Rh6AG471500

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0032432
Physical Location & Seq
Reverse (-)
65410946 .. 65415921
4976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG471500.1

Sequence Viewer

Length: 1173 bp
ATGCGATTGCATCGATCATCTGAATTCCTATATAATAGTGGTTTCAAGGTTGAACATGTGCGTTCTGAGAGAAATTTGCTTGCTGAGGTTGATAGTCGGTGTATTGTGAAACTTTATTATTCATTTCAAGATTCCGATTTCTTATACCTTATCATGGAGTATTTACCCGGTGGTGACATTATGACATTATTGATGAGAGAAGATACTCTTTCTGAAGATGTTGCACGTTTTTATATGGGAGAGAGTATTCTGGCTATTCACTCAATCCATCAACACAACTATGTTCACAGGGACATAAAACCAGACAATCTGATACTGGATAAAACTGGCCATTTGAAGCTTTCAGATTTTGGCTTGTGTAAACCTCTGGATGACAAGTATTCTACATTATTGGAAAATGAGGATTTAACATCTCAAGAATGCATAGCTGAAACTGAAGGACAATCTGGCTGTGATAAGATTCCTTGGTTGATGCCAAAAGAACAATTACAACAATGGAAACGTAATCGGCGTGCCTTGGCGTATTCAACTGTTGGAACTCTTGACTACATGGCACCCGAGGTTTTGCTGAAGAAAGGATATGGTATGGAGTGTGATTGGTGGTCACTAGGAGCAATCATGTACGAGATGCTAGTAGGCTATCCTCCATTCTGCTCTGATGATCCAAGGATCACATGCCGCAAGATTATCAATTGGAGAACATGCCTGAAGTTTCCCGACGAGCCAAAAATTTCAGACGAGGCAAGGGATTTGATCTGTCACTTGTTATGTGATGTTGAAACAAGGCTTGGGACACATGGAGTAGAAGAAATTAAGGCACATCCTTGGTTCAGAGGTATTGAGTGGGACAGGCTATATGAAATTGAAGCTGCGTATAAACCTACAGTTGTTGGAGACTTGGACACTCAGAATTTTGAGAAGTTTCCTGATCTAGAAGGTGCACCATCTGCAACACCAACAGTGGGCCCTTGGCGGAAGATGTTAACCTCGAAAGATAACAATTTCATGGGATACACTTTCAAGAAATCAGATGTCCTTAAATCACTTGAAAGTTCAGGTACAGACATGAGATCAAATGGATCTTCAAAGTCCCCATCACTGATTTCCTTGTTAGGTAGAATTGACTTGCAAGACAGTGTAATACCAGAGGGCGAACCAAAGCAGGAAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000902 GO:0000904 GO:0002009 GO:0002165 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007423 GO:0007444 GO:0007455 GO:0007469 GO:0007472 GO:0007476 GO:0007552 GO:0007560 GO:0008037 GO:0008038 GO:0008144 GO:0008150 GO:0008152 GO:0008544 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009913 GO:0009987 GO:0010769 GO:0010975 GO:0016043 GO:0016301 GO:0016310 GO:0016358 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022008 GO:0022416 GO:0022603 GO:0022604 GO:0023052 GO:0030030 GO:0030154 GO:0030182 GO:0030424 GO:0030425 GO:0030554 GO:0030855 GO:0031175 GO:0031344 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0032990 GO:0035107 GO:0035114 GO:0035120 GO:0035214 GO:0035220 GO:0035239 GO:0035295 GO:0035315 GO:0035316 GO:0035317 GO:0035556 GO:0035639 GO:0036094 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044424 GO:0044444 GO:0044463 GO:0044464 GO:0045595 GO:0045664 GO:0048468 GO:0048513 GO:0048563 GO:0048569 GO:0048666 GO:0048667 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048800 GO:0048812 GO:0048813 GO:0048814 GO:0048856 GO:0048858 GO:0048869 GO:0050767 GO:0050773 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051239 GO:0051716 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0065007 GO:0070451 GO:0070593 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0097447 GO:0097458 GO:0099568 GO:0120025 GO:0120035 GO:0120036 GO:0120038 GO:0120039 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

390

Amino Acids

44.77

Weight (kDa)

5.16

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 18 - 130 6.5e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 18 - 134 1.9e-15 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 49 - 128 7e-07 ABC1 atypical kinase-like domain
Pkinase PF00069 174 - 277 2.1e-21 Protein kinase domain
Pkinase_C PF00433 297 - 340 6.2e-07 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0032432)

