Rh4AG071500
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0026045
Physical Location & Seq
Forward (+)
14696772 .. 14699855
3084 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG071500.1

Sequence Viewer

Length: 3084 bp
ATGTCCAGAAAATTTGTATCAAGGAGGGCTTCGGAATTGGAGTTTGCGGGAATCTTACTTGCTCTATTTCTACTTGTTTGCACGTCAGAGGGGTTGAATTCTGAAGGGCTTTTCCTTTTAGAGCTGAAGAAGAGTATTTTGGATGAAGCAAATAATTTGGGAAGTTGGAACTCTGCTGATCAAACACCCTGCGGATGGATGGGTGTGAATTGCACTTCTGGTTATGAGCCACTGGTCCAAGGTCTTGATTTGAAATCCATGAATCTTTCAGGAACCCTGAGCCCTAGCATTGGTGGTTTACTTCACCTAACCTCACTTGATCTTGCATCCAATGGTTTCTCTGGAGGCATACCCAATGAGATTGGAAACTGCTCAAGTTTGGAATACCTTTATCTCAATGACAACAAGTTTACTGGCCAAATACCTGCTGAATTGGGTAAGCTGTCCAAATTAAGAAGTTTGAATCTCTGCAACAACAAAATCTCTGGTCCTCTTCCTGAGGAACTTGGGAACTTGTCTTCACTGGTTGAGTTTGTGGCATACACCAACAATATTACCGGTTCAATACCTCATTCTTTTGGGAATCTCAAAAGTTTGGTAACTTTTCGAGCTGGGCAGAATGCTATTTCTGGTAGCATACCAGCTGAAATAGGTGGATGTCAGAACTTGAAACTGCTTGGTCTTGCTCAAAATGTTATAGGAGGGGAGTTACCTAAGGAGCTTGGGATGCTGGGGAGCATGACAGATCTGATTCTGTGGGATAATCAGGTTTCGGGGTTTATTCCTAAGGAGCTTGGAAATTGCAGTAGTCTTGGGACGATTGCTTTGTATGAGAACAATCTCGTGGGGTCAATACCTCCAGAAATTGGGAATCTCAAGTCATTGAGGAGGTTGTACCTCTACAGGAATGGCTTGAATGGAACCATTCCCAGGGAAATTGGAAATCTTTCTTTTGCTGCTGAGATTGATTTTTCGGAGAACTATTTGATGGGTGAGATCCCATATGAGTTAAGTAAGATAAGTGGTCTAAGCTTACTGTACCTTTTCCAGAACCAGCTCACAGGTGTGATTCCAAATGAGCTTAGTAACTTGAGGAAATTATCAAAGCTTGACCTTTCAATTAATCACCTTGAAGGTCTTATTCCATATGGGTTTCAATATTTGACTGAATTGAGCCAGTTACAGTTGTTCGATAACTCTCTAAGGGGTAGCATACCCCTGTGGCTTGGGCGACACAGTCGACTTTGGGTAGTTGATCTTTCAGATAACTCCCTAACTGGGAGAATACCTCCGTACCTTTGTTGGCATTCCAATCTAATCTTGTTGAACCTGGAGTCTAATGATCTTTATGGAAGTATTCCAACTGGAGTTTTGAACTGTGAATCATTGGTGCAACTTCGCCTTGTTGGAAACAGGCTTACTGGCAGCTTTCCTTCTGAGTTATGCAATTTGGTGAACCTTTCTGCGATTGAGTTGGACGGGAACAAGTTCACTGGGTCTATTCCTCCCGAGATTAAAAACTGCCAAAAGTTGCAAAGGCTTCACATTGCAGACAATTACTTCACATCCGAGTTGCCTAGGGAAATAGGATATCTATCTCAATTGGTAACTTTCAACATATCATCCAATTCACTCACTGGGCAAATTCCGCCTGAAATTGTTAACTGCAAGATGCTTCAGCGACTTGACCTCAGTCGGAATAGATTCGAAAATGCTTTGCCAAATGAGCTGGGAACGCTTTTGCAGTTGGAGCTTCTGAGGCTTTCAGAAAATAGTTTTACTGGAAACATACCAGCAGCACTAGGAAACCTCTCACATTTGACAGAGTTGCAGATGGGTGGCAACTTGTTTTCTGGTGAAATACCACCTGAATTGGGTTCTCTTTCAAGCTTGCAGATTGCAATGAATCTCAGTTTTAATAATCTCAGCGGGAGAATACCACCAGAGCTTGGAAATCTAATTTTACTTGAATTCCTTCTGCTCAATAACAATAATTTGACCGGTGAAATTCCCAGCACATTTGAAAATTTGTCAAGCTTAATGGGCTGCAACTTCTCTTACAATGACCTTACTGGATCTTTACCTCCCATCCCATTGTTCCAGAACATGGCAATTAGCAGCTTTATCGGGAACAAAGGTCTTTGTGGTGGGCCTCTTGGTGTGTGCAGTGTAAATTCATCTCCAAATTCTGTTCCATCTCTGAATAGAGTGGATACTCCTCGCAGTAAAATCATTACAATAATTGCAGCTGTTGTGGGTGGTATTTCTCTTATTTTGATTGCTGTTATCTTATATTTCATGAGACGTCCAGGTCAGACGGTTCCTTCCATGCAAGATAAGGATACTTTACCTCCTGATACAGATATATACTTACCTCCAAAGGAGGGGATCACGTTCCAAGACCTGGTTGAGGCGACAAATAACTTTCATGACAGCTATGCTGTTGGAAGGGGAGCTTGTGGAACAGTTTACAAGGCAGTAATGCAGTCTGGACTTACTATTGCTGTTAAGAAGCTTTCAGCTAACCGGGAGGGCAACAACATTGAAAACAGTTTCCAGGCTGAGATTTTAACTCTGGGAAATATAAGGCATCGGAATATTGTGAAGCTCTATGGATTTTGTTATCACAAGGGTTCCAACCTACTTCTTTATGAGTACATGGAAAAGGGTAGCTTGGGTGAATTACTTCATGGTGAATCTTGCAGCTTGGAGTGGCCAACTCGTTTCATGATTGCTCTTGGAGCTGCTGAAGGTCTTGCTTATTTACATCATGACTGCAAACCGAGGATTGTCCACCGTGATATAAAGTCCAATAATATTTTGCTAGATGAGAAGTTCGAAGCCCATGTTGGTGACTTTGGTTTGGCAAAAGTGATTGATATGCCTCATTCAAAATCAATGTCAGCAGTTGCAGGATCATATGGATATATTGCTCCTGGTAAGCTTTACTTTTGTTCACTTTACTTGAATATTATTGGATTACTACATTCATTGAGAATCCTCAAAAACAAAGGTTTGATTTATAGTCTCTCTAAGATTCATAAATTCATCCCCCACCCCAAAAATGAATTTTATTGTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1027

Amino Acids

112.29

Weight (kDa)

5.88

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 32 - 72 1.2e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 82 - 199 2.9e-09 Leucine-rich repeat region
LRR_14 PF23598 288 - 394 2.5e-06 Leucine-rich repeat region
LRR_8 PF13855 510 - 569 3.4e-06 Leucine rich repeat
LRR_14 PF23598 546 - 633 5.1e-08 Leucine-rich repeat region
Pkinase PF00069 810 - 979 3.8e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 814 - 967 5.1e-31 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0026045)

Species Orthologous Gene IDs
rosa_samantha Rh4AG071500 Rh4CG077400 Rh4DG066800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2310
AatII GACGTC 1 cut(s) 2308
Acc36I ACCTGC 1 cut(s) 433
AccB7I CCANNNNNTGG 4 cut(s) 866, 1949, 2107, 2404
AccI GTMKAC 1 cut(s) 1240
