Rh5CG104500

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0034488
Physical Location & Seq
Forward (+)
8504297 .. 8509926
5630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG104500.1

Sequence Viewer

Length: 1002 bp
ATGGATTTGAAAACGACGCCGTTGCGGTTCACTCTGGGCAAGCAGTCGTCTCTGGCGCCGGAGCGTGAATCCGACGCCGGAGACGCGGAGGAGGACGAGGTGGAGGTGATCGATCCCCGAGTGAGGCTTATGTACTTGGCCAATGAAGCCGACTTGGACGGGATCAAGGAGCTTCTCGATTCCGGCATCGACGTCAATTTCCGCGACATCGATAATCGGACGGCGCTGCATGTCGCCGCGTGTCAGGGCTACTCCGACGTCGTTTCGCTGCTGCTCGAGCGCGGAGCTGATGTTAATACTAAAGATCGCTGGGGCAGCTCCCCTCTTGCAGATGCTATATACTATAAGAACCATGATGTGATCAAGCTTCTGGAGAAGCGCGGCGCGAAGCCTCTGATGGCACCAATGCGCGTTAAGTATGCAAGAGAAGTTCCCGAATATGAAATTGATCCCAAGGAGCTTGATTTTACTAACAGTGTTGAAATAACCAAGGGAACATTCCATTTAGCATCTTGGCGCGGAATACAGGTGGCTGTGAAAAGGCTTGCGGAGGAACTAATTGTTGACGAGGCTAAAGTGAAGGCATTTACGGATGAGCTTACATTGCTTCAAAAGATACGGCATCCAAATGTAGTGCAATTTCTGGGTGCAGTAACTCAAAGTAGTCCAATGATGATTGTAACAGAATACTTGCCTAAGGGAGACCTACGTGCATTTTTAAAAAGAAAAGGAGCATTGAAACCGTCGACAGCTGTGAGATTTGCACTTGATATTGCAAGGGGAATGAATTATTTGCATGAGAATAAACCAGCACCAATTATTCATCGTGATCTTGAGCCTTCAAATATATTGCGAGATGATTCTGGGACCATAAAAGTTGCTGATTTTGGAGTGAGCAAGTTGCTGACGGTTAAAGAAGATAAGCCTTTGATTTGTCAAGATGCTTCATGTGAGTGTTTCCTTCTGGGCAATACTTTATTGAATAGATCCTTACCAAACTAA

Protein Analysis

333

Amino Acids

37.23

Weight (kDa)

5.55

Isoelectric Point (pI)

33.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 43 - 130 6.4e-16 Ankyrin repeats (3 copies)
Ank_4 PF13637 43 - 92 1.4e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 58 - 109 2e-08 Ankyrin repeats (many copies)
Ank PF00023 71 - 103 5.9e-09 Ankyrin repeat
PK_Tyr_Ser-Thr PF07714 161 - 306 4.2e-35 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 172 - 307 1.2e-31 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0034488)

