Rh2DG175400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0033014
Physical Location & Seq
Forward (+)
15400008 .. 15400421
414 bp
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UTR
Exon/CDS
Intron
Rh2DG175400.1

Sequence Viewer

Length: 414 bp
ATGCAGAGCCAATGGGCTTTGGTCCCTCAGCCTGCATTCTGCTCCTTTCCTGATGGGATTGGGAAGCTGATCAAATTGGAAGTCTTGAGGCTAAAGTCTTGTACTGACTTAAAAACATTTCCGGCCTCAATTAAGGACCTTGAAAAGTTAAACTTTCTCGACATATCTGATTGTTTCAGCATTAAGGAGTTGCCTGACAACATTGGTGAAATAAGCACTTTAGAAAAGATCAATATGAGACAGTGCTCTAGAGTGCAAGATCTACCTCTATCAGTTATGGATCTTAAGAAACTGAAGGAGGTGATATGTGATGAGGAGAAAGAAGAATCATGGGCACTTTTCGGGCTGGACATACAAATAAGGGTACTCAAAGAAGAGTTCAACTTGAATTGGCTGCCAAAGCTTCAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

137

Amino Acids

15.85

Weight (kDa)

4.95

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_13 PF23286 24 - 96 6.8e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0033014)

Species Orthologous Gene IDs
rosa_samantha Rh2BG176700 Rh2DG175400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 288
AcuI CTGAAG 1 cut(s) 314
AfaI GTAC 2 cut(s) 103, 366
AflII CTTAAG 1 cut(s) 284
AgsI TTSAA 4 cut(s) 143, 382, 388, 407
AluBI AGCT 2 cut(s) 67, 403
AluI AGCT 2 cut(s) 67, 403
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 1 cut(s) 232
AlwI GGATC 1 cut(s) 288
AoxI GGCC 1 cut(s) 123
ApeKI GCWGC 1 cut(s) 394
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
AspS9I GGNCC 2 cut(s) 22, 136
AsuHPI GGTGA 2 cut(s) 218, 313
AvaII GGWCC 2 cut(s) 22, 136
BaeGI GKGCMC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 248
BbvCI CCTCAGC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 381
BccI CCATC 1 cut(s) 47
BclI TGATCA 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 232
BfaI CTAG 1 cut(s) 249
BfrI CTTAAG 1 cut(s) 284
BglII AGATCT 1 cut(s) 259
BisI GCNGC 1 cut(s) 395
BlsI GCNGC 1 cut(s) 396
Bme18I GGWCC 2 cut(s) 22, 136
BmgT120I GGNCC 2 cut(s) 22, 136
BmiI GGNNCC 1 cut(s) 24
Bpu10I CCTNAGC 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 106
BseMII CTCAG 1 cut(s) 41
BseRI GAGGAG 1 cut(s) 329
BseSI GKGCMC 1 cut(s) 337
BseXI GCAGC 1 cut(s) 381
BshFI GGCC 1 cut(s) 125
BsiHKAI GWGCWC 1 cut(s) 248
BsiSI CCGG 1 cut(s) 122
BslFI GGGAC 1 cut(s) 8
BsmAI GTCTC 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 8
BsmI GAATGC 1 cut(s) 35
BsnI GGCC 1 cut(s) 125
Bsp1286I GDGCHC 2 cut(s) 248, 337
Bsp143I GATC 4 cut(s) 69, 228, 259, 280
BspANI GGCC 1 cut(s) 125
BspCNI CTCAG 1 cut(s) 40
BspLI GGNNCC 1 cut(s) 24
BspPI GGATC 1 cut(s) 288
BspTI CTTAAG 1 cut(s) 284
BssMI GATC 4 cut(s) 69, 228, 259, 280
Bst4CI ACNGT 1 cut(s) 243
Bst6I CTCTTC 1 cut(s) 369
BstAFI CTTAAG 1 cut(s) 284
BstC8I GCNNGC 1 cut(s) 33
BstDEI CTNAG 1 cut(s) 27
BstKTI GATC 4 cut(s) 72, 231, 262, 283
BstMAI GTCTC 1 cut(s) 232
BstMBI GATC 4 cut(s) 69, 228, 259, 280
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstSLI GKGCMC 1 cut(s) 337
BstV1I GCAGC 1 cut(s) 381
BstX2I RGATCY 2 cut(s) 259, 280
BstYI RGATCY 2 cut(s) 259, 280
BsuRI GGCC 1 cut(s) 125
BtsIMutI CAGTG 1 cut(s) 248
Cac8I GCNNGC 1 cut(s) 33
Cfr13I GGNCC 2 cut(s) 22, 136
