Rh5DG453500

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0032818
Physical Location & Seq
Reverse (-)
72297746 .. 72300034
2289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG453500.1

Sequence Viewer

Length: 936 bp
ATGAAGTGTTTCCATTTCACCAACGGCGAGAGGCGCGACGACGACGACACCGAGCCCGGCGTCGTTTCTCGCGCGCCGTCCAAGGTCTCCTGGGCGCGCTCCCTCAGCGTCGCCTCCAGCAGCTACGACAACACCAACTACAATCGCCGCTCCGAGTTCGACTCCGACTCCCGGGACATGTCCGACTCCGCGGCCTTCTACGACTTGCTGAGTCAGCGCCGGGCCAATGATCTGAGGGTGTTCGCCTTCTCCGAGCTCAAATCGGCCAGCCGCGGGTTCAGTCGGGCCTTGCTGATCGGCGAGGGAGGGTTTGGGTGCGTTTACCGAGGGATCGTTAACGACGACGGAGCTAAAATGGACGTCGCTATCAAACAGTTGAATCGGTATGCAGCTCCAGAATATGTACAGACAGGGAAACTTACCGCTAAAAGTGATGTCTGGAGCTTTGGAGTGGTTCTCTACGAGCTCATCACAGGAAGGAGGGCGATGGAGAGAAATCTGCCCCGGAGTGAGCAAAAACTTTTGGAATGGGTCAGACCTTATGCTTCAGACGCGAAGAAATTCCACCTTATTGTAGACCCACGACTTGAAGGACAGTATTGCATCAAATCGGCTCAGAAGCTGGCATCGTTAGCTAACAGGTGTCTATCAAAACAACCAAAGTCTCGCCCGAAAATGAGTGACGTGGTTGGGATTTTGGGAACTATCATCGATGAGCAAACATCTCAAGATGAAGTTGCCCCTCAACCTGTTATCGAAACTGACGAGGTGAGGGAAGAAACTTTGGAGGTGGCTGAGATTGAATCTACTACCAAACAAGGAACCAAGAATCTGAAAAAGGTTTTCGATATAAGAGATATGGTAAACTTTAGAAACAAATCTATCGGGAAGTTAGATTGGAGAAACTGGACCCCTGGGTTGATACGAACTTGGTGA

Protein Analysis

311

Amino Acids

35.26

Weight (kDa)

8.74

Isoelectric Point (pI)

47.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 128 - 231 4.8e-12 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 128 - 230 2.3e-11 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0032818)

