Rh4DG427100

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0034477
Physical Location & Seq
Forward (+)
63280259 .. 63280555
297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG427100.1

Sequence Viewer

Length: 297 bp
ATGCAGTCCGACGGCGCCGTGGGAGGTTTCCGGCTCCGGTGGGACGTGTTCCTAAGCTTTAGAGGAGAGACACCCGCCACACCATCACCAACAACCTCTACAACGCGCTCGAACAACGCGGCGTACGAGTCTTCCGCGACGACGACGGCCTCAACCGCGGCGACGACATCGCGCCGAGTCTTCTGGAAGCGATCGAGGACTCGGCGGCGGCCGTCGTCGTGTTGTCGCCGAGATACGCCGACTCGAGGTGGTGCCTTGAGGAGCTCGCGAAGATCTGCGACGGCGGCCGGAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

98

Amino Acids

10.64

Weight (kDa)

11.85

Isoelectric Point (pI)

98.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0034477)

Species Orthologous Gene IDs
rosa_samantha Rh4CG445600 Rh4DG427100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 14, 251
AccII CGCG 6 cut(s) 106, 119, 137, 158, 172, 268
AciI CCGC 8 cut(s) 75, 119, 135, 156, 158, 205, 208, 284
AcoI YGGCCR 2 cut(s) 209, 285
AcyI GRCGYC 1 cut(s) 15
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 245
AflIII ACRYGT 1 cut(s) 45
AjiI CACGTC 1 cut(s) 46
AluBI AGCT 2 cut(s) 57, 264
AluI AGCT 2 cut(s) 57, 264
Alw21I GWGCWC 1 cut(s) 266
Alw26I GTCTC 2 cut(s) 62, 285
Ama87I CYCGRG 1 cut(s) 243
AoxI GGCC 3 cut(s) 147, 209, 285
AspLEI GCGC 3 cut(s) 17, 108, 174
AsuHPI GGTGA 1 cut(s) 78
AvaI CYCGRG 1 cut(s) 243
BanI GGYRCC 2 cut(s) 14, 251
BanII GRGCYC 1 cut(s) 266
BbsI GAAGAC 2 cut(s) 123, 172
Bbv12I GWGCWC 1 cut(s) 266
BccI CCATC 1 cut(s) 91
BceAI ACGGC 4 cut(s) 2, 28, 162, 196
BcoDI GTCTC 2 cut(s) 62, 285
BfoI RGCGCY 1 cut(s) 18
BglII AGATCT 1 cut(s) 272
BisI GCNGC 5 cut(s) 120, 159, 206, 209, 285
BlsI GCNGC 5 cut(s) 121, 160, 207, 210, 286
BmeT110I CYCGRG 1 cut(s) 243
BmgBI CACGTC 1 cut(s) 46
BmiI GGNNCC 3 cut(s) 16, 35, 253
BpiI GAAGAC 2 cut(s) 123, 172
Bpu10I CCTNAGC 1 cut(s) 53
BpuEI CTTGAG 1 cut(s) 277
BsaHI GRCGYC 1 cut(s) 15
BsaJI CCNNGG 2 cut(s) 18, 156
BsaWI WCCGGW 1 cut(s) 36
Bsc4I CCNNNNNNNGG 1 cut(s) 245
BseDI CCNNGG 2 cut(s) 18, 156
BseLI CCNNNNNNNGG 1 cut(s) 245
BseRI GAGGAG 2 cut(s) 78, 274
BseX3I CGGCCG 2 cut(s) 209, 285
Bsh1236I CGCG 6 cut(s) 106, 119, 137, 158, 172, 268
Bsh1285I CGRYCG 3 cut(s) 194, 212, 288
BshFI GGCC 3 cut(s) 149, 211, 287
BshNI GGYRCC 2 cut(s) 14, 251
BsiEI CGRYCG 3 cut(s) 194, 212, 288
BsiHKAI GWGCWC 1 cut(s) 266
BsiHKCI CYCGRG 1 cut(s) 243
BsiSI CCGG 3 cut(s) 31, 37, 288
BsiWI CGTACG 1 cut(s) 123
