Rorug01G0031700

RING-box protein 1a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0017888
Physical Location & Seq
Forward (+)
5364844 .. 5369787
4944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0031700.1

Sequence Viewer

Length: 4326 bp
ATGCCTATTGTTCGAGCACTTGAATCTGCTTCTGTAAGCATCCTCCTTAACAAGTTGGCCACTCAAGATGTCATAGATTTCTTCCTCAAATGGAAAATTGATGATTCCCTGCGAGAAAAGCTCAAGAGAAACCTGCTTGTCATTCATGCAGTTCTCAATCATGCTGAGGAGAAGCAAGTCCGGGACTCAAATGTCAAAGCTTGGCTCGAGGACGTCAGAGTTGCAGCTTATGATGCCGAGGACATATTGGATGACATCGCCAATGATGCTCTGGAATCACAAAACAGCAAACATGAGGTATGGAATTCTAAGATCAAAGAAGGGATAGACTTTAAGATCAAAGACATTGCTAATACTCTTAATCCTTTTAGAGAACGTATAGAGTCGAAAATGATAAAGATTATAGATGTATTGGAAGAGATTGCAAAGCAGAAAGATATTCTTCGTTTGAGAGAAGATTCTGGCGGTATAGAATCAGGTTTTGACAGATTGCCCACGACTCCAATGTTGGGTGATGAATCTCATGTTTATGGTAGAGAATCTGATAAGGAGGAGATAATTACTTTGCTGAATTTGGGTGGAAATGATGATGGGGTTTCTATAATCCCAATTGTAGGCATGGGAGGTATTGGGAAGACTACACTTGCTCAGCTTGTGTATAATGATGCTAATGTGAGTAGCCATTTTGATTTGAGAGCCTGGGCTTGTGTCTCTGATGTATTTGATGTGCAGAGGATAACAAAAACACTTGTTGAGTCTGCGACTAAATCAAGTTGTAGCACAAACAATTTGGAGTTACTTCAAGAACAACTGAAAATACGGTTGAAGAACAAGAAATTTCTTTTTGTTCTGGATGATGTCTGGAACGAGAAGTATGAAAGCTGGGATAAGTTAAGAGTCCCCTTCACAGTTGGTGCACCAGGGAGTAAAATCATAATCACGACACGAAACAAAACAGTTGCATCACTTATGCGGACTGTTCCTACTTACTGTTTGGAGGGGCTTTCGGACAGTGATTGTTGGTTATTATTCGAACAAATTGTGTTTCAGAACAGGAATTTAGATGCCTATCCAAAGTTGAAAGTGATTGGCAAGAAAATAGCATACAAGTGTAAAGGGTTGCCTTTGGCTGCAAAGGCACTAGGAGGTCTCCTGCGTGCTGAACCGGAACTAGATGAGAATTACTGGAATGATATCTTGAATAGCAAACTTTGGAAACTGGCAGACAACACTATTCTTCCAGCTCTAAGATTAAGCTACCATCACCTCCCTGGGCATTTGAAGCGCTGCTTTGCTTATTGCTCGATGTTTCCTAAGGACTATGAATATGAGAGAGAGATGTTAATCTTGTTATGGATGGCAGAGGGTTTTGTGCCAGAGCCAGAAGGAAATCAACGGATCGAAGATGTAGCTGGAAGATATGTTTCAGATTTTTTATCAAGGTCCTTCTTCCAACACACCAGTGACAAGTCACGACTGGTAATGCATGACCTCATACATGATCTTGCACAATCTGTTTCTGGCAAAACATTTGTTAGGTTGGAGGAAAATTCAGAGAACCATAAAAAGTCTACAAAGGCTCGGCACTTGTCATATACCCGTGGATCTGAAGATGTATTTCAGAAATTTGAGGCATTCAGTGAGGTGGATTGTTTACGAACCTTTCTACCACTAGACCCGTTGCAAGGATTCAATGTGAGTGGCTTATCTGACAAGGTTCCTTGTGATATGATGCCAAAATTAAGACTCCTGCGGGTGCTATCCTTTAGTGGTTATCTCATAGAAAAGTTGCCAGATTCAATTGGCAACTTGAAGCATCTGCGATACCTCAATCTTTCTTATTGTCAAATAGAAGAGCTACCTGAGCCAACAAGCAGTCTATACAATCTGCAGACATTAATTTTATTCAGATGTCGATCGCTTACCATGCTGCCTGCAGACATAGGAAACCTAAGGAATTTACGGCATCTCAATATTCAGGGAACTAGGCTGAAAAGTATGCCTTTGGGAATGGGAAGATTGGTTAACCTTCAAATACTGTCAGATTTTATTGTGGGGAAAGCCTTGGGAAAAGGGATTGCAGAATTGAAGGACTTGTCACATCTTCGAGGCTCAGTTTCTATTTTTTGTTTGCATGATGTCAGCAGTGTTAGGGATGCAATTGAGGCCAGATTAGAGGTTAAGAAGCACCTTGATGATTTAGTTTTGGAATGGAGCAGCAGTACTGATGGTTCGCGAAATGAAGAGATCGAGACAGAGGTACTTGATGCACTGCAGCCTCATGAAAACCTGAAGAAACTCACCATTAAGTATTATGGAGGTACAGAATTTCCATATTGGATGGGGAATCCTTTGTTCACTAACATGGCGTACTTGCATCTCTATGGTTGCATAAAATGCACATCTTTACCACCCCTTGGCCAACTACCTTTTCTTAAAGATCTCATAATTGAAGCAATGCATGGAATACAGCGTGTAGGAATGGATTTTTTTGGGGATGATGATGGTTCCACAATTTTTTTCCCCTCTTTAGAGACTTTGAAATTTGAGGACATGAAAGAATGGGAGAAATGGTCATCTTCAGAAGATGAAAAGATTAAAGGATTTCCCAGCCTTCGGGAGCTTTCCATATTTAGATGTCCCAAGTTAACAAAGTTTACACGTGGATTTGCTTCTCTAGAAAAGCTGCTCATCAAGAACTGCGGAGCAATAACTACCTTCTCACAGAATCCTGCACTTGGGAATTTGGAACCTGTAGAATTCCCTAGCCTGAATTTGGAACCTGTAGAATTCCCTAGCCTGCAACTGCTTGTTCTTGTTGGTTGCAGTGAGCTTGAAAACCTTCCTTTTTCCCTCCCTTTGTTGAAAATGCTTGACATAGATGGTTGTGAAAAATTGGCCGCACTTCCAAGGATGGTGGAACTTAGTACATTGTACCTGATCGATTCAAATGCTGAATTACTTGGATCCAAGATGGAATTTAGCTCGCTGACCTCTTTGCACCTAACTCATATTCCAGATGTGAAGCATCTACCTGAAGGCTTTATCCAACAGTCTGCAAAACTTGAAAAATTGAGTCTTACAAGCTTTCCTGACCTTGAATATTTGGCAGATGACGGAGTTGGACTAGCACACCTTGTATCCCTTCAACGCTTGACAATATCCCATTGTCCTAAGTTTGTTGCTTTGCCTGATGAGCTCCCACCTGTACTTAAATATTTGGATTTGAAACATTGTCCTAGCCTGGTGAAGCTGCCTGGTAAACTTCACAACCTTGAGTGTCTTGCAGAGCTGCAGATTGAATGGTGTTCAAAGATTGAAGCATTTCCAGATGTGGGGTTGCCTGACAAACTGAAGCGTCTTGTAATCCGTGAATGTGGTGCTCTGAAGACCCTTCCTCAGGAGCTTCTCTGCAATAACAATTTTCTCGAGTATTTAGAAGTTCATAAATGTCCTTCTCTTGTGTCAGTTCTTGAAGAAGGAAACTTGCCATCAACTCTAACACATATGAAATTTTACTATTGCAAAATTCTGGGATCACTGCCTGAGGGTTTGATGCACAAAGACAATATGACTCTAAAGTACTTGGAGATAGACAACTGTTCCTCTCTCATGTCCTTTCCAAATGGTGAGTTACCAACAGGCCTGGAGCGTCTTGAGATTAGTGATTGCTCTAACTTCCAGACATTGCCATTGAGCCTTCTTAACCTCACGAATCTTGAGACCTTGGAAGTAAAGAGCTGCCCCCTTCTTCAGGACTTTCCAGAGGGTGGATTGCCACCCAACATAAAGTTTGTCACAATTTCCGAATGTGAGAATCTCAAGTCTCTACCTAAATTCTTTCATAAACTCAAATGTCTTCAGAAGTTGGAGATTTCTGGCTGTCCTAGTCTCCTGTCCTTGCCAAAGCAGGGTCTGCCCAGCAGCTTAAGACTACTTACAGTTACTGATTGTGAAAAGCTTAATCCCATACATCAGTGGAGACTTCACAAACTACCCTCTCTAGATGTTTTCACCATTGGTGGATTTCCTGGCCTGAAGTCCTTTTCAAAAGATTATCCTCTTCCTCACAGTTTAACCTCTTTAAGCATTCAAAGATTCCCAGATCTTGAATCCATATCGGAGGTGCTTGAGAATCTCACCTCACTTGAAAACCTGAGTATCAGGGAGTGTGATAAGCTCCAATCTTTGCCAGAGACGGGGCTACCAGCGACAATTTCTACCTTGACAATTCGGAATTGTTTACTTCTAGAACCTCGATGTGAAAAGGACAAAGGAGAAGATTGGTCCAAGATCAAAAACATCCCCTTCGTGAGTTCACGCTAA

Protein Analysis

1441

Amino Acids

162.87

Weight (kDa)

5.75

