Rorug05G0439100

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008957
Physical Location & Seq
Forward (+)
61046603 .. 61050659
4057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0439100.1

Sequence Viewer

Length: 981 bp
ATGGAAGTAGCAACCCAAAACTTCAGTCCTGACTGCTTTATAGGTGAAGGAGGATTCGGCAAAGTTTACAAGGGCCACTTAGAGAAAACTAACCAGGTTGTTGCTATCAAGCAGCTTGATCGCAATGGTTGCCAAGGGATCAGGGAATTTGTTGTTGAAGTATTGACGCTAGGTTTGGCAGATCATCACAATCTTGTCAAATTGATTGGCTTTTGTGCTGAGGGGGATCAAAGGCTATTGGTTTATGAATATATGCCATTTGGATCTTTGGAAAACCATTTGCATGATCTTCAAGCTGGCTGGAATGTTCTGGATTGGAATACAAGAATGAAGATCGCAGCTGGTGCAGCAAGAGGTTTAGAGTATCTGCATGAGAAAATGCAGCCTGCTATCATATATCGCGACCTGAAATGCTCAAACATTTTGCTCGGTGAGGATTATCATCCAAAGCTATCTGATTTTGGCTTGGCCAAAGTAGGTCCAAGTGGAGATAAGACTCATGTTTCAACTAGGGTTATGGGAACATATGGGTACTGTGCACCAGATTATGCAATGACAGGTCAGCTGACTTTTAAATCAGATGTTTATAGCTTCGGGGTGGTTCTTTTGGAGCTTATTACAGGCCGAAAAGCTATTGATGGCACAAGACCTCTCAGGGAGCAAAACCTCGTAGCATGGGCAAGACCCTTGTTCAGAGACCGGAAGAAATTCTCACAGATGGTGGATCCGTTGCTCCAGGGTCAGTATCCTGTCAGGGGATTATACCAAGCTCTTGCTATTGCTGCGATGTGCGTTCAGGAGCAGCCTAATATGCGGCCTGTCATAACCGATATAGTGACAGCACTAAATTACCTTGCTTCCCAGAAATATGACCCCCAAGTCCATCCAGTACAAAGCTCCAGAAGGAGTCCATCTTCTCCTAAAGTGAGAAAAGATGATAATATGAGTAGGAATGCGGGTAATGGGCCAGAGAGAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

326

Amino Acids

36.49

Weight (kDa)