AciI CCGC 4 cut(s) 47, 192, 1649, 1929
AclWI GGATC 4 cut(s) 991, 2083, 2396, 2923
AcoI YGGCCR 2 cut(s) 415, 2714
AcuI CTGAAG 4 cut(s) 123, 146, 1661, 2769
AcyI GRCGYC 1 cut(s) 2305
AfaI GTAC 4 cut(s) 896, 1040, 1295, 2657
AgeI ACCGGT 2 cut(s) 557, 2000
AjiI CACGTC 1 cut(s) 84
AjnI CCWGG 6 cut(s) 929, 1329, 2308, 2403, 2556, 2935
AjuI GAANNNNNNNTTGG 4 cut(s) 122, 154, 2832, 2864
AleI CACNNNNGTG 1 cut(s) 1064
Alw26I GTCTC 2 cut(s) 2296, 3032
AlwI GGATC 4 cut(s) 991, 2083, 2396, 2923
Ama87I CYCGRG 1 cut(s) 1508
AoxI GGCC 3 cut(s) 415, 2150, 2714
ApeKI GCWGC 8 cut(s) 956, 1425, 1796, 2046, 2118, 2246, 2703, 2744
ArsI GACNNNNNNTTYG 4 cut(s) 808, 840, 2885, 2917
AseI ATTAAT 1 cut(s) 1122
AsiGI ACCGGT 2 cut(s) 557, 2000
Asp700I GAANNNNTTC 5 cut(s) 946, 1487, 1703, 1974, 2685
AspA2I CCTAGG 1 cut(s) 1577
AspS9I GGNCC 3 cut(s) 235, 488, 2150
AsuC2I CCSGG 1 cut(s) 2528
AsuHPI GGTGA 9 cut(s) 296, 1004, 1118, 1465, 1868, 2015, 2690, 2705, 2864
AsuII TTCGAA 2 cut(s) 1707, 2838
AvaI CYCGRG 1 cut(s) 1508
AvaII GGWCC 2 cut(s) 235, 488
AvrII CCTAGG 1 cut(s) 1577
AxyI CCTNAGG 3 cut(s) 498, 714, 786
BaeI ACNNNNGTAYC 2 cut(s) 1277, 1310
BalI TGGCCA 2 cut(s) 417, 2716
BanII GRGCYC 1 cut(s) 284
BauI CACGAG 1 cut(s) 842
BbsI GAAGAC 1 cut(s) 510
BbvI GCAGC 8 cut(s) 943, 1437, 1808, 2033, 2130, 2258, 2715, 2731
BccI CCATC 6 cut(s) 189, 193, 982, 1828, 2096, 2203
BcgI CGANNNNNNTGC 2 cut(s) 2107, 2141
BciT130I CCWGG 6 cut(s) 931, 1331, 2310, 2405, 2558, 2937
BciVI GTATCC 2 cut(s) 2206, 2335
BclI TGATCA 1 cut(s) 178
BcnI CCSGG 1 cut(s) 2528
BcoDI GTCTC 2 cut(s) 2296, 3032
BfaI CTAG 4 cut(s) 285, 1578, 1802, 2825
BfmI CTRYAG 1 cut(s) 901
BfuAI ACCTGC 1 cut(s) 433
BfuI GTATCC 2 cut(s) 2206, 2335
BglII AGATCT 1 cut(s) 745
BisI GCNGC 8 cut(s) 957, 1426, 1797, 2047, 2119, 2247, 2704, 2745
BlnI CCTAGG 1 cut(s) 1577
BlsI GCNGC 8 cut(s) 958, 1427, 1798, 2048, 2120, 2248, 2705, 2746
Bme1390I CCNGG 7 cut(s) 931, 1331, 2310, 2405, 2528, 2558, 2937
Bme18I GGWCC 2 cut(s) 235, 488
BmeT110I CYCGRG 1 cut(s) 1508
BmgBI CACGTC 1 cut(s) 84
BmgT120I GGNCC 3 cut(s) 235, 488, 2150
