Rorug06G0081700

tRNA synthetases class II (A)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0030189
Physical Location & Seq
Forward (+)
10883489 .. 10883966
478 bp
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UTR
Exon/CDS
Intron
Rorug06G0081700.1

Sequence Viewer

Length: 387 bp
ATGGCAAACCCTCACGCACCACCGCCTCGACCTCTCTACAAGCAGAACTCCTGGTCGCCGGACATACTCCGTGAGGAGACATGGCAGCGTCAGAAGAGCAATTGGGGTACGCGGAGTCTCAACCGCAGCAAAAGCGTTTCAGACGATGACTTGGAGGAACTAAAAGCCTGCATTGAGCTGGGGTTCGGGTTTGATTCGCCAGAAATCGACCCCAAACTCTGCCGCACCTTACCTGCATTGGAGTCTTACCATGCTTTAAACAAGCAGCGGTTTGGTAAAAGCGTATCAACAGGAGGGGATGATGCGGAGGTGGTGAAGATGAGGTTAAAGCAGTGGGCGCAATTGGTTGCCTGTGCGGTTCGGGAATCATCTTCTTCCGGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA-synt_2c tRNA_SAD

Protein Analysis

128

Amino Acids

14.43

Weight (kDa)

8.49

Isoelectric Point (pI)