8.42

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 13 - 279 2.4e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 14 - 281 1.7e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008957)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26520 AT4G26520 AT4G26520 AT4G26520 AT4G26530 AT4G26530 AT4G26530
fragaria_vesca FvH4_3g42150
malus_domestica MD03G1037700.v1.1 MD11G1038900.v1.1
prunus_persica Prupe.6G029500_v2.0.a1
pyrus_communis pycom11g03290
rosa_chinensis RchiOBHm_Chr5g0075371
rosa_laevigata RLG00000036558
rosa_multiflora Rmu_sc0000770.1_g000022
rosa_roxburghii Rroxscaffold_1G00006120
rosa_rugosa Rorug05G0439100
rosa_samantha Rh5AG495800 Rh5BG517400 Rh5CG541000 Rh5DG531900
rosa_wichuraiana Rw5G046010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 878
AccII CGCG 1 cut(s) 402
AciI CCGC 2 cut(s) 814, 956
AclWI GGATC 5 cut(s) 146, 234, 271, 719, 732
AcoI YGGCCR 1 cut(s) 468
AcsI RAATTY 2 cut(s) 146, 707
AcuI CTGAAG 1 cut(s) 7
AfaI GTAC 2 cut(s) 533, 891
AfiI CCNNNNNNNGG 1 cut(s) 755
AgsI TTSAA 3 cut(s) 158, 293, 507
AjnI CCWGG 2 cut(s) 93, 735
Alw21I GWGCWC 1 cut(s) 541
Alw26I GTCTC 2 cut(s) 690, 969
Alw44I GTGCAC 1 cut(s) 537
AlwI GGATC 5 cut(s) 146, 234, 271, 719, 732
AoxI GGCC 5 cut(s) 73, 468, 622, 815, 965
ApaLI GTGCAC 1 cut(s) 537
ApeKI GCWGC 6 cut(s) 112, 338, 347, 382, 782, 802
ApoI RAATTY 2 cut(s) 146, 707
ArsI GACNNNNNNTTYG 4 cut(s) 10, 42, 157, 189
Asp700I GAANNNNTTC 1 cut(s) 707
AspS9I GGNCC 3 cut(s) 73, 479, 965
AsuHPI GGTGA 2 cut(s) 56, 443
AvaII GGWCC 1 cut(s) 479
BaeGI GKGCMC 1 cut(s) 541
BalI TGGCCA 1 cut(s) 470
BamHI GGATCC 1 cut(s) 724
Bbv12I GWGCWC 1 cut(s) 541
BbvCI CCTCAGC 1 cut(s) 219
BbvI GCAGC 6 cut(s) 124, 350, 359, 394, 769, 814
BccI CCATC 4 cut(s) 632, 712, 891, 919
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 2 cut(s) 95, 737
BciVI GTATCC 1 cut(s) 756
BcoDI GTCTC 2 cut(s) 690, 969
BfaI CTAG 2 cut(s) 170, 510
BfuI GTATCC 1 cut(s) 756
BisI GCNGC 7 cut(s) 113, 339, 348, 383, 783, 803, 815
BlsI GCNGC 7 cut(s) 114, 340, 349, 384, 784, 804, 816
Bme1390I CCNGG 2 cut(s) 95, 737
Bme18I GGWCC 1 cut(s) 479
BmgT120I GGNCC 3 cut(s) 73, 479, 965
BmiI GGNNCC 1 cut(s) 726
BmrFI CCNGG 2 cut(s) 95, 737
BpmI CTGGAG 2 cut(s) 719, 883
Bpu10I CCTNAGC 1 cut(s) 219
BsaBI GATNNNNATC 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 690
BsaJI CCNNGG 2 cut(s) 133, 736
BsaWI WCCGGW 1 cut(s) 699
Bsc4I CCNNNNNNNGG 1 cut(s) 755
Bse1I ACTGG 1 cut(s) 887
Bse3DI GCAATG 2 cut(s) 130, 558
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 2 cut(s) 95, 737
BseDI CCNNGG 2 cut(s) 133, 736
BseGI GGATG 2 cut(s) 442, 883
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 1 cut(s) 755
BseMI GCAATG 2 cut(s) 130, 558
BseMII CTCAG 2 cut(s) 210, 667
BseNI ACTGG 1 cut(s) 887
BseSI GKGCMC 1 cut(s) 541
BseXI GCAGC 6 cut(s) 124, 350, 359, 394, 769, 814
BsgI GTGCAG 1 cut(s) 366
Bsh1236I CGCG 1 cut(s) 402
BshFI GGCC 5 cut(s) 75, 470, 624, 817, 967
BsiHKAI GWGCWC 1 cut(s) 541
BsiSI CCGG 1 cut(s) 700