BmiI GGNNCC 4 cut(s) 274, 922, 2322, 2635
BmrFI CCNGG 7 cut(s) 931, 1331, 2310, 2405, 2528, 2558, 2937
BmrI ACTGGG 3 cut(s) 1287, 1503, 1647
BmsI GCATC 4 cut(s) 335, 717, 1662, 2599
BmuI ACTGGG 3 cut(s) 1287, 1503, 1647
BoxI GACNNNNGTC 1 cut(s) 1692
BpiI GAAGAC 1 cut(s) 510
BplI GAGNNNNNCTC 2 cut(s) 1273, 1305
BpmI CTGGAG 4 cut(s) 363, 843, 1352, 1386
Bpu10I CCTNAGC 1 cut(s) 278
Bpu14I TTCGAA 2 cut(s) 1707, 2838
BpuEI CTTGAG 3 cut(s) 358, 860, 1111
BpuMI CCSGG 1 cut(s) 2528
BsaBI GATNNNNATC 1 cut(s) 1594
BsaHI GRCGYC 1 cut(s) 2305
BsaJI CCNNGG 5 cut(s) 238, 929, 930, 1577, 2783
BsaWI WCCGGW 2 cut(s) 557, 2000
BsaXI ACNNNNNCTCC 2 cut(s) 2335, 2365
Bse118I RCCGGY 2 cut(s) 557, 2000
Bse21I CCTNAGG 3 cut(s) 498, 714, 786
Bse3DI GCAATG 2 cut(s) 1545, 1908
Bse8I GATNNNNATC 1 cut(s) 1594
BseBI CCWGG 6 cut(s) 931, 1331, 2310, 2405, 2558, 2937
BseDI CCNNGG 5 cut(s) 238, 929, 930, 1577, 2783
BseJI GATNNNNATC 1 cut(s) 1594
BseMI GCAATG 2 cut(s) 1545, 1908
BseMII CTCAG 9 cut(s) 269, 489, 951, 1428, 1705, 1748, 1924, 1939, 2553
BseRI GAGGAG 2 cut(s) 901, 2208
BseXI GCAGC 8 cut(s) 943, 1437, 1808, 2033, 2130, 2258, 2715, 2731
BseYI CCCAGC 4 cut(s) 611, 730, 1729, 2012
BsgI GTGCAG 1 cut(s) 2185
BshFI GGCC 3 cut(s) 417, 2152, 2716
BshTI ACCGGT 2 cut(s) 557, 2000
BsiHKCI CYCGRG 1 cut(s) 1508
BsiSI CCGG 3 cut(s) 558, 2001, 2527
BslFI GGGAC 1 cut(s) 829
BsmAI GTCTC 2 cut(s) 2296, 3032
BsmBI CGTCTC 1 cut(s) 2296
BsmFI GGGAC 1 cut(s) 829
BsmI GAATGC 2 cut(s) 625, 1306
BsnI GGCC 3 cut(s) 417, 2152, 2716
BsoBI CYCGRG 1 cut(s) 1508
Bsp119I TTCGAA 2 cut(s) 1707, 2838
Bsp1286I GDGCHC 1 cut(s) 284
Bsp143I GATC 9 cut(s) 178, 319, 745, 996, 1255, 1342, 2075, 2388, 2915
BspACI CCGC 4 cut(s) 47, 192, 1649, 1929
BspANI GGCC 3 cut(s) 417, 2152, 2716
BspCNI CTCAG 9 cut(s) 270, 490, 952, 1429, 1704, 1749, 1923, 1938, 2554
BspHI TCATGA 4 cut(s) 2298, 2428, 2727, 2770
BspLI GGNNCC 4 cut(s) 274, 922, 2322, 2635
BspMI ACCTGC 1 cut(s) 433
BspPI GGATC 4 cut(s) 991, 2083, 2396, 2923
BspT104I TTCGAA 2 cut(s) 1707, 2838
BsrDI GCAATG 2 cut(s) 1545, 1908
BsrFI RCCGGY 2 cut(s) 557, 2000
BssAI RCCGGY 2 cut(s) 557, 2000