Csp6I GTAC 2 cut(s) 102, 365
CviAII CATG 1 cut(s) 330
CviJI RGCY 9 cut(s) 9, 17, 31, 67, 91, 125, 346, 394, 403
CviKI_1 RGCY 9 cut(s) 9, 17, 31, 67, 91, 125, 346, 394, 403
CviQI GTAC 2 cut(s) 102, 365
DdeI CTNAG 1 cut(s) 27
DpnI GATC 4 cut(s) 71, 230, 261, 282
DpnII GATC 4 cut(s) 69, 228, 259, 280
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
Eco47I GGWCC 2 cut(s) 22, 136
Eco57I CTGAAG 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 136
FaeI CATG 1 cut(s) 333
FaiI YATR 6 cut(s) 164, 236, 278, 307, 331, 353
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 1 cut(s) 8
FatI CATG 1 cut(s) 329
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 395
Fsp4HI GCNGC 1 cut(s) 395
FspBI CTAG 1 cut(s) 249
GluI GCNGC 1 cut(s) 395
HaeIII GGCC 1 cut(s) 125
HapII CCGG 1 cut(s) 122
Hin1II CATG 1 cut(s) 333
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 1 cut(s) 326
HpaII CCGG 1 cut(s) 122
HphI GGTGA 2 cut(s) 218, 313
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 4 cut(s) 50, 85, 158, 249
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 1 cut(s) 243
HpyCH4V TGCA 3 cut(s) 4, 35, 256
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
HpyF3I CTNAG 1 cut(s) 27
Hsp92II CATG 1 cut(s) 333
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 4 cut(s) 69, 228, 259, 280
LmnI GCTCC 1 cut(s) 47
LpnPI CCDG 5 cut(s) 45, 63, 135, 207, 332
Lsp1109I GCAGC 1 cut(s) 381
MaeI CTAG 1 cut(s) 249
MalI GATC 4 cut(s) 71, 230, 261, 282
MboI GATC 4 cut(s) 69, 228, 259, 280
MboII GAAGA 2 cut(s) 335, 386
MflI RGATCY 2 cut(s) 259, 280
MhlI GDGCHC 2 cut(s) 248, 337
MluCI AATT 3 cut(s) 74, 129, 388
MnlI CCTC 6 cut(s) 36, 81, 136, 276, 292, 307
MseI TTAA 5 cut(s) 110, 132, 149, 183, 285
MspCI CTTAAG 1 cut(s) 284
MspI CCGG 1 cut(s) 122
Mva1269I GAATGC 1 cut(s) 35
MwoI GCNNNNNNNGC 1 cut(s) 400
NdeII GATC 4 cut(s) 69, 228, 259, 280
NlaIII CATG 1 cut(s) 333
NlaIV GGNNCC 1 cut(s) 24
PctI GAATGC 1 cut(s) 35
PfeI GAWTC 1 cut(s) 326
PkrI GCNGC 1 cut(s) 396
PpuMI RGGWCCY 1 cut(s) 136
Psp5II RGGWCCY 1 cut(s) 136
PspN4I GGNNCC 1 cut(s) 24
PspPI GGNCC 2 cut(s) 22, 136
PspPPI RGGWCCY 1 cut(s) 136
PsuI RGATCY 2 cut(s) 259, 280
RsaI GTAC 2 cut(s) 103, 366
RsaNI GTAC 2 cut(s) 102, 365
SaqAI TTAA 5 cut(s) 110, 132, 149, 183, 285
SatI GCNGC 1 cut(s) 395
Sau3AI GATC 4 cut(s) 69, 228, 259, 280
Sau96I GGNCC 2 cut(s) 22, 136
SduI GDGCHC 2 cut(s) 248, 337
SetI ASST 5 cut(s) 69, 141, 268, 303, 405
SinI GGWCC 2 cut(s) 22, 136
SmlI CTYRAG 2 cut(s) 85, 284
SmoI CTYRAG 2 cut(s) 85, 284
Sse9I AATT 3 cut(s) 74, 129, 388
SspI AATATT 1 cut(s) 410
SspMI CTAG 1 cut(s) 249
TaaI ACNGT 1 cut(s) 243
TaqI TCGA 1 cut(s) 159
TasI AATT 3 cut(s) 74, 129, 388
TatI WGTACW 1 cut(s) 101
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 5 cut(s) 110, 132, 149, 183, 285
Tru9I TTAA 5 cut(s) 110, 132, 149, 183, 285
TscAI CASTG 1 cut(s) 248
TseI GCWGC 1 cut(s) 394
TspRI CASTG 1 cut(s) 248
Vha464I CTTAAG 1 cut(s) 284
VpaK11BI GGWCC 2 cut(s) 22, 136
XbaI TCTAGA 1 cut(s) 248
XspI CTAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.