BslI CCNNNNNNNGG 1 cut(s) 755
BsmAI GTCTC 2 cut(s) 690, 969
BsmI GAATGC 1 cut(s) 958
BsnI GGCC 5 cut(s) 75, 470, 624, 817, 967
Bso31I GGTCTC 1 cut(s) 690
Bsp1286I GDGCHC 1 cut(s) 541
Bsp143I GATC 8 cut(s) 118, 138, 181, 226, 263, 286, 333, 724
Bsp68I TCGCGA 1 cut(s) 402
BspACI CCGC 2 cut(s) 814, 956
BspANI GGCC 5 cut(s) 75, 470, 624, 817, 967
BspCNI CTCAG 2 cut(s) 211, 666
BspFNI CGCG 1 cut(s) 402
BspLI GGNNCC 1 cut(s) 726
BspPI GGATC 5 cut(s) 146, 234, 271, 719, 732
BspTNI GGTCTC 1 cut(s) 690
BsrDI GCAATG 2 cut(s) 130, 558
BsrI ACTGG 1 cut(s) 887
BssECI CCNNGG 2 cut(s) 133, 736
BssMI GATC 8 cut(s) 118, 138, 181, 226, 263, 286, 333, 724
BssT1I CCWWGG 1 cut(s) 133
Bst2UI CCWGG 2 cut(s) 95, 737
Bst4CI ACNGT 1 cut(s) 536
BstAPI GCANNNNNTGC 2 cut(s) 129, 344
BstC8I GCNNGC 2 cut(s) 298, 387
BstDEI CTNAG 3 cut(s) 79, 219, 653
BstF5I GGATG 2 cut(s) 442, 883
BstFNI CGCG 1 cut(s) 402
BstKTI GATC 8 cut(s) 121, 141, 184, 229, 266, 289, 336, 727
BstMAI GTCTC 2 cut(s) 690, 969
BstMBI GATC 8 cut(s) 118, 138, 181, 226, 263, 286, 333, 724
BstMWI GCNNNNNNNGC 5 cut(s) 129, 344, 347, 782, 811
BstNI CCWGG 2 cut(s) 95, 737
BstSCI CCNGG 2 cut(s) 93, 735
BstSLI GKGCMC 1 cut(s) 541
BstUI CGCG 1 cut(s) 402
BstV1I GCAGC 6 cut(s) 124, 350, 359, 394, 769, 814
BstX2I RGATCY 2 cut(s) 263, 724
BstYI RGATCY 2 cut(s) 263, 724
BsuI GTATCC 1 cut(s) 756
BsuRI GGCC 5 cut(s) 75, 470, 624, 817, 967
BtgZI GCGATG 1 cut(s) 800
BtsCI GGATG 2 cut(s) 442, 883
BtuMI TCGCGA 1 cut(s) 402
Cac8I GCNNGC 2 cut(s) 298, 387
Cfr13I GGNCC 3 cut(s) 73, 479, 965
CseI GACGC 1 cut(s) 175
CsiI ACCWGGT 1 cut(s) 93
Csp6I GTAC 2 cut(s) 532, 890
CviAII CATG 4 cut(s) 284, 371, 500, 675
CviQI GTAC 2 cut(s) 532, 890
DdeI CTNAG 3 cut(s) 79, 219, 653
DpnI GATC 8 cut(s) 120, 140, 183, 228, 265, 288, 335, 726
DpnII GATC 8 cut(s) 118, 138, 181, 226, 263, 286, 333, 724
DraI TTTAAA 1 cut(s) 574
DrdI GACNNNNNNGTC 1 cut(s) 878
DseDI GACNNNNNNGTC 1 cut(s) 878
EaeI YGGCCR 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 133
Eco31I GGTCTC 1 cut(s) 690
Eco47I GGWCC 1 cut(s) 479
Eco57I CTGAAG 1 cut(s) 7
EcoRII CCWGG 2 cut(s) 93, 735
EcoT14I CCWWGG 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 133
FaeI CATG 4 cut(s) 287, 374, 503, 678
FalI AAGNNNNNCTT 2 cut(s) 62, 94
FatI CATG 4 cut(s) 283, 370, 499, 674
FauI CCCGC 1 cut(s) 949
FauNDI CATATG 1 cut(s) 526
Fnu4HI GCNGC 7 cut(s) 113, 339, 348, 383, 783, 803, 815
FokI GGATG 2 cut(s) 429, 870
Fsp4HI GCNGC 7 cut(s) 113, 339, 348, 383, 783, 803, 815
FspBI CTAG 2 cut(s) 170, 510
GluI GCNGC 7 cut(s) 113, 339, 348, 383, 783, 803, 815
GsuI CTGGAG 2 cut(s) 719, 883
HaeIII GGCC 5 cut(s) 75, 470, 624, 817, 967
HapII CCGG 1 cut(s) 700
HgaI GACGC 1 cut(s) 175
Hin1II CATG 4 cut(s) 287, 374, 503, 678
HinfI GANTC 3 cut(s) 54, 496, 907
HpaII CCGG 1 cut(s) 700
HphI GGTGA 2 cut(s) 56, 443
Hpy166II GTNNAC 2 cut(s) 67, 539
Hpy188I TCNGA 3 cut(s) 457, 580, 695
Hpy188III TCNNGA 5 cut(s) 29, 311, 401, 797, 900
Hpy8I GTNNAC 2 cut(s) 67, 539
HpyAV CCTTC 2 cut(s) 41, 897
