Rh2AG341600

Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0029744
Physical Location & Seq
Reverse (-)
49923405 .. 49941186
17782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG341600.1

Sequence Viewer

Length: 1047 bp
ATGGGGAATTTGAGAAGTGGGGATCTTGATGAGGTGATTGTTGTGTTTGAAAGTCTGGATAAGCAGGATTTTCGAGGAGAGGGAACAAAGGAGGTGGGTGGAGTGAGCAATGAGGTCAGGGTTTTTAGGTCTTTCATGGGTGGAAAAAGAAGAAGAGAAGTAGGAGAGCCTTCGTGGTTTAGGTATCCGCGTACCGAGAGCACAGCCTATCCTTCTTCTTTTGACTTTAGAGGCACGCTTGGTGCACAAGTAAATAACCCCGTATGGGGTAGTTATGTACAGAGGCTGCTCACTGATGGTTATCAAAAACCACGATCTGGGACAGATGCAGGTGATCATCCTCCTGTTACTCCATTGAGGTCTGCCACTGAGGATATGCTGGGAAATGATGCGTGGAGATTATACGAGTATGTCTGTAAACACTTCATAGGGACTGTATCTCCTGACTGCAAGTATGTAAGAACAAAGGCAGAATTTTCAATTGGTGGAGAGTTCTTCCATTGTGTAGGACAACATGTTTTGGTGAAGGGATTTACTTCTATTATGCCATGGTTAGCAGTAAATGAGAAAAATCTTCCTCAATTTACAGAAGGCGAGAAAATAGAAATTGCCAGAGTGGACCTTCATGAGGGTATGACCTCACCTCCAGAATATTTAAGTGAGAGTGAGCTAATTTCGCTTATGGAGAAGAATGGAATCGGCACGGATGCATCAATATCTGTTCATATTAACAACATATGTGAGCGCAATTATGTGCAGGTACAAGCTGGCAGGAAGTTGGTTCCAACTACCTTAGGTACTACTCTAATAAGAGGCTATCAATGTATTGATCCAGATCTCTGTTTGCCAGATATCCGCAGTTTTATTGAGCAGCAGATTACTCTTGTTGCTAAGGGTCAAGCAGATCTGAATGTTTTTATGAATAGTTGGTTTTCAATGTCCTTGAACTCAATCTCCCCAATAGCAAGAACAATAGGAAAGAACAGGACCCATATATATATATATATATATATATATCATTGTGTTTCATTGAACAAAGCAAGTTAG

Protein Analysis

348

Amino Acids

39.13

Weight (kDa)

