Rh5DG559600

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0026173
Physical Location & Seq
Reverse (-)
86376900 .. 86395454
18555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG559600.1

Sequence Viewer

Length: 888 bp
ATGCCTCAACCAAGGCACACCCAGGCAGTTGAGATCCAAATGAAGGACCTCGTTCCCAGCCTTCCAGAACTGCAGATCTGGCACCCCCCGACGAACGCCGCTACCGCCAATCACAACCCCACCACGATCAACCTCACATCGACACCAGCAGCCGACAGACACCAGTTCATCCACCAGATCAGAACCGGCAAACAAACCTGTCCACACCATCCACGAGCACAGCAAAACACCAGCCCCAACAAGAGGAAACAGAGAGAAGAAAAGCAGGCCAAGACCTGCAGATTTATCCATGGAGAGGAGAAGCTCAATACAGAAATCCAACCAAGCTCGACAACTCCGGGCCTGTCTCCGGCTTCTCCGATTGATCCTAAAGGAGTTCATTGGAGACCTCTCTATATTCTTTCAAATGCCTTGGAGCGGCAAGTCACCCCTGGGGATAGGACTCGAGATTTCAGATGGGAGAAGAAAGGGTTGGCTTTCTGTCTTCAAACTGAAAGGGAGAGATTTCCCTCGTTTGGGGGTTCATATTTCAGACTCTGCTCAATCTACATTAATGGTGTGTGCATCAACAAAGAATCCAAACCCTTGCGAAGCCGTTGTTCCGGTAAGCATTTGTGGTTGTGCTATGTGCCATTCAATACAATAATAAGACGGCTCAGTGAGAGTGGCTTACCGCCACCTTCCATTTGTCTGGTTGAGTTTGAATTTGAATATCAGACCCCAAAAAATCATGAGGATTTCGTGAAACAAATTCTAGTAAGGGAGGGAAGCTGCGGGGTTCACGTAGTAATGACAGAGGATGAAGGGGTATTTGTGAATTCTCCATCATCTTCAGGAACCATCAAATCCTTAGAAAGTGGAAGGACTGCTACTCTCGGCCTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

295

Amino Acids

33.4

Weight (kDa)

9.16

Isoelectric Point (pI)

59.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026173)

