Rroxscaffold_7G00202580

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0021151
Physical Location & Seq
Forward (+)
50728931 .. 50734395
5465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00202580.1

Sequence Viewer

Length: 381 bp
ATGGTAGCCAAGTCCGATGAAAACCGGCTCTCTTCCTCGATGCACGGGGGTAGGCCTCATCAAAAAGCTCGCGGATCAAGTAGGCTGATGAAGGCCCGCCGGCCTTGGCCTAGTGGTTCTTGCCTAGTTGGGTGTGCTCCCCAACCTAGGTTCGAACCCGAAGCTGTCACGGTGGCCAAACATTGTGCTGCAATGCACATTTGGAGCATTATCAACACCTTCATAGATGATGATGGTCTAACAAGAGGAGAAGAAATATCATCATCACTTCTCAAAGTAATTGAAGAGTCAAGGATTTCGATCATCATATTCTCCGAAAACTATGCATCCTCAAAATGGTGCCTGGATGAACTGGTTCAGATTTTCCACTGCCATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

126

Amino Acids

14.11

Weight (kDa)

7.0

Isoelectric Point (pI)

69.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 71 - 125 9.9e-21 TIR domain
TIR_2 PF13676 71 - 123 9.8e-06 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021151)

Species Orthologous Gene IDs
malus_domestica MD15G1432600.v1.1
rosa_chinensis RchiOBHm_Chr4g0407641
rosa_roxburghii Rroxscaffold_5G00352240 Rroxscaffold_7G00202580
rosa_rugosa Rorug04G0081100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 339
AccII CGCG 1 cut(s) 72
AciI CCGC 2 cut(s) 72, 97
AclWI GGATC 1 cut(s) 82
AcoI YGGCCR 1 cut(s) 174
AfiI CCNNNNNNNGG 2 cut(s) 147, 336
AgsI TTSAA 1 cut(s) 284
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 2 cut(s) 68, 164
AluI AGCT 2 cut(s) 68, 164
Alw21I GWGCWC 1 cut(s) 139
AlwI GGATC 1 cut(s) 82
AoxI GGCC 5 cut(s) 53, 93, 101, 107, 174
ApeKI GCWGC 1 cut(s) 188
Asp700I GAANNNNTTC 1 cut(s) 354
AspA2I CCTAGG 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 153
AvrII CCTAGG 1 cut(s) 146
BalI TGGCCA 1 cut(s) 176
BanI GGYRCC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 1 cut(s) 175
BccI CCATC 1 cut(s) 227
BcgI CGANNNNNNTGC 2 cut(s) 305, 339
BciT130I CCWGG 1 cut(s) 344
BfaI CTAG 3 cut(s) 111, 125, 147
BisI GCNGC 1 cut(s) 189
BlnI CCTAGG 1 cut(s) 146
BlsI GCNGC 1 cut(s) 190
Bme1390I CCNGG 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 1 cut(s) 341
BmrFI CCNGG 1 cut(s) 344
BmsI GCATC 2 cut(s) 30, 335
Bpu14I TTCGAA 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 299
BsaJI CCNNGG 2 cut(s) 104, 146
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 336
Bse118I RCCGGY 2 cut(s) 24, 99
Bse1I ACTGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 198
Bse8I GATNNNNATC 1 cut(s) 299
BseBI CCWGG 1 cut(s) 344
BseDI CCNNGG 2 cut(s) 104, 146
BseGI GGATG 2 cut(s) 326, 352
BseJI GATNNNNATC 1 cut(s) 299
BseLI CCNNNNNNNGG 2 cut(s) 147, 336
BseMI GCAATG 1 cut(s) 198
BseNI ACTGG 1 cut(s) 357
BseRI GAGGAG 1 cut(s) 261
BseXI GCAGC 1 cut(s) 175
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 5 cut(s) 55, 95, 103, 109, 176
BshNI GGYRCC 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 139
BsiSI CCGG 2 cut(s) 25, 100
BslI CCNNNNNNNGG 2 cut(s) 147, 336
BsnI GGCC 5 cut(s) 55, 95, 103, 109, 176
Bsp119I TTCGAA 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 139
Bsp143I GATC 2 cut(s) 74, 300
BspACI CCGC 2 cut(s) 72, 97
BspANI GGCC 5 cut(s) 55, 95, 103, 109, 176
BspFNI CGCG 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 341
BspPI GGATC 1 cut(s) 82
BspT104I TTCGAA 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 339
BsrDI GCAATG 1 cut(s) 198
BsrFI RCCGGY 2 cut(s) 24, 99
BsrI ACTGG 1 cut(s) 357
BssAI RCCGGY 2 cut(s) 24, 99
BssECI CCNNGG 2 cut(s) 104, 146
BssMI GATC 2 cut(s) 74, 300
BssT1I CCWWGG 2 cut(s) 104, 146
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 2 cut(s) 37, 279
BstBI TTCGAA 1 cut(s) 153
BstC8I GCNNGC 3 cut(s) 70, 97, 101
BstF5I GGATG 2 cut(s) 326, 352
BstFNI CGCG 1 cut(s) 72
