Rh1CG120000
ERF Family

Performs the first committed step in the biosynthesis of isoprene-containing compounds such as sterols and terpenoids

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0031201
Physical Location & Seq
Forward (+)
25981720 .. 25982221
502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG120000.1

Sequence Viewer

Length: 375 bp
ATGAATTCTCGCACGCGCGAGCGCGGCCCATGCGCGTGCAGAAAGGCTAGTACATTTGATGTGGGCCCGAATGCGGTTATGATTGCACGTGATAGAAAAACTGCTTCCCTTTTGCTTCAGAAGCTTCTTTACACTTTCCCTCCAAGTTCTGATGCGGATTTGAACAGTTTTGTTCTTGGTGATAAGTCAATTTTAAAAGATGCTGGTATTAATTCCCCGGAGGATATCGCGACTTTGCCCCCACCTCCAGAAATCAAGGGCCAGCAGTTCAAAGGAAATGTTAGTTGCTTTATTTGCACAAGACCTGGGAGAGGTCCAGTGGTGTTGTCAGATGAAACTCATCTTCTCAACCCTGACACTGGGCTGCCGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004163 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006066 GO:0006084 GO:0006139 GO:0006163 GO:0006464 GO:0006486 GO:0006487 GO:0006488 GO:0006489 GO:0006490 GO:0006629 GO:0006637 GO:0006644 GO:0006694 GO:0006695 GO:0006720 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008202 GO:0008203 GO:0008284 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009117 GO:0009150 GO:0009240 GO:0009259 GO:0009889 GO:0009987 GO:0016125 GO:0016126 GO:0016829 GO:0016830 GO:0016831 GO:0019216 GO:0019218 GO:0019222 GO:0019287 GO:0019538 GO:0019637 GO:0019693 GO:0030544 GO:0031072 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0034645 GO:0035383 GO:0036211 GO:0042127 GO:0042579 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0045540 GO:0046165 GO:0046465 GO:0046483 GO:0046490 GO:0046890 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050810 GO:0051186 GO:0055086 GO:0062012 GO:0065007 GO:0070085 GO:0071704 GO:0072521 GO:0080090 GO:0090181 GO:0090407 GO:0106118 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617 GO:1902652 GO:1902653 GO:1902930
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
GHMP_kinases_N

Protein Analysis

124

Amino Acids

13.39

Weight (kDa)

