Rh3AG221700

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0022713
Physical Location & Seq
Reverse (-)
22742043 .. 22742411
369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG221700.1

Sequence Viewer

Length: 369 bp
ATGCCACAATGCCAATACCAACCCCCAAATCCCATCCATGACCCTTGGCCCTTCTGCTCCATCCTGTCGCAATCTGAAACGGCGTCGTTCACCCACGCGCACATCGACTGGAAGGAGACGCCGACGGCGCACGTGTCCAAGGCTGATGTGCCGGGCCTGCGGAAGGACGAGGTCAAGGTGGAGGTGGAGGACGACAAGATTCTTCAGGTCATCGGAGAGCGGAAGCACGATGTGGAAGAGAAGACTGAGACGTGGCACCAGGTCGAGCACAGCAGGCATGAGTCCTCAACGTCACGGTGCCCAAGGAGGAGGCCAAGAAGGTCTATGTCAGGTCAATTGAGATCTCTGGTTAATCAGGCTTATCTGTAA

Protein Analysis

122

Amino Acids

14.19

Weight (kDa)

6.7

Isoelectric Point (pI)

73.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 36 - 93 9.7e-09 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022713)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0479041 RchiOBHm_Chr3g0479071
rosa_samantha Rh3AG221700 Rh3BG254000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 255, 297
AccBSI CCGCTC 1 cut(s) 220
AccII CGCG 1 cut(s) 98
AciI CCGC 2 cut(s) 160, 220
AcuI CTGAAG 1 cut(s) 188
AcvI CACGTG 1 cut(s) 133
AcyI GRCGYC 2 cut(s) 83, 119
AdeI CACNNNGTG 1 cut(s) 232
AfiI CCNNNNNNNGG 1 cut(s) 163
AflIII ACRYGT 1 cut(s) 132
AjiI CACGTC 1 cut(s) 252
AjnI CCWGG 1 cut(s) 258
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 2 cut(s) 110, 242
AoxI GGCC 3 cut(s) 47, 154, 311
AspLEI GCGC 2 cut(s) 100, 130
AspS9I GGNCC 2 cut(s) 48, 154
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 302
BanI GGYRCC 2 cut(s) 255, 297
BbrPI CACGTG 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 248
Bbv12I GWGCWC 1 cut(s) 270
BccI CCATC 2 cut(s) 41, 68
BceAI ACGGC 2 cut(s) 96, 141
BciT130I CCWGG 1 cut(s) 260
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 2 cut(s) 110, 242
BglII AGATCT 1 cut(s) 341
Bme1390I CCNGG 2 cut(s) 153, 260
BmgBI CACGTC 1 cut(s) 252
BmgT120I GGNCC 2 cut(s) 48, 154
BmiI GGNNCC 2 cut(s) 257, 299
BmrFI CCNGG 2 cut(s) 153, 260
BpiI GAAGAC 1 cut(s) 248
BpuMI CCSGG 1 cut(s) 153
BsaAI YACGTR 1 cut(s) 133
BsaHI GRCGYC 2 cut(s) 83, 119
BsaJI CCNNGG 3 cut(s) 44, 138, 302
Bsc4I CCNNNNNNNGG 1 cut(s) 163
Bse1I ACTGG 1 cut(s) 113
BseBI CCWGG 1 cut(s) 260
BseDI CCNNGG 3 cut(s) 44, 138, 302
BseGI GGATG 2 cut(s) 33, 60
BseLI CCNNNNNNNGG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 1 cut(s) 113
BseRI GAGGAG 1 cut(s) 322
BseSI GKGCMC 1 cut(s) 302
