Rh5CG311000

Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0031230
Physical Location & Seq
Forward (+)
34310916 .. 34312098
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG311000.1

Sequence Viewer

Length: 345 bp
ATGTATTTTGCTGATATATTTGGTTTTGCAGCTAAATCCGGTGTCAATCCTTCTGGTTGTGTTGAACTTGCAAAACATGTCAAATTGCAGTGCCCAAATCTAGTGCTTTCTGGCTTAATGACAATAGGGATGCGTGACTATACATCAACTCCAGAGAACTTCAGGATGCTATCTAAGTGTAGAACTGAGGTATGCAAGGAGCTTGGTATGGCAGAGGAGAACTTTGAGCTGTCAATGGGCATGTCTGGTGATTTTGAGCAAGCGATTGAAATGGGCAGCACCAATGTCAGAGTTGGATCCACCATATTTGGGCCAAGGGAATATCCAAAGAAACAACCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ala_racemase_N

Protein Analysis

114

Amino Acids

12.6

Weight (kDa)

5.81

Isoelectric Point (pI)

28.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ala_racemase_N PF01168 12 - 105 7.5e-06 Alanine racemase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0031230)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002848.1_g000021
rosa_samantha Rh5CG311000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 291, 304
AcuI CTGAAG 1 cut(s) 145
AfiI CCNNNNNNNGG 1 cut(s) 309
AflIII ACRYGT 1 cut(s) 76
AgsI TTSAA 2 cut(s) 65, 269
AluBI AGCT 3 cut(s) 32, 202, 229
AluI AGCT 3 cut(s) 32, 202, 229
AlwI GGATC 2 cut(s) 291, 304
AoxI GGCC 1 cut(s) 311
ApeKI GCWGC 2 cut(s) 29, 276
AspS9I GGNCC 1 cut(s) 311
AsuHPI GGTGA 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 95
BamHI GGATCC 1 cut(s) 296
BbvI GCAGC 2 cut(s) 41, 288
BfaI CTAG 1 cut(s) 101
BisI GCNGC 2 cut(s) 30, 277
BlsI GCNGC 2 cut(s) 31, 278
BmgT120I GGNCC 1 cut(s) 311
BmiI GGNNCC 1 cut(s) 298
BmsI GCATC 2 cut(s) 120, 156
BpmI CTGGAG 1 cut(s) 135
BsaJI CCNNGG 1 cut(s) 314
BsaWI WCCGGW 1 cut(s) 38
BsaXI ACNNNNNCTCC 2 cut(s) 133, 163
Bsc4I CCNNNNNNNGG 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 314
BseGI GGATG 2 cut(s) 135, 171
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMII CTCAG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 230
BseSI GKGCMC 1 cut(s) 95
BseXI GCAGC 2 cut(s) 41, 288
BshFI GGCC 1 cut(s) 313
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 1 cut(s) 309
BsnI GGCC 1 cut(s) 313
Bsp1286I GDGCHC 1 cut(s) 95
Bsp143I GATC 1 cut(s) 296
BspANI GGCC 1 cut(s) 313
BspCNI CTCAG 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 298
BspPI GGATC 2 cut(s) 291, 304
BssECI CCNNGG 1 cut(s) 314
BssMI GATC 1 cut(s) 296
BssT1I CCWWGG 1 cut(s) 314
BstC8I GCNNGC 1 cut(s) 261
BstDEI CTNAG 2 cut(s) 174, 186
BstF5I GGATG 2 cut(s) 135, 171
BstKTI GATC 1 cut(s) 299
BstMBI GATC 1 cut(s) 296
BstNSI RCATGY 2 cut(s) 80, 244