BssECI CCNNGG 5 cut(s) 238, 929, 930, 1577, 2783
BssMI GATC 9 cut(s) 178, 319, 745, 996, 1255, 1342, 2075, 2388, 2915
BssNI GRCGYC 1 cut(s) 2305
BssSI CACGAG 1 cut(s) 842
BssT1I CCWWGG 2 cut(s) 238, 1577
Bst2BI CACGAG 1 cut(s) 842
Bst2UI CCWGG 6 cut(s) 931, 1331, 2310, 2405, 2558, 2937
Bst4CI ACNGT 8 cut(s) 1038, 1185, 1238, 1379, 2320, 2467, 2552, 2798
Bst6I CTCTTC 2 cut(s) 125, 498
BstACI GRCGYC 1 cut(s) 2305
BstBI TTCGAA 2 cut(s) 1707, 2838
BstC8I GCNNGC 1 cut(s) 1892
BstENI CCTNNNNNAGG 1 cut(s) 2408
BstKTI GATC 9 cut(s) 181, 322, 748, 999, 1258, 1345, 2078, 2391, 2918
BstMAI GTCTC 2 cut(s) 2296, 3032
BstMBI GATC 9 cut(s) 178, 319, 745, 996, 1255, 1342, 2075, 2388, 2915
BstMWI GCNNNNNNNGC 8 cut(s) 727, 1648, 1726, 1735, 1750, 1759, 2043, 2741
BstNI CCWGG 6 cut(s) 931, 1331, 2310, 2405, 2558, 2937
BstPAI GACNNNNGTC 1 cut(s) 1692
BstSCI CCNGG 7 cut(s) 929, 1329, 2308, 2403, 2526, 2556, 2935
BstSFI CTRYAG 1 cut(s) 901
BstV1I GCAGC 8 cut(s) 943, 1437, 1808, 2033, 2130, 2258, 2715, 2731
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 3 cut(s) 745, 996, 2075
BstYI RGATCY 3 cut(s) 745, 996, 2075
Bsu36I CCTNAGG 3 cut(s) 498, 714, 786
BsuI GTATCC 2 cut(s) 2206, 2335
BsuRI GGCC 3 cut(s) 417, 2152, 2716
BtrI CACGTC 1 cut(s) 84
BtsI GCAGTG 1 cut(s) 2173
BtsIMutI CAGTG 5 cut(s) 230, 521, 1491, 1635, 2173
BveI ACCTGC 1 cut(s) 433
Cac8I GCNNGC 1 cut(s) 1892
CciI TCATGA 4 cut(s) 2298, 2428, 2727, 2770
Cfr10I RCCGGY 2 cut(s) 557, 2000
Cfr13I GGNCC 3 cut(s) 235, 488, 2150
CsiI ACCWGGT 1 cut(s) 2403
Csp6I GTAC 4 cut(s) 895, 1039, 1294, 2656
CspAI ACCGGT 2 cut(s) 557, 2000
CspCI CAANNNNNGTGG 2 cut(s) 1854, 1889
CviQI GTAC 4 cut(s) 895, 1039, 1294, 2656
DpnI GATC 9 cut(s) 180, 321, 747, 998, 1257, 1344, 2077, 2390, 2917
DpnII GATC 9 cut(s) 178, 319, 745, 996, 1255, 1342, 2075, 2388, 2915
DrdI GACNNNNNNGTC 1 cut(s) 2310
DseDI GACNNNNNNGTC 1 cut(s) 2310
EaeI YGGCCR 2 cut(s) 415, 2714
Eam1104I CTCTTC 2 cut(s) 125, 498
EarI CTCTTC 2 cut(s) 125, 498
EciI GGCGGA 1 cut(s) 1638
Eco130I CCWWGG 2 cut(s) 238, 1577
Eco24I GRGCYC 1 cut(s) 284
Eco32I GATATC 1 cut(s) 1592
