Rh5DG375500

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0033506
Physical Location & Seq
Reverse (-)
57053638 .. 57054468
831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG375500.1

Sequence Viewer

Length: 831 bp
ATGGCGGATTTTATAGGGGCTGTTTTAGTGGGTCTTGCTTTAGATGGTCTACTAGATAAAGCAACTGAATTGTTCAGTAAAAATATAAAACTGTGCATCAGCTCTCTGGAGTTTAGTAACTTCGTACGGGATTTCGCATATGCAGTAGATTATTTAGAATCTCAGATCGAAAAGGTAGACGATCAAAACAAGGAATTAAAAAAGACGATACAGAGCGCCAGAGGGCTCCTTCACAAGTGTCGGAGACTTAGTAATAGTAGGTGGAACGCCATCAAAAGATATAGATACACCGCGAAGGTCTTTCGATTAAATAAAGCTTTTAAAATTCATGTAAAGATCTTGGAGTTGATGAATCAGATGGAGATCTTGGAATTGTCAAGGAAATACCGCGACCAATTTGGTGAATCAGCAAACGTTGAGAACGAGATCTTACAATTGGAGAGGTATATAGCGGTGATGACCGAGCAAATTGGTAGTACTACCATGCGAAGGGTAGCTTTTAAGGATGTTCCTTTGAATAATCTAAATGATCTGGTACGTAGTCAAATTAAAATTGCAAGTTTTAAAGCTGGAATGGGTGTTCCTTTGAAGGCGAATCTAATGGAAGCTGTAAATGATACTGAAATTAAAGCATCATCCTTTCACGTTGAACTGGATGTGCCTATAAGGGAACTGAAGAAGAAGCTCTTTACGGATGGGGTGTCAATGCTTGTTGTAAATGGTAATGGAGGATGCGGGAAAACCAAAGTGGCAAAGCAGTTTTGTCAAGATGACGAAGTCAAAGGTGCGTGCGTGCGTGTGTGTTTTTTATTTTTTAGAATGTGTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

276

Amino Acids

31.41

Weight (kDa)

9.22

Isoelectric Point (pI)

31.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 42 - 128 5.4e-09 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0033506)