Species Orthologous Gene IDs
rosa_samantha Rh1AG126300 Rh1CG120200 Rh5DG559600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 284
AccB1I GGYRCC 1 cut(s) 81
AccBSI CCGCTC 1 cut(s) 418
AciI CCGC 5 cut(s) 99, 105, 418, 674, 774
AclWI GGATC 2 cut(s) 28, 359
AcsI RAATTY 3 cut(s) 704, 750, 817
AcuI CTGAAG 1 cut(s) 816
AfiI CCNNNNNNNGG 7 cut(s) 43, 243, 295, 349, 417, 515, 516
AgsI TTSAA 5 cut(s) 405, 488, 637, 704, 710
AjnI CCWGG 2 cut(s) 21, 430
AjuI GAANNNNNNNTTGG 2 cut(s) 455, 487
AluBI AGCT 3 cut(s) 304, 327, 771
AluI AGCT 3 cut(s) 304, 327, 771
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 2 cut(s) 351, 379
AlwI GGATC 2 cut(s) 28, 359
AlwNI CAGNNNCTG 1 cut(s) 537
Ama87I CYCGRG 1 cut(s) 444
AoxI GGCC 3 cut(s) 267, 340, 877
ApeKI GCWGC 2 cut(s) 149, 771
ApoI RAATTY 3 cut(s) 704, 750, 817
AseI ATTAAT 1 cut(s) 552
AspS9I GGNCC 2 cut(s) 46, 340
AsuC2I CCSGG 1 cut(s) 339
AsuHPI GGTGA 1 cut(s) 418
AvaI CYCGRG 1 cut(s) 444
AvaII GGWCC 1 cut(s) 46
BanI GGYRCC 1 cut(s) 81
BarI GAAGNNNNNNTAC 2 cut(s) 853, 885
BauI CACGAG 1 cut(s) 213
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 2 cut(s) 161, 758
BccI CCATC 4 cut(s) 216, 450, 832, 848
BceAI ACGGC 2 cut(s) 579, 668
BciT130I CCWGG 2 cut(s) 23, 432
BcnI CCSGG 1 cut(s) 339
BcoDI GTCTC 2 cut(s) 351, 379
BfaI CTAG 1 cut(s) 755
BfmI CTRYAG 2 cut(s) 71, 277
BfuAI ACCTGC 1 cut(s) 284
BglII AGATCT 1 cut(s) 75
BisI GCNGC 4 cut(s) 99, 150, 419, 772
BlsI GCNGC 4 cut(s) 100, 151, 420, 773
Bme1390I CCNGG 3 cut(s) 23, 339, 432
Bme18I GGWCC 1 cut(s) 46
BmeT110I CYCGRG 1 cut(s) 444
BmgT120I GGNCC 2 cut(s) 46, 340
BmiI GGNNCC 2 cut(s) 83, 838
BmrFI CCNGG 3 cut(s) 23, 339, 432
BmsI GCATC 1 cut(s) 573
BpiI GAAGAC 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 339
BsaAI YACGTR 1 cut(s) 784
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 6 cut(s) 11, 21, 289, 411, 430, 431
BsaWI WCCGGW 1 cut(s) 602
Bsc4I CCNNNNNNNGG 7 cut(s) 43, 243, 295, 349, 417, 515, 516
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 1 cut(s) 163
BseBI CCWGG 2 cut(s) 23, 432
BseDI CCNNGG 6 cut(s) 11, 21, 289, 411, 430, 431
BseGI GGATG 3 cut(s) 168, 208, 805
BseLI CCNNNNNNNGG 7 cut(s) 43, 243, 295, 349, 417, 515, 516
BseMII CTCAG 1 cut(s) 670
BseNI ACTGG 1 cut(s) 163
BseRI GAGGAG 1 cut(s) 311
BseXI GCAGC 2 cut(s) 161, 758
BseYI CCCAGC 1 cut(s) 56
BshFI GGCC 3 cut(s) 269, 342, 879
BshNI GGYRCC 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 444
BsiSI CCGG 4 cut(s) 186, 338, 350, 603
BslI CCNNNNNNNGG 7 cut(s) 43, 243, 295, 349, 417, 515, 516
BsmAI GTCTC 2 cut(s) 351, 379
BsnI GGCC 3 cut(s) 269, 342, 879
Bso31I GGTCTC 1 cut(s) 379
BsoBI CYCGRG 1 cut(s) 444
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 5 cut(s) 33, 75, 126, 177, 364
Bsp19I CCATGG 1 cut(s) 289
BspACI CCGC 5 cut(s) 99, 105, 418, 674, 774