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 98 1.4e-16 Rx N-terminal domain
NB-ARC PF00931 180 - 352 6.2e-37 NB-ARC domain
WHD_DRP PF23559 436 - 503 1.1e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 578 - 852 2.6e-30 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 693 - 820 6.9e-40 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017888)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G42830
rosa_chinensis RchiOBHm_Chr1g0321161
rosa_laevigata RLG00000030435
rosa_multiflora Rmu_co8330811.1_g000001 Rmu_sc0004660.1_g000010
rosa_rugosa Rorug01G0031700
rosa_samantha Rh1AG043500 Rh1BG040700 Rh1DG050600
rosa_wichuraiana Rw1G003810 Rw2G038860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AatII GACGTC 1 cut(s) 216
Acc36I ACCTGC 1 cut(s) 141
AccB7I CCANNNNNTGG 2 cut(s) 2417, 3770
AccI GTMKAC 1 cut(s) 1571
AccII CGCG 1 cut(s) 2236
AciI CCGC 5 cut(s) 465, 973, 1753, 2703, 2901
AclWI GGATC 5 cut(s) 1406, 1612, 2961, 2974, 3544
AcoI YGGCCR 3 cut(s) 57, 2419, 2898
AcuI CTGAAG 9 cut(s) 1629, 2312, 2565, 3057, 3374, 3407, 3737, 3845, 4058
AcvI CACGTG 1 cut(s) 2663
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 8 cut(s) 2224, 2262, 2323, 2372, 2929, 2936, 3210, 3584
AfeI AGCGCT 1 cut(s) 1286
AflII CTTAAG 1 cut(s) 3928
AflIII ACRYGT 1 cut(s) 2660
AjnI CCWGG 7 cut(s) 698, 919, 1270, 3243, 3256, 3645, 4030
AjuI GAANNNNNNNTTGG 2 cut(s) 2415, 2447
AleI CACNNNNGTG 1 cut(s) 1461
Alw21I GWGCWC 4 cut(s) 19, 919, 3201, 3385
Alw26I GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3716, 3831, 3896, 3976, 4190
Alw44I GTGCAC 1 cut(s) 915
AlwI GGATC 5 cut(s) 1406, 1612, 2961, 2974, 3544
AlwNI CAGNNNCTG 2 cut(s) 3916, 3947
Ama87I CYCGRG 2 cut(s) 206, 3428
Aor51HI AGCGCT 1 cut(s) 1286
AoxI GGCC 6 cut(s) 57, 2166, 2419, 2898, 3643, 4033
ApaLI GTGCAC 1 cut(s) 915
AseI ATTAAT 1 cut(s) 1898
Asp700I GAANNNNTTC 2 cut(s) 2841, 3324
AspLEI GCGC 1 cut(s) 1287
AspS9I GGNCC 2 cut(s) 1443, 4287
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 7 cut(s) 524, 1256, 2293, 3259, 3641, 4006, 4132
AsuII TTCGAA 1 cut(s) 1032
AvaI CYCGRG 2 cut(s) 206, 3428
AvaII GGWCC 2 cut(s) 1443, 4287
AxyI CCTNAGG 4 cut(s) 1314, 1952, 3399, 3546
BaeGI GKGCMC 1 cut(s) 919
BaeI ACNNNNGTAYC 2 cut(s) 4144, 4177
BalI TGGCCA 2 cut(s) 59, 2421
BamHI GGATCC 1 cut(s) 2966
BanII GRGCYC 1 cut(s) 3201
BbrPI CACGTG 1 cut(s) 2663
BbsI GAAGAC 3 cut(s) 641, 3395, 3851