Species Orthologous Gene IDs
rosa_samantha Rh5BG360700 Rh5DG375500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 49, 177
AccII CGCG 2 cut(s) 293, 390
AciI CCGC 5 cut(s) 5, 291, 388, 452, 735
AclI AACGTT 1 cut(s) 414
AcsI RAATTY 1 cut(s) 324
AcuI CTGAAG 1 cut(s) 695
AfaI GTAC 3 cut(s) 126, 478, 537
AfiI CCNNNNNNNGG 1 cut(s) 489
AgsI TTSAA 3 cut(s) 517, 589, 650
AloI GAACNNNNNNTCC 2 cut(s) 564, 596
AluBI AGCT 6 cut(s) 102, 317, 497, 569, 608, 685
AluI AGCT 6 cut(s) 102, 317, 497, 569, 608, 685
Alw26I GTCTC 1 cut(s) 238
ApoI RAATTY 1 cut(s) 324
AspLEI GCGC 1 cut(s) 218
AsuHPI GGTGA 2 cut(s) 413, 466
BanII GRGCYC 1 cut(s) 228
BccI CCATC 4 cut(s) 38, 278, 352, 689
BcoDI GTCTC 1 cut(s) 238
BfaI CTAG 1 cut(s) 53
BfoI RGCGCY 1 cut(s) 219
BglII AGATCT 3 cut(s) 336, 363, 426
BmcAI AGTACT 1 cut(s) 478
BmiI GGNNCC 1 cut(s) 227
BmsI GCATC 3 cut(s) 105, 641, 722
BpmI CTGGAG 1 cut(s) 128
BsaAI YACGTR 1 cut(s) 539
BsaBI GATNNNNATC 1 cut(s) 362
BsaXI ACNNNNNCTCC 2 cut(s) 235, 265
Bsc4I CCNNNNNNNGG 1 cut(s) 489
Bse1I ACTGG 1 cut(s) 657
Bse8I GATNNNNATC 1 cut(s) 362
BseGI GGATG 5 cut(s) 511, 635, 661, 700, 737
BseJI GATNNNNATC 1 cut(s) 362
BseLI CCNNNNNNNGG 1 cut(s) 489
BseMII CTCAG 1 cut(s) 176
BseNI ACTGG 1 cut(s) 657
Bsh1236I CGCG 2 cut(s) 293, 390
BsiWI CGTACG 1 cut(s) 124
BslI CCNNNNNNNGG 1 cut(s) 489
BsmAI GTCTC 1 cut(s) 238
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 6 cut(s) 165, 181, 336, 363, 426, 529
BspACI CCGC 5 cut(s) 5, 291, 388, 452, 735
BspCNI CTCAG 1 cut(s) 175
BspFNI CGCG 2 cut(s) 293, 390
BspLI GGNNCC 1 cut(s) 227
BsrI ACTGG 1 cut(s) 657
BssMI GATC 6 cut(s) 165, 181, 336, 363, 426, 529
Bst4CI ACNGT 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 539
BstC8I GCNNGC 2 cut(s) 790, 794
BstDEI CTNAG 2 cut(s) 162, 248
BstF5I GGATG 5 cut(s) 511, 635, 661, 700, 737
BstFNI CGCG 2 cut(s) 293, 390
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 6 cut(s) 168, 184, 339, 366, 429, 532
BstMAI GTCTC 1 cut(s) 238
BstMBI GATC 6 cut(s) 165, 181, 336, 363, 426, 529
BstSNI TACGTA 1 cut(s) 539
BstUI CGCG 2 cut(s) 293, 390
BstX2I RGATCY 3 cut(s) 336, 363, 426
BstYI RGATCY 3 cut(s) 336, 363, 426
BtsCI GGATG 5 cut(s) 511, 635, 661, 700, 737
Cac8I GCNNGC 2 cut(s) 790, 794
CfoI GCGC 1 cut(s) 218
Csp6I GTAC 3 cut(s) 125, 477, 536
CviAII CATG 2 cut(s) 329, 484
CviJI RGCY 8 cut(s) 20, 102, 226, 317, 497, 569, 608, 685
CviKI_1 RGCY 8 cut(s) 20, 102, 226, 317, 497, 569, 608, 685
CviQI GTAC 3 cut(s) 125, 477, 536
DdeI CTNAG 2 cut(s) 162, 248
DpnI GATC 6 cut(s) 167, 183, 338, 365, 428, 531
DpnII GATC 6 cut(s) 165, 181, 336, 363, 426, 529
DraI TTTAAA 2 cut(s) 322, 565
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 539
Eco24I GRGCYC 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 695
EcoT38I GRGCYC 1 cut(s) 228
FaeI CATG 2 cut(s) 332, 487
FalI AAGNNNNNCTT 4 cut(s) 481, 513, 671, 703
