Rorug01G0194300

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0032265
Physical Location & Seq
Forward (+)
27086557 .. 27087641
1085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0194300.1

Sequence Viewer

Length: 720 bp
ATGGAGCTACAAACCCAGCAACAAATCGAGGATGGAGGAAACAACCAAGCAGCCGGGAGTAGTGCCAACACGGATTTCTGGCGAAGCCGTACCAGGTGGATTCCTACTCCAGATCAAATAAGAATCCTCAAGGATCTTTACTACGACAAGGGAGTTAAGACCCCAACTACAGAGCATATTCACGAGATCTGTCTCCAGCTGCAACAGTATGGACAGGTTGAGGGCAAGAACATTTATTTTTGGTTCCAGAATGTCAGGGCTCGAGAGAAGCAGATGAAGAGGTGCAATCAGGCTGCTCAAGTGCCCATGGGAACTAGTTCTCCTGGTACTGGTGGATCCATTGATCTCAATTTTGGGTCCACTGGTTCTACTGGTGCTGGTGGATCCATCGACATCAATTTTGGGCCAGCTGGTGGATCCATTGACATCAATTTTGGGTCCACTAGTTCTACTGGTGCTGGTGGATCCATTGATCTCAATTTTGGTTCCACTGCTTCTACTGGAGGACAATCTTCCATGGAACAACGAGGAGGAGTTCGCCAGGAGTTTGAAACTCTTCCTCTGTTCCCCGTGCACGGCGAGGACGTCTATGGTAACCCGAAGACTACTTCCGAGGAAGGTAGCGCATATGATTACTATTTCGGTGGCTCAGGCGGTTACAACCGTGGATCTCCAGTTTCTCTTGAGCTCAGCCTCAACCCATCCGGAGCTACTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

239

Amino Acids

25.53

Weight (kDa)

4.9

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 30 - 91 4.7e-10 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0032265)

