Rorug05G0574700

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0023537
Physical Location & Seq
Reverse (-)
76991163 .. 76992323
1161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0574700.1

Sequence Viewer

Length: 1161 bp
ATGGAGGTTGAGTTGATAGCCTGTATCCCTCTTAGCTTACGTGATTCAGAGGACAGATTGATTTGGCACTATGACCAAAAGGGGTGCTATACTGTTCGAAATGGGTATCATGTAGCTAGAGTCGCGGCTAATCGTGTTTCTAGGGCTTCTAGCTCTTCTGGTTCAGTGGGTGTTCAGGGAAAGTACTGGCGGTGTATTTGGAATGCACGTATTCCACCTAAGGTTCGTTTGTTCCTATGGAGACTAATGAGAGGTATCTTACCTCACAAAGTTTCTCTTGCTAGTAGGTTTACTCTTAAGGATATGCGTTGTGCTTTTTGTCATTGTGCTGTTGAGGAGGGTATTCATTTATTTAGAGATTGTGACGTGATTGGGTGGCTATGGATGGAGAGCACTTTGGGTTTAAAAGTGAAGAATGTGCCTGGACGTAGAGTGGAGGAGTGGGTTCTTAATGTGATTGATATTTTGCATGGTGGTCAACGTGATTTGTTCTTCATGGTCTTATGGGTCATATGGACTGAACGCCACAATGCAATTTGGAATGGCACGAGCTTTAATGCTTGTAATGCGGCAAAATGGACTGTGACTCTCCTGGATGATTATCAGAAACAACATCCTGCCGGTTTTTCTAAACACGCTAGACCTAAAGCTAAATGGCAACTTCCCCCTACTGGGCGTCTGAAGATTAATGTTGATGGAAGCTATAGACCAGAGTGTGGTGATGGTGGCATTGGGGTGGTCGTTCGAGATGAGGCTGGTAATTGCATTGCAGCAATGGCACGTTATTTCCCTCATGTTCTTTCTGCTATTCACATGGAAGCGGAGGCTTGTAGGGTTGGAATTCTTTTGGCTATTCACCAATATTGGAATGCCTTTGACCTTGAAAGTGATTGCTCACTTGTGGTGGCTGCATTGCAACTGAATGTGGAAGATCGATCTGAGGTAGGGCGCATTATCGATGACTGTAAGTCATATTTGACATCTTTACACTCTATTCAGATTAGACATATCTTCCGTGAAGCAAATAGTGTAGCAAATAGATTAGCTCACCTTGCTAGTTTGAACTATCTAGATGATATTTGGTTAGAGTCTCCTGTGATTATCCGGGATGTACTCTATGAGGATTCTTGTACTACTGCTAGAGTCTATGCATATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

386

Amino Acids

44.04

Weight (kDa)

