Rh3DG287000

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0010489
Physical Location & Seq
Reverse (-)
29616095 .. 29618900
2806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG287000.1

Sequence Viewer

Length: 855 bp
ATGGCTGAGCATGACCTAACCCCGCGCATCGCGCCCAACCTGGACAGGCACCTGGTGTTCCCTCTGATGGAGTTTCTTCAGGAGAGGCAGCTCTACCCGGACGAGCAGATCCTCAAGTCCAAGATCGAGCTCCTCAACAAGACCAACATGGTCGACTACGCCATGGACATCCACAAGTCCCTCTATCACACCGAAGATGTCCCCCCGGAGATGGTCGAGATGAGGGCCGAGGTGGTCGCTCGCCTCAAGGCTCTCGAGGAGTCCGCGGCGCCCCTTGTCAACTTCCTGCAGAACGCCGCCGCCGTTCAGGAGCTCCGGTCTGACAAGCAGTACAATCTCCAGATGCTCAATGACCGTTACCAGAATTTCAGTTCACCTATGAATCAGGTGCAAAACAGAATATGGTTGATGCACTGGAGTCTCTTCATATTTTTTAACCACGACAATGGAAGAACTCAAATCATTGAACTGTTTAATCAGGACAAGTATCTGAATGCTATCCAAACCAATGCTCCCCATCTCATTCGTTACTTGGCCACTGCATTCATTGTCAATAAGAGAAGGAGACCTCAATTCAAGGATTTTATAAAGCCCCACCCTCCTCCCAAGGCTCGTTACACGGAAGTGCTTGCTGAAAAATTGAATTTGAATTATGAGGAGGCTGAGAGATGGATTGTGAATCTTATTCGGAGCTCAAAGCTTGACGCAAAGATCGACTCACAGTCAGGAACTGTTATCATGGAACCTAACCATCCAAATGTGTATGAGCAACTGATAGATCACACTAAGGCATTATCTGGGCGTACTTACAAGTTAGTTAATCAGATTAGGGAACATGCAAGTGGAAAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000502 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005852 GO:0005886 GO:0006139 GO:0006351 GO:0006352 GO:0006412 GO:0006413 GO:0006417 GO:0006518 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009651 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016020 GO:0016070 GO:0017148 GO:0018130 GO:0019222 GO:0019438 GO:0019538 GO:0022414 GO:0030371 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031597 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034248 GO:0034249 GO:0034641 GO:0034645 GO:0034654 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0045182 GO:0046483 GO:0048367 GO:0048519 GO:0048523 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0080090 GO:0090304 GO:0090567 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1905368 GO:1905369 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
ko03013 ko05160 map03013 map05160
Pfam Domains
Protein Families
PCI eIF3_N

Protein Analysis

284

Amino Acids

33.36

Weight (kDa)