Species Orthologous Gene IDs
rosa_rugosa Rorug06G0362900
rosa_samantha Rh6AG471500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 553
AciI CCGC 2 cut(s) 679, 973
AclWI GGATC 3 cut(s) 656, 677, 1087
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 4 cut(s) 23, 73, 729, 910
AcuI CTGAAG 4 cut(s) 234, 456, 590, 728
AfaI GTAC 2 cut(s) 623, 1060
AfiI CCNNNNNNNGG 2 cut(s) 154, 962
AflIII ACRYGT 1 cut(s) 55
AjuI GAANNNNNNNTTGG 2 cut(s) 771, 803
AluBI AGCT 3 cut(s) 340, 428, 869
AluI AGCT 3 cut(s) 340, 428, 869
Alw21I GWGCWC 1 cut(s) 943
Alw26I GTCTC 1 cut(s) 888
Alw44I GTGCAC 1 cut(s) 939
AlwI GGATC 3 cut(s) 656, 677, 1087
Ama87I CYCGRG 1 cut(s) 557
AoxI GGCC 2 cut(s) 328, 964
ApaI GGGCCC 1 cut(s) 968
ApaLI GTGCAC 1 cut(s) 939
ApeKI GCWGC 1 cut(s) 869
ApoI RAATTY 4 cut(s) 23, 73, 729, 910
AspS9I GGNCC 2 cut(s) 964, 965
AsuC2I CCSGG 1 cut(s) 168
AsuHPI GGTGA 1 cut(s) 185
AvaI CYCGRG 1 cut(s) 557
BaeGI GKGCMC 2 cut(s) 943, 968
BalI TGGCCA 1 cut(s) 330
BanI GGYRCC 1 cut(s) 553
BanII GRGCYC 1 cut(s) 968
Bbv12I GWGCWC 1 cut(s) 943
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 856
BccI CCATC 3 cut(s) 276, 952, 1102
BciVI GTATCC 1 cut(s) 1004
BcnI CCSGG 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 888
BfaI CTAG 3 cut(s) 608, 632, 932
BfmI CTRYAG 1 cut(s) 882
BfuI GTATCC 1 cut(s) 1004
BisI GCNGC 2 cut(s) 679, 870
BlsI GCNGC 2 cut(s) 680, 871
Bme1390I CCNGG 1 cut(s) 168
BmeT110I CYCGRG 1 cut(s) 557
BmgT120I GGNCC 2 cut(s) 964, 965
BmiI GGNNCC 2 cut(s) 555, 966
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 3 cut(s) 19, 462, 618
Bpu10I CCTNAGC 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 399
BpuMI CCSGG 1 cut(s) 168
Bsa29I ATCGAT 1 cut(s) 13
BsaJI CCNNGG 6 cut(s) 464, 516, 558, 665, 824, 968
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 962
Bse1I ACTGG 2 cut(s) 321, 331
BseCI ATCGAT 1 cut(s) 13
BseDI CCNNGG 6 cut(s) 464, 516, 558, 665, 824, 968
BseGI GGATG 2 cut(s) 376, 820
BseLI CCNNNNNNNGG 2 cut(s) 154, 962
BseMII CTCAG 3 cut(s) 57, 75, 920
BseNI ACTGG 2 cut(s) 321, 331
BseSI GKGCMC 2 cut(s) 943, 968
BseXI GCAGC 1 cut(s) 856
BshFI GGCC 2 cut(s) 330, 966
BshNI GGYRCC 1 cut(s) 553
BshVI ATCGAT 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 943
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 1 cut(s) 168
BslFI GGGAC 4 cut(s) 305, 805, 860, 1075
BslI CCNNNNNNNGG 2 cut(s) 154, 962
BsmAI GTCTC 1 cut(s) 888
BsmFI GGGAC 4 cut(s) 305, 805, 860, 1075
BsmI GAATGC 1 cut(s) 425
BsnI GGCC 2 cut(s) 330, 966
BsoBI CYCGRG 1 cut(s) 557
Bsp120I GGGCCC 1 cut(s) 964
Bsp1286I GDGCHC 2 cut(s) 943, 968
Bsp143I GATC 7 cut(s) 14, 661, 669, 753, 928, 1070, 1079
BspACI CCGC 2 cut(s) 679, 973
