Rh5DG399800

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0024680
Physical Location & Seq
Reverse (-)
63013916 .. 63014254
339 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG399800.1

Sequence Viewer

Length: 339 bp
ATGTACAGATTCCTGGTGGACGATGTGCCGATAAGAAGGTACGCAAGGAAGAGCGATGCCACATTCCCATTGAGGCCGATGTGGGTGTACGGATCCATATGGGATGCATCATCATGGGCCACAGAGGAAGGAAAATACAAAGCCGACTACAACTACCAGCCATTCATTGGTAGGTACAACAACTTCAAACTAGGCCCAGCCTCCGTTGCCTCGCCCTCCGGCACCTCCGGGCTAAGCCGGCAGCAGTACGCGGCAATGGATTGGGTGCAGAGGAACTACAAGGTATACGACTACTGCACCGACTCCAGGCGAGACCATTCGCAGACACCGGAGTGTTAG

Protein Analysis

112

Amino Acids

13.12

Weight (kDa)

8.88

Isoelectric Point (pI)

56.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 3 - 51 4.9e-12 Glycosyl hydrolases family 16
XET_C PF06955 76 - 112 4.9e-14 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0024680)

Species Orthologous Gene IDs
pyrus_communis pycom11g11980
rosa_multiflora Rmu_sc0009185.1_g000030
rosa_samantha Rh5DG399800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 221
AccB7I CCANNNNNTGG 1 cut(s) 167
AccI GTMKAC 1 cut(s) 285
AccII CGCG 1 cut(s) 251
AciI CCGC 1 cut(s) 251
AclWI GGATC 2 cut(s) 87, 100
AfaI GTAC 5 cut(s) 5, 41, 89, 176, 248
AfiI CCNNNNNNNGG 2 cut(s) 167, 306
AgsI TTSAA 1 cut(s) 187
AjnI CCWGG 2 cut(s) 12, 305
AleI CACNNNNGTG 1 cut(s) 331
Alw26I GTCTC 1 cut(s) 306
AlwI GGATC 2 cut(s) 87, 100
AoxI GGCC 3 cut(s) 74, 117, 193
ApeKI GCWGC 1 cut(s) 241
AspS9I GGNCC 2 cut(s) 117, 194
AsuC2I CCSGG 1 cut(s) 229
BamHI GGATCC 1 cut(s) 92
BanI GGYRCC 1 cut(s) 221
BarI GAAGNNNNNNTAC 2 cut(s) 167, 199
BbvI GCAGC 1 cut(s) 253
BciT130I CCWGG 2 cut(s) 14, 307
BcnI CCSGG 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 306
BfaI CTAG 1 cut(s) 191
BisI GCNGC 2 cut(s) 242, 252
BlpI GCTNAGC 1 cut(s) 233
BlsI GCNGC 2 cut(s) 243, 253
Bme1390I CCNGG 3 cut(s) 14, 229, 307
BmgT120I GGNCC 2 cut(s) 117, 194
BmiI GGNNCC 2 cut(s) 94, 223
BmrFI CCNGG 3 cut(s) 14, 229, 307
BmsI GCATC 3 cut(s) 46, 94, 116
BpmI CTGGAG 1 cut(s) 289
Bpu1102I GCTNAGC 1 cut(s) 233
BpuMI CCSGG 1 cut(s) 229
BsaI GGTCTC 1 cut(s) 306
BsaWI WCCGGW 1 cut(s) 328
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 306
Bse118I RCCGGY 1 cut(s) 237
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 2 cut(s) 14, 307
BseGI GGATG 1 cut(s) 109
BseLI CCNNNNNNNGG 2 cut(s) 167, 306
BseMI GCAATG 1 cut(s) 261
BseXI GCAGC 1 cut(s) 253