BstKTI GATC 2 cut(s) 77, 303
BstMBI GATC 2 cut(s) 74, 300
BstNI CCWGG 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 342
BstUI CGCG 1 cut(s) 72
BstV1I GCAGC 1 cut(s) 175
BsuRI GGCC 5 cut(s) 55, 95, 103, 109, 176
BtsCI GGATG 2 cut(s) 326, 352
BtsI GCAGTG 1 cut(s) 367
BtsIMutI CAGTG 1 cut(s) 367
Cac8I GCNNGC 3 cut(s) 70, 97, 101
Cfr10I RCCGGY 2 cut(s) 24, 99
Cfr13I GGNCC 1 cut(s) 94
CviAII CATG 1 cut(s) 374
DpnI GATC 2 cut(s) 76, 302
DpnII GATC 2 cut(s) 74, 300
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 2 cut(s) 37, 279
EarI CTCTTC 2 cut(s) 37, 279
Eco130I CCWWGG 2 cut(s) 104, 146
Eco147I AGGCCT 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 342
EcoT14I CCWWGG 2 cut(s) 104, 146
EcoT22I ATGCAT 1 cut(s) 328
ErhI CCWWGG 2 cut(s) 104, 146
FaeI CATG 1 cut(s) 377
FaiI YATR 4 cut(s) 224, 308, 324, 375
FatI CATG 1 cut(s) 373
FauI CCCGC 1 cut(s) 104
Fnu4HI GCNGC 1 cut(s) 189
FokI GGATG 2 cut(s) 313, 359
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 3 cut(s) 111, 125, 147
GluI GCNGC 1 cut(s) 189
HaeIII GGCC 5 cut(s) 55, 95, 103, 109, 176
HapII CCGG 2 cut(s) 25, 100
Hin1II CATG 1 cut(s) 377
HinfI GANTC 1 cut(s) 287
HpaII CCGG 2 cut(s) 25, 100
Hpy188I TCNGA 3 cut(s) 16, 316, 360
HpyAV CCTTC 2 cut(s) 85, 229
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4V TGCA 4 cut(s) 43, 191, 196, 326
Hsp92II CATG 1 cut(s) 377
KroI GCCGGC 1 cut(s) 99
KroNI GCCGGC 1 cut(s) 101
Kzo9I GATC 2 cut(s) 74, 300
LmnI GCTCC 2 cut(s) 142, 204
LpnPI CCDG 5 cut(s) 38, 113, 329, 338, 356
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 2 cut(s) 30, 335
MaeI CTAG 3 cut(s) 111, 125, 147
MaeIII GTNAC 1 cut(s) 166
MalI GATC 2 cut(s) 76, 302
MboI GATC 2 cut(s) 74, 300
MboII GAAGA 3 cut(s) 24, 263, 296
MhlI GDGCHC 1 cut(s) 139
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 1 cut(s) 279
MluNI TGGCCA 1 cut(s) 176
MlyI GAGTC 1 cut(s) 296
MnlI CCTC 4 cut(s) 46, 66, 239, 340
Mox20I TGGCCA 1 cut(s) 176
Mph1103I ATGCAT 1 cut(s) 328
MroNI GCCGGC 1 cut(s) 99
MroXI GAANNNNTTC 1 cut(s) 354
MscI TGGCCA 1 cut(s) 176
MslI CAYNNNNRTG 1 cut(s) 372
Msp20I TGGCCA 1 cut(s) 176
MspI CCGG 2 cut(s) 25, 100
MspR9I CCNGG 1 cut(s) 344
MvaI CCWGG 1 cut(s) 344
MvnI CGCG 1 cut(s) 72
NaeI GCCGGC 1 cut(s) 101
NdeII GATC 2 cut(s) 74, 300
NgoMIV GCCGGC 1 cut(s) 99
NlaIII CATG 1 cut(s) 377
NlaIV GGNNCC 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 166
NsiI ATGCAT 1 cut(s) 328
NspV TTCGAA 1 cut(s) 153
PceI AGGCCT 1 cut(s) 55
PdiI GCCGGC 1 cut(s) 101
PdmI GAANNNNTTC 1 cut(s) 354
PkrI GCNGC 1 cut(s) 190
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Psp6I CCWGG 1 cut(s) 342
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 341
PspPI GGNCC 1 cut(s) 94
RseI CAYNNNNRTG 1 cut(s) 372
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 2 cut(s) 74, 300
Sau96I GGNCC 1 cut(s) 94
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 1 cut(s) 344
SduI GDGCHC 1 cut(s) 139
SetI ASST 5 cut(s) 70, 148, 152, 166, 221
SfaNI GCATC 2 cut(s) 30, 335
SfuI TTCGAA 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 372
Sse9I AATT 1 cut(s) 279
SseBI AGGCCT 1 cut(s) 55
SsiI CCGC 2 cut(s) 72, 97
SspMI CTAG 3 cut(s) 111, 125, 147
StuI AGGCCT 1 cut(s) 55
StyD4I CCNGG 1 cut(s) 342
StyI CCWWGG 2 cut(s) 104, 146
TaaI ACNGT 1 cut(s) 172
TaqI TCGA 3 cut(s) 38, 153, 299
TasI AATT 1 cut(s) 279
TscAI CASTG 1 cut(s) 374
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 1 cut(s) 188
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 4 cut(s) 33, 104, 211, 363
TspRI CASTG 1 cut(s) 374
XmaJI CCTAGG 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 3 cut(s) 111, 125, 147
Zsp2I ATGCAT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.