Rroxscaffold_5G00353110

Phosphoenolpyruvate phosphomutase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0031357
Physical Location & Seq
Forward (+)
30657923 .. 30659480
1558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00353110.1

Sequence Viewer

Length: 435 bp
ATGGGAAGCCCCAACAAGTCAAATGCGTCTCCTTTTCTTACCACAGGAGAATCAGCGCCGCCGCCCGAGTCTCCGGCCAAAGCGCTCCGCCGGATTCTGGAGTTGCCAGGGGTTCACCTAGGCCCTGCTTGCTTTAATGGTCTCAGCGCCAAGCTTATTGAAAGAGCTGGCTTCCAGTATTGCTTCACCAGTGGATTTTCGATATCAGCTGCTACATTAGGGCTGCCAGATACAGGTTTTCTATCCTATGGAGAAATGGTGGATCAGGGGCAACAAATTACCCAAGCTGTGTCAATTCCTGTCATTGGGGATGGCGACAACGGTTACGGGAATGCAATGAATGTCAAGAGAACTGTCAAGGGATATATTAGAGCTGGCTTTGCTGGGATTCTGCTTGAAGATCAGGTCATTATTGAAATTTACTTGGATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEP_mutase

Protein Analysis

144

Amino Acids

15.18

Weight (kDa)