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 2 cut(s) 62, 285
BsmFI GGGAC 1 cut(s) 56
BsnI GGCC 3 cut(s) 149, 211, 287
BsoBI CYCGRG 1 cut(s) 243
Bsp1286I GDGCHC 1 cut(s) 266
Bsp143I GATC 2 cut(s) 191, 272
Bsp68I TCGCGA 1 cut(s) 268
BspACI CCGC 8 cut(s) 75, 119, 135, 156, 158, 205, 208, 284
BspANI GGCC 3 cut(s) 149, 211, 287
BspFNI CGCG 6 cut(s) 106, 119, 137, 158, 172, 268
BspLI GGNNCC 3 cut(s) 16, 35, 253
BspT107I GGYRCC 2 cut(s) 14, 251
BssECI CCNNGG 2 cut(s) 18, 156
BssMI GATC 2 cut(s) 191, 272
BssNI GRCGYC 1 cut(s) 15
BstACI GRCGYC 1 cut(s) 15
BstC8I GCNNGC 1 cut(s) 266
BstDEI CTNAG 1 cut(s) 53
BstDSI CCRYGG 2 cut(s) 18, 156
BstFNI CGCG 6 cut(s) 106, 119, 137, 158, 172, 268
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 3 cut(s) 17, 108, 174
BstKTI GATC 2 cut(s) 194, 275
BstMAI GTCTC 2 cut(s) 62, 285
BstMBI GATC 2 cut(s) 191, 272
BstMCI CGRYCG 3 cut(s) 194, 212, 288
BstMWI GCNNNNNNNGC 2 cut(s) 155, 284
BstUI CGCG 6 cut(s) 106, 119, 137, 158, 172, 268
BstV2I GAAGAC 2 cut(s) 123, 172
BstX2I RGATCY 1 cut(s) 272
BstYI RGATCY 1 cut(s) 272
BstZI CGGCCG 2 cut(s) 209, 285
BsuRI GGCC 3 cut(s) 149, 211, 287
BtgI CCRYGG 2 cut(s) 18, 156
BtgZI GCGATG 1 cut(s) 153
BtrI CACGTC 1 cut(s) 46
BtuMI TCGCGA 1 cut(s) 268
Cac8I GCNNGC 1 cut(s) 266
CfoI GCGC 3 cut(s) 17, 108, 174
Cfr42I CCGCGG 1 cut(s) 159
Csp6I GTAC 1 cut(s) 124
CviJI RGCY 6 cut(s) 34, 57, 149, 211, 264, 287
CviKI_1 RGCY 6 cut(s) 34, 57, 149, 211, 264, 287
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 1 cut(s) 53
DinI GGCGCC 1 cut(s) 16
DpnI GATC 2 cut(s) 193, 274
DpnII GATC 2 cut(s) 191, 272
EaeI YGGCCR 2 cut(s) 209, 285
EagI CGGCCG 2 cut(s) 209, 285
Ecl136II GAGCTC 1 cut(s) 264
EclXI CGGCCG 2 cut(s) 209, 285
Eco24I GRGCYC 1 cut(s) 266
Eco52I CGGCCG 2 cut(s) 209, 285
Eco53kI GAGCTC 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 243
EcoICRI GAGCTC 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 266
EgeI GGCGCC 1 cut(s) 16
EheI GGCGCC 1 cut(s) 16
FaqI GGGAC 1 cut(s) 56
FauI CCCGC 1 cut(s) 82
Fnu4HI GCNGC 5 cut(s) 120, 159, 206, 209, 285
FriOI GRGCYC 1 cut(s) 266
Fsp4HI GCNGC 5 cut(s) 120, 159, 206, 209, 285
GlaI GCGC 3 cut(s) 16, 107, 173
GluI GCNGC 5 cut(s) 120, 159, 206, 209, 285
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 3 cut(s) 149, 211, 287
HapII CCGG 3 cut(s) 31, 37, 288
HhaI GCGC 3 cut(s) 17, 108, 174
Hin1I GRCGYC 1 cut(s) 15
Hin6I GCGC 3 cut(s) 15, 106, 172