8.78

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MDD_C PF18376 14 - 108 1.6e-07 Mevalonate 5-diphosphate decarboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031201)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002307.1_g000015
rosa_samantha Rh1CG120000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 16, 18, 24, 35, 230
AciI CCGC 3 cut(s) 24, 74, 155
AcsI RAATTY 1 cut(s) 4
AcuI CTGAAG 1 cut(s) 101
AcvI CACGTG 1 cut(s) 89
AfaI GTAC 1 cut(s) 52
AfiI CCNNNNNNNGG 3 cut(s) 73, 311, 359
AgsI TTSAA 2 cut(s) 163, 271
AjnI CCWGG 1 cut(s) 304
AluBI AGCT 1 cut(s) 124
AluI AGCT 1 cut(s) 124
AoxI GGCC 3 cut(s) 25, 64, 259
ApaI GGGCCC 1 cut(s) 68
ApeKI GCWGC 1 cut(s) 364
ApoI RAATTY 1 cut(s) 4
AseI ATTAAT 1 cut(s) 210
AspLEI GCGC 3 cut(s) 18, 24, 35
AspS9I GGNCC 5 cut(s) 26, 64, 65, 259, 314
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 1 cut(s) 191
AvaII GGWCC 1 cut(s) 314
BaeGI GKGCMC 1 cut(s) 68
BanII GRGCYC 1 cut(s) 68
BarI GAAGNNNNNNTAC 2 cut(s) 113, 145
BbrPI CACGTG 1 cut(s) 89
BbvI GCAGC 1 cut(s) 351
BciT130I CCWGG 1 cut(s) 306
BcnI CCSGG 1 cut(s) 218
BfaI CTAG 1 cut(s) 48
BisI GCNGC 2 cut(s) 25, 365
BlsI GCNGC 2 cut(s) 26, 366
Bme1390I CCNGG 2 cut(s) 218, 306
Bme18I GGWCC 1 cut(s) 314
BmgT120I GGNCC 5 cut(s) 26, 64, 65, 259, 314
BmiI GGNNCC 1 cut(s) 66
BmrFI CCNGG 2 cut(s) 218, 306
BmrI ACTGGG 1 cut(s) 369
BmsI GCATC 2 cut(s) 142, 190
BmuI ACTGGG 1 cut(s) 369
BpmI CTGGAG 1 cut(s) 231
BpuMI CCSGG 1 cut(s) 218
BsaAI YACGTR 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 216, 305
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 311, 359
Bse1I ACTGG 2 cut(s) 317, 364
BseBI CCWGG 1 cut(s) 306
BseDI CCNNGG 2 cut(s) 216, 305
BseLI CCNNNNNNNGG 3 cut(s) 73, 311, 359
BseNI ACTGG 2 cut(s) 317, 364
BseSI GKGCMC 1 cut(s) 68
BseXI GCAGC 1 cut(s) 351
BsgI GTGCAG 1 cut(s) 58
Bsh1236I CGCG 5 cut(s) 16, 18, 24, 35, 230
BshFI GGCC 3 cut(s) 27, 66, 261
BsiSI CCGG 1 cut(s) 218
BslI CCNNNNNNNGG 3 cut(s) 73, 311, 359
BsmI GAATGC 1 cut(s) 76
BsnI GGCC 3 cut(s) 27, 66, 261
Bsp120I GGGCCC 1 cut(s) 64
Bsp1286I GDGCHC 1 cut(s) 68
Bsp68I TCGCGA 1 cut(s) 230
BspACI CCGC 3 cut(s) 24, 74, 155
BspANI GGCC 3 cut(s) 27, 66, 261
BspFNI CGCG 5 cut(s) 16, 18, 24, 35, 230
BspLI GGNNCC 1 cut(s) 66
BsrI ACTGG 2 cut(s) 317, 364
BssECI CCNNGG 2 cut(s) 216, 305
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 167
BstBAI YACGTR 1 cut(s) 89
BstC8I GCNNGC 4 cut(s) 14, 20, 37, 263
BstENI CCTNNNNNAGG 1 cut(s) 309
BstFNI CGCG 5 cut(s) 16, 18, 24, 35, 230
BstHHI GCGC 3 cut(s) 18, 24, 35
BstMWI GCNNNNNNNGC 4 cut(s) 24, 30, 121, 294
BstNI CCWGG 1 cut(s) 306
BstSCI CCNGG 2 cut(s) 216, 304
BstSLI GKGCMC 1 cut(s) 68
BstUI CGCG 5 cut(s) 16, 18, 24, 35, 230
BstV1I GCAGC 1 cut(s) 351
BsuRI GGCC 3 cut(s) 27, 66, 261
BtsIMutI CAGTG 2 cut(s) 324, 357
BtuMI TCGCGA 1 cut(s) 230
Cac8I GCNNGC 4 cut(s) 14, 20, 37, 263
CfoI GCGC 3 cut(s) 18, 24, 35
Cfr13I GGNCC 5 cut(s) 26, 64, 65, 259, 314
Csp6I GTAC 1 cut(s) 51
CviAII CATG 1 cut(s) 30
CviJI RGCY 6 cut(s) 27, 47, 66, 124, 261, 364
CviKI_1 RGCY 6 cut(s) 27, 47, 66, 124, 261, 364
CviQI GTAC 1 cut(s) 51
DraI TTTAAA 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 68
