Rh4BG394800

Xylosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0021397
Physical Location & Seq
Forward (+)
54598577 .. 54599011
435 bp
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UTR
Exon/CDS
Intron
Rh4BG394800.1

Sequence Viewer

Length: 435 bp
ATGTACTTTACGAATGTGATTCTATCGGAGGAAGGATATTTCCACTCTGTTATATGCAATTCACCAGAGTTTAGGAACACTACTGTGAACAGTGATTTAAGATACATGATTTGGGACACTCCCCCGAAAATGGAACCCCACTTCCTCAATACATCAGTTTATGATCAAATGGTTGAAAGTGGAGCTGCTTTTGGCCGACAATTCCAGAAGGACGACCCTGTGCTGGACATGGTTGATGAAAAGATCCTCAAGCGCGGGCGAAATCGAGCTGCCCCAGGTGCATGGTGCTCTGGCTGGAAGAGCTGGTGGATGGATCCTTGCTCTCAATGGGGGGATGCCAATATCTTGAAGCCTGGACCCCAAGCAAAGAAATTTGAAGAATCCATTACTAACCTTCTTGATGACTGGAGCTCACAATCAAATCAGTGTAAATGA

Protein Analysis

144

Amino Acids

16.64

Weight (kDa)

5.13

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 1 - 57 1.2e-08 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021397)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0395711
rosa_multiflora Rmu_co8443505.1_g000001
rosa_samantha Rh4BG394800 Rh4CG409700 Rh4DG053300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 255
AciI CCGC 1 cut(s) 255
AclWI GGATC 3 cut(s) 238, 308, 321
AcoI YGGCCR 1 cut(s) 193
AcsI RAATTY 1 cut(s) 371
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 130, 223
AgsI TTSAA 3 cut(s) 176, 349, 377
AjnI CCWGG 2 cut(s) 274, 352
AleI CACNNNNGTG 1 cut(s) 83
AloI GAACNNNNNNTCC 2 cut(s) 126, 158
AluBI AGCT 4 cut(s) 185, 269, 303, 411
AluI AGCT 4 cut(s) 185, 269, 303, 411
Alw21I GWGCWC 2 cut(s) 290, 413
AlwI GGATC 3 cut(s) 238, 308, 321
AoxI GGCC 1 cut(s) 193
ApeKI GCWGC 2 cut(s) 185, 269
ApoI RAATTY 1 cut(s) 371
AspLEI GCGC 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 356
AsuHPI GGTGA 1 cut(s) 54
AvaII GGWCC 1 cut(s) 356
BamHI GGATCC 1 cut(s) 313
BanII GRGCYC 1 cut(s) 413
Bbv12I GWGCWC 2 cut(s) 290, 413
BbvI GCAGC 2 cut(s) 172, 256
BccI CCATC 1 cut(s) 304
BciT130I CCWGG 2 cut(s) 276, 354
BclI TGATCA 1 cut(s) 163
BisI GCNGC 2 cut(s) 186, 270
BlsI GCNGC 2 cut(s) 187, 271
Bme1390I CCNGG 2 cut(s) 276, 354
Bme18I GGWCC 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 3 cut(s) 135, 315, 358
BmrFI CCNGG 2 cut(s) 276, 354
BmsI GCATC 1 cut(s) 325
BpmI CTGGAG 1 cut(s) 427
BpuEI CTTGAG 1 cut(s) 233
BsaJI CCNNGG 1 cut(s) 274
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 223
Bse1I ACTGG 1 cut(s) 410
BseBI CCWGG 2 cut(s) 276, 354
BseDI CCNNGG 1 cut(s) 274
BseGI GGATG 2 cut(s) 315, 340
BseLI CCNNNNNNNGG 2 cut(s) 130, 223
BseNI ACTGG 1 cut(s) 410
BseXI GCAGC 2 cut(s) 172, 256
Bsh1236I CGCG 1 cut(s) 255
BshFI GGCC 1 cut(s) 195
BsiHKAI GWGCWC 2 cut(s) 290, 413
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 2 cut(s) 130, 223
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 1 cut(s) 195
Bsp1286I GDGCHC 2 cut(s) 290, 413
Bsp143I GATC 3 cut(s) 163, 243, 313
BspACI CCGC 1 cut(s) 255
BspANI GGCC 1 cut(s) 195
BspFNI CGCG 1 cut(s) 255
BspLI GGNNCC 3 cut(s) 135, 315, 358
BspPI GGATC 3 cut(s) 238, 308, 321
BspQI GCTCTTC 1 cut(s) 293
BsrI ACTGG 1 cut(s) 410
BssECI CCNNGG 1 cut(s) 274
BssMI GATC 3 cut(s) 163, 243, 313
Bst2UI CCWGG 2 cut(s) 276, 354
Bst4CI ACNGT 2 cut(s) 85, 92
Bst6I CTCTTC 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 257
BstF5I GGATG 2 cut(s) 315, 340
BstFNI CGCG 1 cut(s) 255
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 3 cut(s) 166, 246, 316
BstMBI GATC 3 cut(s) 163, 243, 313
BstMWI GCNNNNNNNGC 2 cut(s) 278, 300
BstNI CCWGG 2 cut(s) 276, 354
BstSCI CCNGG 2 cut(s) 274, 352
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 2 cut(s) 172, 256
BstX2I RGATCY 2 cut(s) 243, 313