8.34

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 31 - 128 1.9e-13 zinc-binding in reverse transcriptase
RVT_3 PF13456 230 - 351 1e-21 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023537)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001228.1_g000012
rosa_rugosa Rorug05G0574600 Rorug05G0574700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 716
AccII CGCG 1 cut(s) 125
AciI CCGC 4 cut(s) 125, 190, 569, 821
AcsI RAATTY 1 cut(s) 840
AcuI CTGAAG 1 cut(s) 701
AcyI GRCGYC 1 cut(s) 676
AfaI GTAC 3 cut(s) 185, 1113, 1132
AfiI CCNNNNNNNGG 3 cut(s) 671, 672, 716
AflII CTTAAG 1 cut(s) 296
AgsI TTSAA 2 cut(s) 884, 1063
AhdI GACNNNNNGTC 1 cut(s) 967
AjiI CACGTC 1 cut(s) 367
AjnI CCWGG 2 cut(s) 421, 591
AluBI AGCT 7 cut(s) 36, 116, 153, 552, 650, 702, 1046
AluI AGCT 7 cut(s) 36, 116, 153, 552, 650, 702, 1046
Alw21I GWGCWC 1 cut(s) 395
Alw26I GTCTC 2 cut(s) 235, 1095
ApeKI GCWGC 2 cut(s) 770, 908
ApoI RAATTY 1 cut(s) 840
AseI ATTAAT 1 cut(s) 687
AspLEI GCGC 1 cut(s) 951
AsuC2I CCSGG 1 cut(s) 1106
AsuHPI GGTGA 3 cut(s) 731, 848, 1040
AsuII TTCGAA 1 cut(s) 97
AxyI CCTNAGG 1 cut(s) 219
BauI CACGAG 1 cut(s) 547
Bbv12I GWGCWC 1 cut(s) 395
BbvI GCAGC 2 cut(s) 782, 895
BccI CCATC 3 cut(s) 379, 689, 716
BciT130I CCWGG 2 cut(s) 423, 593
BciVI GTATCC 1 cut(s) 35
BcnI CCSGG 1 cut(s) 1106
BcoDI GTCTC 2 cut(s) 235, 1095
BfaI CTAG 8 cut(s) 117, 141, 150, 282, 639, 1056, 1070, 1140
BfmI CTRYAG 1 cut(s) 703
BfrI CTTAAG 1 cut(s) 296
BfuI GTATCC 1 cut(s) 35
BisI GCNGC 4 cut(s) 126, 570, 771, 909
BlsI GCNGC 4 cut(s) 127, 571, 772, 910
BmcAI AGTACT 1 cut(s) 185
Bme1390I CCNGG 3 cut(s) 423, 593, 1106
BmeRI GACNNNNNGTC 1 cut(s) 967
BmgBI CACGTC 1 cut(s) 367
BmrFI CCNGG 3 cut(s) 423, 593, 1106
BmrI ACTGGG 1 cut(s) 681
BmuI ACTGGG 1 cut(s) 681
Bpu14I TTCGAA 1 cut(s) 97
BpuMI CCSGG 1 cut(s) 1106
Bsa29I ATCGAT 2 cut(s) 934, 957
BsaAI YACGTR 2 cut(s) 41, 209
BsaBI GATNNNNATC 1 cut(s) 600
BsaHI GRCGYC 1 cut(s) 676
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 671, 672, 716
Bse118I RCCGGY 1 cut(s) 620
Bse1I ACTGG 2 cut(s) 191, 676
Bse21I CCTNAGG 1 cut(s) 219
Bse3DI GCAATG 3 cut(s) 765, 780, 911
Bse8I GATNNNNATC 1 cut(s) 600
BseBI CCWGG 2 cut(s) 423, 593
BseCI ATCGAT 2 cut(s) 934, 957
BseGI GGATG 4 cut(s) 390, 601, 613, 1114
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 3 cut(s) 671, 672, 716
BseMI GCAATG 3 cut(s) 765, 780, 911
BseMII CTCAG 1 cut(s) 930
BseNI ACTGG 2 cut(s) 191, 676
BseRI GAGGAG 2 cut(s) 350, 452
BseXI GCAGC 2 cut(s) 782, 895
Bsh1236I CGCG 1 cut(s) 125
BshVI ATCGAT 2 cut(s) 934, 957
BsiHKAI GWGCWC 1 cut(s) 395
BsiSI CCGG 2 cut(s) 621, 1105
BslI CCNNNNNNNGG 3 cut(s) 671, 672, 716
BsmAI GTCTC 2 cut(s) 235, 1095
BsmI GAATGC 2 cut(s) 208, 874
Bsp119I TTCGAA 1 cut(s) 97
Bsp1286I GDGCHC 1 cut(s) 395
Bsp143I GATC 2 cut(s) 931, 935
BspACI CCGC 4 cut(s) 125, 190, 569, 821
BspCNI CTCAG 1 cut(s) 931
BspDI ATCGAT 2 cut(s) 934, 957
BspFNI CGCG 1 cut(s) 125
BspQI GCTCTTC 1 cut(s) 160
BspT104I TTCGAA 1 cut(s) 97