HinP1I GCGC 3 cut(s) 15, 106, 172
HindIII AAGCTT 1 cut(s) 55
HinfI GANTC 4 cut(s) 128, 177, 199, 241
HpaII CCGG 3 cut(s) 31, 37, 288
HphI GGTGA 1 cut(s) 78
Hpy188I TCNGA 1 cut(s) 10
Hpy188III TCNNGA 2 cut(s) 184, 267
Hpy99I CGWCG 8 cut(s) 14, 142, 145, 148, 166, 217, 220, 283
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 284
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 1 cut(s) 15
HspAI GCGC 3 cut(s) 15, 106, 172
KasI GGCGCC 1 cut(s) 14
KspI CCGCGG 1 cut(s) 159
Kzo9I GATC 2 cut(s) 191, 272
LmnI GCTCC 2 cut(s) 39, 261
LpnPI CCDG 3 cut(s) 44, 50, 169
MaeII ACGT 1 cut(s) 45
MalI GATC 2 cut(s) 193, 274
MboI GATC 2 cut(s) 191, 272
MboII GAAGA 3 cut(s) 123, 172, 282
MflI RGATCY 1 cut(s) 272
MhlI GDGCHC 1 cut(s) 266
Mly113I GGCGCC 1 cut(s) 15
MlyI GAGTC 4 cut(s) 137, 186, 193, 235
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 7 cut(s) 17, 56, 106, 160, 189, 239, 252
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 3 cut(s) 31, 37, 288
MvnI CGCG 6 cut(s) 106, 119, 137, 158, 172, 268
MwoI GCNNNNNNNGC 2 cut(s) 155, 284
NarI GGCGCC 1 cut(s) 15
NdeII GATC 2 cut(s) 191, 272
NlaIV GGNNCC 3 cut(s) 16, 35, 253
NmeAIII GCCGAG 3 cut(s) 181, 200, 254
NruI TCGCGA 1 cut(s) 268
PaeR7I CTCGAG 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 123
Pfl23II CGTACG 1 cut(s) 123
PkrI GCNGC 5 cut(s) 121, 160, 207, 210, 286
Ple19I CGATCG 1 cut(s) 194
PleI GAGTC 4 cut(s) 136, 185, 193, 235
PluTI GGCGCC 1 cut(s) 18
PpsI GAGTC 4 cut(s) 136, 185, 193, 235
Psp124BI GAGCTC 1 cut(s) 266
PspLI CGTACG 1 cut(s) 123
PspN4I GGNNCC 3 cut(s) 16, 35, 253
PspXI VCTCGAGB 1 cut(s) 243
PsuI RGATCY 1 cut(s) 272
PvuI CGATCG 1 cut(s) 194
RruI TCGCGA 1 cut(s) 268
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SacI GAGCTC 1 cut(s) 266
SacII CCGCGG 1 cut(s) 159
SatI GCNGC 5 cut(s) 120, 159, 206, 209, 285
Sau3AI GATC 2 cut(s) 191, 272
SchI GAGTC 4 cut(s) 137, 186, 193, 235
SduI GDGCHC 1 cut(s) 266
SetI ASST 6 cut(s) 28, 48, 59, 98, 250, 266
SfoI GGCGCC 1 cut(s) 16
Sfr274I CTCGAG 1 cut(s) 243
Sfr303I CCGCGG 1 cut(s) 159
SgrBI CCGCGG 1 cut(s) 159
SlaI CTCGAG 1 cut(s) 243
SmlI CTYRAG 2 cut(s) 243, 256
SmoI CTYRAG 2 cut(s) 243, 256
SsiI CCGC 8 cut(s) 75, 119, 135, 156, 158, 205, 208, 284
SspDI GGCGCC 1 cut(s) 14
SstI GAGCTC 1 cut(s) 266
TaiI ACGT 1 cut(s) 48
TaqI TCGA 3 cut(s) 110, 194, 244
TauI GCSGC 5 cut(s) 122, 161, 208, 211, 287
XhoI CTCGAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.