Bbv12I GWGCWC 4 cut(s) 19, 919, 3201, 3385
BbvCI CCTCAGC 1 cut(s) 165
BceAI ACGGC 1 cut(s) 1979
BcgI CGANNNNNNTGC 2 cut(s) 2933, 2967
BciT130I CCWGG 7 cut(s) 700, 921, 1272, 3245, 3258, 3647, 4032
BciVI GTATCC 1 cut(s) 3151
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3716, 3831, 3896, 3976, 4190
BfmI CTRYAG 6 cut(s) 1889, 1935, 2273, 2754, 2784, 3293
BfoI RGCGCY 1 cut(s) 1288
BfrI CTTAAG 1 cut(s) 3928
BfuAI ACCTGC 1 cut(s) 141
BfuI GTATCC 1 cut(s) 3151
BglII AGATCT 2 cut(s) 2440, 4105
BlpI GCTNAGC 1 cut(s) 648
BmcAI AGTACT 2 cut(s) 2224, 3584
Bme1390I CCNGG 8 cut(s) 182, 700, 921, 1272, 3245, 3258, 3647, 4032
Bme18I GGWCC 2 cut(s) 1443, 4287
BmeT110I CYCGRG 2 cut(s) 206, 3428
BmgT120I GGNCC 2 cut(s) 1443, 4287
BmiI GGNNCC 5 cut(s) 1719, 2509, 2751, 2781, 2968
BmrFI CCNGG 8 cut(s) 182, 700, 921, 1272, 3245, 3258, 3647, 4032
BpiI GAAGAC 3 cut(s) 641, 3395, 3851
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 1 cut(s) 3668
Bpu10I CCTNAGC 2 cut(s) 165, 1863
Bpu1102I GCTNAGC 1 cut(s) 648
Bpu14I TTCGAA 1 cut(s) 1032
BpuEI CTTGAG 7 cut(s) 48, 107, 3296, 3677, 3740, 3806, 4151
BpuMI CCSGG 1 cut(s) 182
Bsa29I ATCGAT 1 cut(s) 2943
BsaAI YACGTR 1 cut(s) 2663
BsaBI GATNNNNATC 3 cut(s) 1068, 1343, 1915
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 2 cut(s) 1154, 3716
BsaWI WCCGGW 1 cut(s) 1165
BsaXI ACNNNNNCTCC 4 cut(s) 2206, 2236, 2558, 2588
Bse1I ACTGG 4 cut(s) 1190, 1224, 1461, 1482
Bse21I CCTNAGG 4 cut(s) 1314, 1952, 3399, 3546
Bse3DI GCAATG 3 cut(s) 345, 2463, 3686
Bse8I GATNNNNATC 3 cut(s) 1068, 1343, 1915
BseBI CCWGG 7 cut(s) 700, 921, 1272, 3245, 3258, 3647, 4032
BseCI ATCGAT 1 cut(s) 2943
BseGI GGATG 9 cut(s) 39, 256, 859, 1362, 2161, 2346, 2503, 2919, 4302
BseJI GATNNNNATC 3 cut(s) 1068, 1343, 1915
BseMI GCAATG 3 cut(s) 345, 2463, 3686
BseMII CTCAG 7 cut(s) 156, 662, 1854, 2127, 3413, 3537, 4148
BseNI ACTGG 4 cut(s) 1190, 1224, 1461, 1482
BseRI GAGGAG 2 cut(s) 182, 566
BseSI GKGCMC 1 cut(s) 919
BseYI CCCAGC 3 cut(s) 882, 2609, 3920
BsgI GTGCAG 2 cut(s) 749, 2718
Bsh1236I CGCG 1 cut(s) 2236
Bsh1285I CGRYCG 1 cut(s) 1919
BshFI GGCC 6 cut(s) 59, 2168, 2421, 2900, 3645, 4035
BshVI ATCGAT 1 cut(s) 2943
BsiEI CGRYCG 1 cut(s) 1919
BsiHKAI GWGCWC 4 cut(s) 19, 919, 3201, 3385
BsiHKCI CYCGRG 2 cut(s) 206, 3428
BsiSI CCGG 2 cut(s) 181, 1166
BslFI GGGAC 3 cut(s) 197, 884, 2625