7.21

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3_N PF09440 5 - 121 2.4e-41 eIF3 subunit 6 N terminal domain
PCI PF01399 208 - 248 8.4e-10 PCI domain
EIF3E_C PF21357 253 - 273 3.3e-07 Eukaryotic translation initiation factor 3 subunit E, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 587
AccB1I GGYRCC 2 cut(s) 48, 268
AccI GTMKAC 1 cut(s) 153
AccII CGCG 3 cut(s) 25, 32, 266
AciI CCGC 5 cut(s) 23, 264, 266, 297, 300
AclWI GGATC 1 cut(s) 103
AcoI YGGCCR 1 cut(s) 534
AcsI RAATTY 2 cut(s) 364, 643
AcuI CTGAAG 1 cut(s) 62
AcyI GRCGYC 1 cut(s) 269
AdeI CACNNNGTG 1 cut(s) 55
AfaI GTAC 2 cut(s) 332, 805
AfiI CCNNNNNNNGG 2 cut(s) 67, 211
AgsI TTSAA 4 cut(s) 467, 577, 643, 649
AhdI GACNNNNNGTC 1 cut(s) 721
AjnI CCWGG 2 cut(s) 39, 51
AleI CACNNNNGTG 1 cut(s) 623
AluBI AGCT 5 cut(s) 91, 130, 313, 693, 700
AluI AGCT 5 cut(s) 91, 130, 313, 693, 700
Alw21I GWGCWC 3 cut(s) 132, 315, 695
Alw26I GTCTC 2 cut(s) 425, 559
AlwI GGATC 1 cut(s) 103
AlwNI CAGNNNCTG 1 cut(s) 731
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 2 cut(s) 225, 534
ApeKI GCWGC 1 cut(s) 88
ApoI RAATTY 2 cut(s) 364, 643
AspLEI GCGC 3 cut(s) 27, 34, 271
AspS9I GGNCC 1 cut(s) 225
AsuC2I CCSGG 2 cut(s) 98, 206
AsuHPI GGTGA 1 cut(s) 366
AvaI CYCGRG 1 cut(s) 254
BalI TGGCCA 1 cut(s) 536
BanI GGYRCC 2 cut(s) 48, 268
BanII GRGCYC 3 cut(s) 132, 315, 695
Bbv12I GWGCWC 3 cut(s) 132, 315, 695
BbvI GCAGC 1 cut(s) 100
BccI CCATC 5 cut(s) 61, 205, 525, 663, 759
BceAI ACGGC 1 cut(s) 287
BciT130I CCWGG 2 cut(s) 41, 53
BcnI CCSGG 2 cut(s) 98, 206
BcoDI GTCTC 2 cut(s) 425, 559
BfaI CTAG 1 cut(s) 853
BfmI CTRYAG 1 cut(s) 287
BfoI RGCGCY 1 cut(s) 272
BisI GCNGC 4 cut(s) 89, 267, 297, 300
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 4 cut(s) 90, 268, 298, 301
Bme1390I CCNGG 4 cut(s) 41, 53, 98, 206
BmeRI GACNNNNNGTC 1 cut(s) 721
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 225
BmiI GGNNCC 3 cut(s) 50, 270, 744
BmrFI CCNGG 4 cut(s) 41, 53, 98, 206
BmsI GCATC 3 cut(s) 36, 333, 399
BpmI CTGGAG 2 cut(s) 323, 436
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 2 cut(s) 98, 230
BpuMI CCSGG 2 cut(s) 98, 206
BsaHI GRCGYC 1 cut(s) 269
BsaI GGTCTC 1 cut(s) 559
BsaJI CCNNGG 5 cut(s) 162, 204, 228, 264, 606
BsaWI WCCGGW 1 cut(s) 315
BsaXI ACNNNNNCTCC 4 cut(s) 302, 332, 496, 526
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 211
Bse1I ACTGG 1 cut(s) 419
BseBI CCWGG 2 cut(s) 41, 53
BseDI CCNNGG 5 cut(s) 162, 204, 228, 264, 606
BseGI GGATG 2 cut(s) 168, 751
BseLI CCNNNNNNNGG 2 cut(s) 67, 211
BseMII CTCAG 1 cut(s) 654
BseNI ACTGG 1 cut(s) 419
BseRI GAGGAG 4 cut(s) 122, 272, 591, 671
BseXI GCAGC 1 cut(s) 100