BspTI CTTAAG 1 cut(s) 296
BsrDI GCAATG 3 cut(s) 765, 780, 911
BsrFI RCCGGY 1 cut(s) 620
BsrI ACTGG 2 cut(s) 191, 676
BssAI RCCGGY 1 cut(s) 620
BssMI GATC 2 cut(s) 931, 935
BssNI GRCGYC 1 cut(s) 676
BssSI CACGAG 1 cut(s) 547
Bst2BI CACGAG 1 cut(s) 547
Bst2UI CCWGG 2 cut(s) 423, 593
Bst4CI ACNGT 3 cut(s) 94, 583, 965
Bst6I CTCTTC 1 cut(s) 160
BstACI GRCGYC 1 cut(s) 676
BstAFI CTTAAG 1 cut(s) 296
BstBAI YACGTR 2 cut(s) 41, 209
BstBI TTCGAA 1 cut(s) 97
BstDEI CTNAG 3 cut(s) 32, 219, 939
BstF5I GGATG 4 cut(s) 390, 601, 613, 1114
BstFNI CGCG 1 cut(s) 125
BstHHI GCGC 1 cut(s) 951
BstKTI GATC 2 cut(s) 934, 938
BstMAI GTCTC 2 cut(s) 235, 1095
BstMBI GATC 2 cut(s) 931, 935
BstMWI GCNNNNNNNGC 4 cut(s) 122, 566, 776, 1052
BstNI CCWGG 2 cut(s) 423, 593
BstSCI CCNGG 3 cut(s) 421, 591, 1104
BstSFI CTRYAG 1 cut(s) 703
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 2 cut(s) 782, 895
Bsu15I ATCGAT 2 cut(s) 934, 957
Bsu36I CCTNAGG 1 cut(s) 219
BsuI GTATCC 1 cut(s) 35
BsuTUI ATCGAT 2 cut(s) 934, 957
BtrI CACGTC 1 cut(s) 367
BtsCI GGATG 4 cut(s) 390, 601, 613, 1114
BtsIMutI CAGTG 1 cut(s) 171
CfoI GCGC 1 cut(s) 951
Cfr10I RCCGGY 1 cut(s) 620
ClaI ATCGAT 2 cut(s) 934, 957
CseI GACGC 1 cut(s) 665
Csp6I GTAC 3 cut(s) 184, 1112, 1131
CviAII CATG 5 cut(s) 110, 470, 496, 794, 814
CviQI GTAC 3 cut(s) 184, 1112, 1131
DdeI CTNAG 3 cut(s) 32, 219, 939
DpnI GATC 2 cut(s) 933, 937
DpnII GATC 2 cut(s) 931, 935
DraI TTTAAA 1 cut(s) 405
DriI GACNNNNNGTC 1 cut(s) 967
Eam1104I CTCTTC 1 cut(s) 160
Eam1105I GACNNNNNGTC 1 cut(s) 967
EarI CTCTTC 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 701
Eco81I CCTNAGG 1 cut(s) 219
EcoRI GAATTC 1 cut(s) 840
EcoRII CCWGG 2 cut(s) 421, 591
EcoT22I ATGCAT 1 cut(s) 1153
FaeI CATG 5 cut(s) 113, 473, 499, 797, 817
FalI AAGNNNNNCTT 2 cut(s) 261, 293
FatI CATG 5 cut(s) 109, 469, 495, 793, 813
FauNDI CATATG 1 cut(s) 512
Fnu4HI GCNGC 4 cut(s) 126, 570, 771, 909
FokI GGATG 4 cut(s) 397, 600, 608, 1121
Fsp4HI GCNGC 4 cut(s) 126, 570, 771, 909
FspBI CTAG 8 cut(s) 117, 141, 150, 282, 639, 1056, 1070, 1140
GlaI GCGC 1 cut(s) 950
GluI GCNGC 4 cut(s) 126, 570, 771, 909
HapII CCGG 2 cut(s) 621, 1105
HgaI GACGC 1 cut(s) 665
HhaI GCGC 1 cut(s) 951
Hin1I GRCGYC 1 cut(s) 676
Hin1II CATG 5 cut(s) 113, 473, 499, 797, 817
Hin6I GCGC 1 cut(s) 949
HinP1I GCGC 1 cut(s) 949
HincII GTYRAC 1 cut(s) 479
HindII GTYRAC 1 cut(s) 479
HinfI GANTC 6 cut(s) 44, 120, 586, 1088, 1124, 1143
HpaII CCGG 2 cut(s) 621, 1105
HphI GGTGA 3 cut(s) 731, 848, 1040
Hpy166II GTNNAC 2 cut(s) 291, 479
Hpy188I TCNGA 5 cut(s) 49, 606, 681, 940, 999
Hpy188III TCNNGA 2 cut(s) 746, 1070
Hpy8I GTNNAC 2 cut(s) 291, 479
HpyCH4III ACNGT 3 cut(s) 94, 583, 965
HpyCH4IV ACGT 6 cut(s) 40, 208, 366, 427, 481, 781
HpyCH4V TGCA 8 cut(s) 206, 469, 533, 765, 770, 911, 916, 1151
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 566, 776, 1052
HpyF3I CTNAG 3 cut(s) 32, 219, 939
HpySE526I ACGT 6 cut(s) 40, 208, 366, 427, 481, 781
Hsp92I GRCGYC 1 cut(s) 676