BspANI GGCC 3 cut(s) 269, 342, 879
BspCNI CTCAG 1 cut(s) 669
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 2 cut(s) 83, 838
BspMAI CTGCAG 2 cut(s) 75, 281
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 2 cut(s) 28, 359
BspT107I GGYRCC 1 cut(s) 81
BspTNI GGTCTC 1 cut(s) 379
BsrBI CCGCTC 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 1 cut(s) 163
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 6 cut(s) 11, 21, 289, 411, 430, 431
BssMI GATC 5 cut(s) 33, 75, 126, 177, 364
BssSI CACGAG 1 cut(s) 213
BssT1I CCWWGG 3 cut(s) 11, 289, 411
Bst2BI CACGAG 1 cut(s) 213
Bst2UI CCWGG 2 cut(s) 23, 432
BstBAI YACGTR 1 cut(s) 784
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 2 cut(s) 656, 850
BstDSI CCRYGG 1 cut(s) 289
BstF5I GGATG 3 cut(s) 168, 208, 805
BstKTI GATC 5 cut(s) 36, 78, 129, 180, 367
BstMAI GTCTC 2 cut(s) 351, 379
BstMBI GATC 5 cut(s) 33, 75, 126, 177, 364
BstMWI GCNNNNNNNGC 2 cut(s) 79, 104
BstNI CCWGG 2 cut(s) 23, 432
BstSCI CCNGG 3 cut(s) 21, 337, 430
BstSFI CTRYAG 2 cut(s) 71, 277
BstV1I GCAGC 2 cut(s) 161, 758
BstV2I GAAGAC 1 cut(s) 476
BstX2I RGATCY 2 cut(s) 33, 75
BstXI CCANNNNNNTGG 1 cut(s) 691
BstYI RGATCY 2 cut(s) 33, 75
BsuRI GGCC 3 cut(s) 269, 342, 879
BtgI CCRYGG 1 cut(s) 289
BtsCI GGATG 3 cut(s) 168, 208, 805
BtsIMutI CAGTG 1 cut(s) 664
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 1 cut(s) 267
CaiI CAGNNNCTG 1 cut(s) 537
CciI TCATGA 1 cut(s) 730
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 2 cut(s) 46, 340
CviAII CATG 2 cut(s) 290, 731
DdeI CTNAG 2 cut(s) 656, 850
DpnI GATC 5 cut(s) 35, 77, 128, 179, 366
DpnII GATC 5 cut(s) 33, 75, 126, 177, 364
Eco130I CCWWGG 3 cut(s) 11, 289, 411
Eco31I GGTCTC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 816
Eco88I CYCGRG 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 46
EcoRI GAATTC 1 cut(s) 817
EcoRII CCWGG 2 cut(s) 21, 430
EcoT14I CCWWGG 3 cut(s) 11, 289, 411
ErhI CCWWGG 3 cut(s) 11, 289, 411
FaeI CATG 2 cut(s) 293, 734
FaiI YATR 5 cut(s) 291, 396, 526, 627, 732
FatI CATG 2 cut(s) 289, 730
FauI CCCGC 1 cut(s) 767
Fnu4HI GCNGC 4 cut(s) 99, 150, 419, 772
FokI GGATG 3 cut(s) 155, 195, 812
Fsp4HI GCNGC 4 cut(s) 99, 150, 419, 772
FspBI CTAG 1 cut(s) 755
GluI GCNGC 4 cut(s) 99, 150, 419, 772
GsaI CCCAGC 1 cut(s) 60
HaeIII GGCC 3 cut(s) 269, 342, 879
HapII CCGG 4 cut(s) 186, 338, 350, 603
Hin1II CATG 2 cut(s) 293, 734
HinfI GANTC 3 cut(s) 442, 534, 575
HpaII CCGG 4 cut(s) 186, 338, 350, 603
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 2 cut(s) 203, 781
Hpy188I TCNGA 5 cut(s) 182, 360, 455, 533, 717
Hpy188III TCNNGA 5 cut(s) 65, 446, 731, 742, 834
Hpy8I GTNNAC 2 cut(s) 203, 781
Hpy99I CGWCG 1 cut(s) 94
HpyAV CCTTC 5 cut(s) 37, 71, 690, 797, 855
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 3 cut(s) 73, 279, 564