Bsh1236I CGCG 3 cut(s) 25, 32, 266
BshFI GGCC 2 cut(s) 227, 536
BshNI GGYRCC 2 cut(s) 48, 268
BsiHKAI GWGCWC 3 cut(s) 132, 315, 695
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 3 cut(s) 98, 206, 316
BslFI GGGAC 2 cut(s) 163, 185
BslI CCNNNNNNNGG 2 cut(s) 67, 211
BsmAI GTCTC 2 cut(s) 425, 559
BsmFI GGGAC 2 cut(s) 163, 185
BsmI GAATGC 2 cut(s) 499, 542
BsnI GGCC 2 cut(s) 227, 536
Bso31I GGTCTC 1 cut(s) 559
BsoBI CYCGRG 1 cut(s) 254
Bsp1286I GDGCHC 3 cut(s) 132, 315, 695
Bsp143I GATC 4 cut(s) 108, 123, 711, 778
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 5 cut(s) 23, 264, 266, 297, 300
BspANI GGCC 2 cut(s) 227, 536
BspCNI CTCAG 1 cut(s) 655
BspFNI CGCG 3 cut(s) 25, 32, 266
BspLI GGNNCC 3 cut(s) 50, 270, 744
BspMAI CTGCAG 1 cut(s) 291
BspPI GGATC 1 cut(s) 103
BspT107I GGYRCC 2 cut(s) 48, 268
BspTNI GGTCTC 1 cut(s) 559
BsrI ACTGG 1 cut(s) 419
BssECI CCNNGG 5 cut(s) 162, 204, 228, 264, 606
BssMI GATC 4 cut(s) 108, 123, 711, 778
BssNI GRCGYC 1 cut(s) 269
BssT1I CCWWGG 2 cut(s) 162, 606
Bst2UI CCWGG 2 cut(s) 41, 53
Bst4CI ACNGT 4 cut(s) 356, 471, 723, 733
Bst6I CTCTTC 1 cut(s) 428
BstACI GRCGYC 1 cut(s) 269
BstC8I GCNNGC 2 cut(s) 241, 630
BstDEI CTNAG 3 cut(s) 6, 663, 786
BstDSI CCRYGG 2 cut(s) 162, 264
BstF5I GGATG 2 cut(s) 168, 751
BstFNI CGCG 3 cut(s) 25, 32, 266
BstH2I RGCGCY 1 cut(s) 272
BstHHI GCGC 3 cut(s) 27, 34, 271
BstKTI GATC 4 cut(s) 111, 126, 714, 781
BstMAI GTCTC 2 cut(s) 425, 559
BstMBI GATC 4 cut(s) 108, 123, 711, 778
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstNI CCWGG 2 cut(s) 41, 53
BstNSI RCATGY 1 cut(s) 839
BstSCI CCNGG 4 cut(s) 39, 51, 96, 204
BstSFI CTRYAG 1 cut(s) 287
BstUI CGCG 3 cut(s) 25, 32, 266
BstV1I GCAGC 1 cut(s) 100
BstX2I RGATCY 1 cut(s) 108
BstXI CCANNNNNNTGG 1 cut(s) 446
BstYI RGATCY 1 cut(s) 108
BsuRI GGCC 2 cut(s) 227, 536
BtgI CCRYGG 2 cut(s) 162, 264
BtgZI GCGATG 1 cut(s) 13
BtsCI GGATG 2 cut(s) 168, 751
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 2 cut(s) 412, 537
Cac8I GCNNGC 2 cut(s) 241, 630
CaiI CAGNNNCTG 1 cut(s) 731
CfoI GCGC 3 cut(s) 27, 34, 271
Cfr13I GGNCC 1 cut(s) 225
Cfr42I CCGCGG 1 cut(s) 267
CseI GACGC 1 cut(s) 713
CsiI ACCWGGT 1 cut(s) 51
Csp6I GTAC 2 cut(s) 331, 804
CviAII CATG 5 cut(s) 11, 148, 163, 739, 836
CviQI GTAC 2 cut(s) 331, 804
DdeI CTNAG 3 cut(s) 6, 663, 786
DinI GGCGCC 1 cut(s) 270
DpnI GATC 4 cut(s) 110, 125, 713, 780
DpnII GATC 4 cut(s) 108, 123, 711, 778
DraIII CACNNNGTG 1 cut(s) 55
DriI GACNNNNNGTC 1 cut(s) 721
EaeI YGGCCR 1 cut(s) 534
Eam1104I CTCTTC 1 cut(s) 428
Eam1105I GACNNNNNGTC 1 cut(s) 721
EarI CTCTTC 1 cut(s) 428
Ecl136II GAGCTC 3 cut(s) 130, 313, 693