6.27

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_bac PF01131 62 - 307 3.3e-58 DNA topoisomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029744)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0108981
rosa_samantha Rh2AG341600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 320
Acc36I ACCTGC 2 cut(s) 320, 748
AccB7I CCANNNNNTGG 1 cut(s) 317
AccII CGCG 1 cut(s) 190
AciI CCGC 2 cut(s) 188, 856
AclWI GGATC 2 cut(s) 30, 824
AcsI RAATTY 2 cut(s) 7, 473
AfaI GTAC 4 cut(s) 193, 279, 762, 799
AfiI CCNNNNNNNGG 4 cut(s) 265, 266, 317, 628
AflIII ACRYGT 1 cut(s) 514
AgsI TTSAA 5 cut(s) 50, 480, 936, 946, 1033
AjuI GAANNNNNNNTTGG 2 cut(s) 465, 497
AloI GAACNNNNNNTCC 2 cut(s) 938, 970
AluBI AGCT 2 cut(s) 670, 767
AluI AGCT 2 cut(s) 670, 767
Alw21I GWGCWC 2 cut(s) 203, 247
Alw44I GTGCAC 1 cut(s) 243
AlwI GGATC 2 cut(s) 30, 824
AlwNI CAGNNNCTG 1 cut(s) 286
ApaLI GTGCAC 1 cut(s) 243
ApeKI GCWGC 2 cut(s) 286, 871
ApoI RAATTY 2 cut(s) 7, 473
AspLEI GCGC 1 cut(s) 747
AspS9I GGNCC 2 cut(s) 619, 987
AsuHPI GGTGA 4 cut(s) 46, 344, 535, 633
AvaII GGWCC 2 cut(s) 619, 987
AxyI CCTNAGG 1 cut(s) 793
BaeGI GKGCMC 1 cut(s) 247
Bbv12I GWGCWC 2 cut(s) 203, 247
BbvI GCAGC 2 cut(s) 273, 883
BccI CCATC 1 cut(s) 290
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BciVI GTATCC 1 cut(s) 195
BclI TGATCA 1 cut(s) 334
BfuAI ACCTGC 2 cut(s) 320, 748
BfuI GTATCC 1 cut(s) 195
BglII AGATCT 2 cut(s) 835, 904
BisI GCNGC 2 cut(s) 287, 872
BlsI GCNGC 2 cut(s) 288, 873
Bme18I GGWCC 2 cut(s) 619, 987
BmgT120I GGNCC 2 cut(s) 619, 987
BmiI GGNNCC 2 cut(s) 783, 989
BmsI GCATC 4 cut(s) 316, 379, 697, 719
BpmI CTGGAG 1 cut(s) 630
Bpu10I CCTNAGC 1 cut(s) 891
BsaBI GATNNNNATC 2 cut(s) 300, 834
BsaJI CCNNGG 1 cut(s) 548
BsaXI ACNNNNNCTCC 6 cut(s) 424, 454, 628, 658, 938, 968
Bsc4I CCNNNNNNNGG 4 cut(s) 265, 266, 317, 628
Bse21I CCTNAGG 1 cut(s) 793
Bse3DI GCAATG 1 cut(s) 115
Bse8I GATNNNNATC 2 cut(s) 300, 834
BseDI CCNNGG 1 cut(s) 548
BseGI GGATG 2 cut(s) 337, 712
BseJI GATNNNNATC 2 cut(s) 300, 834
BseLI CCNNNNNNNGG 4 cut(s) 265, 266, 317, 628
BseMI GCAATG 1 cut(s) 115
BseMII CTCAG 1 cut(s) 360
BseRI GAGGAG 1 cut(s) 90
BseSI GKGCMC 1 cut(s) 247
BseXI GCAGC 2 cut(s) 273, 883
BseYI CCCAGC 1 cut(s) 379
BsgI GTGCAG 1 cut(s) 776
Bsh1236I CGCG 1 cut(s) 190
BsiHKAI GWGCWC 2 cut(s) 203, 247
BslFI GGGAC 2 cut(s) 334, 445
BslI CCNNNNNNNGG 4 cut(s) 265, 266, 317, 628
BsmFI GGGAC 2 cut(s) 334, 445
Bsp1286I GDGCHC 2 cut(s) 203, 247
Bsp1407I TGTACA 1 cut(s) 277
Bsp143I GATC 6 cut(s) 22, 314, 334, 829, 835, 904
Bsp19I CCATGG 1 cut(s) 548
BspACI CCGC 2 cut(s) 188, 856
BspCNI CTCAG 1 cut(s) 361
