Rh4DG273300

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0030092
Physical Location & Seq
Forward (+)
49314847 .. 49315137
291 bp
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UTR
Exon/CDS
Intron
Rh4DG273300.1

Sequence Viewer

Length: 291 bp
ATGGACGCAATCAGCCAATTACAAGAACAGGTGAACAAAATCGCGACCATTGCGTTTACCACCATCGGAACCCTAGAGAGGGACGATCCACCCGTCCGAATCTCCCCCAATTACCCCGACTTCGAGTCAGCACCACCAACCAACCCGAACCCGACTGACGATGCCACTTATTTTGCGATGCAACCCAAGCTCATGAGCGCGGATCTAGTCAAAGCAGCCAAGCAGGTCTCAACCTTTAATCAATTGGATTTGGGTCGAATAACAAAGCCACTGGCCGTAATGTTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med21

Protein Analysis

96

Amino Acids

10.58

Weight (kDa)

4.62

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med21 PF11221 1 - 78 2.2e-11 Subunit 21 of Mediator complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0030092)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0426791
rosa_samantha Rh4DG273300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 214
AccII CGCG 2 cut(s) 44, 200
AciI CCGC 1 cut(s) 200
AclWI GGATC 2 cut(s) 80, 210
AcoI YGGCCR 1 cut(s) 273
AfiI CCNNNNNNNGG 2 cut(s) 78, 79
AhdI GACNNNNNGTC 1 cut(s) 124
AluBI AGCT 1 cut(s) 190
AluI AGCT 1 cut(s) 190
Alw26I GTCTC 1 cut(s) 232
AlwI GGATC 2 cut(s) 80, 210
AoxI GGCC 1 cut(s) 273
ApeKI GCWGC 1 cut(s) 215
AspLEI GCGC 1 cut(s) 200
AsuHPI GGTGA 1 cut(s) 43
BbvI GCAGC 1 cut(s) 227
BccI CCATC 1 cut(s) 71
BceAI ACGGC 1 cut(s) 260
BcoDI GTCTC 1 cut(s) 232
BfaI CTAG 2 cut(s) 74, 206
BfuAI ACCTGC 1 cut(s) 214
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
BmeRI GACNNNNNGTC 1 cut(s) 124
BmiI GGNNCC 1 cut(s) 70
BmsI GCATC 2 cut(s) 151, 168
BsaI GGTCTC 1 cut(s) 232
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 79
Bse1I ACTGG 1 cut(s) 276
Bse3DI GCAATG 1 cut(s) 48
BseLI CCNNNNNNNGG 2 cut(s) 78, 79
BseMI GCAATG 1 cut(s) 48
BseNI ACTGG 1 cut(s) 276
BseXI GCAGC 1 cut(s) 227
Bsh1236I CGCG 2 cut(s) 44, 200
BshFI GGCC 1 cut(s) 275
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 78, 79
BsmAI GTCTC 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 1 cut(s) 275
Bso31I GGTCTC 1 cut(s) 232
Bsp143I GATC 2 cut(s) 85, 202
Bsp68I TCGCGA 1 cut(s) 44
BspACI CCGC 1 cut(s) 200
BspANI GGCC 1 cut(s) 275
BspFNI CGCG 2 cut(s) 44, 200
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 70
BspMI ACCTGC 1 cut(s) 214
BspPI GGATC 2 cut(s) 80, 210
BspTNI GGTCTC 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 48
BsrI ACTGG 1 cut(s) 276
BssMI GATC 2 cut(s) 85, 202
BstFNI CGCG 2 cut(s) 44, 200
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 2 cut(s) 88, 205
BstMAI GTCTC 1 cut(s) 232
BstMBI GATC 2 cut(s) 85, 202
BstMWI GCNNNNNNNGC 2 cut(s) 50, 187