BstSLI GKGCMC 1 cut(s) 95
BstV1I GCAGC 2 cut(s) 41, 288
BstX2I RGATCY 1 cut(s) 296
BstYI RGATCY 1 cut(s) 296
BsuRI GGCC 1 cut(s) 313
BtsCI GGATG 2 cut(s) 135, 171
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 95
Cac8I GCNNGC 1 cut(s) 261
Cfr13I GGNCC 1 cut(s) 311
CspCI CAANNNNNGTGG 2 cut(s) 289, 324
CviAII CATG 2 cut(s) 77, 241
CviJI RGCY 5 cut(s) 32, 114, 202, 229, 313
CviKI_1 RGCY 5 cut(s) 32, 114, 202, 229, 313
DdeI CTNAG 2 cut(s) 174, 186
DpnI GATC 1 cut(s) 298
DpnII GATC 1 cut(s) 296
Eco130I CCWWGG 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 145
EcoT14I CCWWGG 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 314
FaeI CATG 2 cut(s) 80, 244
FaiI YATR 7 cut(s) 17, 78, 141, 193, 209, 242, 305
FatI CATG 2 cut(s) 76, 240
Fnu4HI GCNGC 2 cut(s) 30, 277
FokI GGATG 2 cut(s) 142, 178
Fsp4HI GCNGC 2 cut(s) 30, 277
FspBI CTAG 1 cut(s) 101
GluI GCNGC 2 cut(s) 30, 277
GsuI CTGGAG 1 cut(s) 135
HaeIII GGCC 1 cut(s) 313
HapII CCGG 1 cut(s) 39
Hin1II CATG 2 cut(s) 80, 244
HpaII CCGG 1 cut(s) 39
HphI GGTGA 1 cut(s) 260
Hpy188I TCNGA 1 cut(s) 290
Hpy188III TCNNGA 2 cut(s) 152, 163
HpyAV CCTTC 1 cut(s) 60
HpyCH4V TGCA 4 cut(s) 29, 71, 88, 195
HpyF3I CTNAG 2 cut(s) 174, 186
Hsp92II CATG 2 cut(s) 80, 244
Kzo9I GATC 1 cut(s) 296
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 6 cut(s) 39, 52, 96, 148, 165, 231
Lsp1109I GCAGC 2 cut(s) 41, 288
LweI GCATC 2 cut(s) 120, 156
MaeI CTAG 1 cut(s) 101
MaeIII GTNAC 1 cut(s) 134
MalI GATC 1 cut(s) 298
MboI GATC 1 cut(s) 296
MflI RGATCY 1 cut(s) 296
MhlI GDGCHC 1 cut(s) 95
MluCI AATT 2 cut(s) 83, 340
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 2 cut(s) 181, 208
MseI TTAA 1 cut(s) 116
MspI CCGG 1 cut(s) 39
NdeII GATC 1 cut(s) 296
NlaIII CATG 2 cut(s) 80, 244
NlaIV GGNNCC 1 cut(s) 298
NmuCI GTSAC 1 cut(s) 134
NspI RCATGY 2 cut(s) 80, 244
PciI ACATGT 1 cut(s) 76
PkrI GCNGC 2 cut(s) 31, 278
PscI ACATGT 1 cut(s) 76
PspN4I GGNNCC 1 cut(s) 298
PspPI GGNCC 1 cut(s) 311
PsuI RGATCY 1 cut(s) 296
SaqAI TTAA 1 cut(s) 116
SatI GCNGC 2 cut(s) 30, 277
Sau3AI GATC 1 cut(s) 296
Sau96I GGNCC 1 cut(s) 311
SduI GDGCHC 1 cut(s) 95
SetI ASST 4 cut(s) 34, 192, 204, 231
SfaNI GCATC 2 cut(s) 120, 156
Sse9I AATT 2 cut(s) 83, 340
SspMI CTAG 1 cut(s) 101
StyI CCWWGG 1 cut(s) 314
TasI AATT 2 cut(s) 83, 340
Tru1I TTAA 1 cut(s) 116
Tru9I TTAA 1 cut(s) 116
TscAI CASTG 1 cut(s) 95
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 2 cut(s) 29, 276
Tsp45I GTSAC 1 cut(s) 134
TspRI CASTG 1 cut(s) 95
XceI RCATGY 2 cut(s) 80, 244
XspI CTAG 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.