BspANI GGCC 2 cut(s) 330, 966
BspCNI CTCAG 3 cut(s) 58, 76, 919
BspDI ATCGAT 1 cut(s) 13
BspLI GGNNCC 2 cut(s) 555, 966
BspPI GGATC 3 cut(s) 656, 677, 1087
BspT107I GGYRCC 1 cut(s) 553
BsrI ACTGG 2 cut(s) 321, 331
BssECI CCNNGG 6 cut(s) 464, 516, 558, 665, 824, 968
BssMI GATC 7 cut(s) 14, 661, 669, 753, 928, 1070, 1079
BssT1I CCWWGG 5 cut(s) 464, 516, 665, 824, 968
Bst4CI ACNGT 4 cut(s) 532, 886, 961, 1136
BstAPI GCANNNNNTGC 1 cut(s) 947
BstC8I GCNNGC 2 cut(s) 81, 513
BstDEI CTNAG 3 cut(s) 66, 84, 906
BstF5I GGATG 2 cut(s) 376, 820
BstKTI GATC 7 cut(s) 17, 664, 672, 756, 931, 1073, 1082
BstMAI GTCTC 1 cut(s) 888
BstMBI GATC 7 cut(s) 14, 661, 669, 753, 928, 1070, 1079
BstMWI GCNNNNNNNGC 1 cut(s) 947
BstNSI RCATGY 3 cut(s) 59, 678, 705
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 882
BstSLI GKGCMC 2 cut(s) 943, 968
BstV1I GCAGC 1 cut(s) 856
BstX2I RGATCY 1 cut(s) 1079
BstYI RGATCY 1 cut(s) 1079
Bsu15I ATCGAT 1 cut(s) 13
BsuI GTATCC 1 cut(s) 1004
BsuRI GGCC 2 cut(s) 330, 966
BsuTUI ATCGAT 1 cut(s) 13
BtsCI GGATG 2 cut(s) 376, 820
BtsIMutI CAGTG 3 cut(s) 966, 1097, 1141
Cac8I GCNNGC 2 cut(s) 81, 513
Cfr13I GGNCC 2 cut(s) 964, 965
ClaI ATCGAT 1 cut(s) 13
Csp6I GTAC 2 cut(s) 622, 1059
CviAII CATG 9 cut(s) 56, 154, 550, 619, 675, 702, 797, 1006, 1066
CviQI GTAC 2 cut(s) 622, 1059
DdeI CTNAG 3 cut(s) 66, 84, 906
DpnI GATC 7 cut(s) 16, 663, 671, 755, 930, 1072, 1081
DpnII GATC 7 cut(s) 14, 661, 669, 753, 928, 1070, 1079
EaeI YGGCCR 1 cut(s) 328
EciI GGCGGA 1 cut(s) 988
Eco130I CCWWGG 5 cut(s) 464, 516, 665, 824, 968
Eco24I GRGCYC 1 cut(s) 968
Eco57I CTGAAG 4 cut(s) 234, 456, 590, 728
Eco88I CYCGRG 1 cut(s) 557
EcoO109I RGGNCCY 1 cut(s) 965
EcoRI GAATTC 1 cut(s) 23
EcoT14I CCWWGG 5 cut(s) 464, 516, 665, 824, 968
EcoT22I ATGCAT 1 cut(s) 425
EcoT38I GRGCYC 1 cut(s) 968
ErhI CCWWGG 5 cut(s) 464, 516, 665, 824, 968
FaeI CATG 9 cut(s) 59, 157, 553, 622, 678, 705, 800, 1009, 1069
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FaqI GGGAC 4 cut(s) 305, 805, 860, 1075
FatI CATG 9 cut(s) 55, 153, 549, 618, 674, 701, 796, 1005, 1065
Fnu4HI GCNGC 2 cut(s) 679, 870
FokI GGATG 2 cut(s) 383, 807
FriOI GRGCYC 1 cut(s) 968
Fsp4HI GCNGC 2 cut(s) 679, 870
FspBI CTAG 3 cut(s) 608, 632, 932
GluI GCNGC 2 cut(s) 679, 870
HaeIII GGCC 2 cut(s) 330, 966
HapII CCGG 1 cut(s) 168
Hin1II CATG 9 cut(s) 59, 157, 553, 622, 678, 705, 800, 1009, 1069
HincII GTYRAC 1 cut(s) 984
HindII GTYRAC 1 cut(s) 984
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 2 cut(s) 131, 460
HpaI GTTAAC 1 cut(s) 984
HpaII CCGG 1 cut(s) 168
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 4 cut(s) 286, 362, 941, 984
Hpy188III TCNNGA 8 cut(s) 128, 368, 416, 542, 716, 926, 932, 1021