BspFNI CGCG 1 cut(s) 190
BspHI TCATGA 1 cut(s) 625
BspLI GGNNCC 2 cut(s) 783, 989
BspMI ACCTGC 2 cut(s) 320, 748
BspPI GGATC 2 cut(s) 30, 824
BsrDI GCAATG 1 cut(s) 115
BsrGI TGTACA 1 cut(s) 277
BssECI CCNNGG 1 cut(s) 548
BssMI GATC 6 cut(s) 22, 314, 334, 829, 835, 904
BssT1I CCWWGG 1 cut(s) 548
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 148
BstAUI TGTACA 1 cut(s) 277
BstC8I GCNNGC 2 cut(s) 236, 769
BstDEI CTNAG 3 cut(s) 369, 793, 891
BstDSI CCRYGG 1 cut(s) 548
BstENI CCTNNNNNAGG 1 cut(s) 626
BstF5I GGATG 2 cut(s) 337, 712
BstFNI CGCG 1 cut(s) 190
BstHHI GCGC 1 cut(s) 747
BstKTI GATC 6 cut(s) 25, 317, 337, 832, 838, 907
BstMBI GATC 6 cut(s) 22, 314, 334, 829, 835, 904
BstMWI GCNNNNNNNGC 1 cut(s) 676
BstNSI RCATGY 1 cut(s) 518
BstSLI GKGCMC 1 cut(s) 247
BstUI CGCG 1 cut(s) 190
BstV1I GCAGC 2 cut(s) 273, 883
BstX2I RGATCY 3 cut(s) 22, 835, 904
BstYI RGATCY 3 cut(s) 22, 835, 904
Bsu36I CCTNAGG 1 cut(s) 793
BsuI GTATCC 1 cut(s) 195
BtgI CCRYGG 1 cut(s) 548
BtsCI GGATG 2 cut(s) 337, 712
BtsIMutI CAGTG 2 cut(s) 291, 366
BveI ACCTGC 2 cut(s) 320, 748
Cac8I GCNNGC 2 cut(s) 236, 769
CaiI CAGNNNCTG 1 cut(s) 286
CciI TCATGA 1 cut(s) 625
CfoI GCGC 1 cut(s) 747
Cfr13I GGNCC 2 cut(s) 619, 987
Csp6I GTAC 4 cut(s) 192, 278, 761, 798
CspCI CAANNNNNGTGG 2 cut(s) 75, 110
CviAII CATG 4 cut(s) 136, 515, 549, 626
CviJI RGCY 6 cut(s) 169, 206, 286, 670, 767, 816
CviKI_1 RGCY 6 cut(s) 169, 206, 286, 670, 767, 816
CviQI GTAC 4 cut(s) 192, 278, 761, 798
DdeI CTNAG 3 cut(s) 369, 793, 891
DpnI GATC 6 cut(s) 24, 316, 336, 831, 837, 906
DpnII GATC 6 cut(s) 22, 314, 334, 829, 835, 904
Eam1104I CTCTTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 548
Eco32I GATATC 1 cut(s) 853
Eco47I GGWCC 2 cut(s) 619, 987
Eco81I CCTNAGG 1 cut(s) 793
EcoNI CCTNNNNNAGG 1 cut(s) 626
EcoO109I RGGNCCY 1 cut(s) 987
EcoRV GATATC 1 cut(s) 853
EcoT14I CCWWGG 1 cut(s) 548
EcoT22I ATGCAT 1 cut(s) 712
ErhI CCWWGG 1 cut(s) 548
FaeI CATG 4 cut(s) 139, 518, 552, 629
FaqI GGGAC 2 cut(s) 334, 445
FatI CATG 4 cut(s) 135, 514, 548, 625
FauNDI CATATG 1 cut(s) 737
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 287, 872
FokI GGATG 2 cut(s) 324, 719
Fsp4HI GCNGC 2 cut(s) 287, 872
GlaI GCGC 1 cut(s) 746
GluI GCNGC 2 cut(s) 287, 872
GsaI CCCAGC 1 cut(s) 383
GsuI CTGGAG 1 cut(s) 630
HhaI GCGC 1 cut(s) 747
Hin1II CATG 4 cut(s) 139, 518, 552, 629
Hin6I GCGC 1 cut(s) 745
HinP1I GCGC 1 cut(s) 745
HinfI GANTC 1 cut(s) 696
HphI GGTGA 4 cut(s) 46, 344, 535, 633
Hpy166II GTNNAC 3 cut(s) 245, 419, 619
Hpy188I TCNGA 1 cut(s) 909
Hpy188III TCNNGA 6 cut(s) 26, 56, 443, 626, 647, 833
Hpy8I GTNNAC 3 cut(s) 245, 419, 619