Species Orthologous Gene IDs
rosa_rugosa Rorug01G0194300
rosa_samantha Rh1AG212000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 588
AccB7I CCANNNNNTGG 1 cut(s) 413
AccIII TCCGGA 1 cut(s) 704
AciI CCGC 1 cut(s) 654
AcyI GRCGYC 1 cut(s) 585
AfaI GTAC 2 cut(s) 91, 328
AfiI CCNNNNNNNGG 3 cut(s) 329, 413, 575
AgsI TTSAA 1 cut(s) 551
AhlI ACTAGT 2 cut(s) 314, 443
AjnI CCWGG 3 cut(s) 92, 322, 540
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AloI GAACNNNNNNTCC 2 cut(s) 304, 336
AluBI AGCT 5 cut(s) 7, 199, 410, 688, 710
AluI AGCT 5 cut(s) 7, 199, 410, 688, 710
Alw21I GWGCWC 2 cut(s) 576, 690
Alw26I GTCTC 1 cut(s) 197
Alw44I GTGCAC 1 cut(s) 572
Ama87I CYCGRG 1 cut(s) 261
Aor13HI TCCGGA 1 cut(s) 704
AoxI GGCC 1 cut(s) 404
ApaLI GTGCAC 1 cut(s) 572
ApeKI GCWGC 3 cut(s) 50, 199, 293
ArsI GACNNNNNNTTYG 4 cut(s) 383, 415, 416, 448
Asp700I GAANNNNTTC 2 cut(s) 316, 555
AspLEI GCGC 1 cut(s) 626
AspS9I GGNCC 3 cut(s) 357, 404, 438
AsuC2I CCSGG 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 2 cut(s) 357, 438
BaeGI GKGCMC 2 cut(s) 306, 576
BamHI GGATCC 4 cut(s) 335, 383, 416, 464
BanII GRGCYC 2 cut(s) 262, 690
BauI CACGAG 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 608
Bbv12I GWGCWC 2 cut(s) 576, 690
BbvI GCAGC 3 cut(s) 62, 186, 280
BccI CCATC 3 cut(s) 26, 395, 709
BceAI ACGGC 2 cut(s) 72, 592
BciT130I CCWGG 3 cut(s) 94, 324, 542
BcnI CCSGG 1 cut(s) 55
BcoDI GTCTC 1 cut(s) 197
BcuI ACTAGT 2 cut(s) 314, 443
BfaI CTAG 2 cut(s) 315, 444
BfmI CTRYAG 1 cut(s) 168
BglII AGATCT 1 cut(s) 186
BisI GCNGC 3 cut(s) 51, 200, 294
BlpI GCTNAGC 1 cut(s) 689
BlsI GCNGC 3 cut(s) 52, 201, 295
Bme1390I CCNGG 4 cut(s) 55, 94, 324, 542
Bme18I GGWCC 2 cut(s) 357, 438
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 3 cut(s) 357, 404, 438
BmiI GGNNCC 8 cut(s) 245, 337, 358, 385, 418, 439, 466, 487
BmrFI CCNGG 4 cut(s) 55, 94, 324, 542
BpiI GAAGAC 1 cut(s) 608
BpmI CTGGAG 4 cut(s) 93, 179, 522, 657
Bpu10I CCTNAGC 1 cut(s) 649
Bpu1102I GCTNAGC 1 cut(s) 689
BpuEI CTTGAG 3 cut(s) 113, 282, 704
BpuMI CCSGG 1 cut(s) 55
BsaHI GRCGYC 1 cut(s) 585
BsaJI CCNNGG 4 cut(s) 306, 516, 612, 664
BsaWI WCCGGW 1 cut(s) 704
BsaXI ACNNNNNCTCC 2 cut(s) 304, 334
Bsc4I CCNNNNNNNGG 3 cut(s) 329, 413, 575
Bse1I ACTGG 6 cut(s) 334, 367, 376, 457, 505, 674
BseAI TCCGGA 1 cut(s) 704
BseBI CCWGG 3 cut(s) 94, 324, 542
BseDI CCNNGG 4 cut(s) 306, 516, 612, 664
BseGI GGATG 2 cut(s) 37, 701
BseLI CCNNNNNNNGG 3 cut(s) 329, 413, 575
BseMII CTCAG 2 cut(s) 663, 703
BseNI ACTGG 6 cut(s) 334, 367, 376, 457, 505, 674
BseRI GAGGAG 2 cut(s) 543, 546
BseSI GKGCMC 2 cut(s) 306, 576
BseXI GCAGC 3 cut(s) 62, 186, 280
BseYI CCCAGC 1 cut(s) 15
BshFI GGCC 1 cut(s) 406
BsiHKAI GWGCWC 2 cut(s) 576, 690
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 2 cut(s) 54, 705
BslI CCNNNNNNNGG 3 cut(s) 329, 413, 575
BsmAI GTCTC 1 cut(s) 197
BsnI GGCC 1 cut(s) 406