BsmAI GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3716, 3831, 3896, 3976, 4190
BsmBI CGTCTC 1 cut(s) 4190
BsmFI GGGAC 3 cut(s) 197, 884, 2625
BsmI GAATGC 2 cut(s) 1634, 4089
BsnI GGCC 6 cut(s) 59, 2168, 2421, 2900, 3645, 4035
Bso31I GGTCTC 2 cut(s) 1154, 3716
BsoBI CYCGRG 2 cut(s) 206, 3428
Bsp119I TTCGAA 1 cut(s) 1032
Bsp1286I GDGCHC 4 cut(s) 19, 919, 3201, 3385
Bsp1720I GCTNAGC 1 cut(s) 648
Bsp68I TCGCGA 1 cut(s) 2236
BspACI CCGC 5 cut(s) 465, 973, 1753, 2703, 2901
BspANI GGCC 6 cut(s) 59, 2168, 2421, 2900, 3645, 4035
BspCNI CTCAG 7 cut(s) 157, 661, 1855, 2126, 3412, 3538, 4149
BspDI ATCGAT 1 cut(s) 2943
BspFNI CGCG 1 cut(s) 2236
BspHI TCATGA 1 cut(s) 2281
BspLI GGNNCC 5 cut(s) 1719, 2509, 2751, 2781, 2968
BspMAI CTGCAG 4 cut(s) 1893, 1939, 2277, 3297
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 5 cut(s) 1406, 1612, 2961, 2974, 3544
BspQI GCTCTTC 1 cut(s) 1848
BspT104I TTCGAA 1 cut(s) 1032
BspTI CTTAAG 1 cut(s) 3928
BspTNI GGTCTC 2 cut(s) 1154, 3716
BsrDI GCAATG 3 cut(s) 345, 2463, 3686
BsrI ACTGG 4 cut(s) 1190, 1224, 1461, 1482
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 4 cut(s) 2064, 2416, 2909, 3726
Bst2UI CCWGG 7 cut(s) 700, 921, 1272, 3245, 3258, 3647, 4032
Bst6I CTCTTC 4 cut(s) 411, 1848, 2238, 4068
BstACI GRCGYC 1 cut(s) 213
BstAFI CTTAAG 1 cut(s) 3928
BstAPI GCANNNNNTGC 2 cut(s) 2397, 3916
BstBAI YACGTR 1 cut(s) 2663
BstBI TTCGAA 1 cut(s) 1032
BstC8I GCNNGC 4 cut(s) 1158, 1935, 2801, 2987
BstDSI CCRYGG 1 cut(s) 1600
BstENI CCTNNNNNAGG 2 cut(s) 3398, 3752
BstF5I GGATG 9 cut(s) 39, 256, 859, 1362, 2161, 2346, 2503, 2919, 4302
BstFNI CGCG 1 cut(s) 2236
BstH2I RGCGCY 1 cut(s) 1288
BstHHI GCGC 1 cut(s) 1287
BstMAI GTCTC 9 cut(s) 715, 1154, 2246, 2528, 3716, 3831, 3896, 3976, 4190
BstMCI CGRYCG 1 cut(s) 1919
BstNI CCWGG 7 cut(s) 700, 921, 1272, 3245, 3258, 3647, 4032
BstSCI CCNGG 8 cut(s) 180, 698, 919, 1270, 3243, 3256, 3645, 4030
BstSFI CTRYAG 6 cut(s) 1889, 1935, 2273, 2754, 2784, 3293
BstSLI GKGCMC 1 cut(s) 919
BstUI CGCG 1 cut(s) 2236
BstV2I GAAGAC 3 cut(s) 641, 3395, 3851
BstX2I RGATCY 4 cut(s) 1604, 2440, 2966, 4105
BstYI RGATCY 4 cut(s) 1604, 2440, 2966, 4105
Bsu15I ATCGAT 1 cut(s) 2943
Bsu36I CCTNAGG 4 cut(s) 1314, 1952, 3399, 3546
BsuI GTATCC 1 cut(s) 3151
BsuRI GGCC 6 cut(s) 59, 2168, 2421, 2900, 3645, 4035
BsuTUI ATCGAT 1 cut(s) 2943
BtgI CCRYGG 1 cut(s) 1600