Species Orthologous Gene IDs
rosa_samantha Rh5AG425500 Rh5DG453500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 363
AccBSI CCGCTC 1 cut(s) 150
AccI GTMKAC 1 cut(s) 576
AccII CGCG 7 cut(s) 36, 72, 74, 97, 191, 273, 554
AciI CCGC 6 cut(s) 148, 189, 191, 271, 273, 423
AclWI GGATC 1 cut(s) 338
AcoI YGGCCR 1 cut(s) 264
AcsI RAATTY 1 cut(s) 560
AcuI CTGAAG 1 cut(s) 531
AcyI GRCGYC 2 cut(s) 60, 360
AfaI GTAC 1 cut(s) 405
AfiI CCNNNNNNNGG 2 cut(s) 171, 273
AflIII ACRYGT 1 cut(s) 177
AgsI TTSAA 3 cut(s) 379, 590, 803
AjiI CACGTC 1 cut(s) 685
AjnI CCWGG 2 cut(s) 89, 913
AluBI AGCT 8 cut(s) 123, 256, 350, 392, 444, 466, 622, 635
AluI AGCT 8 cut(s) 123, 256, 350, 392, 444, 466, 622, 635
Alw21I GWGCWC 2 cut(s) 258, 468
Alw26I GTCTC 2 cut(s) 91, 669
AlwI GGATC 1 cut(s) 338
AlwNI CAGNNNCTG 1 cut(s) 622
Ama87I CYCGRG 1 cut(s) 171
AoxI GGCC 4 cut(s) 192, 222, 264, 285
ApeKI GCWGC 2 cut(s) 120, 389
ApoI RAATTY 1 cut(s) 560
Asp700I GAANNNNTTC 2 cut(s) 8, 560
AspLEI GCGC 6 cut(s) 36, 74, 76, 97, 99, 219
AspS9I GGNCC 3 cut(s) 222, 285, 909
AsuC2I CCSGG 5 cut(s) 57, 172, 173, 221, 505
AsuHPI GGTGA 2 cut(s) 10, 781
AvaI CYCGRG 1 cut(s) 171
AvaII GGWCC 1 cut(s) 909
BanII GRGCYC 3 cut(s) 57, 258, 468
Bbv12I GWGCWC 2 cut(s) 258, 468
BbvCI CCTCAGC 1 cut(s) 104
BbvI GCAGC 2 cut(s) 132, 401
BccI CCATC 1 cut(s) 481
BceAI ACGGC 2 cut(s) 40, 61
BciT130I CCWGG 2 cut(s) 91, 915
BcnI CCSGG 5 cut(s) 57, 172, 173, 221, 505
BcoDI GTCTC 2 cut(s) 91, 669
BfoI RGCGCY 1 cut(s) 220
BisI GCNGC 5 cut(s) 121, 148, 192, 271, 390
BlsI GCNGC 5 cut(s) 122, 149, 193, 272, 391
Bme1390I CCNGG 7 cut(s) 57, 91, 172, 173, 221, 505, 915
Bme18I GGWCC 1 cut(s) 909
BmeT110I CYCGRG 1 cut(s) 171
BmgBI CACGTC 1 cut(s) 685
BmgT120I GGNCC 3 cut(s) 222, 285, 909
BmiI GGNNCC 2 cut(s) 823, 911
BmrFI CCNGG 7 cut(s) 57, 91, 172, 173, 221, 505, 915
BmsI GCATC 2 cut(s) 612, 635
BplI GAGNNNNNCTC 4 cut(s) 146, 178, 441, 473
BpmI CTGGAG 3 cut(s) 100, 378, 460
Bpu10I CCTNAGC 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 711
BpuMI CCSGG 5 cut(s) 57, 172, 173, 221, 505
Bsa29I ATCGAT 1 cut(s) 711
BsaHI GRCGYC 2 cut(s) 60, 360
BsaI GGTCTC 1 cut(s) 91
BsaJI CCNNGG 9 cut(s) 81, 90, 171, 189, 271, 325, 503, 913, 914
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 273
Bse1I ACTGG 1 cut(s) 911
BseBI CCWGG 2 cut(s) 91, 915
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 9 cut(s) 81, 90, 171, 189, 271, 325, 503, 913, 914
BseLI CCNNNNNNNGG 2 cut(s) 171, 273
BseMII CTCAG 5 cut(s) 118, 200, 224, 629, 786
BseNI ACTGG 1 cut(s) 911