Eco32I GATATC 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 101
Eco72I CACGTG 1 cut(s) 89
EcoNI CCTNNNNNAGG 1 cut(s) 309
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 304
EcoRV GATATC 1 cut(s) 226
EcoT38I GRGCYC 1 cut(s) 68
FaeI CATG 1 cut(s) 33
FaiI YATR 2 cut(s) 31, 80
FatI CATG 1 cut(s) 29
Fnu4HI GCNGC 2 cut(s) 25, 365
FriOI GRGCYC 1 cut(s) 68
Fsp4HI GCNGC 2 cut(s) 25, 365
FspBI CTAG 1 cut(s) 48
GlaI GCGC 3 cut(s) 17, 23, 34
GluI GCNGC 2 cut(s) 25, 365
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 3 cut(s) 27, 66, 261
HapII CCGG 1 cut(s) 218
HhaI GCGC 3 cut(s) 18, 24, 35
Hin1II CATG 1 cut(s) 33
Hin6I GCGC 3 cut(s) 16, 22, 33
HinP1I GCGC 3 cut(s) 16, 22, 33
HindIII AAGCTT 1 cut(s) 122
HpaII CCGG 1 cut(s) 218
HphI GGTGA 1 cut(s) 191
Hpy188I TCNGA 3 cut(s) 120, 151, 331
Hpy188III TCNNGA 2 cut(s) 229, 248
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4IV ACGT 1 cut(s) 88
HpyCH4V TGCA 3 cut(s) 39, 86, 297
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 30, 121, 294
HpySE526I ACGT 1 cut(s) 88
Hsp92II CATG 1 cut(s) 33
HspAI GCGC 3 cut(s) 16, 22, 33
LpnPI CCDG 9 cut(s) 189, 231, 261, 275, 291, 318, 330, 345, 366
Lsp1109I GCAGC 1 cut(s) 351
LweI GCATC 2 cut(s) 142, 190
MaeI CTAG 1 cut(s) 48
MaeII ACGT 1 cut(s) 88
MboII GAAGA 1 cut(s) 335
MhlI GDGCHC 1 cut(s) 68
MluCI AATT 3 cut(s) 4, 189, 211
MnlI CCTC 4 cut(s) 150, 214, 255, 305
MseI TTAA 2 cut(s) 194, 210
MslI CAYNNNNRTG 1 cut(s) 34
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 2 cut(s) 218, 306
Mva1269I GAATGC 1 cut(s) 76
MvaI CCWGG 1 cut(s) 306
MvnI CGCG 5 cut(s) 16, 18, 24, 35, 230
MwoI GCNNNNNNNGC 4 cut(s) 24, 30, 121, 294
NciI CCSGG 1 cut(s) 218
NlaIII CATG 1 cut(s) 33
NlaIV GGNNCC 1 cut(s) 66
NruI TCGCGA 1 cut(s) 230
PctI GAATGC 1 cut(s) 76
PkrI GCNGC 2 cut(s) 26, 366
PmaCI CACGTG 1 cut(s) 89
PmlI CACGTG 1 cut(s) 89
Ppu21I YACGTR 1 cut(s) 89
PshBI ATTAAT 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 304
PspCI CACGTG 1 cut(s) 89
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 1 cut(s) 66
PspOMI GGGCCC 1 cut(s) 64
PspPI GGNCC 5 cut(s) 26, 64, 65, 259, 314
RruI TCGCGA 1 cut(s) 230
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
RseI CAYNNNNRTG 1 cut(s) 34
SaqAI TTAA 2 cut(s) 194, 210
SatI GCNGC 2 cut(s) 25, 365
Sau96I GGNCC 5 cut(s) 26, 64, 65, 259, 314
ScrFI CCNGG 2 cut(s) 218, 306
SduI GDGCHC 1 cut(s) 68
SetI ASST 5 cut(s) 91, 126, 247, 307, 316
SfaNI GCATC 2 cut(s) 142, 190
SinI GGWCC 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 34
Sse9I AATT 3 cut(s) 4, 189, 211
SsiI CCGC 3 cut(s) 24, 74, 155
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 2 cut(s) 216, 304
TaaI ACNGT 1 cut(s) 167
TaiI ACGT 1 cut(s) 91
TasI AATT 3 cut(s) 4, 189, 211
TatI WGTACW 1 cut(s) 50
TauI GCSGC 1 cut(s) 27
Tru1I TTAA 2 cut(s) 194, 210
Tru9I TTAA 2 cut(s) 194, 210
TscAI CASTG 2 cut(s) 324, 364
TseI GCWGC 1 cut(s) 364
TspDTI ATGAA 2 cut(s) 17, 348
TspRI CASTG 2 cut(s) 324, 364
VpaK11BI GGWCC 1 cut(s) 314
VspI ATTAAT 1 cut(s) 210
XagI CCTNNNNNAGG 1 cut(s) 309
XapI RAATTY 1 cut(s) 4
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.