Eco47I GGWCC 2 cut(s) 235, 488
Eco57I CTGAAG 4 cut(s) 123, 146, 1661, 2769
Eco81I CCTNAGG 3 cut(s) 498, 714, 786
Eco88I CYCGRG 1 cut(s) 1508
EcoNI CCTNNNNNAGG 1 cut(s) 2408
EcoRI GAATTC 2 cut(s) 97, 1970
EcoRII CCWGG 6 cut(s) 929, 1329, 2308, 2403, 2556, 2935
EcoRV GATATC 1 cut(s) 1592
EcoT14I CCWWGG 2 cut(s) 238, 1577
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 2 cut(s) 238, 1577
Esp3I CGTCTC 1 cut(s) 2296
FalI AAGNNNNNCTT 8 cut(s) 13, 45, 2440, 2472, 2657, 2689, 2933, 2965
FaqI GGGAC 1 cut(s) 829
FauI CCCGC 2 cut(s) 40, 1922
FauNDI CATATG 3 cut(s) 1003, 1147, 2920
FbaI TGATCA 1 cut(s) 178
FblI GTMKAC 1 cut(s) 1240
Fnu4HI GCNGC 8 cut(s) 957, 1426, 1797, 2047, 2119, 2247, 2704, 2745
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 8 cut(s) 957, 1426, 1797, 2047, 2119, 2247, 2704, 2745
FspBI CTAG 4 cut(s) 285, 1578, 1802, 2825
GluI GCNGC 8 cut(s) 957, 1426, 1797, 2047, 2119, 2247, 2704, 2745
GsaI CCCAGC 4 cut(s) 615, 734, 1733, 2016
GsuI CTGGAG 4 cut(s) 363, 843, 1352, 1386
HaeIII GGCC 3 cut(s) 417, 2152, 2716
HapII CCGG 3 cut(s) 558, 2001, 2527
Hin1I GRCGYC 1 cut(s) 2305
HincII GTYRAC 2 cut(s) 1241, 1663
HindII GTYRAC 2 cut(s) 1241, 1663
HindIII AAGCTT 6 cut(s) 1030, 1106, 1888, 2035, 2513, 2942
HpaI GTTAAC 1 cut(s) 1663
HpaII CCGG 3 cut(s) 558, 2001, 2527
HphI GGTGA 9 cut(s) 296, 1004, 1118, 1465, 1868, 2015, 2690, 2705, 2864
Hpy166II GTNNAC 9 cut(s) 299, 411, 1241, 1456, 1491, 1663, 2470, 2794, 2957
Hpy8I GTNNAC 9 cut(s) 299, 411, 1241, 1456, 1491, 1663, 2470, 2794, 2957
HpyAV CCTTC 7 cut(s) 98, 1127, 1443, 1985, 2334, 2442, 2744
HpyCH4III ACNGT 8 cut(s) 1038, 1185, 1238, 1379, 2320, 2467, 2552, 2798
HpyCH4IV ACGT 3 cut(s) 83, 2305, 2393
HpyF10VI GCNNNNNNNGC 8 cut(s) 727, 1648, 1726, 1735, 1750, 1759, 2043, 2741
HpySE526I ACGT 3 cut(s) 83, 2305, 2393
Hsp92I GRCGYC 1 cut(s) 2305
Ksp22I TGATCA 1 cut(s) 178
KspAI GTTAAC 1 cut(s) 1663
Kzo9I GATC 9 cut(s) 178, 319, 745, 996, 1255, 1342, 2075, 2388, 2915
LmnI GCTCC 7 cut(s) 718, 735, 790, 1750, 2453, 2741, 2938
Lsp1109I GCAGC 8 cut(s) 943, 1437, 1808, 2033, 2130, 2258, 2715, 2731