BsePI GCGCGC 2 cut(s) 72, 95
BseXI GCAGC 2 cut(s) 132, 401
Bsh1236I CGCG 7 cut(s) 36, 72, 74, 97, 191, 273, 554
BshFI GGCC 4 cut(s) 194, 224, 266, 287
BshVI ATCGAT 1 cut(s) 711
BsiHKAI GWGCWC 2 cut(s) 258, 468
BsiHKCI CYCGRG 1 cut(s) 171
BsiSI CCGG 4 cut(s) 57, 172, 220, 505
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 2 cut(s) 171, 273
BsmAI GTCTC 2 cut(s) 91, 669
BsmFI GGGAC 1 cut(s) 188
BsnI GGCC 4 cut(s) 194, 224, 266, 287
Bso31I GGTCTC 1 cut(s) 91
BsoBI CYCGRG 1 cut(s) 171
Bsp1286I GDGCHC 3 cut(s) 57, 258, 468
Bsp1407I TGTACA 1 cut(s) 403
Bsp143I GATC 3 cut(s) 229, 294, 330
BspACI CCGC 6 cut(s) 148, 189, 191, 271, 273, 423
BspANI GGCC 4 cut(s) 194, 224, 266, 287
BspCNI CTCAG 5 cut(s) 117, 201, 225, 628, 787
BspDI ATCGAT 1 cut(s) 711
BspFNI CGCG 7 cut(s) 36, 72, 74, 97, 191, 273, 554
BspLI GGNNCC 2 cut(s) 823, 911
BspPI GGATC 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 91
BsrBI CCGCTC 1 cut(s) 150
BsrGI TGTACA 1 cut(s) 403
BsrI ACTGG 1 cut(s) 911
BssECI CCNNGG 9 cut(s) 81, 90, 171, 189, 271, 325, 503, 913, 914
BssHII GCGCGC 2 cut(s) 72, 95
BssMI GATC 3 cut(s) 229, 294, 330
BssNI GRCGYC 2 cut(s) 60, 360
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 2 cut(s) 91, 915
Bst4CI ACNGT 2 cut(s) 375, 597
BstACI GRCGYC 2 cut(s) 60, 360
BstAUI TGTACA 1 cut(s) 403
BstC8I GCNNGC 4 cut(s) 74, 97, 268, 624
BstDEI CTNAG 5 cut(s) 104, 209, 233, 615, 795
BstDSI CCRYGG 2 cut(s) 189, 271
BstFNI CGCG 7 cut(s) 36, 72, 74, 97, 191, 273, 554
BstH2I RGCGCY 1 cut(s) 220
BstHHI GCGC 6 cut(s) 36, 74, 76, 97, 99, 219
BstKTI GATC 3 cut(s) 232, 297, 333
BstMAI GTCTC 2 cut(s) 91, 669
BstMBI GATC 3 cut(s) 229, 294, 330
BstMWI GCNNNNNNNGC 5 cut(s) 33, 105, 214, 551, 632
BstNI CCWGG 2 cut(s) 91, 915
BstNSI RCATGY 1 cut(s) 181
BstSCI CCNGG 7 cut(s) 55, 89, 170, 171, 219, 503, 913
BstUI CGCG 7 cut(s) 36, 72, 74, 97, 191, 273, 554
BstV1I GCAGC 2 cut(s) 132, 401
Bsu15I ATCGAT 1 cut(s) 711
BsuRI GGCC 4 cut(s) 194, 224, 266, 287
BsuTUI ATCGAT 1 cut(s) 711
BtgI CCRYGG 2 cut(s) 189, 271
BtgZI GCGATG 1 cut(s) 500
BtrI CACGTC 1 cut(s) 685
Cac8I GCNNGC 4 cut(s) 74, 97, 268, 624
CaiI CAGNNNCTG 1 cut(s) 622
CfoI GCGC 6 cut(s) 36, 74, 76, 97, 99, 219
Cfr13I GGNCC 3 cut(s) 222, 285, 909
Cfr42I CCGCGG 2 cut(s) 192, 274
Cfr9I CCCGGG 1 cut(s) 171
ClaI ATCGAT 1 cut(s) 711
CseI GACGC 3 cut(s) 49, 97, 560
Csp6I GTAC 1 cut(s) 404
CviAII CATG 1 cut(s) 178
CviQI GTAC 1 cut(s) 404
DdeI CTNAG 5 cut(s) 104, 209, 233, 615, 795
DpnI GATC 3 cut(s) 231, 296, 332
DpnII GATC 3 cut(s) 229, 294, 330