BseYI CCCAGC 1 cut(s) 196
BsgI GTGCAG 2 cut(s) 280, 287
Bsh1236I CGCG 1 cut(s) 251
BshFI GGCC 3 cut(s) 76, 119, 195
BshNI GGYRCC 1 cut(s) 221
BsiSI CCGG 4 cut(s) 219, 228, 238, 329
BslI CCNNNNNNNGG 2 cut(s) 167, 306
BsmAI GTCTC 1 cut(s) 306
BsnI GGCC 3 cut(s) 76, 119, 195
Bso31I GGTCTC 1 cut(s) 306
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 92
Bsp1720I GCTNAGC 1 cut(s) 233
BspACI CCGC 1 cut(s) 251
BspANI GGCC 3 cut(s) 76, 119, 195
BspFNI CGCG 1 cut(s) 251
BspLI GGNNCC 2 cut(s) 94, 223
BspPI GGATC 2 cut(s) 87, 100
BspQI GCTCTTC 1 cut(s) 44
BspT107I GGYRCC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 306
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 237
BsrGI TGTACA 1 cut(s) 3
BssAI RCCGGY 1 cut(s) 237
BssMI GATC 1 cut(s) 92
BssNAI GTATAC 1 cut(s) 286
Bst1107I GTATAC 1 cut(s) 286
Bst2UI CCWGG 2 cut(s) 14, 307
Bst6I CTCTTC 1 cut(s) 44
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 1 cut(s) 239
BstDEI CTNAG 1 cut(s) 233
BstF5I GGATG 1 cut(s) 109
BstFNI CGCG 1 cut(s) 251
BstKTI GATC 1 cut(s) 95
BstMAI GTCTC 1 cut(s) 306
BstMBI GATC 1 cut(s) 92
BstMWI GCNNNNNNNGC 2 cut(s) 206, 238
BstNI CCWGG 2 cut(s) 14, 307
BstSCI CCNGG 3 cut(s) 12, 227, 305
BstUI CGCG 1 cut(s) 251
BstV1I GCAGC 1 cut(s) 253
BstX2I RGATCY 1 cut(s) 92
BstYI RGATCY 1 cut(s) 92
BstZ17I GTATAC 1 cut(s) 286
BsuRI GGCC 3 cut(s) 76, 119, 195
BtgZI GCGATG 1 cut(s) 69
BtsCI GGATG 1 cut(s) 109
Cac8I GCNNGC 1 cut(s) 239
Cfr10I RCCGGY 1 cut(s) 237
Cfr13I GGNCC 2 cut(s) 117, 194
Csp6I GTAC 5 cut(s) 4, 40, 88, 175, 247
CviAII CATG 1 cut(s) 114
CviJI RGCY 8 cut(s) 76, 119, 143, 160, 195, 200, 232, 237
CviKI_1 RGCY 8 cut(s) 76, 119, 143, 160, 195, 200, 232, 237
CviQI GTAC 5 cut(s) 4, 40, 88, 175, 247
DdeI CTNAG 1 cut(s) 233
DpnI GATC 1 cut(s) 94
DpnII GATC 1 cut(s) 92
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 306
EcoRII CCWGG 2 cut(s) 12, 305
EcoT22I ATGCAT 1 cut(s) 109
FaeI CATG 1 cut(s) 117
FaiI YATR 4 cut(s) 98, 100, 115, 286
FatI CATG 1 cut(s) 113
FauNDI CATATG 1 cut(s) 98
FblI GTMKAC 1 cut(s) 285
Fnu4HI GCNGC 2 cut(s) 242, 252
FokI GGATG 1 cut(s) 116
Fsp4HI GCNGC 2 cut(s) 242, 252
FspBI CTAG 1 cut(s) 191
GluI GCNGC 2 cut(s) 242, 252
GsaI CCCAGC 1 cut(s) 200
GsuI CTGGAG 1 cut(s) 289
HaeIII GGCC 3 cut(s) 76, 119, 195
HapII CCGG 4 cut(s) 219, 228, 238, 329
Hin1II CATG 1 cut(s) 117
HinfI GANTC 2 cut(s) 9, 302
HpaII CCGG 4 cut(s) 219, 228, 238, 329