68.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 46 - 91 1.4e-21 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0030189)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0008751
rosa_rugosa Rorug06G0081700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 241
AccII CGCG 1 cut(s) 112
AciI CCGC 7 cut(s) 23, 112, 124, 223, 268, 305, 356
AfaI GTAC 1 cut(s) 109
AjnI CCWGG 1 cut(s) 50
AluBI AGCT 1 cut(s) 178
AluI AGCT 1 cut(s) 178
Alw26I GTCTC 2 cut(s) 71, 122
ApeKI GCWGC 3 cut(s) 85, 126, 265
AspLEI GCGC 1 cut(s) 340
AsuC2I CCSGG 1 cut(s) 379
AsuHPI GGTGA 1 cut(s) 325
BbvI GCAGC 3 cut(s) 97, 138, 277
BciT130I CCWGG 1 cut(s) 52
BcnI CCSGG 1 cut(s) 379
BcoDI GTCTC 2 cut(s) 71, 122
BfuAI ACCTGC 1 cut(s) 241
BisI GCNGC 4 cut(s) 86, 127, 223, 266
BlsI GCNGC 4 cut(s) 87, 128, 224, 267
Bme1390I CCNGG 2 cut(s) 52, 379
BmrFI CCNGG 2 cut(s) 52, 379
BmsI GCATC 1 cut(s) 292
BpuMI CCSGG 1 cut(s) 379
BseBI CCWGG 1 cut(s) 52
BseGI GGATG 1 cut(s) 304
BseRI GAGGAG 1 cut(s) 89
BseXI GCAGC 3 cut(s) 97, 138, 277
BseYI CCCAGC 1 cut(s) 178
Bsh1236I CGCG 1 cut(s) 112
BsiSI CCGG 2 cut(s) 59, 378
BsmAI GTCTC 2 cut(s) 71, 122
BspACI CCGC 7 cut(s) 23, 112, 124, 223, 268, 305, 356
BspFNI CGCG 1 cut(s) 112
BspMI ACCTGC 1 cut(s) 241
BspQI GCTCTTC 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 52
Bst6I CTCTTC 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 169
BstF5I GGATG 1 cut(s) 304
BstFNI CGCG 1 cut(s) 112
BstHHI GCGC 1 cut(s) 340
BstMAI GTCTC 2 cut(s) 71, 122
BstMWI GCNNNNNNNGC 2 cut(s) 132, 337
BstNI CCWGG 1 cut(s) 52
BstSCI CCNGG 2 cut(s) 50, 377
BstUI CGCG 1 cut(s) 112
BstV1I GCAGC 3 cut(s) 97, 138, 277
BtsCI GGATG 1 cut(s) 304
BtsI GCAGTG 1 cut(s) 338
BtsIMutI CAGTG 1 cut(s) 338
BveI ACCTGC 1 cut(s) 241
Cac8I GCNNGC 1 cut(s) 169
CfoI GCGC 1 cut(s) 340
CseI GACGC 1 cut(s) 77
Csp6I GTAC 1 cut(s) 108
CviAII CATG 2 cut(s) 81, 251
CviJI RGCY 2 cut(s) 167, 178
CviKI_1 RGCY 2 cut(s) 167, 178
CviQI GTAC 1 cut(s) 108
DraI TTTAAA 1 cut(s) 258
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 50
FaeI CATG 2 cut(s) 84, 254
FaiI YATR 3 cut(s) 65, 82, 252
FatI CATG 2 cut(s) 80, 250
Fnu4HI GCNGC 4 cut(s) 86, 127, 223, 266
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 4 cut(s) 86, 127, 223, 266
GlaI GCGC 1 cut(s) 339
GluI GCNGC 4 cut(s) 86, 127, 223, 266
GsaI CCCAGC 1 cut(s) 182
HapII CCGG 2 cut(s) 59, 378
HgaI GACGC 1 cut(s) 77
HhaI GCGC 1 cut(s) 340
Hin1II CATG 2 cut(s) 84, 254
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HinfI GANTC 4 cut(s) 115, 194, 242, 365
HpaII CCGG 2 cut(s) 59, 378
HphI GGTGA 1 cut(s) 325
Hpy188I TCNGA 2 cut(s) 93, 142
Hpy188III TCNNGA 1 cut(s) 362
HpyCH4V TGCA 2 cut(s) 171, 236
HpyF10VI GCNNNNNNNGC 2 cut(s) 132, 337
Hsp92II CATG 2 cut(s) 84, 254
HspAI GCGC 1 cut(s) 338
LguI GCTCTTC 1 cut(s) 89
LpnPI CCDG 9 cut(s) 37, 64, 72, 164, 181, 213, 246, 276, 364
Lsp1109I GCAGC 3 cut(s) 97, 138, 277
LweI GCATC 1 cut(s) 292
MboII GAAGA 4 cut(s) 106, 328, 363, 366
MfeI CAATTG 2 cut(s) 100, 341
MluCI AATT 2 cut(s) 100, 341
MlyI GAGTC 2 cut(s) 124, 251
MnlI CCTC 8 cut(s) 21, 36, 42, 67, 148, 287, 301, 315
MseI TTAA 2 cut(s) 257, 326
MspA1I CMGCKG 1 cut(s) 268
MspI CCGG 2 cut(s) 59, 378
MspR9I CCNGG 2 cut(s) 52, 379
MunI CAATTG 2 cut(s) 100, 341
MvaI CCWGG 1 cut(s) 52
MvnI CGCG 1 cut(s) 112
MwoI GCNNNNNNNGC 2 cut(s) 132, 337
NciI CCSGG 1 cut(s) 379
NlaIII CATG 2 cut(s) 84, 254
PciSI GCTCTTC 1 cut(s) 89
PfeI GAWTC 2 cut(s) 194, 365
PfoI TCCNGGA 1 cut(s) 377
PkrI GCNGC 4 cut(s) 87, 128, 224, 267
PleI GAGTC 2 cut(s) 123, 250
PpsI GAGTC 2 cut(s) 123, 250
Psp6I CCWGG 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 178
PspGI CCWGG 1 cut(s) 50
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
SapI GCTCTTC 1 cut(s) 89
SaqAI TTAA 2 cut(s) 257, 326
SatI GCNGC 4 cut(s) 86, 127, 223, 266
SchI GAGTC 2 cut(s) 124, 251
ScrFI CCNGG 2 cut(s) 52, 379
SetI ASST 6 cut(s) 34, 180, 230, 235, 312, 326
SfaNI GCATC 1 cut(s) 292
Sse9I AATT 2 cut(s) 100, 341
SsiI CCGC 7 cut(s) 23, 112, 124, 223, 268, 305, 356
StyD4I CCNGG 2 cut(s) 50, 377
TaqI TCGA 2 cut(s) 28, 207
TasI AATT 2 cut(s) 100, 341
TauI GCSGC 1 cut(s) 225
TfiI GAWTC 2 cut(s) 194, 365
Tru1I TTAA 2 cut(s) 257, 326
Tru9I TTAA 2 cut(s) 257, 326
TscAI CASTG 1 cut(s) 338
TseI GCWGC 3 cut(s) 85, 126, 265
TspGWI ACGGA 1 cut(s) 59
TspRI CASTG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.