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 4 cut(s) 262, 306, 576, 690
Bsp13I TCCGGA 1 cut(s) 704
Bsp1720I GCTNAGC 1 cut(s) 689
Bsp19I CCATGG 2 cut(s) 306, 516
BspACI CCGC 1 cut(s) 654
BspANI GGCC 1 cut(s) 406
BspCNI CTCAG 2 cut(s) 662, 702
BspEI TCCGGA 1 cut(s) 704
BspLI GGNNCC 8 cut(s) 245, 337, 358, 385, 418, 439, 466, 487
BsrI ACTGG 6 cut(s) 334, 367, 376, 457, 505, 674
BssECI CCNNGG 4 cut(s) 306, 516, 612, 664
BssNI GRCGYC 1 cut(s) 585
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 2 cut(s) 306, 516
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 3 cut(s) 94, 324, 542
Bst4CI ACNGT 2 cut(s) 207, 665
Bst6I CTCTTC 2 cut(s) 272, 561
BstACI GRCGYC 1 cut(s) 585
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 2 cut(s) 649, 689
BstDSI CCRYGG 3 cut(s) 306, 516, 664
BstEII GGTNACC 1 cut(s) 593
BstF5I GGATG 2 cut(s) 37, 701
BstHHI GCGC 1 cut(s) 626
BstMAI GTCTC 1 cut(s) 197
BstNI CCWGG 3 cut(s) 94, 324, 542
BstPI GGTNACC 1 cut(s) 593
BstSCI CCNGG 4 cut(s) 53, 92, 322, 540
BstSFI CTRYAG 1 cut(s) 168
BstSLI GKGCMC 2 cut(s) 306, 576
BstV1I GCAGC 3 cut(s) 62, 186, 280
BstV2I GAAGAC 1 cut(s) 608
BstX2I RGATCY 7 cut(s) 133, 186, 335, 383, 416, 464, 668
BstYI RGATCY 7 cut(s) 133, 186, 335, 383, 416, 464, 668
BsuRI GGCC 1 cut(s) 406
BtgI CCRYGG 3 cut(s) 306, 516, 664
BtsCI GGATG 2 cut(s) 37, 701
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 2 cut(s) 360, 489
Cac8I GCNNGC 1 cut(s) 408
CfoI GCGC 1 cut(s) 626
Cfr13I GGNCC 3 cut(s) 357, 404, 438
CsiI ACCWGGT 1 cut(s) 92
Csp6I GTAC 2 cut(s) 90, 327
CviAII CATG 2 cut(s) 307, 517
CviQI GTAC 2 cut(s) 90, 327
DdeI CTNAG 2 cut(s) 649, 689
Eam1104I CTCTTC 2 cut(s) 272, 561
EarI CTCTTC 2 cut(s) 272, 561
Ecl136II GAGCTC 1 cut(s) 688
Eco130I CCWWGG 2 cut(s) 306, 516
Eco24I GRGCYC 2 cut(s) 262, 690
Eco47I GGWCC 2 cut(s) 357, 438
Eco53kI GAGCTC 1 cut(s) 688
Eco88I CYCGRG 1 cut(s) 261
Eco91I GGTNACC 1 cut(s) 593
EcoICRI GAGCTC 1 cut(s) 688
EcoO65I GGTNACC 1 cut(s) 593
EcoRII CCWGG 3 cut(s) 92, 322, 540
EcoT14I CCWWGG 2 cut(s) 306, 516
EcoT38I GRGCYC 2 cut(s) 262, 690
ErhI CCWWGG 2 cut(s) 306, 516
FaeI CATG 2 cut(s) 310, 520
FaiI YATR 7 cut(s) 177, 210, 308, 518, 591, 628, 630
FatI CATG 2 cut(s) 306, 516
FauNDI CATATG 1 cut(s) 628
Fnu4HI GCNGC 3 cut(s) 51, 200, 294
FokI GGATG 2 cut(s) 44, 688
FriOI GRGCYC 2 cut(s) 262, 690
Fsp4HI GCNGC 3 cut(s) 51, 200, 294
FspBI CTAG 2 cut(s) 315, 444
GlaI GCGC 1 cut(s) 625
GluI GCNGC 3 cut(s) 51, 200, 294
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 4 cut(s) 93, 179, 522, 657
HaeIII GGCC 1 cut(s) 406
HapII CCGG 2 cut(s) 54, 705
HhaI GCGC 1 cut(s) 626
Hin1I GRCGYC 1 cut(s) 585
Hin1II CATG 2 cut(s) 310, 520
Hin6I GCGC 1 cut(s) 624
HinP1I GCGC 1 cut(s) 624
HinfI GANTC 2 cut(s) 100, 123
HpaII CCGG 2 cut(s) 54, 705
Hpy166II GTNNAC 3 cut(s) 360, 441, 574
Hpy188I TCNGA 1 cut(s) 613
Hpy188III TCNNGA 6 cut(s) 110, 182, 247, 263, 683, 705
Hpy8I GTNNAC 3 cut(s) 360, 441, 574