Hpy166II GTNNAC 3 cut(s) 19, 88, 286
Hpy8I GTNNAC 3 cut(s) 19, 88, 286
HpyAV CCTTC 2 cut(s) 30, 122
HpyCH4V TGCA 3 cut(s) 107, 268, 297
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 238
HpyF3I CTNAG 1 cut(s) 233
Hsp92II CATG 1 cut(s) 117
KroI GCCGGC 1 cut(s) 237
KroNI GCCGGC 1 cut(s) 239
Kzo9I GATC 1 cut(s) 92
LguI GCTCTTC 1 cut(s) 44
LpnPI CCDG 8 cut(s) 26, 170, 210, 232, 241, 251, 292, 319
Lsp1109I GCAGC 1 cut(s) 253
LweI GCATC 3 cut(s) 46, 94, 116
MaeI CTAG 1 cut(s) 191
MalI GATC 1 cut(s) 94
MboI GATC 1 cut(s) 92
MboII GAAGA 1 cut(s) 61
MflI RGATCY 1 cut(s) 92
MlyI GAGTC 1 cut(s) 296
MnlI CCTC 7 cut(s) 66, 118, 211, 220, 226, 235, 264
Mph1103I ATGCAT 1 cut(s) 109
MroNI GCCGGC 1 cut(s) 237
MslI CAYNNNNRTG 2 cut(s) 112, 331
MspI CCGG 4 cut(s) 219, 228, 238, 329
MspR9I CCNGG 3 cut(s) 14, 229, 307
MvaI CCWGG 2 cut(s) 14, 307
MvnI CGCG 1 cut(s) 251
MwoI GCNNNNNNNGC 2 cut(s) 206, 238
NaeI GCCGGC 1 cut(s) 239
NciI CCSGG 1 cut(s) 229
NdeI CATATG 1 cut(s) 98
NdeII GATC 1 cut(s) 92
NgoMIV GCCGGC 1 cut(s) 237
NlaIII CATG 1 cut(s) 117
NlaIV GGNNCC 2 cut(s) 94, 223
NsiI ATGCAT 1 cut(s) 109
OliI CACNNNNGTG 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 44
PdiI GCCGGC 1 cut(s) 239
PfeI GAWTC 1 cut(s) 9
PflMI CCANNNNNTGG 1 cut(s) 167
PkrI GCNGC 2 cut(s) 243, 253
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
Psp6I CCWGG 2 cut(s) 12, 305
PspFI CCCAGC 1 cut(s) 196
PspGI CCWGG 2 cut(s) 12, 305
PspN4I GGNNCC 2 cut(s) 94, 223
PspPI GGNCC 2 cut(s) 117, 194
PsuI RGATCY 1 cut(s) 92
RsaI GTAC 5 cut(s) 5, 41, 89, 176, 248
RsaNI GTAC 5 cut(s) 4, 40, 88, 175, 247
RseI CAYNNNNRTG 2 cut(s) 112, 331
SapI GCTCTTC 1 cut(s) 44
SatI GCNGC 2 cut(s) 242, 252
Sau3AI GATC 1 cut(s) 92
Sau96I GGNCC 2 cut(s) 117, 194
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 3 cut(s) 14, 229, 307
SetI ASST 4 cut(s) 41, 176, 227, 285
SfaNI GCATC 3 cut(s) 46, 94, 116
SmiMI CAYNNNNRTG 2 cut(s) 112, 331
SsiI CCGC 1 cut(s) 251
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 3 cut(s) 12, 227, 305
TatI WGTACW 1 cut(s) 3
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 1 cut(s) 9
TseI GCWGC 1 cut(s) 241
TspDTI ATGAA 1 cut(s) 154
TspGWI ACGGA 2 cut(s) 105, 193
Van91I CCANNNNNTGG 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
XmiI GTMKAC 1 cut(s) 285
XspI CTAG 1 cut(s) 191
Zsp2I ATGCAT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.