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 9 cut(s) 39, 256, 859, 1362, 2161, 2346, 2503, 2919, 4302
BtsI GCAGTG 4 cut(s) 2152, 2270, 2833, 3539
BtsIMutI CAGTG 8 cut(s) 1018, 1468, 1645, 2152, 2270, 2833, 3539, 3983
BtuMI TCGCGA 1 cut(s) 2236
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 4 cut(s) 1158, 1935, 2801, 2987
CaiI CAGNNNCTG 2 cut(s) 3916, 3947
CciI TCATGA 1 cut(s) 2281
CfoI GCGC 1 cut(s) 1287
Cfr13I GGNCC 2 cut(s) 1443, 4287
ClaI ATCGAT 1 cut(s) 2943
CseI GACGC 2 cut(s) 3347, 3641
Csp6I GTAC 8 cut(s) 2223, 2261, 2322, 2371, 2928, 2935, 3209, 3583
CspCI CAANNNNNGTGG 4 cut(s) 1681, 1716, 2898, 2933
CviQI GTAC 8 cut(s) 2223, 2261, 2322, 2371, 2928, 2935, 3209, 3583
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 3 cut(s) 57, 2419, 2898
Eam1104I CTCTTC 4 cut(s) 411, 1848, 2238, 4068
EarI CTCTTC 4 cut(s) 411, 1848, 2238, 4068
Ecl136II GAGCTC 1 cut(s) 3199
Eco130I CCWWGG 4 cut(s) 2064, 2416, 2909, 3726
Eco147I AGGCCT 1 cut(s) 3645
Eco24I GRGCYC 1 cut(s) 3201
Eco31I GGTCTC 2 cut(s) 1154, 3716
Eco32I GATATC 1 cut(s) 1195
Eco47I GGWCC 2 cut(s) 1443, 4287
Eco47III AGCGCT 1 cut(s) 1286
Eco53kI GAGCTC 1 cut(s) 3199
Eco57I CTGAAG 9 cut(s) 1629, 2312, 2565, 3057, 3374, 3407, 3737, 3845, 4058
Eco72I CACGTG 1 cut(s) 2663
Eco81I CCTNAGG 4 cut(s) 1314, 1952, 3399, 3546
Eco88I CYCGRG 2 cut(s) 206, 3428
EcoICRI GAGCTC 1 cut(s) 3199
EcoNI CCTNNNNNAGG 2 cut(s) 3398, 3752
EcoO109I RGGNCCY 1 cut(s) 1443
EcoRI GAATTC 3 cut(s) 304, 2759, 2789
EcoRII CCWGG 7 cut(s) 698, 919, 1270, 3243, 3256, 3645, 4030
EcoRV GATATC 1 cut(s) 1195
EcoT14I CCWWGG 4 cut(s) 2064, 2416, 2909, 3726
EcoT22I ATGCAT 2 cut(s) 1488, 2463
EcoT38I GRGCYC 1 cut(s) 3201
ErhI CCWWGG 4 cut(s) 2064, 2416, 2909, 3726
Esp3I CGTCTC 1 cut(s) 4190
FalI AAGNNNNNCTT 6 cut(s) 426, 458, 1274, 1306, 1987, 2019
FaqI GGGAC 3 cut(s) 197, 884, 2625
FauI CCCGC 1 cut(s) 1746
FauNDI CATATG 1 cut(s) 3507
FblI GTMKAC 1 cut(s) 1571
FokI GGATG 9 cut(s) 26, 263, 866, 1369, 2168, 2353, 2510, 2926, 4289
FriOI GRGCYC 1 cut(s) 3201
GlaI GCGC 1 cut(s) 1286
GsaI CCCAGC 3 cut(s) 886, 2613, 3924
GsuI CTGGAG 1 cut(s) 3668
HaeII RGCGCY 1 cut(s) 1288
HaeIII GGCC 6 cut(s) 59, 2168, 2421, 2900, 3645, 4035
HapII CCGG 2 cut(s) 181, 1166
HgaI GACGC 2 cut(s) 3347, 3641
HhaI GCGC 1 cut(s) 1287
Hin1I GRCGYC 1 cut(s) 213
Hin6I GCGC 1 cut(s) 1285
HinP1I GCGC 1 cut(s) 1285
HincII GTYRAC 2 cut(s) 2026, 2649