Hsp92II CATG 5 cut(s) 113, 473, 499, 797, 817
HspAI GCGC 1 cut(s) 949
Kzo9I GATC 2 cut(s) 931, 935
LguI GCTCTTC 1 cut(s) 160
Lsp1109I GCAGC 2 cut(s) 782, 895
MaeI CTAG 8 cut(s) 117, 141, 150, 282, 639, 1056, 1070, 1140
MaeII ACGT 6 cut(s) 40, 208, 366, 427, 481, 781
MaeIII GTNAC 2 cut(s) 362, 583
MalI GATC 2 cut(s) 933, 937
MboI GATC 2 cut(s) 931, 935
MboII GAAGA 6 cut(s) 147, 424, 484, 694, 941, 1003
MhlI GDGCHC 1 cut(s) 395
MluCI AATT 3 cut(s) 534, 760, 840
MlyI GAGTC 4 cut(s) 129, 580, 1097, 1152
MmeI TCCRAC 1 cut(s) 817
Mph1103I ATGCAT 1 cut(s) 1153
MseI TTAA 5 cut(s) 297, 404, 450, 555, 687
MslI CAYNNNNRTG 1 cut(s) 734
MspCI CTTAAG 1 cut(s) 296
MspI CCGG 2 cut(s) 621, 1105
MspR9I CCNGG 3 cut(s) 423, 593, 1106
Mva1269I GAATGC 2 cut(s) 208, 874
MvaI CCWGG 2 cut(s) 423, 593
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 4 cut(s) 122, 566, 776, 1052
NciI CCSGG 1 cut(s) 1106
NdeI CATATG 1 cut(s) 512
NdeII GATC 2 cut(s) 931, 935
NlaIII CATG 5 cut(s) 113, 473, 499, 797, 817
NmuCI GTSAC 2 cut(s) 362, 583
NsiI ATGCAT 1 cut(s) 1153
NspV TTCGAA 1 cut(s) 97
PciSI GCTCTTC 1 cut(s) 160
PctI GAATGC 2 cut(s) 208, 874
PfeI GAWTC 2 cut(s) 44, 1124
PflMI CCANNNNNTGG 1 cut(s) 716
PfoI TCCNGGA 2 cut(s) 591, 1104
PkrI GCNGC 4 cut(s) 127, 571, 772, 910
PleI GAGTC 4 cut(s) 128, 580, 1096, 1151
PpsI GAGTC 4 cut(s) 128, 580, 1096, 1151
Ppu21I YACGTR 2 cut(s) 41, 209
PshBI ATTAAT 1 cut(s) 687
Psp6I CCWGG 2 cut(s) 421, 591
PspGI CCWGG 2 cut(s) 421, 591
RsaI GTAC 3 cut(s) 185, 1113, 1132
RsaNI GTAC 3 cut(s) 184, 1112, 1131
RseI CAYNNNNRTG 1 cut(s) 734
SapI GCTCTTC 1 cut(s) 160
SaqAI TTAA 5 cut(s) 297, 404, 450, 555, 687
SatI GCNGC 4 cut(s) 126, 570, 771, 909
Sau3AI GATC 2 cut(s) 931, 935
ScaI AGTACT 1 cut(s) 185
SchI GAGTC 4 cut(s) 129, 580, 1097, 1152
ScrFI CCNGG 3 cut(s) 423, 593, 1106
SduI GDGCHC 1 cut(s) 395
SfcI CTRYAG 1 cut(s) 703
SfuI TTCGAA 1 cut(s) 97
SmiMI CAYNNNNRTG 1 cut(s) 734
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 3 cut(s) 534, 760, 840
SsiI CCGC 4 cut(s) 125, 190, 569, 821
SspI AATATT 1 cut(s) 863
SspMI CTAG 8 cut(s) 117, 141, 150, 282, 639, 1056, 1070, 1140
StyD4I CCNGG 3 cut(s) 421, 591, 1104
TaaI ACNGT 3 cut(s) 94, 583, 965
TaiI ACGT 6 cut(s) 43, 211, 369, 430, 484, 784
TaqI TCGA 4 cut(s) 97, 745, 934, 957
TasI AATT 3 cut(s) 534, 760, 840
TatI WGTACW 3 cut(s) 183, 1111, 1130
TauI GCSGC 2 cut(s) 128, 572
TfiI GAWTC 2 cut(s) 44, 1124
Tru1I TTAA 5 cut(s) 297, 404, 450, 555, 687
Tru9I TTAA 5 cut(s) 297, 404, 450, 555, 687
TscAI CASTG 1 cut(s) 171
TseFI GTSAC 2 cut(s) 362, 583
TseI GCWGC 2 cut(s) 770, 908
Tsp45I GTSAC 2 cut(s) 362, 583
TspDTI ATGAA 2 cut(s) 335, 484
TspGWI ACGGA 1 cut(s) 1004
TspRI CASTG 1 cut(s) 171
Van91I CCANNNNNTGG 1 cut(s) 716
Vha464I CTTAAG 1 cut(s) 296
VspI ATTAAT 1 cut(s) 687
XapI RAATTY 1 cut(s) 840
XbaI TCTAGA 1 cut(s) 1069
XspI CTAG 8 cut(s) 117, 141, 150, 282, 639, 1056, 1070, 1140
ZrmI AGTACT 1 cut(s) 185
Zsp2I ATGCAT 1 cut(s) 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.