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 104
HpyF3I CTNAG 2 cut(s) 656, 850
HpySE526I ACGT 1 cut(s) 783
Hsp92II CATG 2 cut(s) 293, 734
Kzo9I GATC 5 cut(s) 33, 75, 126, 177, 364
LmnI GCTCC 1 cut(s) 415
Lsp1109I GCAGC 2 cut(s) 161, 758
LweI GCATC 1 cut(s) 573
MaeI CTAG 1 cut(s) 755
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 1 cut(s) 424
MalI GATC 5 cut(s) 35, 77, 128, 179, 366
MbiI CCGCTC 1 cut(s) 418
MboI GATC 5 cut(s) 33, 75, 126, 177, 364
MboII GAAGA 4 cut(s) 269, 475, 476, 822
MflI RGATCY 2 cut(s) 33, 75
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 3 cut(s) 704, 750, 817
MlyI GAGTC 2 cut(s) 436, 528
MmeI TCCRAC 1 cut(s) 343
MseI TTAA 2 cut(s) 552, 886
MspI CCGG 4 cut(s) 186, 338, 350, 603
MspR9I CCNGG 3 cut(s) 23, 339, 432
MvaI CCWGG 2 cut(s) 23, 432
MwoI GCNNNNNNNGC 2 cut(s) 79, 104
NciI CCSGG 1 cut(s) 339
NcoI CCATGG 1 cut(s) 289
NdeII GATC 5 cut(s) 33, 75, 126, 177, 364
NlaIII CATG 2 cut(s) 293, 734
NlaIV GGNNCC 2 cut(s) 83, 838
NmeAIII GCCGAG 1 cut(s) 855
NmuCI GTSAC 1 cut(s) 424
PaeR7I CTCGAG 1 cut(s) 444
PagI TCATGA 1 cut(s) 730
PasI CCCWGGG 1 cut(s) 431
PfeI GAWTC 1 cut(s) 575
PkrI GCNGC 4 cut(s) 100, 151, 420, 773
PleI GAGTC 2 cut(s) 436, 528
PpsI GAGTC 2 cut(s) 436, 528
Ppu21I YACGTR 1 cut(s) 784
PpuMI RGGWCCY 1 cut(s) 46
PshBI ATTAAT 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 46
Psp6I CCWGG 2 cut(s) 21, 430
PspFI CCCAGC 1 cut(s) 56
PspGI CCWGG 2 cut(s) 21, 430
PspN4I GGNNCC 2 cut(s) 83, 838
PspPI GGNCC 2 cut(s) 46, 340
PspPPI RGGWCCY 1 cut(s) 46
PstI CTGCAG 2 cut(s) 75, 281
PstNI CAGNNNCTG 1 cut(s) 537
PsuI RGATCY 2 cut(s) 33, 75
SaqAI TTAA 2 cut(s) 552, 886
SatI GCNGC 4 cut(s) 99, 150, 419, 772
Sau3AI GATC 5 cut(s) 33, 75, 126, 177, 364
Sau96I GGNCC 2 cut(s) 46, 340
SchI GAGTC 2 cut(s) 436, 528
ScrFI CCNGG 3 cut(s) 23, 339, 432
SduI GDGCHC 1 cut(s) 220
SfaNI GCATC 1 cut(s) 573
SfcI CTRYAG 2 cut(s) 71, 277
Sfr274I CTCGAG 1 cut(s) 444
SinI GGWCC 1 cut(s) 46
SlaI CTCGAG 1 cut(s) 444
SmlI CTYRAG 1 cut(s) 444
SmoI CTYRAG 1 cut(s) 444
Sse9I AATT 3 cut(s) 704, 750, 817
SsiI CCGC 5 cut(s) 99, 105, 418, 674, 774
SspMI CTAG 1 cut(s) 755
StyD4I CCNGG 3 cut(s) 21, 337, 430
StyI CCWWGG 3 cut(s) 11, 289, 411
TaiI ACGT 1 cut(s) 786
TaqI TCGA 3 cut(s) 140, 329, 445
TasI AATT 3 cut(s) 704, 750, 817
TauI GCSGC 2 cut(s) 101, 421
TfiI GAWTC 1 cut(s) 575
Tru1I TTAA 2 cut(s) 552, 886
Tru9I TTAA 2 cut(s) 552, 886
TscAI CASTG 1 cut(s) 664
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 2 cut(s) 149, 771
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 5 cut(s) 56, 157, 368, 513, 816
TspRI CASTG 1 cut(s) 664
VpaK11BI GGWCC 1 cut(s) 46
VspI ATTAAT 1 cut(s) 552
XapI RAATTY 3 cut(s) 704, 750, 817
XhoI CTCGAG 1 cut(s) 444
XspI CTAG 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.