HpyAV CCTTC 1 cut(s) 611
HpyCH4III ACNGT 2 cut(s) 207, 665
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 3 cut(s) 202, 285, 574
HpyF3I CTNAG 2 cut(s) 649, 689
HpySE526I ACGT 1 cut(s) 585
Hsp92I GRCGYC 1 cut(s) 585
Hsp92II CATG 2 cut(s) 310, 520
HspAI GCGC 1 cut(s) 624
Kpn2I TCCGGA 1 cut(s) 704
LmnI GCTCC 2 cut(s) 4, 707
Lsp1109I GCAGC 3 cut(s) 62, 186, 280
MabI ACCWGGT 1 cut(s) 92
MaeI CTAG 2 cut(s) 315, 444
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 2 cut(s) 593, 656
MboII GAAGA 4 cut(s) 289, 504, 548, 613
MflI RGATCY 7 cut(s) 133, 186, 335, 383, 416, 464, 668
MhlI GDGCHC 4 cut(s) 262, 306, 576, 690
MluCI AATT 4 cut(s) 349, 397, 430, 478
MroI TCCGGA 1 cut(s) 704
MroXI GAANNNNTTC 2 cut(s) 316, 555
MseI TTAA 1 cut(s) 156
MspA1I CMGCKG 2 cut(s) 199, 410
MspI CCGG 2 cut(s) 54, 705
MspR9I CCNGG 4 cut(s) 55, 94, 324, 542
MvaI CCWGG 3 cut(s) 94, 324, 542
NciI CCSGG 1 cut(s) 55
NcoI CCATGG 2 cut(s) 306, 516
NdeI CATATG 1 cut(s) 628
NlaIII CATG 2 cut(s) 310, 520
NlaIV GGNNCC 8 cut(s) 245, 337, 358, 385, 418, 439, 466, 487
PaeR7I CTCGAG 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 582
PdmI GAANNNNTTC 2 cut(s) 316, 555
PfeI GAWTC 2 cut(s) 100, 123
PflMI CCANNNNNTGG 1 cut(s) 413
PkrI GCNGC 3 cut(s) 52, 201, 295
Psp124BI GAGCTC 1 cut(s) 690
Psp6I CCWGG 3 cut(s) 92, 322, 540
PspEI GGTNACC 1 cut(s) 593
PspFI CCCAGC 1 cut(s) 15
PspGI CCWGG 3 cut(s) 92, 322, 540
PspN4I GGNNCC 8 cut(s) 245, 337, 358, 385, 418, 439, 466, 487
PspPI GGNCC 3 cut(s) 357, 404, 438
PsuI RGATCY 7 cut(s) 133, 186, 335, 383, 416, 464, 668
PvuII CAGCTG 2 cut(s) 199, 410
RsaI GTAC 2 cut(s) 91, 328
RsaNI GTAC 2 cut(s) 90, 327
SacI GAGCTC 1 cut(s) 690
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 3 cut(s) 51, 200, 294
Sau96I GGNCC 3 cut(s) 357, 404, 438
ScrFI CCNGG 4 cut(s) 55, 94, 324, 542
SduI GDGCHC 4 cut(s) 262, 306, 576, 690
SexAI ACCWGGT 1 cut(s) 92
SfcI CTRYAG 1 cut(s) 168
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 2 cut(s) 357, 438
SlaI CTCGAG 1 cut(s) 261
SmlI CTYRAG 4 cut(s) 128, 261, 297, 683
SmoI CTYRAG 4 cut(s) 128, 261, 297, 683
SpeI ACTAGT 2 cut(s) 314, 443
Sse9I AATT 4 cut(s) 349, 397, 430, 478
SsiI CCGC 1 cut(s) 654
SspMI CTAG 2 cut(s) 315, 444
SstI GAGCTC 1 cut(s) 690
StyD4I CCNGG 4 cut(s) 53, 92, 322, 540
StyI CCWWGG 2 cut(s) 306, 516
TaaI ACNGT 2 cut(s) 207, 665
TaiI ACGT 1 cut(s) 588
TaqI TCGA 3 cut(s) 27, 262, 390
TasI AATT 4 cut(s) 349, 397, 430, 478
TfiI GAWTC 2 cut(s) 100, 123
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 2 cut(s) 367, 496
TseI GCWGC 3 cut(s) 50, 199, 293
TspDTI ATGAA 1 cut(s) 290
TspGWI ACGGA 1 cut(s) 86
TspRI CASTG 2 cut(s) 367, 496
Van91I CCANNNNNTGG 1 cut(s) 413
VneI GTGCAC 1 cut(s) 572
VpaK11BI GGWCC 2 cut(s) 357, 438
XhoI CTCGAG 1 cut(s) 261
XmnI GAANNNNTTC 2 cut(s) 316, 555
XspI CTAG 2 cut(s) 315, 444
ZraI GACGTC 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.