LweI GCATC 4 cut(s) 335, 717, 1662, 2599
MabI ACCWGGT 1 cut(s) 2403
MaeI CTAG 4 cut(s) 285, 1578, 1802, 2825
MaeII ACGT 3 cut(s) 83, 2305, 2393
MaeIII GTNAC 6 cut(s) 598, 708, 1085, 1179, 1606, 2852
MalI GATC 9 cut(s) 180, 321, 747, 998, 1257, 1344, 2077, 2390, 2917
MboI GATC 9 cut(s) 178, 319, 745, 996, 1255, 1342, 2075, 2388, 2915
MboII GAAGA 4 cut(s) 139, 142, 485, 510
MfeI CAATTG 1 cut(s) 1601
MflI RGATCY 3 cut(s) 745, 996, 2075
MhlI GDGCHC 1 cut(s) 284
MlsI TGGCCA 2 cut(s) 417, 2716
MluNI TGGCCA 2 cut(s) 417, 2716
MlyI GAGTC 1 cut(s) 1343
MmeI TCCRAC 8 cut(s) 146, 1385, 1387, 1455, 1676, 1728, 2425, 2661
Mox20I TGGCCA 2 cut(s) 417, 2716
MroXI GAANNNNTTC 5 cut(s) 946, 1487, 1703, 1974, 2685
MscI TGGCCA 2 cut(s) 417, 2716
MseI TTAA 9 cut(s) 452, 1010, 1122, 1515, 1662, 1917, 2039, 2508, 2570
MslI CAYNNNNRTG 2 cut(s) 1064, 2850
Msp20I TGGCCA 2 cut(s) 417, 2716
MspA1I CMGCKG 3 cut(s) 644, 1929, 2249
MspI CCGG 3 cut(s) 558, 2001, 2527
MspR9I CCNGG 7 cut(s) 931, 1331, 2310, 2405, 2528, 2558, 2937
MunI CAATTG 1 cut(s) 1601
Mva1269I GAATGC 2 cut(s) 625, 1306
MvaI CCWGG 6 cut(s) 931, 1331, 2310, 2405, 2558, 2937
MwoI GCNNNNNNNGC 8 cut(s) 727, 1648, 1726, 1735, 1750, 1759, 2043, 2741
NciI CCSGG 1 cut(s) 2528
NdeI CATATG 3 cut(s) 1003, 1147, 2920
NdeII GATC 9 cut(s) 178, 319, 745, 996, 1255, 1342, 2075, 2388, 2915
NlaIV GGNNCC 4 cut(s) 274, 922, 2322, 2635
NmuCI GTSAC 1 cut(s) 2852
NspV TTCGAA 2 cut(s) 1707, 2838
OliI CACNNNNGTG 1 cut(s) 1064
PagI TCATGA 4 cut(s) 2298, 2428, 2727, 2770
PasI CCCWGGG 1 cut(s) 930
PctI GAATGC 2 cut(s) 625, 1306
PdmI GAANNNNTTC 5 cut(s) 946, 1487, 1703, 1974, 2685
PflFI GACNNNGTC 1 cut(s) 1236
PflMI CCANNNNNTGG 4 cut(s) 866, 1949, 2107, 2404
PinAI ACCGGT 2 cut(s) 557, 2000
PkrI GCNGC 8 cut(s) 958, 1427, 1798, 2048, 2120, 2248, 2705, 2746
PleI GAGTC 1 cut(s) 1342
PpsI GAGTC 1 cut(s) 1342
PshAI GACNNNNGTC 1 cut(s) 1692
PshBI ATTAAT 1 cut(s) 1122
Psp6I CCWGG 6 cut(s) 929, 1329, 2308, 2403, 2556, 2935
PspFI CCCAGC 4 cut(s) 611, 730, 1729, 2012
PspGI CCWGG 6 cut(s) 929, 1329, 2308, 2403, 2556, 2935
PspN4I GGNNCC 4 cut(s) 274, 922, 2322, 2635
