Rh5CG391600

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0025042
Physical Location & Seq
Reverse (-)
51552737 .. 51553132
396 bp
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UTR
Exon/CDS
Intron
Rh5CG391600.1

Sequence Viewer

Length: 396 bp
ATGGAGAACCAGGCCAAAAATGGGACCTTTAATATGCCAGTTGGACGTGGAAAAACCTATCTTCTTCAGATCATTAGTGCAGTAATGGATGAAGAGCTATTCTTCGGCATTGCACATCACAAGCTGATATTAGTCGGAAAAGATGGTTCCTATACAAAACAAGTTGGAACCTCCTACATAATGATAGCCCCTGGCCAATCCATGGACCTGTTATTAGAAGCAAACCAACCTAATGGGCTCTATGTCATGGCTGCAAGAGCATATTCAAGCGCCATCGGAGCTGGTTTTGACAAGACCATGACATCTGCCATTATTAAATATCATGGTTCAGATGATCACGATTCACGACCACCACCCTCGAGGACGTCCTGCTTTCCCACATCTTTCACCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

131

Amino Acids

14.25

Weight (kDa)

6.9

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase PF00394 6 - 110 6.8e-25 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025042)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0054421
rosa_rugosa Rorug05G0286100
rosa_samantha Rh5CG391600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 368
AbsI CCTCGAGG 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 202
AcoI YGGCCR 1 cut(s) 193
AcuI CTGAAG 1 cut(s) 50
AcyI GRCGYC 1 cut(s) 365
AfiI CCNNNNNNNGG 2 cut(s) 21, 202
AgsI TTSAA 1 cut(s) 267
AjiI CACGTC 1 cut(s) 47
AjnI CCWGG 2 cut(s) 9, 190
AluBI AGCT 3 cut(s) 97, 124, 281
AluI AGCT 3 cut(s) 97, 124, 281
Ama87I CYCGRG 1 cut(s) 358
AoxI GGCC 2 cut(s) 12, 193
ApeKI GCWGC 1 cut(s) 251
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 2 cut(s) 24, 205
AsuHPI GGTGA 1 cut(s) 379
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 2 cut(s) 24, 205
BalI TGGCCA 1 cut(s) 195
BanII GRGCYC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 238
BccI CCATC 2 cut(s) 137, 281
BciT130I CCWGG 2 cut(s) 11, 192
BclI TGATCA 1 cut(s) 334
BfoI RGCGCY 1 cut(s) 273
BisI GCNGC 1 cut(s) 252
BlsI GCNGC 1 cut(s) 253
Bme1390I CCNGG 2 cut(s) 11, 192
Bme18I GGWCC 2 cut(s) 24, 205
BmeT110I CYCGRG 1 cut(s) 358
BmgBI CACGTC 1 cut(s) 47
BmgT120I GGNCC 2 cut(s) 24, 205
BmiI GGNNCC 3 cut(s) 25, 148, 169
BmrFI CCNGG 2 cut(s) 11, 192
BsaHI GRCGYC 1 cut(s) 365
BsaJI CCNNGG 2 cut(s) 190, 201
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 202
Bse1I ACTGG 1 cut(s) 38
Bse3DI GCAATG 1 cut(s) 108
BseBI CCWGG 2 cut(s) 11, 192
BseDI CCNNGG 2 cut(s) 190, 201
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 2 cut(s) 21, 202
BseMI GCAATG 1 cut(s) 108
BseNI ACTGG 1 cut(s) 38
BseXI GCAGC 1 cut(s) 238
BsgI GTGCAG 1 cut(s) 99
BshFI GGCC 2 cut(s) 14, 195
BsiHKCI CYCGRG 1 cut(s) 358
BslFI GGGAC 1 cut(s) 37
BslI CCNNNNNNNGG 2 cut(s) 21, 202
BsmFI GGGAC 1 cut(s) 37
BsnI GGCC 2 cut(s) 14, 195
BsoBI CYCGRG 1 cut(s) 358
Bsp1286I GDGCHC 1 cut(s) 240
Bsp143I GATC 2 cut(s) 69, 334
Bsp19I CCATGG 1 cut(s) 201
BspANI GGCC 2 cut(s) 14, 195
BspLI GGNNCC 3 cut(s) 25, 148, 169
BspQI GCTCTTC 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 108
BsrI ACTGG 1 cut(s) 38
BssECI CCNNGG 2 cut(s) 190, 201
BssMI GATC 2 cut(s) 69, 334
BssNI GRCGYC 1 cut(s) 365
BssT1I CCWWGG 1 cut(s) 201
Bst2UI CCWGG 2 cut(s) 11, 192
Bst6I CTCTTC 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 365
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 94
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 2 cut(s) 72, 337
BstMBI GATC 2 cut(s) 69, 334
BstMWI GCNNNNNNNGC 2 cut(s) 257, 278
BstNI CCWGG 2 cut(s) 11, 192
BstSCI CCNGG 2 cut(s) 9, 190
BstV1I GCAGC 1 cut(s) 238
BstXI CCANNNNNNTGG 1 cut(s) 233
