Rorug06G0218800

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0020566
Physical Location & Seq
Forward (+)
34933801 .. 34936888
3088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0218800.1

Sequence Viewer

Length: 957 bp
ATGGAATTATTAAGCACAGTATGGAATTCCAGAGCCTCTGCTATCTCATTTGCACCACTCATCACTCCCAATACTTGCACTACCTCTCACTTCCAACACCCTTCTCTCCCACCTCACAAGCTTTCTTTCCCATTTTGCCCGTACCCATTTCGCTTTGTTCCCAGCAAGTCCCTAACACTGCAGGCTCGCAAGCGAAACTCGGAGCCTGAGCAGCAGCCACTTGTCAGCCCAACCATTATTGAAGAGGTTTCAGAGGATGATGAAGACGACGACGACGTTATCTTGGATGACTTTGATGATGAGGCATCAGTGGACGATGATTATTATGGGGATGAGTCTGCGGCGGATGAGGCTGAACTGTATGATGGAGATGGAGGCGGAGGAGGTGGAATTTCTCTTGCGGGGACATGGTGGGATGAAAAAGCATTGGGAATAGCTGAGGAAGTTATTCTGACCTTTGAGGGTGACTTGAAAATATATGCCTTTAAAACATTGCCAAATTTCAACATTCAAGTGCGCATTGAGAAGCTTTCGAATAAATCTGGCTCCCCAAGTATGGAAGACATAGAAGCTTTTTCTAGAACCTATAGAACAAGACTGGACGAAGCAGAGCTTGCTCGATCAGTACCTGAGAACTTATGTCTTGAGGTGTCATCTCCAGGTGTAGAAAGGGTAGTTCGGATTCCACAAGATTTGGATCGGTATAAAGATCGACCTATGTTTGTAAAATATGTAACTGAGACGGATGCAACTGGTTCATTATCAGAAAGTGATGGTGTTTTCAGGCTTGTTTCTTTTGATATGGAAACAAAATGTTGCATCTGGGGTGTAGCAGACGTGAGGATTAATAGAGAAAAAGCAGGAAAAGGGAGACCACTTAACAAAAAGCAGCGGGAGTGGCGATTGAATACACCCTTTGATTCCTTACGTTTAGTCCGGTTGTATTCTGATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

318

Amino Acids

35.84

Weight (kDa)

4.57

Isoelectric Point (pI)

