Rh1AG050100

F-actin-capping proteins bind in a Ca(2 )-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0021649
Physical Location & Seq
Forward (+)
8405838 .. 8407511
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG050100.1

Sequence Viewer

Length: 465 bp
ATGTGGGTGAAGCTTATCCAAAAGGAGTCTGCTCAGTGTATTGTGGGAATGGTAAAGATGCAGAGGGCCCAGGATCTGACTTTGAGCTCAAATGGTAGTTGGCGTTCAGTGTGGAGCATTGAGTTCAAAGATGAAGTACAAATGCTGGAATTAAAAGGCAAACTGCAGGTGGGTGCCCACTATTTTGAGAAGGGGAATGTGCAGTTAGATGCAAAACATGAATGCAAAGATTCAACAATTTTTCAGTCCTCTGAAGATTCTGCAATTACCATAGGCAACATTATAAGGCAACATGAGGCAGAATACTTGGCATCTCTTGAGGCATCCTATTCAAACTTGCCCGATACCACTTTTAAGGATCTTCGTAGAAAGCTTCCAGTTACTCGCACCCTATTTCCATGGCAGAATACTTTACAGTTTAGCTTGACAAGAGATATCACAAAAGAACTTGGCATTGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
F-actin_cap_A

Protein Analysis

154

Amino Acids

17.64

Weight (kDa)