Eco130I CCWWGG 2 cut(s) 162, 606
Eco24I GRGCYC 3 cut(s) 132, 315, 695
Eco31I GGTCTC 1 cut(s) 559
Eco53kI GAGCTC 3 cut(s) 130, 313, 693
Eco57I CTGAAG 1 cut(s) 62
Eco88I CYCGRG 1 cut(s) 254
EcoICRI GAGCTC 3 cut(s) 130, 313, 693
EcoRII CCWGG 2 cut(s) 39, 51
EcoT14I CCWWGG 2 cut(s) 162, 606
EcoT38I GRGCYC 3 cut(s) 132, 315, 695
EgeI GGCGCC 1 cut(s) 270
EheI GGCGCC 1 cut(s) 270
ErhI CCWWGG 2 cut(s) 162, 606
FaeI CATG 5 cut(s) 14, 151, 166, 742, 839
FaqI GGGAC 2 cut(s) 163, 185
FatI CATG 5 cut(s) 10, 147, 162, 738, 835
FauI CCCGC 1 cut(s) 30
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 4 cut(s) 89, 267, 297, 300
FokI GGATG 2 cut(s) 155, 738
FriOI GRGCYC 3 cut(s) 132, 315, 695
Fsp4HI GCNGC 4 cut(s) 89, 267, 297, 300
FspBI CTAG 1 cut(s) 853
GlaI GCGC 3 cut(s) 26, 33, 270
GluI GCNGC 4 cut(s) 89, 267, 297, 300
GsuI CTGGAG 2 cut(s) 323, 436
HaeII RGCGCY 1 cut(s) 272
HaeIII GGCC 2 cut(s) 227, 536
HapII CCGG 3 cut(s) 98, 206, 316
HgaI GACGC 1 cut(s) 713
HhaI GCGC 3 cut(s) 27, 34, 271
Hin1I GRCGYC 1 cut(s) 269
Hin1II CATG 5 cut(s) 14, 151, 166, 742, 839
Hin6I GCGC 3 cut(s) 25, 32, 269
HinP1I GCGC 3 cut(s) 25, 32, 269
HincII GTYRAC 2 cut(s) 154, 280
HindII GTYRAC 2 cut(s) 154, 280
HindIII AAGCTT 1 cut(s) 698
HinfI GANTC 5 cut(s) 260, 382, 418, 679, 716
HpaII CCGG 3 cut(s) 98, 206, 316
HphI GGTGA 1 cut(s) 366
Hpy166II GTNNAC 3 cut(s) 154, 280, 374
Hpy188I TCNGA 5 cut(s) 66, 322, 492, 690, 825
Hpy188III TCNNGA 7 cut(s) 80, 217, 254, 308, 340, 479, 726
Hpy8I GTNNAC 3 cut(s) 154, 280, 374
HpyAV CCTTC 1 cut(s) 555
HpyCH4III ACNGT 4 cut(s) 356, 471, 723, 733
HpyCH4V TGCA 5 cut(s) 289, 391, 412, 542, 839
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 3 cut(s) 6, 663, 786
Hsp92I GRCGYC 1 cut(s) 269
Hsp92II CATG 5 cut(s) 14, 151, 166, 742, 839
HspAI GCGC 3 cut(s) 25, 32, 269
KasI GGCGCC 1 cut(s) 268
KspI CCGCGG 1 cut(s) 267
Kzo9I GATC 4 cut(s) 108, 123, 711, 778
LmnI GCTCC 5 cut(s) 135, 310, 318, 517, 690
Lsp1109I GCAGC 1 cut(s) 100
LweI GCATC 3 cut(s) 36, 333, 399
MabI ACCWGGT 1 cut(s) 51
MaeI CTAG 1 cut(s) 853
MaeIII GTNAC 3 cut(s) 356, 527, 614
MalI GATC 4 cut(s) 110, 125, 713, 780
MboI GATC 4 cut(s) 108, 123, 711, 778
MboII GAAGA 4 cut(s) 68, 206, 415, 462
MflI RGATCY 1 cut(s) 108
MhlI GDGCHC 3 cut(s) 132, 315, 695
MlsI TGGCCA 1 cut(s) 536
MluCI AATT 5 cut(s) 364, 572, 638, 643, 649
MluNI TGGCCA 1 cut(s) 536
Mly113I GGCGCC 1 cut(s) 269
MlyI GAGTC 3 cut(s) 269, 427, 710
Mox20I TGGCCA 1 cut(s) 536
MscI TGGCCA 1 cut(s) 536
MseI TTAA 3 cut(s) 435, 474, 819
MslI CAYNNNNRTG 4 cut(s) 444, 623, 756, 840
Msp20I TGGCCA 1 cut(s) 536
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 3 cut(s) 98, 206, 316