50.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RimP_N PF02576 168 - 223 9e-06 RimP N-terminal domain
DUF7912 PF25498 225 - 315 4.5e-38 Domain of unknown function (DUF7912)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020566)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0291311
rosa_laevigata RLG00000012124
rosa_rugosa Rorug06G0218800
rosa_samantha Rh6AG330700 Rh6DG331000
rosa_wichuraiana Rw6G028720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 518
AciI CCGC 5 cut(s) 341, 344, 378, 401, 892
AclWI GGATC 1 cut(s) 705
AcsI RAATTY 3 cut(s) 25, 390, 499
AfaI GTAC 2 cut(s) 143, 627
AfiI CCNNNNNNNGG 1 cut(s) 556
AgsI TTSAA 5 cut(s) 242, 472, 505, 512, 907
AjiI CACGTC 1 cut(s) 838
AjnI CCWGG 1 cut(s) 658
AluBI AGCT 5 cut(s) 121, 437, 529, 572, 613
AluI AGCT 5 cut(s) 121, 437, 529, 572, 613
Alw26I GTCTC 2 cut(s) 734, 865
AlwI GGATC 1 cut(s) 705
AlwNI CAGNNNCTG 1 cut(s) 629
ApeKI GCWGC 3 cut(s) 211, 214, 889
ApoI RAATTY 3 cut(s) 25, 390, 499
AseI ATTAAT 1 cut(s) 846
Asp700I GAANNNNTTC 1 cut(s) 447
AspLEI GCGC 1 cut(s) 519
AsuHPI GGTGA 1 cut(s) 476
AsuII TTCGAA 1 cut(s) 533
BbsI GAAGAC 2 cut(s) 270, 567
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 3 cut(s) 223, 226, 901
BccI CCATC 3 cut(s) 359, 365, 767
BciT130I CCWGG 1 cut(s) 660
BcoDI GTCTC 2 cut(s) 734, 865
BfaI CTAG 1 cut(s) 579
BfmI CTRYAG 2 cut(s) 179, 586
BisI GCNGC 4 cut(s) 212, 215, 342, 890
BlsI GCNGC 4 cut(s) 213, 216, 343, 891
Bme1390I CCNGG 1 cut(s) 660
BmgBI CACGTC 1 cut(s) 838
BmiI GGNNCC 2 cut(s) 204, 547
BmrFI CCNGG 1 cut(s) 660
BmsI GCATC 3 cut(s) 314, 736, 828
BpiI GAAGAC 2 cut(s) 270, 567
BpmI CTGGAG 1 cut(s) 642
Bpu10I CCTNAGC 2 cut(s) 207, 438
Bpu14I TTCGAA 1 cut(s) 533
BpuEI CTTGAG 1 cut(s) 665
BsaBI GATNNNNATC 1 cut(s) 696
BsaI GGTCTC 1 cut(s) 865
BsaWI WCCGGW 1 cut(s) 936
Bsc4I CCNNNNNNNGG 1 cut(s) 556
Bse1I ACTGG 2 cut(s) 603, 757
Bse3DI GCAATG 1 cut(s) 491
Bse8I GATNNNNATC 1 cut(s) 696
BseBI CCWGG 1 cut(s) 660
BseGI GGATG 6 cut(s) 262, 292, 337, 352, 421, 751
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 1 cut(s) 556
BseMI GCAATG 1 cut(s) 491
BseMII CTCAG 4 cut(s) 198, 429, 621, 729
BseNI ACTGG 2 cut(s) 603, 757
BseRI GAGGAG 1 cut(s) 396
BseXI GCAGC 3 cut(s) 223, 226, 901
BseYI CCCAGC 1 cut(s) 161
BsiSI CCGG 1 cut(s) 937
BslFI GGGAC 2 cut(s) 154, 418
BslI CCNNNNNNNGG 1 cut(s) 556
BsmAI GTCTC 2 cut(s) 734, 865
BsmBI CGTCTC 1 cut(s) 734
BsmFI GGGAC 2 cut(s) 154, 418
Bso31I GGTCTC 1 cut(s) 865
Bsp119I TTCGAA 1 cut(s) 533
Bsp143I GATC 3 cut(s) 620, 697, 709
BspACI CCGC 5 cut(s) 341, 344, 378, 401, 892
BspCNI CTCAG 4 cut(s) 199, 430, 622, 730
BspLI GGNNCC 2 cut(s) 204, 547
BspMAI CTGCAG 1 cut(s) 183
BspPI GGATC 1 cut(s) 705
BspT104I TTCGAA 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 865
BsrDI GCAATG 1 cut(s) 491
BsrI ACTGG 2 cut(s) 603, 757
BssMI GATC 3 cut(s) 620, 697, 709
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 2 cut(s) 19, 360
Bst6I CTCTTC 1 cut(s) 237
BstAPI GCANNNNNTGC 1 cut(s) 614
BstBI TTCGAA 1 cut(s) 533
BstC8I GCNNGC 4 cut(s) 183, 187, 191, 615
BstDEI CTNAG 4 cut(s) 207, 438, 630, 738
BstF5I GGATG 6 cut(s) 262, 292, 337, 352, 421, 751
BstHHI GCGC 1 cut(s) 519
BstKTI GATC 3 cut(s) 623, 700, 712
BstMAI GTCTC 2 cut(s) 734, 865
BstMBI GATC 3 cut(s) 620, 697, 709
BstMWI GCNNNNNNNGC 4 cut(s) 211, 350, 614, 898
BstNI CCWGG 1 cut(s) 660
BstSCI CCNGG 1 cut(s) 658
BstSFI CTRYAG 2 cut(s) 179, 586
BstV1I GCAGC 3 cut(s) 223, 226, 901
BstV2I GAAGAC 2 cut(s) 270, 567
BtrI CACGTC 1 cut(s) 838
BtsCI GGATG 6 cut(s) 262, 292, 337, 352, 421, 751
BtsI GCAGTG 1 cut(s) 176
BtsIMutI CAGTG 2 cut(s) 176, 315
Cac8I GCNNGC 4 cut(s) 183, 187, 191, 615
CaiI CAGNNNCTG 1 cut(s) 629
CfoI GCGC 1 cut(s) 519
Csp6I GTAC 2 cut(s) 142, 626
CspCI CAANNNNNGTGG 2 cut(s) 675, 710
CviAII CATG 1 cut(s) 408
CviQI GTAC 2 cut(s) 142, 626
DdeI CTNAG 4 cut(s) 207, 438, 630, 738
DpnI GATC 3 cut(s) 622, 699, 711