PspPI GGNCC 3 cut(s) 235, 488, 2150
PsuI RGATCY 3 cut(s) 745, 996, 2075
PsyI GACNNNGTC 1 cut(s) 1236
PvuII CAGCTG 2 cut(s) 644, 2249
RsaI GTAC 4 cut(s) 896, 1040, 1295, 2657
RsaNI GTAC 4 cut(s) 895, 1039, 1294, 2656
RseI CAYNNNNRTG 2 cut(s) 1064, 2850
SalI GTCGAC 1 cut(s) 1239
SaqAI TTAA 9 cut(s) 452, 1010, 1122, 1515, 1662, 1917, 2039, 2508, 2570
SatI GCNGC 8 cut(s) 957, 1426, 1797, 2047, 2119, 2247, 2704, 2745
Sau3AI GATC 9 cut(s) 178, 319, 745, 996, 1255, 1342, 2075, 2388, 2915
Sau96I GGNCC 3 cut(s) 235, 488, 2150
SchI GAGTC 1 cut(s) 1343
ScrFI CCNGG 7 cut(s) 931, 1331, 2310, 2405, 2528, 2558, 2937
SduI GDGCHC 1 cut(s) 284
SexAI ACCWGGT 1 cut(s) 2403
SfaNI GCATC 4 cut(s) 335, 717, 1662, 2599
SfcI CTRYAG 1 cut(s) 901
SfuI TTCGAA 2 cut(s) 1707, 2838
SinI GGWCC 2 cut(s) 235, 488
SmiMI CAYNNNNRTG 2 cut(s) 1064, 2850
SmlI CTYRAG 3 cut(s) 373, 875, 1090
SmoI CTYRAG 3 cut(s) 373, 875, 1090
SsiI CCGC 4 cut(s) 47, 192, 1649, 1929
SspI AATATT 5 cut(s) 553, 1160, 2599, 2818, 2971
SspMI CTAG 4 cut(s) 285, 1578, 1802, 2825
StyD4I CCNGG 7 cut(s) 929, 1329, 2308, 2403, 2526, 2556, 2935
StyI CCWWGG 2 cut(s) 238, 1577
TaaI ACNGT 8 cut(s) 1038, 1185, 1238, 1379, 2320, 2467, 2552, 2798
TaiI ACGT 3 cut(s) 86, 2308, 2396
TaqI TCGA 5 cut(s) 607, 1191, 1240, 1707, 2838
TatI WGTACW 1 cut(s) 2655
Tru1I TTAA 9 cut(s) 452, 1010, 1122, 1515, 1662, 1917, 2039, 2508, 2570
Tru9I TTAA 9 cut(s) 452, 1010, 1122, 1515, 1662, 1917, 2039, 2508, 2570
TscAI CASTG 5 cut(s) 237, 528, 1498, 1642, 2173
TseFI GTSAC 1 cut(s) 2852
TseI GCWGC 8 cut(s) 956, 1425, 1796, 2046, 2118, 2246, 2703, 2744
Tsp45I GTSAC 1 cut(s) 2852
TspGWI ACGGA 1 cut(s) 1281
TspRI CASTG 5 cut(s) 237, 528, 1498, 1642, 2173
Tth111I GACNNNGTC 1 cut(s) 1236
Van91I CCANNNNNTGG 4 cut(s) 866, 1949, 2107, 2404
VpaK11BI GGWCC 2 cut(s) 235, 488
VspI ATTAAT 1 cut(s) 1122
XagI CCTNNNNNAGG 1 cut(s) 2408
XmaJI CCTAGG 1 cut(s) 1577
XmiI GTMKAC 1 cut(s) 1240
XmnI GAANNNNTTC 5 cut(s) 946, 1487, 1703, 1974, 2685
XspI CTAG 4 cut(s) 285, 1578, 1802, 2825
ZraI GACGTC 1 cut(s) 2306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.