Species Orthologous Gene IDs
rosa_samantha Rh5CG104500 Rh5DG091100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 195, 261
AccB1I GGYRCC 2 cut(s) 55, 400
AccI GTMKAC 1 cut(s) 746
AccII CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
AciI CCGC 8 cut(s) 25, 86, 202, 237, 282, 381, 519, 548
AclWI GGATC 4 cut(s) 107, 170, 443, 981
AcoI YGGCCR 1 cut(s) 138
AcyI GRCGYC 5 cut(s) 17, 56, 75, 192, 258
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 6 cut(s) 10, 482, 611, 739, 843, 982
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AluBI AGCT 7 cut(s) 172, 287, 318, 367, 460, 598, 752
AluI AGCT 7 cut(s) 172, 287, 318, 367, 460, 598, 752
Alw26I GTCTC 3 cut(s) 54, 75, 696
AlwI GGATC 4 cut(s) 107, 170, 443, 981
Ama87I CYCGRG 2 cut(s) 117, 275
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 4 cut(s) 226, 268, 271, 315
AspLEI GCGC 7 cut(s) 58, 226, 282, 381, 386, 411, 519
AspS9I GGNCC 1 cut(s) 867
AsuHPI GGTGA 1 cut(s) 118
AvaI CYCGRG 2 cut(s) 117, 275
AvaII GGWCC 1 cut(s) 867
AxyI CCTNAGG 1 cut(s) 696
BalI TGGCCA 1 cut(s) 140
BanI GGYRCC 2 cut(s) 55, 400
BbvI GCAGC 4 cut(s) 213, 255, 258, 327
BccI CCATC 1 cut(s) 391
BceAI ACGGC 3 cut(s) 4, 237, 635
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BclI TGATCA 1 cut(s) 360
BcoDI GTCTC 3 cut(s) 54, 75, 696
BfoI RGCGCY 2 cut(s) 59, 227
BisI GCNGC 6 cut(s) 227, 237, 269, 272, 316, 382
BlsI GCNGC 6 cut(s) 228, 238, 270, 273, 317, 383
Bme18I GGWCC 1 cut(s) 867
BmeT110I CYCGRG 2 cut(s) 117, 275
BmgT120I GGNCC 1 cut(s) 867
BmiI GGNNCC 3 cut(s) 57, 402, 868
BmsI GCATC 5 cut(s) 195, 322, 518, 631, 931
BpmI CTGGAG 1 cut(s) 392
BpuEI CTTGAG 1 cut(s) 854
Bsa29I ATCGAT 2 cut(s) 111, 210
BsaAI YACGTR 1 cut(s) 710
BsaHI GRCGYC 5 cut(s) 17, 56, 75, 192, 258
BsaI GGTCTC 1 cut(s) 696
BsaJI CCNNGG 2 cut(s) 453, 489
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse21I CCTNAGG 1 cut(s) 696
Bse3DI GCAATG 1 cut(s) 602
BseCI ATCGAT 2 cut(s) 111, 210
BseDI CCNNGG 2 cut(s) 453, 489
BseGI GGATG 2 cut(s) 598, 622
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMI GCAATG 1 cut(s) 602
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 4 cut(s) 213, 255, 258, 327
BseYI CCCAGC 1 cut(s) 309
BsgI GTGCAG 1 cut(s) 669
Bsh1236I CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
BshFI GGCC 1 cut(s) 140
BshNI GGYRCC 2 cut(s) 55, 400
BshVI ATCGAT 2 cut(s) 111, 210
BsiHKCI CYCGRG 2 cut(s) 117, 275
BsiSI CCGG 3 cut(s) 59, 78, 183
BslFI GGGAC 1 cut(s) 880
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 3 cut(s) 54, 75, 696
BsmBI CGTCTC 2 cut(s) 54, 75
BsmFI GGGAC 1 cut(s) 880
BsnI GGCC 1 cut(s) 140
Bso31I GGTCTC 1 cut(s) 696
BsoBI CYCGRG 2 cut(s) 117, 275
Bsp143I GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 986
BspACI CCGC 8 cut(s) 25, 86, 202, 237, 282, 381, 519, 548
BspANI GGCC 1 cut(s) 140
BspDI ATCGAT 2 cut(s) 111, 210
BspFNI CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
BspLI GGNNCC 3 cut(s) 57, 402, 868
BspPI GGATC 4 cut(s) 107, 170, 443, 981
BspT107I GGYRCC 2 cut(s) 55, 400
BspTNI GGTCTC 1 cut(s) 696
BsrDI GCAATG 1 cut(s) 602
BssECI CCNNGG 2 cut(s) 453, 489
BssMI GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 986
