, 774, 797, 875, 994, 1008, 1219, 1453
MfeI CAATTG 3 cut(s) 720, 1722, 2014
MflI RGATCY 1 cut(s) 875
MhlI GDGCHC 3 cut(s) 1335, 2237, 2328
MlsI TGGCCA 1 cut(s) 1352
MluNI TGGCCA 1 cut(s) 1352
MlyI GAGTC 6 cut(s) 2, 125, 934, 1152, 1438, 1843
MmeI TCCRAC 4 cut(s) 450, 616, 1033, 2046
Mox20I TGGCCA 1 cut(s) 1352
Mph1103I ATGCAT 1 cut(s) 1912
MroXI GAANNNNTTC 1 cut(s) 807
MscI TGGCCA 1 cut(s) 1352
MseI TTAA 4 cut(s) 42, 260, 2172, 2210
MslI CAYNNNNRTG 1 cut(s) 1046
Msp20I TGGCCA 1 cut(s) 1352
MspA1I CMGCKG 1 cut(s) 1789
MspCI CTTAAG 1 cut(s) 259
MspI CCGG 2 cut(s) 973, 2255
MspR9I CCNGG 2 cut(s) 543, 2112
MunI CAATTG 3 cut(s) 720, 1722, 2014
Mva1269I GAATGC 2 cut(s) 959, 1147
MvaI CCWGG 2 cut(s) 543, 2112
MwoI GCNNNNNNNGC 6 cut(s) 766, 1291, 1417, 1597, 1912, 2398
NcoI CCATGG 1 cut(s) 2118
NdeII GATC 8 cut(s) 178, 774, 797, 875, 994, 1008, 1219, 1453
NlaIII CATG 8 cut(s) 1415, 1454, 1600, 1861, 1910, 2091, 2122, 2227
NlaIV GGNNCC 5 cut(s) 1717, 2116, 2126, 2325, 2393
NmuCI GTSAC 6 cut(s) 61, 167, 532, 628, 865, 2297
NsiI ATGCAT 1 cut(s) 1912
NspI RCATGY 1 cut(s) 1910
NspV TTCGAA 2 cut(s) 108, 1636
PaeI GCATGC 1 cut(s) 1910
PaeR7I CTCGAG 2 cut(s) 2236, 2273
PciSI GCTCTTC 1 cut(s) 105
PctI GAATGC 2 cut(s) 959, 1147
PdmI GAANNNNTTC 1 cut(s) 807
PfeI GAWTC 5 cut(s) 803, 932, 1208, 1321, 2258
PflMI CCANNNNNTGG 2 cut(s) 1512, 1587
PfoI TCCNGGA 1 cut(s) 541
PkrI GCNGC 2 cut(s) 1169, 1788
PleI GAGTC 6 cut(s) 2, 125, 934, 1152, 1438, 1842
PpsI GAGTC 6 cut(s) 2, 125, 934, 1152, 1438, 1842
Ppu21I YACGTR 1 cut(s) 1298
PshAI GACNNNNGTC 1 cut(s) 410
PsiI TTATAA 1 cut(s) 855
Psp124BI GAGCTC 1 cut(s) 1335
Psp6I CCWGG 2 cut(s) 541, 2110
PspFI CCCAGC 1 cut(s) 1407
PspGI CCWGG 2 cut(s) 541, 2110
PspN4I GGNNCC 5 cut(s) 1717, 2116, 2126, 2325, 2393
PspPI GGNCC 3 cut(s) 539, 1590, 1716
PspXI VCTCGAGB 1 cut(s) 2273
PsrI GAACNNNNNNTAC 2 cut(s) 225, 257
PstNI CAGNNNCTG 2 cut(s) 361, 443
PsuI RGATCY 1 cut(s) 875
RsaI GTAC 3 cut(s) 18, 162, 1679
RsaNI GTAC 3 cut(s) 17, 161, 1678
RseI CAYNNNNRTG 1 cut(s) 1046
SacI GAGCTC 1 cut(s) 1335
SapI GCTCTTC 1 cut(s) 105
SaqAI TTAA 4 cut(s) 42, 260, 2172, 2210
SatI GCNGC 2 cut(s) 1168, 1787
Sau3AI GATC 8 cut(s) 178, 774, 797, 875, 994, 1008, 1219, 1453
Sau96I GGNCC 3 cut(s) 539, 1590, 1716
SchI GAGTC 6 cut(s) 2, 125, 934, 1152, 1438, 1843
ScrFI CCNGG 2 cut(s) 543, 2112
SduI GDGCHC 3 cut(s) 1335, 2237, 2328
SfaNI GCATC 5 cut(s) 489, 577, 987, 1879, 2016
SfcI CTRYAG 3 cut(s) 277, 1077, 1387
Sfr274I CTCGAG 2 cut(s) 2236, 2273
SfuI TTCGAA 2 cut(s) 108, 1636
SinI GGWCC 2 cut(s) 539, 1716
SlaI CTCGAG 2 cut(s) 2236, 2273
SmiMI CAYNNNNRTG 1 cut(s) 1046
SmlI CTYRAG 4 cut(s) 259, 2060, 2236, 2273
SmoI CTYRAG 4 cut(s) 259, 2060, 2236, 2273
SphI GCATGC 1 cut(s) 1910
SsiI CCGC 5 cut(s) 1068, 1260, 1342, 1719, 1789
SspI AATATT 2 cut(s) 207, 619
SspMI CTAG 7 cut(s) 239, 1046, 1205, 1430, 1922, 1994, 2129
SstI GAGCTC 1 cut(s) 1335
StyD4I CCNGG 2 cut(s) 541, 2110
StyI CCWWGG 3 cut(s) 564, 964, 2118
TaaI ACNGT 4 cut(s) 463, 634, 1022, 1391
TaiI ACGT 5 cut(s) 169, 848, 872, 1300, 1977
TatI WGTACW 1 cut(s) 1677
TfiI GAWTC 5 cut(s) 803, 932, 1208, 1321, 2258
Tru1I TTAA 4 cut(s) 42, 260, 2172, 2210
Tru9I TTAA 4 cut(s) 42, 260, 2172, 2210
TscAI CASTG 6 cut(s) 18, 366, 541, 1396, 1954, 2098
TseFI GTSAC 6 cut(s) 61, 167, 532, 628, 865, 2297
TseI GCWGC 2 cut(s) 1167, 1786
Tsp45I GTSAC 6 cut(s) 61, 167, 532, 628, 865, 2297
TspDTI ATGAA 7 cut(s) 811, 891, 942, 1204, 1218, 1654, 1846
TspGWI ACGGA 3 cut(s) 938, 1795, 2366
TspRI CASTG 6 cut(s) 18, 366, 541, 1396, 1954, 2098
Van91I CCANNNNNTGG 2 cut(s) 1512, 1587
Vha464I CTTAAG 1 cut(s) 259
VpaK11BI GGWCC 2 cut(s) 539, 1716
XapI RAATTY 8 cut(s) 38, 351, 385, 605, 858, 882, 913, 1630
XceI RCATGY 1 cut(s) 1910
XcmI CCANNNNNNNNNTGG 1 cut(s) 1345
XhoI CTCGAG 2 cut(s) 2236, 2273
XmnI GAANNNNTTC 1 cut(s) 807
XspI CTAG 7 cut(s) 239, 1046, 1205, 1430, 1922, 1994, 2129
ZraI GACGTC 1 cut(s) 870
Zsp2I ATGCAT 1 cut(s) 1912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.