EaeI YGGCCR 1 cut(s) 264
Ecl136II GAGCTC 2 cut(s) 256, 466
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 3 cut(s) 57, 258, 468
Eco31I GGTCTC 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 909
Eco53kI GAGCTC 2 cut(s) 256, 466
Eco57I CTGAAG 1 cut(s) 531
Eco88I CYCGRG 1 cut(s) 171
EcoICRI GAGCTC 2 cut(s) 256, 466
EcoRII CCWGG 2 cut(s) 89, 913
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 3 cut(s) 57, 258, 468
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 1 cut(s) 181
FaiI YATR 6 cut(s) 179, 387, 402, 543, 851, 860
FaqI GGGAC 1 cut(s) 188
FatI CATG 1 cut(s) 177
FauI CCCGC 1 cut(s) 266
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 5 cut(s) 121, 148, 192, 271, 390
FriOI GRGCYC 3 cut(s) 57, 258, 468
Fsp4HI GCNGC 5 cut(s) 121, 148, 192, 271, 390
GlaI GCGC 6 cut(s) 35, 73, 75, 96, 98, 218
GluI GCNGC 5 cut(s) 121, 148, 192, 271, 390
GsuI CTGGAG 3 cut(s) 100, 378, 460
HaeII RGCGCY 1 cut(s) 220
HaeIII GGCC 4 cut(s) 194, 224, 266, 287
HapII CCGG 4 cut(s) 57, 172, 220, 505
HgaI GACGC 3 cut(s) 49, 97, 560
HhaI GCGC 6 cut(s) 36, 74, 76, 97, 99, 219
Hin1I GRCGYC 2 cut(s) 60, 360
Hin1II CATG 1 cut(s) 181
Hin6I GCGC 6 cut(s) 34, 72, 74, 95, 97, 217
HinP1I GCGC 6 cut(s) 34, 72, 74, 95, 97, 217
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 7 cut(s) 161, 167, 185, 211, 379, 803, 829
HpaI GTTAAC 1 cut(s) 337
HpaII CCGG 4 cut(s) 57, 172, 220, 505
HphI GGTGA 2 cut(s) 10, 781
Hpy166II GTNNAC 4 cut(s) 322, 337, 577, 865
Hpy188I TCNGA 9 cut(s) 154, 166, 184, 234, 253, 536, 550, 618, 834
Hpy188III TCNNGA 4 cut(s) 395, 439, 728, 886
Hpy8I GTNNAC 4 cut(s) 322, 337, 577, 865
Hpy99I CGWCG 8 cut(s) 41, 44, 47, 65, 113, 344, 347, 365
HpyAV CCTTC 4 cut(s) 205, 256, 471, 584
HpyCH4III ACNGT 2 cut(s) 375, 597
HpyCH4IV ACGT 2 cut(s) 360, 684
HpyCH4V TGCA 2 cut(s) 389, 603
HpyF10VI GCNNNNNNNGC 5 cut(s) 33, 105, 214, 551, 632
HpyF3I CTNAG 5 cut(s) 104, 209, 233, 615, 795
HpySE526I ACGT 2 cut(s) 360, 684
Hsp92I GRCGYC 2 cut(s) 60, 360
Hsp92II CATG 1 cut(s) 181
HspAI GCGC 6 cut(s) 34, 72, 74, 95, 97, 217
KspAI GTTAAC 1 cut(s) 337
KspI CCGCGG 2 cut(s) 192, 274
Kzo9I GATC 3 cut(s) 229, 294, 330
LmnI GCTCC 5 cut(s) 104, 155, 347, 397, 441
Lsp1109I GCAGC 2 cut(s) 132, 401
LweI GCATC 2 cut(s) 612, 635
MaeII ACGT 2 cut(s) 360, 684
MaeIII GTNAC 1 cut(s) 680
MalI GATC 3 cut(s) 231, 296, 332
MbiI CCGCTC 1 cut(s) 150
MboI GATC 3 cut(s) 229, 294, 330
MboII GAAGA 2 cut(s) 568, 788
MhlI GDGCHC 3 cut(s) 57, 258, 468
MluCI AATT 1 cut(s) 560
MlyI GAGTC 4 cut(s) 155, 161, 179, 220