HpyCH4III ACNGT 1 cut(s) 536
HpyCH4V TGCA 6 cut(s) 283, 347, 370, 382, 539, 551
HpyF10VI GCNNNNNNNGC 5 cut(s) 129, 344, 347, 782, 811
HpyF3I CTNAG 3 cut(s) 79, 219, 653
Hsp92II CATG 4 cut(s) 287, 374, 503, 678
Kzo9I GATC 8 cut(s) 118, 138, 181, 226, 263, 286, 333, 724
LmnI GCTCC 5 cut(s) 610, 658, 738, 799, 902
Lsp1109I GCAGC 6 cut(s) 124, 350, 359, 394, 769, 814
MabI ACCWGGT 1 cut(s) 93
MaeI CTAG 2 cut(s) 170, 510
MaeIII GTNAC 1 cut(s) 835
MalI GATC 8 cut(s) 120, 140, 183, 228, 265, 288, 335, 726
MboI GATC 8 cut(s) 118, 138, 181, 226, 263, 286, 333, 724
MboII GAAGA 4 cut(s) 281, 343, 715, 906
MflI RGATCY 2 cut(s) 263, 724
MhlI GDGCHC 1 cut(s) 541
MlsI TGGCCA 1 cut(s) 470
MluCI AATT 4 cut(s) 146, 200, 707, 847
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 2 cut(s) 490, 916
MnlI CCTC 6 cut(s) 44, 214, 347, 427, 660, 677
Mox20I TGGCCA 1 cut(s) 470
MroXI GAANNNNTTC 1 cut(s) 707
MscI TGGCCA 1 cut(s) 470
MseI TTAA 1 cut(s) 573
MslI CAYNNNNRTG 1 cut(s) 282
Msp20I TGGCCA 1 cut(s) 470
MspA1I CMGCKG 2 cut(s) 341, 565
MspI CCGG 1 cut(s) 700
MspR9I CCNGG 2 cut(s) 95, 737
Mva1269I GAATGC 1 cut(s) 958
MvaI CCWGG 2 cut(s) 95, 737
MvnI CGCG 1 cut(s) 402
MwoI GCNNNNNNNGC 5 cut(s) 129, 344, 347, 782, 811
NdeI CATATG 1 cut(s) 526
NdeII GATC 8 cut(s) 118, 138, 181, 226, 263, 286, 333, 724
NlaIII CATG 4 cut(s) 287, 374, 503, 678
NlaIV GGNNCC 1 cut(s) 726
NmuCI GTSAC 1 cut(s) 835
NruI TCGCGA 1 cut(s) 402
PctI GAATGC 1 cut(s) 958
PdmI GAANNNNTTC 1 cut(s) 707
PfeI GAWTC 1 cut(s) 54
PkrI GCNGC 7 cut(s) 114, 340, 349, 384, 784, 804, 816
PleI GAGTC 2 cut(s) 490, 915
PpsI GAGTC 2 cut(s) 490, 915
Psp6I CCWGG 2 cut(s) 93, 735
PspGI CCWGG 2 cut(s) 93, 735
PspN4I GGNNCC 1 cut(s) 726
PspPI GGNCC 3 cut(s) 73, 479, 965
PsuI RGATCY 2 cut(s) 263, 724
PvuII CAGCTG 2 cut(s) 341, 565
RruI TCGCGA 1 cut(s) 402
RsaI GTAC 2 cut(s) 533, 891
RsaNI GTAC 2 cut(s) 532, 890
RseI CAYNNNNRTG 1 cut(s) 282
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 7 cut(s) 113, 339, 348, 383, 783, 803, 815
Sau3AI GATC 8 cut(s) 118, 138, 181, 226, 263, 286, 333, 724
Sau96I GGNCC 3 cut(s) 73, 479, 965
SchI GAGTC 2 cut(s) 490, 916
ScrFI CCNGG 2 cut(s) 95, 737
SduI GDGCHC 1 cut(s) 541
SexAI ACCWGGT 1 cut(s) 93
SinI GGWCC 1 cut(s) 479
SmiMI CAYNNNNRTG 1 cut(s) 282
Sse9I AATT 4 cut(s) 146, 200, 707, 847
SsiI CCGC 2 cut(s) 814, 956
SspMI CTAG 2 cut(s) 170, 510
StyD4I CCNGG 2 cut(s) 93, 735
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 1 cut(s) 536
TasI AATT 4 cut(s) 146, 200, 707, 847
TatI WGTACW 1 cut(s) 889
TauI GCSGC 1 cut(s) 817
TfiI GAWTC 1 cut(s) 54
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TseFI GTSAC 1 cut(s) 835
TseI GCWGC 6 cut(s) 112, 338, 347, 382, 782, 802
Tsp45I GTSAC 1 cut(s) 835
TspDTI ATGAA 2 cut(s) 261, 344
TspGWI ACGGA 1 cut(s) 717
VneI GTGCAC 1 cut(s) 537
VpaK11BI GGWCC 1 cut(s) 479
XapI RAATTY 2 cut(s) 146, 707
XmnI GAANNNNTTC 1 cut(s) 707
XspI CTAG 2 cut(s) 170, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.