MspR9I CCNGG 4 cut(s) 41, 53, 98, 206
Mva1269I GAATGC 2 cut(s) 499, 542
MvaI CCWGG 2 cut(s) 41, 53
MvnI CGCG 3 cut(s) 25, 32, 266
MwoI GCNNNNNNNGC 1 cut(s) 31
NarI GGCGCC 1 cut(s) 269
NciI CCSGG 2 cut(s) 98, 206
NcoI CCATGG 1 cut(s) 162
NdeII GATC 4 cut(s) 108, 123, 711, 778
NlaIII CATG 5 cut(s) 14, 151, 166, 742, 839
NlaIV GGNNCC 3 cut(s) 50, 270, 744
NmeAIII GCCGAG 1 cut(s) 253
NspI RCATGY 1 cut(s) 839
OliI CACNNNNGTG 1 cut(s) 623
PaeR7I CTCGAG 1 cut(s) 254
PcsI WCGNNNNNNNCGW 2 cut(s) 300, 711
PctI GAATGC 2 cut(s) 499, 542
PfeI GAWTC 2 cut(s) 382, 679
PkrI GCNGC 4 cut(s) 90, 268, 298, 301
PleI GAGTC 3 cut(s) 268, 426, 710
PluTI GGCGCC 1 cut(s) 272
PpsI GAGTC 3 cut(s) 268, 426, 710
PsiI TTATAA 1 cut(s) 587
Psp124BI GAGCTC 3 cut(s) 132, 315, 695
Psp6I CCWGG 2 cut(s) 39, 51
PspGI CCWGG 2 cut(s) 39, 51
PspN4I GGNNCC 3 cut(s) 50, 270, 744
PspPI GGNCC 1 cut(s) 225
PstI CTGCAG 1 cut(s) 291
PstNI CAGNNNCTG 1 cut(s) 731
PsuI RGATCY 1 cut(s) 108
RsaI GTAC 2 cut(s) 332, 805
RsaNI GTAC 2 cut(s) 331, 804
RseI CAYNNNNRTG 4 cut(s) 444, 623, 756, 840
SacI GAGCTC 3 cut(s) 132, 315, 695
SacII CCGCGG 1 cut(s) 267
SalI GTCGAC 1 cut(s) 152
SaqAI TTAA 3 cut(s) 435, 474, 819
SatI GCNGC 4 cut(s) 89, 267, 297, 300
Sau3AI GATC 4 cut(s) 108, 123, 711, 778
Sau96I GGNCC 1 cut(s) 225
SchI GAGTC 3 cut(s) 269, 427, 710
ScrFI CCNGG 4 cut(s) 41, 53, 98, 206
SduI GDGCHC 3 cut(s) 132, 315, 695
SexAI ACCWGGT 1 cut(s) 51
SfaNI GCATC 3 cut(s) 36, 333, 399
SfcI CTRYAG 1 cut(s) 287
SfoI GGCGCC 1 cut(s) 270
Sfr274I CTCGAG 1 cut(s) 254
Sfr303I CCGCGG 1 cut(s) 267
SgrBI CCGCGG 1 cut(s) 267
SlaI CTCGAG 1 cut(s) 254
SmiMI CAYNNNNRTG 4 cut(s) 444, 623, 756, 840
SmlI CTYRAG 3 cut(s) 113, 245, 254
SmoI CTYRAG 3 cut(s) 113, 245, 254
Sse9I AATT 5 cut(s) 364, 572, 638, 643, 649
SsiI CCGC 5 cut(s) 23, 264, 266, 297, 300
SspDI GGCGCC 1 cut(s) 268
SspMI CTAG 1 cut(s) 853
SstI GAGCTC 3 cut(s) 132, 315, 695
StyD4I CCNGG 4 cut(s) 39, 51, 96, 204
StyI CCWWGG 2 cut(s) 162, 606
TaaI ACNGT 4 cut(s) 356, 471, 723, 733
TaqI TCGA 5 cut(s) 126, 153, 216, 255, 714
TasI AATT 5 cut(s) 364, 572, 638, 643, 649
TatI WGTACW 1 cut(s) 330
TauI GCSGC 3 cut(s) 269, 299, 302
TfiI GAWTC 2 cut(s) 382, 679
Tru1I TTAA 3 cut(s) 435, 474, 819
Tru9I TTAA 3 cut(s) 435, 474, 819
TscAI CASTG 2 cut(s) 419, 544
TseI GCWGC 1 cut(s) 88
TspDTI ATGAA 3 cut(s) 395, 415, 535
TspGWI ACGGA 1 cut(s) 635
TspRI CASTG 2 cut(s) 419, 544
XapI RAATTY 2 cut(s) 364, 643
XceI RCATGY 1 cut(s) 839
XhoI CTCGAG 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 153
XspI CTAG 1 cut(s) 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.