BsuRI GGCC 2 cut(s) 14, 195
BtgI CCRYGG 1 cut(s) 201
BtrI CACGTC 1 cut(s) 47
BtsCI GGATG 1 cut(s) 94
CfoI GCGC 1 cut(s) 272
Cfr13I GGNCC 2 cut(s) 24, 205
CviAII CATG 4 cut(s) 202, 247, 298, 323
CviJI RGCY 8 cut(s) 14, 97, 124, 188, 195, 238, 251, 281
CviKI_1 RGCY 8 cut(s) 14, 97, 124, 188, 195, 238, 251, 281
DpnI GATC 2 cut(s) 71, 336
DpnII GATC 2 cut(s) 69, 334
EaeI YGGCCR 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 240
Eco47I GGWCC 2 cut(s) 24, 205
Eco57I CTGAAG 1 cut(s) 50
Eco88I CYCGRG 1 cut(s) 358
EcoO109I RGGNCCY 1 cut(s) 24
EcoRII CCWGG 2 cut(s) 9, 190
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 4 cut(s) 205, 250, 301, 326
FaqI GGGAC 1 cut(s) 37
FatI CATG 4 cut(s) 201, 246, 297, 322
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 1 cut(s) 252
FokI GGATG 1 cut(s) 101
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 252
GlaI GCGC 1 cut(s) 271
GluI GCNGC 1 cut(s) 252
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 2 cut(s) 14, 195
HhaI GCGC 1 cut(s) 272
Hin1I GRCGYC 1 cut(s) 365
Hin1II CATG 4 cut(s) 205, 250, 301, 326
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HinfI GANTC 1 cut(s) 341
HphI GGTGA 1 cut(s) 379
Hpy188I TCNGA 4 cut(s) 69, 137, 278, 331
Hpy188III TCNNGA 2 cut(s) 338, 345
HpyCH4IV ACGT 2 cut(s) 46, 365
HpyCH4V TGCA 3 cut(s) 80, 113, 254
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 278
HpySE526I ACGT 2 cut(s) 46, 365
Hsp92I GRCGYC 1 cut(s) 365
Hsp92II CATG 4 cut(s) 205, 250, 301, 326
HspAI GCGC 1 cut(s) 270
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 69, 334
LguI GCTCTTC 1 cut(s) 87
LmnI GCTCC 1 cut(s) 278
LpnPI CCDG 7 cut(s) 23, 51, 177, 204, 221, 267, 382
Lsp1109I GCAGC 1 cut(s) 238
MaeII ACGT 2 cut(s) 46, 365
MalI GATC 2 cut(s) 71, 336
MboI GATC 2 cut(s) 69, 334
MboII GAAGA 4 cut(s) 53, 56, 94, 104
MhlI GDGCHC 1 cut(s) 240
MlsI TGGCCA 1 cut(s) 195
MluNI TGGCCA 1 cut(s) 195
MmeI TCCRAC 3 cut(s) 22, 115, 145
MnlI CCTC 3 cut(s) 181, 354, 367
Mox20I TGGCCA 1 cut(s) 195
MscI TGGCCA 1 cut(s) 195
MseI TTAA 2 cut(s) 30, 315
Msp20I TGGCCA 1 cut(s) 195
MspR9I CCNGG 2 cut(s) 11, 192
MvaI CCWGG 2 cut(s) 11, 192
MwoI GCNNNNNNNGC 2 cut(s) 257, 278
NcoI CCATGG 1 cut(s) 201
NdeII GATC 2 cut(s) 69, 334
NlaIII CATG 4 cut(s) 205, 250, 301, 326
NlaIV GGNNCC 3 cut(s) 25, 148, 169
PaeR7I CTCGAG 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 87
PfeI GAWTC 1 cut(s) 341
PflMI CCANNNNNTGG 1 cut(s) 202
PkrI GCNGC 1 cut(s) 253
PpuMI RGGWCCY 1 cut(s) 24
Psp5II RGGWCCY 1 cut(s) 24
Psp6I CCWGG 2 cut(s) 9, 190
PspGI CCWGG 2 cut(s) 9, 190
PspN4I GGNNCC 3 cut(s) 25, 148, 169
PspPI GGNCC 2 cut(s) 24, 205
PspPPI RGGWCCY 1 cut(s) 24
PspXI VCTCGAGB 1 cut(s) 358
SapI GCTCTTC 1 cut(s) 87
SaqAI TTAA 2 cut(s) 30, 315
SatI GCNGC 1 cut(s) 252
Sau3AI GATC 2 cut(s) 69, 334
Sau96I GGNCC 2 cut(s) 24, 205
ScrFI CCNGG 2 cut(s) 11, 192
SduI GDGCHC 1 cut(s) 240
Sfr274I CTCGAG 1 cut(s) 358
SinI GGWCC 2 cut(s) 24, 205
SlaI CTCGAG 1 cut(s) 358
SmlI CTYRAG 1 cut(s) 358
SmoI CTYRAG 1 cut(s) 358
StyD4I CCNGG 2 cut(s) 9, 190
StyI CCWWGG 1 cut(s) 201
TaiI ACGT 2 cut(s) 49, 368
TaqI TCGA 1 cut(s) 359
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 2 cut(s) 30, 315
Tru9I TTAA 2 cut(s) 30, 315
TseI GCWGC 1 cut(s) 251
TspDTI ATGAA 1 cut(s) 105
Van91I CCANNNNNTGG 1 cut(s) 202
VpaK11BI GGWCC 2 cut(s) 24, 205
XcmI CCANNNNNNNNNTGG 1 cut(s) 17
XhoI CTCGAG 1 cut(s) 358
ZraI GACGTC 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.