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 3 cut(s) 49, 156, 313
BshNI GGYRCC 2 cut(s) 255, 297
BsiHKAI GWGCWC 1 cut(s) 270
BsiSI CCGG 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 163
BsmAI GTCTC 2 cut(s) 110, 242
BsmBI CGTCTC 2 cut(s) 110, 242
BsnI GGCC 3 cut(s) 49, 156, 313
Bsp1286I GDGCHC 2 cut(s) 270, 302
Bsp143I GATC 1 cut(s) 341
BspACI CCGC 2 cut(s) 160, 220
BspANI GGCC 3 cut(s) 49, 156, 313
BspCNI CTCAG 1 cut(s) 238
BspFNI CGCG 1 cut(s) 98
BspLI GGNNCC 2 cut(s) 257, 299
BspT107I GGYRCC 2 cut(s) 255, 297
BsrBI CCGCTC 1 cut(s) 220
BsrI ACTGG 1 cut(s) 113
BssECI CCNNGG 3 cut(s) 44, 138, 302
BssMI GATC 1 cut(s) 341
BssNI GRCGYC 2 cut(s) 83, 119
BssT1I CCWWGG 3 cut(s) 44, 138, 302
Bst2UI CCWGG 1 cut(s) 260
Bst4CI ACNGT 1 cut(s) 297
Bst6I CTCTTC 1 cut(s) 231
BstACI GRCGYC 2 cut(s) 83, 119
BstBAI YACGTR 1 cut(s) 133
BstC8I GCNNGC 2 cut(s) 158, 275
BstDEI CTNAG 1 cut(s) 246
BstENI CCTNNNNNAGG 1 cut(s) 161
BstF5I GGATG 2 cut(s) 33, 60
BstFNI CGCG 1 cut(s) 98
BstHHI GCGC 2 cut(s) 100, 130
BstKTI GATC 1 cut(s) 344
BstMAI GTCTC 2 cut(s) 110, 242
BstMBI GATC 1 cut(s) 341
BstMWI GCNNNNNNNGC 3 cut(s) 127, 157, 274
BstNI CCWGG 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 151, 258
BstSLI GKGCMC 1 cut(s) 302
BstUI CGCG 1 cut(s) 98
BstV2I GAAGAC 1 cut(s) 248
BstX2I RGATCY 1 cut(s) 341
BstYI RGATCY 1 cut(s) 341
BsuRI GGCC 3 cut(s) 49, 156, 313
BtrI CACGTC 1 cut(s) 252
BtsCI GGATG 2 cut(s) 33, 60
Cac8I GCNNGC 2 cut(s) 158, 275
CfoI GCGC 2 cut(s) 100, 130
Cfr13I GGNCC 2 cut(s) 48, 154
CseI GACGC 2 cut(s) 72, 127
CsiI ACCWGGT 1 cut(s) 258
CviAII CATG 2 cut(s) 38, 278
CviJI RGCY 5 cut(s) 49, 143, 156, 313, 359
CviKI_1 RGCY 5 cut(s) 49, 143, 156, 313, 359
DdeI CTNAG 1 cut(s) 246
DpnI GATC 1 cut(s) 343
DpnII GATC 1 cut(s) 341
DraIII CACNNNGTG 1 cut(s) 232
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
Eco130I CCWWGG 3 cut(s) 44, 138, 302
Eco57I CTGAAG 1 cut(s) 188
Eco72I CACGTG 1 cut(s) 133
EcoNI CCTNNNNNAGG 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 258
EcoT14I CCWWGG 3 cut(s) 44, 138, 302
ErhI CCWWGG 3 cut(s) 44, 138, 302
Esp3I CGTCTC 2 cut(s) 110, 242
FaeI CATG 2 cut(s) 41, 281
FaiI YATR 3 cut(s) 39, 279, 326
FatI CATG 2 cut(s) 37, 277
FokI GGATG 2 cut(s) 20, 47
GlaI GCGC 2 cut(s) 99, 129
HaeIII GGCC 3 cut(s) 49, 156, 313
HapII CCGG 1 cut(s) 152
HgaI GACGC 2 cut(s) 72, 127
HhaI GCGC 2 cut(s) 100, 130
Hin1I GRCGYC 2 cut(s) 83, 119
Hin1II CATG 2 cut(s) 41, 281