FatI CATG 2 cut(s) 328, 483
FauI CCCGC 1 cut(s) 728
FauNDI CATATG 1 cut(s) 139
FblI GTMKAC 2 cut(s) 49, 177
FokI GGATG 5 cut(s) 518, 622, 668, 707, 744
FriOI GRGCYC 1 cut(s) 228
FspBI CTAG 1 cut(s) 53
GlaI GCGC 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 128
HaeII RGCGCY 1 cut(s) 219
HhaI GCGC 1 cut(s) 218
Hin1II CATG 2 cut(s) 332, 487
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 4 cut(s) 158, 352, 404, 595
HphI GGTGA 2 cut(s) 413, 466
Hpy166II GTNNAC 2 cut(s) 50, 178
Hpy188I TCNGA 3 cut(s) 165, 243, 357
Hpy188III TCNNGA 2 cut(s) 107, 767
Hpy8I GTNNAC 2 cut(s) 50, 178
HpyAV CCTTC 4 cut(s) 239, 289, 483, 583
HpyCH4III ACNGT 1 cut(s) 93
HpyCH4IV ACGT 3 cut(s) 414, 538, 645
HpyCH4V TGCA 3 cut(s) 96, 143, 557
HpyF3I CTNAG 2 cut(s) 162, 248
HpySE526I ACGT 3 cut(s) 414, 538, 645
Hsp92II CATG 2 cut(s) 332, 487
HspAI GCGC 1 cut(s) 216
Kzo9I GATC 6 cut(s) 165, 181, 336, 363, 426, 529
LmnI GCTCC 1 cut(s) 231
LpnPI CCDG 5 cut(s) 92, 232, 518, 555, 638
LweI GCATC 3 cut(s) 105, 641, 722
MaeI CTAG 1 cut(s) 53
MaeII ACGT 3 cut(s) 414, 538, 645
MaeIII GTNAC 1 cut(s) 116
MalI GATC 6 cut(s) 167, 183, 338, 365, 428, 531
MboI GATC 6 cut(s) 165, 181, 336, 363, 426, 529
MboII GAAGA 2 cut(s) 688, 691
MfeI CAATTG 1 cut(s) 434
MflI RGATCY 3 cut(s) 336, 363, 426
MhlI GDGCHC 1 cut(s) 228
MmeI TCCRAC 1 cut(s) 221
MnlI CCTC 3 cut(s) 215, 435, 722
MseI TTAA 7 cut(s) 197, 308, 321, 501, 549, 564, 627
MunI CAATTG 1 cut(s) 434
MvnI CGCG 2 cut(s) 293, 390
NdeI CATATG 1 cut(s) 139
NdeII GATC 6 cut(s) 165, 181, 336, 363, 426, 529
NlaIII CATG 2 cut(s) 332, 487
NlaIV GGNNCC 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 420
PfeI GAWTC 4 cut(s) 158, 352, 404, 595
Pfl23II CGTACG 1 cut(s) 124
PflFI GACNNNGTC 1 cut(s) 776
Ppu21I YACGTR 1 cut(s) 539
Psp1406I AACGTT 1 cut(s) 414
PspLI CGTACG 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 227
PsuI RGATCY 3 cut(s) 336, 363, 426
PsyI GACNNNGTC 1 cut(s) 776
RsaI GTAC 3 cut(s) 126, 478, 537
RsaNI GTAC 3 cut(s) 125, 477, 536
SaqAI TTAA 7 cut(s) 197, 308, 321, 501, 549, 564, 627
Sau3AI GATC 6 cut(s) 165, 181, 336, 363, 426, 529
ScaI AGTACT 1 cut(s) 478
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 3 cut(s) 105, 641, 722
SnaBI TACGTA 1 cut(s) 539
SsiI CCGC 5 cut(s) 5, 291, 388, 452, 735
SspMI CTAG 1 cut(s) 53
TaaI ACNGT 1 cut(s) 93
TaiI ACGT 3 cut(s) 417, 541, 648
TaqI TCGA 2 cut(s) 168, 304
TaqII GACCGA 1 cut(s) 476
TatI WGTACW 1 cut(s) 476
TfiI GAWTC 4 cut(s) 158, 352, 404, 595
Tru1I TTAA 7 cut(s) 197, 308, 321, 501, 549, 564, 627
Tru9I TTAA 7 cut(s) 197, 308, 321, 501, 549, 564, 627
TspDTI ATGAA 2 cut(s) 317, 365
TspGWI ACGGA 1 cut(s) 707
Tth111I GACNNNGTC 1 cut(s) 776
XapI RAATTY 1 cut(s) 324
XmiI GTMKAC 2 cut(s) 49, 177
XspI CTAG 1 cut(s) 53
ZrmI AGTACT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.