BstXI CCANNNNNNTGG 1 cut(s) 282
BstYI RGATCY 2 cut(s) 243, 313
BsuRI GGCC 1 cut(s) 195
BtsCI GGATG 2 cut(s) 315, 340
BtsIMutI CAGTG 2 cut(s) 97, 431
Cac8I GCNNGC 1 cut(s) 257
CfoI GCGC 1 cut(s) 255
Cfr13I GGNCC 1 cut(s) 356
Csp6I GTAC 1 cut(s) 4
CviAII CATG 3 cut(s) 106, 229, 282
CviJI RGCY 7 cut(s) 185, 195, 269, 294, 303, 352, 411
CviKI_1 RGCY 7 cut(s) 185, 195, 269, 294, 303, 352, 411
CviQI GTAC 1 cut(s) 4
DpnI GATC 3 cut(s) 165, 245, 315
DpnII GATC 3 cut(s) 163, 243, 313
EaeI YGGCCR 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Ecl136II GAGCTC 1 cut(s) 411
Eco24I GRGCYC 1 cut(s) 413
Eco47I GGWCC 1 cut(s) 356
Eco53kI GAGCTC 1 cut(s) 411
EcoICRI GAGCTC 1 cut(s) 411
EcoRII CCWGG 2 cut(s) 274, 352
EcoT38I GRGCYC 1 cut(s) 413
FaeI CATG 3 cut(s) 109, 232, 285
FaiI YATR 6 cut(s) 53, 55, 107, 162, 230, 283
FaqI GGGAC 1 cut(s) 128
FatI CATG 3 cut(s) 105, 228, 281
FauI CCCGC 1 cut(s) 248
FbaI TGATCA 1 cut(s) 163
Fnu4HI GCNGC 2 cut(s) 186, 270
FokI GGATG 2 cut(s) 322, 347
FriOI GRGCYC 1 cut(s) 413
Fsp4HI GCNGC 2 cut(s) 186, 270
GlaI GCGC 1 cut(s) 254
GluI GCNGC 2 cut(s) 186, 270
GsuI CTGGAG 1 cut(s) 427
HaeIII GGCC 1 cut(s) 195
HhaI GCGC 1 cut(s) 255
Hin1II CATG 3 cut(s) 109, 232, 285
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HinfI GANTC 2 cut(s) 19, 380
HphI GGTGA 1 cut(s) 54
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 1 cut(s) 28
Hpy188III TCNNGA 3 cut(s) 205, 346, 398
Hpy8I GTNNAC 1 cut(s) 88
HpyAV CCTTC 3 cut(s) 26, 202, 404
HpyCH4III ACNGT 2 cut(s) 85, 92
HpyCH4V TGCA 2 cut(s) 57, 281
HpyF10VI GCNNNNNNNGC 2 cut(s) 278, 300
Hsp92II CATG 3 cut(s) 109, 232, 285
HspAI GCGC 1 cut(s) 253
Ksp22I TGATCA 1 cut(s) 163
Kzo9I GATC 3 cut(s) 163, 243, 313
LguI GCTCTTC 1 cut(s) 293
LmnI GCTCC 2 cut(s) 182, 408
Lsp1109I GCAGC 2 cut(s) 172, 256
LweI GCATC 1 cut(s) 325
MalI GATC 3 cut(s) 165, 245, 315
MboI GATC 3 cut(s) 163, 243, 313
MboII GAAGA 2 cut(s) 310, 389
MflI RGATCY 2 cut(s) 243, 313
MhlI GDGCHC 2 cut(s) 290, 413
MluCI AATT 3 cut(s) 58, 200, 371
MnlI CCTC 3 cut(s) 22, 155, 257
MseI TTAA 1 cut(s) 98
MslI CAYNNNNRTG 1 cut(s) 83
MspR9I CCNGG 2 cut(s) 276, 354
MvaI CCWGG 2 cut(s) 276, 354
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 2 cut(s) 278, 300
NdeII GATC 3 cut(s) 163, 243, 313
NlaIII CATG 3 cut(s) 109, 232, 285
NlaIV GGNNCC 3 cut(s) 135, 315, 358
OliI CACNNNNGTG 1 cut(s) 83
PciSI GCTCTTC 1 cut(s) 293
PfeI GAWTC 2 cut(s) 19, 380
PkrI GCNGC 2 cut(s) 187, 271
Psp124BI GAGCTC 1 cut(s) 413
Psp6I CCWGG 2 cut(s) 274, 352
PspGI CCWGG 2 cut(s) 274, 352
PspN4I GGNNCC 3 cut(s) 135, 315, 358
PspPI GGNCC 1 cut(s) 356
PsuI RGATCY 2 cut(s) 243, 313
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 1 cut(s) 83
SacI GAGCTC 1 cut(s) 413
SapI GCTCTTC 1 cut(s) 293
SaqAI TTAA 1 cut(s) 98
SatI GCNGC 2 cut(s) 186, 270
Sau3AI GATC 3 cut(s) 163, 243, 313
Sau96I GGNCC 1 cut(s) 356
ScrFI CCNGG 2 cut(s) 276, 354
SduI GDGCHC 2 cut(s) 290, 413
SetI ASST 6 cut(s) 187, 271, 280, 305, 396, 413
SfaNI GCATC 1 cut(s) 325
SinI GGWCC 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 3 cut(s) 58, 200, 371
SsiI CCGC 1 cut(s) 255
SstI GAGCTC 1 cut(s) 413
StyD4I CCNGG 2 cut(s) 274, 352
TaaI ACNGT 2 cut(s) 85, 92
TaqI TCGA 1 cut(s) 265
TasI AATT 3 cut(s) 58, 200, 371
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 2 cut(s) 19, 380
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TscAI CASTG 2 cut(s) 97, 431
TseI GCWGC 2 cut(s) 185, 269
TspDTI ATGAA 1 cut(s) 252
TspRI CASTG 2 cut(s) 97, 431
VpaK11BI GGWCC 1 cut(s) 356
XapI RAATTY 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.