7.76

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-actin_cap_A PF01267 31 - 145 2.4e-36 F-actin capping protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021649)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002050.1_g000003 Rmu_sc0002669.1_g000001 Rmu_sc0002882.1_g000017 Rmu_sc0008565.1_g000017
rosa_samantha Rh1AG050100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 284
AarI CACCTGC 1 cut(s) 157
Acc36I ACCTGC 1 cut(s) 157
AccB1I GGYRCC 1 cut(s) 173
AclWI GGATC 2 cut(s) 81, 366
AcuI CTGAAG 1 cut(s) 273
AfaI GTAC 1 cut(s) 138
AgsI TTSAA 3 cut(s) 127, 234, 333
AjnI CCWGG 1 cut(s) 69
AjuI GAANNNNNNNTTGG 1 cut(s) 438
AluBI AGCT 4 cut(s) 13, 87, 373, 423
AluI AGCT 4 cut(s) 13, 87, 373, 423
Alw21I GWGCWC 1 cut(s) 89
AlwI GGATC 2 cut(s) 81, 366
AlwNI CAGNNNCTG 1 cut(s) 76
AoxI GGCC 1 cut(s) 66
ApaI GGGCCC 1 cut(s) 70
AspS9I GGNCC 2 cut(s) 66, 67
AsuHPI GGTGA 1 cut(s) 19
BaeGI GKGCMC 2 cut(s) 70, 178
BanI GGYRCC 1 cut(s) 173
BanII GRGCYC 2 cut(s) 70, 89
Bbv12I GWGCWC 1 cut(s) 89
BciT130I CCWGG 1 cut(s) 71
BfmI CTRYAG 1 cut(s) 164
BfuAI ACCTGC 1 cut(s) 157
Bme1390I CCNGG 1 cut(s) 71
BmgT120I GGNCC 2 cut(s) 66, 67
BmiI GGNNCC 2 cut(s) 68, 175
BmrFI CCNGG 1 cut(s) 71
BmsI GCATC 4 cut(s) 48, 199, 320, 332
BpuEI CTTGAG 1 cut(s) 338
BsaJI CCNNGG 2 cut(s) 69, 398
Bse1I ACTGG 1 cut(s) 377
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 2 cut(s) 69, 398
BseGI GGATG 1 cut(s) 323
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 377
BseSI GKGCMC 2 cut(s) 70, 178
BsgI GTGCAG 1 cut(s) 221
BshFI GGCC 1 cut(s) 68
BshNI GGYRCC 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 89
BsmI GAATGC 1 cut(s) 227
BsnI GGCC 1 cut(s) 68
Bsp120I GGGCCC 1 cut(s) 66
Bsp1286I GDGCHC 3 cut(s) 70, 89, 178
Bsp143I GATC 2 cut(s) 73, 358
Bsp19I CCATGG 1 cut(s) 398
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 68, 175
BspMAI CTGCAG 1 cut(s) 168
BspMI ACCTGC 1 cut(s) 157
BspPI GGATC 2 cut(s) 81, 366
BspT107I GGYRCC 1 cut(s) 173
BsrI ACTGG 1 cut(s) 377
BssECI CCNNGG 2 cut(s) 69, 398
BssMI GATC 2 cut(s) 73, 358
BssT1I CCWWGG 1 cut(s) 398
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 1 cut(s) 417
BstDEI CTNAG 1 cut(s) 33
BstDSI CCRYGG 1 cut(s) 398
BstF5I GGATG 1 cut(s) 323
BstKTI GATC 2 cut(s) 76, 361
BstMBI GATC 2 cut(s) 73, 358
BstNI CCWGG 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 69
BstSFI CTRYAG 1 cut(s) 164
BstSLI GKGCMC 2 cut(s) 70, 178
BstX2I RGATCY 2 cut(s) 73, 358
BstYI RGATCY 2 cut(s) 73, 358
BsuRI GGCC 1 cut(s) 68
BtgI CCRYGG 1 cut(s) 398
BtsCI GGATG 1 cut(s) 323
BtsIMutI CAGTG 2 cut(s) 41, 114
BveI ACCTGC 1 cut(s) 157
CaiI CAGNNNCTG 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 66, 67
Csp6I GTAC 1 cut(s) 137
CviAII CATG 3 cut(s) 218, 293, 399
CviJI RGCY 5 cut(s) 13, 68, 87, 373, 423
CviKI_1 RGCY 5 cut(s) 13, 68, 87, 373, 423
CviQI GTAC 1 cut(s) 137
DdeI CTNAG 1 cut(s) 33
DpnI GATC 2 cut(s) 75, 360
DpnII GATC 2 cut(s) 73, 358
Ecl136II GAGCTC 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 398
Eco24I GRGCYC 2 cut(s) 70, 89
Eco32I GATATC 1 cut(s) 436
Eco53kI GAGCTC 1 cut(s) 87
Eco57I CTGAAG 1 cut(s) 273
EcoICRI GAGCTC 1 cut(s) 87
EcoO109I RGGNCCY 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 69
EcoRV GATATC 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 398
EcoT38I GRGCYC 2 cut(s) 70, 89
ErhI CCWWGG 1 cut(s) 398
FaeI CATG 3 cut(s) 221, 296, 402
FaiI YATR 5 cut(s) 219, 272, 284, 294, 400
FatI CATG 3 cut(s) 217, 292, 398
FokI GGATG 1 cut(s) 310
FriOI GRGCYC 2 cut(s) 70, 89
HaeIII GGCC 1 cut(s) 68
Hin1II CATG 3 cut(s) 221, 296, 402
HindIII AAGCTT 2 cut(s) 11, 371
HinfI GANTC 3 cut(s) 26, 230, 257
HphI GGTGA 1 cut(s) 19
Hpy188I TCNGA 2 cut(s) 78, 253
Hpy188III TCNNGA 1 cut(s) 317
HpyAV CCTTC 1 cut(s) 184
HpyCH4III ACNGT 1 cut(s) 417
HpyCH4V TGCA 6 cut(s) 61, 166, 202, 212, 225, 263
HpyF3I CTNAG 1 cut(s) 33
Hsp92II CATG 3 cut(s) 221, 296, 402
Kzo9I GATC 2 cut(s) 73, 358
LmnI GCTCC 1 cut(s) 114
LpnPI CCDG 5 cut(s) 56, 83, 131, 152, 390
LweI GCATC 4 cut(s) 48, 199, 320, 332
MaeIII GTNAC 1 cut(s) 379
MalI GATC 2 cut(s) 75, 360
MboI GATC 2 cut(s) 73, 358
MboII GAAGA 2 cut(s) 266, 353
MflI RGATCY 2 cut(s) 73, 358
MhlI GDGCHC 3 cut(s) 70, 89, 178
MluCI AATT 3 cut(s) 149, 237, 264
MlyI GAGTC 1 cut(s) 35
MnlI CCTC 4 cut(s) 57, 259, 289, 313
MseI TTAA 2 cut(s) 152, 354
MspR9I CCNGG 1 cut(s) 71
Mva1269I GAATGC 1 cut(s) 227
MvaI CCWGG 1 cut(s) 71
NcoI CCATGG 1 cut(s) 398
NdeII GATC 2 cut(s) 73, 358
NlaIII CATG 3 cut(s) 221, 296, 402
NlaIV GGNNCC 2 cut(s) 68, 175
PaqCI CACCTGC 1 cut(s) 157
PctI GAATGC 1 cut(s) 227
PfeI GAWTC 2 cut(s) 230, 257
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
PsiI TTATAA 1 cut(s) 284
Psp124BI GAGCTC 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 69
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 2 cut(s) 68, 175
PspOMI GGGCCC 1 cut(s) 66
PspPI GGNCC 2 cut(s) 66, 67
PsrI GAACNNNNNNTAC 2 cut(s) 88, 120
PstI CTGCAG 1 cut(s) 168
PstNI CAGNNNCTG 1 cut(s) 76
PsuI RGATCY 2 cut(s) 73, 358
RsaI GTAC 1 cut(s) 138
RsaNI GTAC 1 cut(s) 137
SacI GAGCTC 1 cut(s) 89
SaqAI TTAA 2 cut(s) 152, 354
Sau3AI GATC 2 cut(s) 73, 358
Sau96I GGNCC 2 cut(s) 66, 67
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 1 cut(s) 71
SduI GDGCHC 3 cut(s) 70, 89, 178
SetI ASST 5 cut(s) 15, 89, 171, 375, 425
SfaNI GCATC 4 cut(s) 48, 199, 320, 332
SfcI CTRYAG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 3 cut(s) 149, 237, 264
SstI GAGCTC 1 cut(s) 89
StyD4I CCNGG 1 cut(s) 69
StyI CCWWGG 1 cut(s) 398
TaaI ACNGT 1 cut(s) 417
TasI AATT 3 cut(s) 149, 237, 264
TatI WGTACW 1 cut(s) 136
TfiI GAWTC 2 cut(s) 230, 257
Tru1I TTAA 2 cut(s) 152, 354
Tru9I TTAA 2 cut(s) 152, 354
TscAI CASTG 2 cut(s) 41, 114
TspDTI ATGAA 2 cut(s) 147, 234
TspRI CASTG 2 cut(s) 41, 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.