BstUI CGCG 2 cut(s) 44, 200
BstV1I GCAGC 1 cut(s) 227
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BsuRI GGCC 1 cut(s) 275
BtgZI GCGATG 1 cut(s) 191
BtsIMutI CAGTG 1 cut(s) 269
BtuMI TCGCGA 1 cut(s) 44
BveI ACCTGC 1 cut(s) 214
CciI TCATGA 1 cut(s) 192
CfoI GCGC 1 cut(s) 200
CseI GACGC 1 cut(s) 14
CspCI CAANNNNNGTGG 2 cut(s) 154, 189
CviAII CATG 1 cut(s) 193
CviJI RGCY 5 cut(s) 15, 190, 218, 268, 275
CviKI_1 RGCY 5 cut(s) 15, 190, 218, 268, 275
DpnI GATC 2 cut(s) 87, 204
DpnII GATC 2 cut(s) 85, 202
DriI GACNNNNNGTC 1 cut(s) 124
EaeI YGGCCR 1 cut(s) 273
Eam1105I GACNNNNNGTC 1 cut(s) 124
Eco31I GGTCTC 1 cut(s) 232
FaeI CATG 1 cut(s) 196
FaiI YATR 1 cut(s) 194
FaqI GGGAC 1 cut(s) 95
FatI CATG 1 cut(s) 192
Fnu4HI GCNGC 1 cut(s) 216
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 2 cut(s) 74, 206
GlaI GCGC 1 cut(s) 199
GluI GCNGC 1 cut(s) 216
HaeIII GGCC 1 cut(s) 275
HgaI GACGC 1 cut(s) 14
HhaI GCGC 1 cut(s) 200
Hin1II CATG 1 cut(s) 196
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HinfI GANTC 2 cut(s) 99, 125
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 2 cut(s) 34, 57
Hpy188I TCNGA 2 cut(s) 68, 98
Hpy188III TCNNGA 2 cut(s) 43, 193
Hpy8I GTNNAC 2 cut(s) 34, 57
HpyCH4V TGCA 1 cut(s) 181
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 187
Hsp92II CATG 1 cut(s) 196
HspAI GCGC 1 cut(s) 198
Kzo9I GATC 2 cut(s) 85, 202
LpnPI CCDG 3 cut(s) 14, 209, 257
Lsp1109I GCAGC 1 cut(s) 227
LweI GCATC 2 cut(s) 151, 168
MaeI CTAG 2 cut(s) 74, 206
MalI GATC 2 cut(s) 87, 204
MboI GATC 2 cut(s) 85, 202
MfeI CAATTG 1 cut(s) 242
MflI RGATCY 1 cut(s) 202
MluCI AATT 3 cut(s) 17, 109, 242
MlyI GAGTC 1 cut(s) 134
MnlI CCTC 1 cut(s) 72
MseI TTAA 1 cut(s) 237
MunI CAATTG 1 cut(s) 242
MvnI CGCG 2 cut(s) 44, 200
MwoI GCNNNNNNNGC 2 cut(s) 50, 187
NdeII GATC 2 cut(s) 85, 202
NlaIII CATG 1 cut(s) 196
NlaIV GGNNCC 1 cut(s) 70
NruI TCGCGA 1 cut(s) 44
PagI TCATGA 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PfeI GAWTC 1 cut(s) 99
PkrI GCNGC 1 cut(s) 217
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
PspN4I GGNNCC 1 cut(s) 70
PsuI RGATCY 1 cut(s) 202
RruI TCGCGA 1 cut(s) 44
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 2 cut(s) 85, 202
SchI GAGTC 1 cut(s) 134
SetI ASST 4 cut(s) 33, 192, 228, 236
SfaNI GCATC 2 cut(s) 151, 168
Sse9I AATT 3 cut(s) 17, 109, 242
SsiI CCGC 1 cut(s) 200
SspMI CTAG 2 cut(s) 74, 206
TaqI TCGA 2 cut(s) 123, 256
TasI AATT 3 cut(s) 17, 109, 242
TfiI GAWTC 1 cut(s) 99
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 1 cut(s) 276
TseI GCWGC 1 cut(s) 215
TspRI CASTG 1 cut(s) 276
XspI CTAG 2 cut(s) 74, 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.