BssNI GRCGYC 5 cut(s) 17, 56, 75, 192, 258
BssT1I CCWWGG 2 cut(s) 453, 489
Bst4CI ACNGT 3 cut(s) 476, 744, 910
BstACI GRCGYC 5 cut(s) 17, 56, 75, 192, 258
BstBAI YACGTR 1 cut(s) 710
BstC8I GCNNGC 2 cut(s) 41, 546
BstDEI CTNAG 1 cut(s) 696
BstF5I GGATG 2 cut(s) 598, 622
BstFNI CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
BstH2I RGCGCY 2 cut(s) 59, 227
BstHHI GCGC 7 cut(s) 58, 226, 282, 381, 386, 411, 519
BstKTI GATC 8 cut(s) 111, 115, 165, 307, 363, 451, 832, 989
BstMAI GTCTC 3 cut(s) 54, 75, 696
BstMBI GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 986
BstMWI GCNNNNNNNGC 5 cut(s) 83, 146, 277, 315, 604
BstNSI RCATGY 1 cut(s) 233
BstUI CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
BstV1I GCAGC 4 cut(s) 213, 255, 258, 327
BstX2I RGATCY 1 cut(s) 986
BstYI RGATCY 1 cut(s) 986
Bsu15I ATCGAT 2 cut(s) 111, 210
Bsu36I CCTNAGG 1 cut(s) 696
BsuRI GGCC 1 cut(s) 140
BsuTUI ATCGAT 2 cut(s) 111, 210
BtsCI GGATG 2 cut(s) 598, 622
BtsIMutI CAGTG 1 cut(s) 481
Cac8I GCNNGC 2 cut(s) 41, 546
CfoI GCGC 7 cut(s) 58, 226, 282, 381, 386, 411, 519
Cfr13I GGNCC 1 cut(s) 867
ClaI ATCGAT 2 cut(s) 111, 210
CseI GACGC 3 cut(s) 25, 83, 92
Csp6I GTAC 1 cut(s) 133
CviAII CATG 4 cut(s) 230, 353, 797, 948
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 1 cut(s) 696
DinI GGCGCC 1 cut(s) 57
DpnI GATC 8 cut(s) 110, 114, 164, 306, 362, 450, 831, 988
DpnII GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 986
DraI TTTAAA 1 cut(s) 720
EaeI YGGCCR 1 cut(s) 138
Eco130I CCWWGG 2 cut(s) 453, 489
Eco31I GGTCTC 1 cut(s) 696
Eco47I GGWCC 1 cut(s) 867
Eco81I CCTNAGG 1 cut(s) 696
Eco88I CYCGRG 2 cut(s) 117, 275
EcoT14I CCWWGG 2 cut(s) 453, 489
EgeI GGCGCC 1 cut(s) 57
EheI GGCGCC 1 cut(s) 57
ErhI CCWWGG 2 cut(s) 453, 489
Esp3I CGTCTC 2 cut(s) 54, 75
FaeI CATG 4 cut(s) 233, 356, 800, 951
FaqI GGGAC 1 cut(s) 880
FatI CATG 4 cut(s) 229, 352, 796, 947
FbaI TGATCA 1 cut(s) 360
FblI GTMKAC 1 cut(s) 746
Fnu4HI GCNGC 6 cut(s) 227, 237, 269, 272, 316, 382
FokI GGATG 2 cut(s) 605, 609
Fsp4HI GCNGC 6 cut(s) 227, 237, 269, 272, 316, 382
GlaI GCGC 7 cut(s) 57, 225, 281, 380, 385, 410, 518
GluI GCNGC 6 cut(s) 227, 237, 269, 272, 316, 382
GsaI CCCAGC 1 cut(s) 313
GsuI CTGGAG 1 cut(s) 392
HaeII RGCGCY 2 cut(s) 59, 227
HaeIII GGCC 1 cut(s) 140
HapII CCGG 3 cut(s) 59, 78, 183
HgaI GACGC 3 cut(s) 25, 83, 92
HhaI GCGC 7 cut(s) 58, 226, 282, 381, 386, 411, 519
Hin1I GRCGYC 5 cut(s) 17, 56, 75, 192, 258
Hin1II CATG 4 cut(s) 233, 356, 800, 951
Hin6I GCGC 7 cut(s) 56, 224, 280, 379, 384, 409, 517
HinP1I GCGC 7 cut(s) 56, 224, 280, 379, 384, 409, 517
HincII GTYRAC 2 cut(s) 565, 747
HindII GTYRAC 2 cut(s) 565, 747
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 3 cut(s) 68, 179, 860
HpaII CCGG 3 cut(s) 59, 78, 183
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 3 cut(s) 30, 565, 747
Hpy188I TCNGA 4 cut(s) 73, 219, 256, 396
Hpy188III TCNNGA 6 cut(s) 176, 371, 434, 827, 833, 938
Hpy8I GTNNAC 3 cut(s) 30, 565, 747
Hpy99I CGWCG 6 cut(s) 19, 77, 194, 260, 263, 748
HpyAV CCTTC 3 cut(s) 574, 849, 971
HpyCH4III ACNGT 3 cut(s) 476, 744, 910
HpyCH4IV ACGT 3 cut(s) 192, 258, 709
HpyCH4V TGCA 9 cut(s) 229, 329, 422, 637, 650, 713, 764, 776, 796