HindII GTYRAC 2 cut(s) 2026, 2649
HindIII AAGCTT 3 cut(s) 198, 3085, 3959
HpaI GTTAAC 2 cut(s) 2026, 2649
HpaII CCGG 2 cut(s) 181, 1166
HphI GGTGA 7 cut(s) 524, 1256, 2293, 3259, 3641, 4006, 4132
HpyCH4IV ACGT 3 cut(s) 213, 376, 2662
HpySE526I ACGT 3 cut(s) 213, 376, 2662
Hsp92I GRCGYC 1 cut(s) 213
HspAI GCGC 1 cut(s) 1285
KspAI GTTAAC 2 cut(s) 2026, 2649
LguI GCTCTTC 1 cut(s) 1848
LmnI GCTCC 7 cut(s) 2214, 2620, 2705, 3204, 3403, 3649, 4185
MaeII ACGT 3 cut(s) 213, 376, 2662
MaeIII GTNAC 7 cut(s) 795, 1463, 1470, 2097, 3633, 3796, 3943
MfeI CAATTG 3 cut(s) 609, 1800, 2160
MflI RGATCY 4 cut(s) 1604, 2440, 2966, 4105
MhlI GDGCHC 4 cut(s) 19, 919, 3201, 3385
MlsI TGGCCA 2 cut(s) 59, 2421
MluNI TGGCCA 2 cut(s) 59, 2421
MlyI GAGTC 8 cut(s) 179, 392, 493, 764, 906, 1740, 3085, 3568
MmeI TCCRAC 5 cut(s) 1477, 1521, 3073, 3103, 3849
Mox20I TGGCCA 2 cut(s) 59, 2421
Mph1103I ATGCAT 2 cut(s) 1488, 2463
MroXI GAANNNNTTC 2 cut(s) 2841, 3324
MscI TGGCCA 2 cut(s) 59, 2421
MslI CAYNNNNRTG 6 cut(s) 528, 1108, 1461, 2193, 2363, 2382
Msp20I TGGCCA 2 cut(s) 59, 2421
MspCI CTTAAG 1 cut(s) 3928
MspI CCGG 2 cut(s) 181, 1166
MspR9I CCNGG 8 cut(s) 182, 700, 921, 1272, 3245, 3258, 3647, 4032
MunI CAATTG 3 cut(s) 609, 1800, 2160
Mva1269I GAATGC 2 cut(s) 1634, 4089
MvaI CCWGG 7 cut(s) 700, 921, 1272, 3245, 3258, 3647, 4032
MvnI CGCG 1 cut(s) 2236
NciI CCSGG 1 cut(s) 182
NdeI CATATG 1 cut(s) 3507
NlaIV GGNNCC 5 cut(s) 1719, 2509, 2751, 2781, 2968
NmeAIII GCCGAG 2 cut(s) 262, 1561
NmuCI GTSAC 4 cut(s) 1463, 1470, 2097, 3796
NruI TCGCGA 1 cut(s) 2236
NsiI ATGCAT 2 cut(s) 1488, 2463
NspV TTCGAA 1 cut(s) 1032
OliI CACNNNNGTG 1 cut(s) 1461
PaeR7I CTCGAG 2 cut(s) 206, 3428
PagI TCATGA 1 cut(s) 2281
PasI CCCWGGG 1 cut(s) 1271
PceI AGGCCT 1 cut(s) 3645
PciSI GCTCTTC 1 cut(s) 1848
PctI GAATGC 2 cut(s) 1634, 4089
PdmI GAANNNNTTC 2 cut(s) 2841, 3324
PflMI CCANNNNNTGG 2 cut(s) 2417, 3770
PfoI TCCNGGA 1 cut(s) 180
Ple19I CGATCG 1 cut(s) 1919
PleI GAGTC 8 cut(s) 179, 391, 493, 763, 905, 1740, 3084, 3568
PmaCI CACGTG 1 cut(s) 2663
PmlI CACGTG 1 cut(s) 2663
PpsI GAGTC 8 cut(s) 179, 391, 493, 763, 905, 1740, 3084, 3568
Ppu21I YACGTR 1 cut(s) 2663
PpuMI RGGWCCY 1 cut(s) 1443
PshBI ATTAAT 1 cut(s) 1898
Psp124BI GAGCTC 1 cut(s) 3201
Psp5II RGGWCCY 1 cut(s) 1443
Psp6I CCWGG 7 cut(s) 698, 919, 1270, 3243, 3256, 3645, 4030
PspCI CACGTG 1 cut(s) 2663