MmeI TCCRAC 2 cut(s) 189, 207
MroXI GAANNNNTTC 2 cut(s) 8, 560
MseI TTAA 1 cut(s) 336
MspA1I CMGCKG 2 cut(s) 191, 273
MspI CCGG 4 cut(s) 57, 172, 220, 505
MspR9I CCNGG 7 cut(s) 57, 91, 172, 173, 221, 505, 915
MvaI CCWGG 2 cut(s) 91, 915
MvnI CGCG 7 cut(s) 36, 72, 74, 97, 191, 273, 554
MwoI GCNNNNNNNGC 5 cut(s) 33, 105, 214, 551, 632
NciI CCSGG 5 cut(s) 57, 172, 173, 221, 505
NdeII GATC 3 cut(s) 229, 294, 330
NlaIII CATG 1 cut(s) 181
NlaIV GGNNCC 2 cut(s) 823, 911
NmuCI GTSAC 1 cut(s) 680
NspI RCATGY 1 cut(s) 181
PasI CCCWGGG 1 cut(s) 914
PauI GCGCGC 2 cut(s) 72, 95
PciI ACATGT 1 cut(s) 177
PcsI WCGNNNNNNNCGW 4 cut(s) 48, 249, 339, 762
PdmI GAANNNNTTC 2 cut(s) 8, 560
PfeI GAWTC 3 cut(s) 379, 803, 829
PkrI GCNGC 5 cut(s) 122, 149, 193, 272, 391
PleI GAGTC 4 cut(s) 155, 161, 179, 219
PpsI GAGTC 4 cut(s) 155, 161, 179, 219
PscI ACATGT 1 cut(s) 177
Psp124BI GAGCTC 2 cut(s) 258, 468
Psp6I CCWGG 2 cut(s) 89, 913
PspGI CCWGG 2 cut(s) 89, 913
PspN4I GGNNCC 2 cut(s) 823, 911
PspPI GGNCC 3 cut(s) 222, 285, 909
PstNI CAGNNNCTG 1 cut(s) 622
PteI GCGCGC 2 cut(s) 72, 95
RsaI GTAC 1 cut(s) 405
RsaNI GTAC 1 cut(s) 404
SacI GAGCTC 2 cut(s) 258, 468
SacII CCGCGG 2 cut(s) 192, 274
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 5 cut(s) 121, 148, 192, 271, 390
Sau3AI GATC 3 cut(s) 229, 294, 330
Sau96I GGNCC 3 cut(s) 222, 285, 909
SchI GAGTC 4 cut(s) 155, 161, 179, 220
ScrFI CCNGG 7 cut(s) 57, 91, 172, 173, 221, 505, 915
SduI GDGCHC 3 cut(s) 57, 258, 468
SfaNI GCATC 2 cut(s) 612, 635
Sfr303I CCGCGG 2 cut(s) 192, 274
SgrBI CCGCGG 2 cut(s) 192, 274
SinI GGWCC 1 cut(s) 909
SmaI CCCGGG 1 cut(s) 173
SmlI CTYRAG 1 cut(s) 726
SmoI CTYRAG 1 cut(s) 726
Sse9I AATT 1 cut(s) 560
SsiI CCGC 6 cut(s) 148, 189, 191, 271, 273, 423
SstI GAGCTC 2 cut(s) 258, 468
StyD4I CCNGG 7 cut(s) 55, 89, 170, 171, 219, 503, 913
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 2 cut(s) 375, 597
TaiI ACGT 2 cut(s) 363, 687
TaqI TCGA 4 cut(s) 159, 711, 756, 846
TasI AATT 1 cut(s) 560
TatI WGTACW 1 cut(s) 403
TauI GCSGC 3 cut(s) 150, 194, 273
TfiI GAWTC 3 cut(s) 379, 803, 829
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TseFI GTSAC 1 cut(s) 680
TseI GCWGC 2 cut(s) 120, 389
Tsp45I GTSAC 1 cut(s) 680
TspDTI ATGAA 2 cut(s) 17, 747
TspGWI ACGGA 1 cut(s) 360
TspMI CCCGGG 1 cut(s) 171
VpaK11BI GGWCC 1 cut(s) 909
XapI RAATTY 1 cut(s) 560
XceI RCATGY 1 cut(s) 181
XmaI CCCGGG 1 cut(s) 171
XmiI GTMKAC 1 cut(s) 576
XmnI GAANNNNTTC 2 cut(s) 8, 560
ZraI GACGTC 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.