DpnII GATC 3 cut(s) 620, 697, 709
DraI TTTAAA 1 cut(s) 487
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
EciI GGCGGA 2 cut(s) 359, 393
Eco31I GGTCTC 1 cut(s) 865
EcoRI GAATTC 1 cut(s) 25
EcoRII CCWGG 1 cut(s) 658
Esp3I CGTCTC 1 cut(s) 734
FaeI CATG 1 cut(s) 411
FalI AAGNNNNNCTT 2 cut(s) 597, 629
FaqI GGGAC 2 cut(s) 154, 418
FatI CATG 1 cut(s) 407
FauI CCCGC 2 cut(s) 394, 885
Fnu4HI GCNGC 4 cut(s) 212, 215, 342, 890
FokI GGATG 6 cut(s) 269, 299, 344, 359, 428, 758
Fsp4HI GCNGC 4 cut(s) 212, 215, 342, 890
FspAI RTGCGCAY 1 cut(s) 518
FspBI CTAG 1 cut(s) 579
FspI TGCGCA 1 cut(s) 518
GlaI GCGC 1 cut(s) 518
GluI GCNGC 4 cut(s) 212, 215, 342, 890
GsaI CCCAGC 1 cut(s) 165
GsuI CTGGAG 1 cut(s) 642
HapII CCGG 1 cut(s) 937
HhaI GCGC 1 cut(s) 519
Hin1II CATG 1 cut(s) 411
Hin6I GCGC 1 cut(s) 517
HinP1I GCGC 1 cut(s) 517
HindIII AAGCTT 3 cut(s) 119, 527, 570
HinfI GANTC 3 cut(s) 335, 682, 920
HpaII CCGG 1 cut(s) 937
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 1 cut(s) 313
Hpy188I TCNGA 6 cut(s) 202, 253, 453, 681, 766, 949
Hpy188III TCNNGA 3 cut(s) 30, 579, 644
Hpy8I GTNNAC 1 cut(s) 313
Hpy99I CGWCG 3 cut(s) 272, 275, 278
HpyAV CCTTC 1 cut(s) 111
HpyCH4III ACNGT 2 cut(s) 19, 360
HpyCH4IV ACGT 3 cut(s) 276, 837, 928
HpyCH4V TGCA 5 cut(s) 53, 78, 181, 749, 819
HpyF10VI GCNNNNNNNGC 4 cut(s) 211, 350, 614, 898
HpyF3I CTNAG 4 cut(s) 207, 438, 630, 738
HpySE526I ACGT 3 cut(s) 276, 837, 928
Hsp92II CATG 1 cut(s) 411
HspAI GCGC 1 cut(s) 517
Kzo9I GATC 3 cut(s) 620, 697, 709
LmnI GCTCC 2 cut(s) 202, 551
Lsp1109I GCAGC 3 cut(s) 223, 226, 901
LweI GCATC 3 cut(s) 314, 736, 828
MaeI CTAG 1 cut(s) 579
MaeII ACGT 3 cut(s) 276, 837, 928
MaeIII GTNAC 2 cut(s) 464, 733
MalI GATC 3 cut(s) 622, 699, 711
MboI GATC 3 cut(s) 620, 697, 709
MboII GAAGA 3 cut(s) 254, 275, 572
MluCI AATT 4 cut(s) 5, 25, 390, 499
MlyI GAGTC 1 cut(s) 344
MmeI TCCRAC 1 cut(s) 118
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 5 cut(s) 11, 486, 846, 879, 955
MslI CAYNNNNRTG 1 cut(s) 512
MspA1I CMGCKG 1 cut(s) 892
MspI CCGG 1 cut(s) 937
MspR9I CCNGG 1 cut(s) 660
MvaI CCWGG 1 cut(s) 660
MwoI GCNNNNNNNGC 4 cut(s) 211, 350, 614, 898
NdeII GATC 3 cut(s) 620, 697, 709
NlaIII CATG 1 cut(s) 411
NlaIV GGNNCC 2 cut(s) 204, 547
NmuCI GTSAC 1 cut(s) 464
NsbI TGCGCA 1 cut(s) 518
NspV TTCGAA 1 cut(s) 533
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 2 cut(s) 682, 920
PkrI GCNGC 4 cut(s) 213, 216, 343, 891
PleI GAGTC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 343
PshBI ATTAAT 1 cut(s) 846
Psp6I CCWGG 1 cut(s) 658
PspFI CCCAGC 1 cut(s) 161
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 2 cut(s) 204, 547
PstI CTGCAG 1 cut(s) 183
PstNI CAGNNNCTG 1 cut(s) 629
RsaI GTAC 2 cut(s) 143, 627
RsaNI GTAC 2 cut(s) 142, 626
RseI CAYNNNNRTG 1 cut(s) 512
SaqAI TTAA 5 cut(s) 11, 486, 846, 879, 955
SatI GCNGC 4 cut(s) 212, 215, 342, 890
Sau3AI GATC 3 cut(s) 620, 697, 709
SchI GAGTC 1 cut(s) 344
ScrFI CCNGG 1 cut(s) 660
SfaNI GCATC 3 cut(s) 314, 736, 828
SfcI CTRYAG 2 cut(s) 179, 586
SfuI TTCGAA 1 cut(s) 533
SmiMI CAYNNNNRTG 1 cut(s) 512
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
Sse9I AATT 4 cut(s) 5, 25, 390, 499
SsiI CCGC 5 cut(s) 341, 344, 378, 401, 892
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 658
TaaI ACNGT 2 cut(s) 19, 360
TaiI ACGT 3 cut(s) 279, 840, 931
TaqI TCGA 3 cut(s) 533, 619, 712
TasI AATT 4 cut(s) 5, 25, 390, 499
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 2 cut(s) 682, 920
Tru1I TTAA 5 cut(s) 11, 486, 846, 879, 955
Tru9I TTAA 5 cut(s) 11, 486, 846, 879, 955
TscAI CASTG 2 cut(s) 183, 315
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 3 cut(s) 211, 214, 889
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 3 cut(s) 276, 432, 747
TspGWI ACGGA 1 cut(s) 758
TspRI CASTG 2 cut(s) 183, 315
VspI ATTAAT 1 cut(s) 846
XapI RAATTY 3 cut(s) 25, 390, 499
XbaI TCTAGA 1 cut(s) 578
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.