5.02

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEP_mutase PF13714 41 - 135 2.2e-21 Phosphoenolpyruvate phosphomutase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031357)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0005878.1_g000011
rosa_roxburghii Rroxscaffold_5G00353110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 59, 62, 88
AclWI GGATC 1 cut(s) 270
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 1 cut(s) 417
AfeI AGCGCT 1 cut(s) 84
AfiI CCNNNNNNNGG 3 cut(s) 97, 233, 305
AgsI TTSAA 3 cut(s) 161, 398, 416
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 5 cut(s) 154, 167, 209, 287, 374
AluI AGCT 5 cut(s) 154, 167, 209, 287, 374
Alw26I GTCTC 3 cut(s) 33, 75, 146
AlwI GGATC 1 cut(s) 270
Ama87I CYCGRG 1 cut(s) 65
Aor51HI AGCGCT 1 cut(s) 84
AoxI GGCC 2 cut(s) 75, 121
ApeKI GCWGC 2 cut(s) 209, 223
ApoI RAATTY 1 cut(s) 417
AspA2I CCTAGG 1 cut(s) 118
AspLEI GCGC 3 cut(s) 58, 85, 149
AspS9I GGNCC 1 cut(s) 122
AsuHPI GGTGA 2 cut(s) 107, 178
AvaI CYCGRG 1 cut(s) 65
AvrII CCTAGG 1 cut(s) 118
BbvI GCAGC 2 cut(s) 196, 210
BccI CCATC 1 cut(s) 305
BciT130I CCWGG 1 cut(s) 108
BcoDI GTCTC 3 cut(s) 33, 75, 146
BfaI CTAG 1 cut(s) 119
BfoI RGCGCY 3 cut(s) 59, 86, 150
BisI GCNGC 4 cut(s) 59, 62, 210, 224
BlnI CCTAGG 1 cut(s) 118
BlsI GCNGC 4 cut(s) 60, 63, 211, 225
Bme1390I CCNGG 1 cut(s) 108
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 122
BmrFI CCNGG 1 cut(s) 108
BpmI CTGGAG 1 cut(s) 119
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 2 cut(s) 107, 118
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 233, 305
Bse1I ACTGG 2 cut(s) 175, 189
Bse3DI GCAATG 1 cut(s) 342
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 2 cut(s) 107, 118
BseGI GGATG 1 cut(s) 316
BseLI CCNNNNNNNGG 3 cut(s) 97, 233, 305
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 1 cut(s) 157
BseNI ACTGG 2 cut(s) 175, 189
BseXI GCAGC 2 cut(s) 196, 210
BseYI CCCAGC 1 cut(s) 383
BshFI GGCC 2 cut(s) 77, 123
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 2 cut(s) 74, 91
BslI CCNNNNNNNGG 3 cut(s) 97, 233, 305
BsmAI GTCTC 3 cut(s) 33, 75, 146
BsmBI CGTCTC 1 cut(s) 33
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 2 cut(s) 77, 123
Bso31I GGTCTC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 65
Bsp143I GATC 3 cut(s) 262, 400, 427
BspACI CCGC 3 cut(s) 59, 62, 88
BspANI GGCC 2 cut(s) 77, 123
BspCNI CTCAG 1 cut(s) 156
BspPI GGATC 1 cut(s) 270
BspTNI GGTCTC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 342
BsrI ACTGG 2 cut(s) 175, 189
BssECI CCNNGG 2 cut(s) 107, 118
BssMI GATC 3 cut(s) 262, 400, 427
BssT1I CCWWGG 1 cut(s) 118
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 2 cut(s) 323, 355
BstC8I GCNNGC 3 cut(s) 130, 169, 376
BstDEI CTNAG 1 cut(s) 143
BstF5I GGATG 1 cut(s) 316
BstH2I RGCGCY 3 cut(s) 59, 86, 150
BstHHI GCGC 3 cut(s) 58, 85, 149
BstKTI GATC 3 cut(s) 265, 403, 430
BstMAI GTCTC 3 cut(s) 33, 75, 146
BstMBI GATC 3 cut(s) 262, 400, 427
BstMWI GCNNNNNNNGC 2 cut(s) 129, 380
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 106
BstV1I GCAGC 2 cut(s) 196, 210
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BsuRI GGCC 2 cut(s) 77, 123
BtsCI GGATG 1 cut(s) 316
BtsIMutI CAGTG 1 cut(s) 196
Cac8I GCNNGC 3 cut(s) 130, 169, 376
CfoI GCGC 3 cut(s) 58, 85, 149
Cfr13I GGNCC 1 cut(s) 122
CseI GACGC 1 cut(s) 15
DdeI CTNAG 1 cut(s) 143
DpnI GATC 3 cut(s) 264, 402, 429
DpnII GATC 3 cut(s) 262, 400, 427
EaeI YGGCCR 1 cut(s) 75
EciI GGCGGA 1 cut(s) 77
Eco130I CCWWGG 1 cut(s) 118
Eco31I GGTCTC 1 cut(s) 146
Eco32I GATATC 1 cut(s) 204
Eco47III AGCGCT 1 cut(s) 84
Eco88I CYCGRG 1 cut(s) 65
EcoO109I RGGNCCY 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 106
EcoRV GATATC 1 cut(s) 204
EcoT14I CCWWGG 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 118
Esp3I CGTCTC 1 cut(s) 33
FaiI YATR 2 cut(s) 249, 366
Fnu4HI GCNGC 4 cut(s) 59, 62, 210, 224
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 4 cut(s) 59, 62, 210, 224
FspBI CTAG 1 cut(s) 119
GlaI GCGC 3 cut(s) 57, 84, 148
GluI GCNGC 4 cut(s) 59, 62, 210, 224
GsaI CCCAGC 1 cut(s) 387
GsuI CTGGAG 1 cut(s) 119
HaeII RGCGCY 3 cut(s) 59, 86, 150
HaeIII GGCC 2 cut(s) 77, 123
HapII CCGG 2 cut(s) 74, 91
HgaI GACGC 1 cut(s) 15
HhaI GCGC 3 cut(s) 58, 85, 149
Hin6I GCGC 3 cut(s) 56, 83, 147
HinP1I GCGC 3 cut(s) 56, 83, 147
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 4 cut(s) 50, 68, 94, 388
HpaII CCGG 2 cut(s) 74, 91
HphI GGTGA 2 cut(s) 107, 178
Hpy166II GTNNAC 1 cut(s) 115
Hpy188III TCNNGA 2 cut(s) 98, 346
Hpy8I GTNNAC 1 cut(s) 115
HpyCH4III ACNGT 2 cut(s) 323, 355
HpyCH4V TGCA 1 cut(s) 335
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 380
HpyF3I CTNAG 1 cut(s) 143
HspAI GCGC 3 cut(s) 56, 83, 147
Kzo9I GATC 3 cut(s) 262, 400, 427
LmnI GCTCC 1 cut(s) 90
Lsp1109I GCAGC 2 cut(s) 196, 210
MaeI CTAG 1 cut(s) 119
MaeIII GTNAC 1 cut(s) 323
MalI GATC 3 cut(s) 264, 402, 429
MboI GATC 3 cut(s) 262, 400, 427
MboII GAAGA 1 cut(s) 410
MflI RGATCY 1 cut(s) 427
MluCI AATT 3 cut(s) 276, 294, 417
MlyI GAGTC 1 cut(s) 77
MseI TTAA 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 2 cut(s) 74, 91
MspR9I CCNGG 1 cut(s) 108
Mva1269I GAATGC 1 cut(s) 337
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 129, 380
NdeII GATC 3 cut(s) 262, 400, 427
PctI GAATGC 1 cut(s) 337
PfeI GAWTC 3 cut(s) 50, 94, 388
PkrI GCNGC 4 cut(s) 60, 63, 211, 225
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
Psp6I CCWGG 1 cut(s) 106
PspFI CCCAGC 1 cut(s) 383
PspGI CCWGG 1 cut(s) 106
PspPI GGNCC 1 cut(s) 122
PsuI RGATCY 1 cut(s) 427
PvuII CAGCTG 1 cut(s) 209
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 4 cut(s) 59, 62, 210, 224
Sau3AI GATC 3 cut(s) 262, 400, 427
Sau96I GGNCC 1 cut(s) 122
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 1 cut(s) 108
SetI ASST 8 cut(s) 120, 156, 169, 211, 238, 289, 376, 408
Sse9I AATT 3 cut(s) 276, 294, 417
SsiI CCGC 3 cut(s) 59, 62, 88
SspMI CTAG 1 cut(s) 119
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 2 cut(s) 323, 355
TaqI TCGA 1 cut(s) 200
TasI AATT 3 cut(s) 276, 294, 417
TauI GCSGC 2 cut(s) 61, 64
TfiI GAWTC 3 cut(s) 50, 94, 388
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 1 cut(s) 196
TseI GCWGC 2 cut(s) 209, 223
TspDTI ATGAA 1 cut(s) 353
TspRI CASTG 1 cut(s) 196
XapI RAATTY 1 cut(s) 417
XmaJI CCTAGG 1 cut(s) 118
XspI CTAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.