Hin6I GCGC 2 cut(s) 98, 128
HinP1I GCGC 2 cut(s) 98, 128
HinfI GANTC 2 cut(s) 199, 281
HpaII CCGG 1 cut(s) 152
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 1 cut(s) 90
Hpy188I TCNGA 2 cut(s) 76, 215
Hpy8I GTNNAC 1 cut(s) 90
Hpy99I CGWCG 2 cut(s) 88, 127
HpyAV CCTTC 4 cut(s) 61, 106, 157, 312
HpyCH4III ACNGT 1 cut(s) 297
HpyCH4IV ACGT 3 cut(s) 132, 251, 290
HpyF10VI GCNNNNNNNGC 3 cut(s) 127, 157, 274
HpyF3I CTNAG 1 cut(s) 246
HpySE526I ACGT 3 cut(s) 132, 251, 290
Hsp92I GRCGYC 2 cut(s) 83, 119
Hsp92II CATG 2 cut(s) 41, 281
HspAI GCGC 2 cut(s) 98, 128
Kzo9I GATC 1 cut(s) 341
LmnI GCTCC 1 cut(s) 62
MabI ACCWGGT 1 cut(s) 258
MaeII ACGT 3 cut(s) 132, 251, 290
MaeIII GTNAC 1 cut(s) 291
MalI GATC 1 cut(s) 343
MbiI CCGCTC 1 cut(s) 220
MboI GATC 1 cut(s) 341
MboII GAAGA 3 cut(s) 194, 248, 253
MfeI CAATTG 1 cut(s) 335
MflI RGATCY 1 cut(s) 341
MhlI GDGCHC 2 cut(s) 270, 302
MluCI AATT 1 cut(s) 335
MlyI GAGTC 1 cut(s) 290
MnlI CCTC 6 cut(s) 163, 175, 181, 295, 300, 303
MseI TTAA 1 cut(s) 351
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 2 cut(s) 153, 260
MunI CAATTG 1 cut(s) 335
MvaI CCWGG 1 cut(s) 260
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 3 cut(s) 127, 157, 274
NciI CCSGG 1 cut(s) 153
NdeII GATC 1 cut(s) 341
NlaIII CATG 2 cut(s) 41, 281
NlaIV GGNNCC 2 cut(s) 257, 299
NmuCI GTSAC 1 cut(s) 291
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PfeI GAWTC 1 cut(s) 199
PflFI GACNNNGTC 1 cut(s) 170
PleI GAGTC 1 cut(s) 289
PmaCI CACGTG 1 cut(s) 133
PmlI CACGTG 1 cut(s) 133
PpsI GAGTC 1 cut(s) 289
Ppu21I YACGTR 1 cut(s) 133
Psp6I CCWGG 1 cut(s) 258
PspCI CACGTG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 258
PspN4I GGNNCC 2 cut(s) 257, 299
PspPI GGNCC 2 cut(s) 48, 154
PsuI RGATCY 1 cut(s) 341
PsyI GACNNNGTC 1 cut(s) 170
SaqAI TTAA 1 cut(s) 351
Sau3AI GATC 1 cut(s) 341
Sau96I GGNCC 2 cut(s) 48, 154
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 2 cut(s) 153, 260
SduI GDGCHC 2 cut(s) 270, 302
SexAI ACCWGGT 1 cut(s) 258
Sse9I AATT 1 cut(s) 335
SsiI CCGC 2 cut(s) 160, 220
StyD4I CCNGG 2 cut(s) 151, 258
StyI CCWWGG 3 cut(s) 44, 138, 302
TaaI ACNGT 1 cut(s) 297
TaiI ACGT 3 cut(s) 135, 254, 293
TaqI TCGA 2 cut(s) 105, 264
TasI AATT 1 cut(s) 335
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TseFI GTSAC 1 cut(s) 291
Tsp45I GTSAC 1 cut(s) 291
Tth111I GACNNNGTC 1 cut(s) 170
XagI CCTNNNNNAGG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.