PspFI CCCAGC 3 cut(s) 882, 2609, 3920
PspGI CCWGG 7 cut(s) 698, 919, 1270, 3243, 3256, 3645, 4030
PspN4I GGNNCC 5 cut(s) 1719, 2509, 2751, 2781, 2968
PspPI GGNCC 2 cut(s) 1443, 4287
PspPPI RGGWCCY 1 cut(s) 1443
PspXI VCTCGAGB 1 cut(s) 206
PsrI GAACNNNNNNTAC 2 cut(s) 2215, 2247
PstI CTGCAG 4 cut(s) 1893, 1939, 2277, 3297
PstNI CAGNNNCTG 2 cut(s) 3916, 3947
PsuI RGATCY 4 cut(s) 1604, 2440, 2966, 4105
PvuI CGATCG 1 cut(s) 1919
RruI TCGCGA 1 cut(s) 2236
RsaI GTAC 8 cut(s) 2224, 2262, 2323, 2372, 2929, 2936, 3210, 3584
RsaNI GTAC 8 cut(s) 2223, 2261, 2322, 2371, 2928, 2935, 3209, 3583
RseI CAYNNNNRTG 6 cut(s) 528, 1108, 1461, 2193, 2363, 2382
SacI GAGCTC 1 cut(s) 3201
SapI GCTCTTC 1 cut(s) 1848
Sau96I GGNCC 2 cut(s) 1443, 4287
ScaI AGTACT 2 cut(s) 2224, 3584
SchI GAGTC 8 cut(s) 179, 392, 493, 764, 906, 1740, 3085, 3568
ScrFI CCNGG 8 cut(s) 182, 700, 921, 1272, 3245, 3258, 3647, 4032
SduI GDGCHC 4 cut(s) 19, 919, 3201, 3385
SfcI CTRYAG 6 cut(s) 1889, 1935, 2273, 2754, 2784, 3293
Sfr274I CTCGAG 2 cut(s) 206, 3428
SfuI TTCGAA 1 cut(s) 1032
SinI GGWCC 2 cut(s) 1443, 4287
SlaI CTCGAG 2 cut(s) 206, 3428
SmiMI CAYNNNNRTG 6 cut(s) 528, 1108, 1461, 2193, 2363, 2382
SseBI AGGCCT 1 cut(s) 3645
SsiI CCGC 5 cut(s) 465, 973, 1753, 2703, 2901
SspI AATATT 3 cut(s) 1975, 3104, 3218
SstI GAGCTC 1 cut(s) 3201
StuI AGGCCT 1 cut(s) 3645
StyD4I CCNGG 8 cut(s) 180, 698, 919, 1270, 3243, 3256, 3645, 4030
StyI CCWWGG 4 cut(s) 2064, 2416, 2909, 3726
TaiI ACGT 3 cut(s) 216, 379, 2665
TatI WGTACW 4 cut(s) 2222, 2927, 3208, 3582
TauI GCSGC 1 cut(s) 2903
TscAI CASTG 8 cut(s) 1018, 1468, 1645, 2152, 2277, 2833, 3546, 3983
TseFI GTSAC 4 cut(s) 1463, 1470, 2097, 3796
Tsp45I GTSAC 4 cut(s) 1463, 1470, 2097, 3796
TspGWI ACGGA 3 cut(s) 1411, 3132, 3359
TspRI CASTG 8 cut(s) 1018, 1468, 1645, 2152, 2277, 2833, 3546, 3983
Van91I CCANNNNNTGG 2 cut(s) 2417, 3770
Vha464I CTTAAG 1 cut(s) 3928
VneI GTGCAC 1 cut(s) 915
VpaK11BI GGWCC 2 cut(s) 1443, 4287
VspI ATTAAT 1 cut(s) 1898
XagI CCTNNNNNAGG 2 cut(s) 3398, 3752
XbaI TCTAGA 3 cut(s) 2677, 4003, 4249
XcmI CCANNNNNNNNNTGG 2 cut(s) 268, 1268
XhoI CTCGAG 2 cut(s) 206, 3428
XmiI GTMKAC 1 cut(s) 1571
XmnI GAANNNNTTC 2 cut(s) 2841, 3324
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 2 cut(s) 2224, 3584
Zsp2I ATGCAT 2 cut(s) 1488, 2463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.