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 146, 277, 315, 604
HpyF3I CTNAG 1 cut(s) 696
HpySE526I ACGT 3 cut(s) 192, 258, 709
Hsp92I GRCGYC 5 cut(s) 17, 56, 75, 192, 258
Hsp92II CATG 4 cut(s) 233, 356, 800, 951
HspAI GCGC 7 cut(s) 56, 224, 280, 379, 384, 409, 517
KasI GGCGCC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 986
LmnI GCTCC 6 cut(s) 61, 169, 284, 323, 457, 731
Lsp1109I GCAGC 4 cut(s) 213, 255, 258, 327
LweI GCATC 5 cut(s) 195, 322, 518, 631, 931
MaeII ACGT 3 cut(s) 192, 258, 709
MaeIII GTNAC 2 cut(s) 652, 679
MalI GATC 8 cut(s) 110, 114, 164, 306, 362, 450, 831, 988
MboI GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 986
MboII GAAGA 1 cut(s) 929
MflI RGATCY 1 cut(s) 986
MlsI TGGCCA 1 cut(s) 140
MluCI AATT 6 cut(s) 196, 444, 558, 638, 787, 816
MluNI TGGCCA 1 cut(s) 140
Mly113I GGCGCC 1 cut(s) 56
MmeI TCCRAC 2 cut(s) 96, 279
MnlI CCTC 9 cut(s) 82, 85, 91, 97, 117, 333, 402, 544, 562
Mox20I TGGCCA 1 cut(s) 140
MscI TGGCCA 1 cut(s) 140
MseI TTAA 4 cut(s) 294, 414, 719, 912
MslI CAYNNNNRTG 2 cut(s) 627, 952
Msp20I TGGCCA 1 cut(s) 140
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 3 cut(s) 59, 78, 183
MteI GCGCNGCGC 1 cut(s) 382
MvnI CGCG 8 cut(s) 86, 204, 239, 282, 381, 386, 411, 519
MwoI GCNNNNNNNGC 5 cut(s) 83, 146, 277, 315, 604
NarI GGCGCC 1 cut(s) 56
NdeII GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 986
NlaIII CATG 4 cut(s) 233, 356, 800, 951
NlaIV GGNNCC 3 cut(s) 57, 402, 868
NspI RCATGY 1 cut(s) 233
PaeR7I CTCGAG 1 cut(s) 275
PfeI GAWTC 3 cut(s) 68, 179, 860
PkrI GCNGC 6 cut(s) 228, 238, 270, 273, 317, 383
PluTI GGCGCC 1 cut(s) 59
Ppu21I YACGTR 1 cut(s) 710
PspFI CCCAGC 1 cut(s) 309
PspN4I GGNNCC 3 cut(s) 57, 402, 868
PspPI GGNCC 1 cut(s) 867
PspXI VCTCGAGB 1 cut(s) 275
PsuI RGATCY 1 cut(s) 986
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
RseI CAYNNNNRTG 2 cut(s) 627, 952
SalI GTCGAC 1 cut(s) 745
SaqAI TTAA 4 cut(s) 294, 414, 719, 912
SatI GCNGC 6 cut(s) 227, 237, 269, 272, 316, 382
Sau3AI GATC 8 cut(s) 108, 112, 162, 304, 360, 448, 829, 986
Sau96I GGNCC 1 cut(s) 867
SfaNI GCATC 5 cut(s) 195, 322, 518, 631, 931
SfoI GGCGCC 1 cut(s) 57
Sfr274I CTCGAG 1 cut(s) 275
SinI GGWCC 1 cut(s) 867
SlaI CTCGAG 1 cut(s) 275
SmiMI CAYNNNNRTG 2 cut(s) 627, 952
SmlI CTYRAG 2 cut(s) 275, 833
SmoI CTYRAG 2 cut(s) 275, 833
Sse9I AATT 6 cut(s) 196, 444, 558, 638, 787, 816
SsiI CCGC 8 cut(s) 25, 86, 202, 237, 282, 381, 519, 548
SspDI GGCGCC 1 cut(s) 55
StyI CCWWGG 2 cut(s) 453, 489
TaaI ACNGT 3 cut(s) 476, 744, 910
TaiI ACGT 3 cut(s) 195, 261, 712
TaqI TCGA 6 cut(s) 111, 177, 189, 210, 276, 746
TasI AATT 6 cut(s) 196, 444, 558, 638, 787, 816
TatI WGTACW 1 cut(s) 132
TauI GCSGC 2 cut(s) 239, 384
TfiI GAWTC 3 cut(s) 68, 179, 860
Tru1I TTAA 4 cut(s) 294, 414, 719, 912
Tru9I TTAA 4 cut(s) 294, 414, 719, 912
TscAI CASTG 1 cut(s) 481
TseI GCWGC 4 cut(s) 226, 268, 271, 315
TspDTI ATGAA 5 cut(s) 159, 456, 800, 812, 936
TspGWI ACGGA 1 cut(s) 605
TspRI CASTG 1 cut(s) 481
VpaK11BI GGWCC 1 cut(s) 867
XceI RCATGY 1 cut(s) 233
XhoI CTCGAG 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 746
ZraI GACGTC 2 cut(s) 193, 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.