HpyAV CCTTC 5 cut(s) 180, 222, 520, 584, 632
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4V TGCA 5 cut(s) 245, 329, 450, 710, 757
HpyF10VI GCNNNNNNNGC 1 cut(s) 676
HpyF3I CTNAG 3 cut(s) 369, 793, 891
Hsp92II CATG 4 cut(s) 139, 518, 552, 629
HspAI GCGC 1 cut(s) 745
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 6 cut(s) 22, 314, 334, 829, 835, 904
Lsp1109I GCAGC 2 cut(s) 273, 883
LweI GCATC 4 cut(s) 316, 379, 697, 719
MaeIII GTNAC 1 cut(s) 346
MalI GATC 6 cut(s) 24, 316, 336, 831, 837, 906
MboI GATC 6 cut(s) 22, 314, 334, 829, 835, 904
MboII GAAGA 6 cut(s) 162, 165, 207, 487, 566, 700
MfeI CAATTG 1 cut(s) 480
MflI RGATCY 3 cut(s) 22, 835, 904
MhlI GDGCHC 2 cut(s) 203, 247
MluCI AATT 7 cut(s) 7, 473, 480, 581, 606, 672, 748
MmeI TCCRAC 1 cut(s) 809
Mph1103I ATGCAT 1 cut(s) 712
MseI TTAA 2 cut(s) 656, 729
MunI CAATTG 1 cut(s) 480
MvnI CGCG 1 cut(s) 190
MwoI GCNNNNNNNGC 1 cut(s) 676
NcoI CCATGG 1 cut(s) 548
NdeI CATATG 1 cut(s) 737
NdeII GATC 6 cut(s) 22, 314, 334, 829, 835, 904
NlaIII CATG 4 cut(s) 139, 518, 552, 629
NlaIV GGNNCC 2 cut(s) 783, 989
NsiI ATGCAT 1 cut(s) 712
NspI RCATGY 1 cut(s) 518
PagI TCATGA 1 cut(s) 625
PaqCI CACCTGC 1 cut(s) 320
PciI ACATGT 1 cut(s) 514
PfeI GAWTC 1 cut(s) 696
PflMI CCANNNNNTGG 1 cut(s) 317
PkrI GCNGC 2 cut(s) 288, 873
PpuMI RGGWCCY 1 cut(s) 987
PscI ACATGT 1 cut(s) 514
Psp5II RGGWCCY 1 cut(s) 987
PspFI CCCAGC 1 cut(s) 379
PspN4I GGNNCC 2 cut(s) 783, 989
PspPI GGNCC 2 cut(s) 619, 987
PspPPI RGGWCCY 1 cut(s) 987
PstNI CAGNNNCTG 1 cut(s) 286
PsuI RGATCY 3 cut(s) 22, 835, 904
RsaI GTAC 4 cut(s) 193, 279, 762, 799
RsaNI GTAC 4 cut(s) 192, 278, 761, 798
SaqAI TTAA 2 cut(s) 656, 729
SatI GCNGC 2 cut(s) 287, 872
Sau3AI GATC 6 cut(s) 22, 314, 334, 829, 835, 904
Sau96I GGNCC 2 cut(s) 619, 987
SduI GDGCHC 2 cut(s) 203, 247
SfaNI GCATC 4 cut(s) 316, 379, 697, 719
SinI GGWCC 2 cut(s) 619, 987
Sse9I AATT 7 cut(s) 7, 473, 480, 581, 606, 672, 748
SsiI CCGC 2 cut(s) 188, 856
SspI AATATT 1 cut(s) 653
StyI CCWWGG 1 cut(s) 548
TaaI ACNGT 1 cut(s) 436
TaqI TCGA 1 cut(s) 73
TasI AATT 7 cut(s) 7, 473, 480, 581, 606, 672, 748
TatI WGTACW 1 cut(s) 277
TfiI GAWTC 1 cut(s) 696
Tru1I TTAA 2 cut(s) 656, 729
Tru9I TTAA 2 cut(s) 656, 729
TscAI CASTG 2 cut(s) 298, 373
TseI GCWGC 2 cut(s) 286, 871
TspDTI ATGAA 6 cut(s) 124, 415, 614, 713, 935, 1017
TspGWI ACGGA 1 cut(s) 719
TspRI CASTG 2 cut(s) 298, 373
Van91I CCANNNNNTGG 1 cut(s) 317
VneI GTGCAC 1 cut(s) 243
VpaK11BI GGWCC 2 cut(s) 619, 987
XagI CCTNNNNNAGG 1 cut(s) 626
XapI RAATTY 2 cut(s) 7, 473
XceI RCATGY 1 cut(s) 518
Zsp2I ATGCAT 1 cut(s) 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.