Hpy8I GTNNAC 4 cut(s) 286, 362, 941, 984
Hpy99I CGWCG 1 cut(s) 722
HpyAV CCTTC 3 cut(s) 431, 929, 1160
HpyCH4III ACNGT 4 cut(s) 532, 886, 961, 1136
HpyCH4IV ACGT 2 cut(s) 226, 502
HpyCH4V TGCA 6 cut(s) 10, 224, 423, 941, 950, 1129
HpyF10VI GCNNNNNNNGC 1 cut(s) 947
HpyF3I CTNAG 3 cut(s) 66, 84, 906
HpySE526I ACGT 2 cut(s) 226, 502
Hsp92II CATG 9 cut(s) 59, 157, 553, 622, 678, 705, 800, 1009, 1069
KspAI GTTAAC 1 cut(s) 984
Kzo9I GATC 7 cut(s) 14, 661, 669, 753, 928, 1070, 1079
LmnI GCTCC 1 cut(s) 611
Lsp1109I GCAGC 1 cut(s) 856
LweI GCATC 3 cut(s) 19, 462, 618
MaeI CTAG 3 cut(s) 608, 632, 932
MaeII ACGT 2 cut(s) 226, 502
MaeIII GTNAC 3 cut(s) 173, 603, 758
MalI GATC 7 cut(s) 16, 663, 671, 755, 930, 1072, 1081
MboI GATC 7 cut(s) 14, 661, 669, 753, 928, 1070, 1079
MboII GAAGA 6 cut(s) 212, 227, 583, 818, 988, 1074
MfeI CAATTG 1 cut(s) 691
MflI RGATCY 1 cut(s) 1079
MhlI GDGCHC 2 cut(s) 943, 968
MlsI TGGCCA 1 cut(s) 330
MluNI TGGCCA 1 cut(s) 330
MmeI TCCRAC 2 cut(s) 514, 871
MnlI CCTC 9 cut(s) 79, 375, 394, 553, 654, 733, 827, 997, 1141
Mox20I TGGCCA 1 cut(s) 330
Mph1103I ATGCAT 1 cut(s) 425
MscI TGGCCA 1 cut(s) 330
MseI TTAA 4 cut(s) 407, 813, 983, 1038
MslI CAYNNNNRTG 1 cut(s) 279
Msp20I TGGCCA 1 cut(s) 330
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 168
MunI CAATTG 1 cut(s) 691
Mva1269I GAATGC 1 cut(s) 425
MwoI GCNNNNNNNGC 1 cut(s) 947
NciI CCSGG 1 cut(s) 168
NdeII GATC 7 cut(s) 14, 661, 669, 753, 928, 1070, 1079
NlaIII CATG 9 cut(s) 59, 157, 553, 622, 678, 705, 800, 1009, 1069
NlaIV GGNNCC 2 cut(s) 555, 966
NmuCI GTSAC 3 cut(s) 173, 603, 758
NsiI ATGCAT 1 cut(s) 425
NspI RCATGY 3 cut(s) 59, 678, 705
PciI ACATGT 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 508
PctI GAATGC 1 cut(s) 425
PfeI GAWTC 2 cut(s) 131, 460
PkrI GCNGC 2 cut(s) 680, 871
PscI ACATGT 1 cut(s) 55
PspN4I GGNNCC 2 cut(s) 555, 966
PspOMI GGGCCC 1 cut(s) 964
PspPI GGNCC 2 cut(s) 964, 965
PsuI RGATCY 1 cut(s) 1079
RsaI GTAC 2 cut(s) 623, 1060
RsaNI GTAC 2 cut(s) 622, 1059
RseI CAYNNNNRTG 1 cut(s) 279
SaqAI TTAA 4 cut(s) 407, 813, 983, 1038
SatI GCNGC 2 cut(s) 679, 870
Sau3AI GATC 7 cut(s) 14, 661, 669, 753, 928, 1070, 1079
Sau96I GGNCC 2 cut(s) 964, 965
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 2 cut(s) 943, 968
SfaNI GCATC 3 cut(s) 19, 462, 618
SfcI CTRYAG 1 cut(s) 882
SmiMI CAYNNNNRTG 1 cut(s) 279
SmlI CTYRAG 1 cut(s) 414
SmoI CTYRAG 1 cut(s) 414
SsiI CCGC 2 cut(s) 679, 973
SspMI CTAG 3 cut(s) 608, 632, 932
StyD4I CCNGG 1 cut(s) 166
StyI CCWWGG 5 cut(s) 464, 516, 665, 824, 968
TaaI ACNGT 4 cut(s) 532, 886, 961, 1136
TaiI ACGT 2 cut(s) 229, 505
TaqI TCGA 2 cut(s) 13, 989
TauI GCSGC 1 cut(s) 681
TfiI GAWTC 2 cut(s) 131, 460
Tru1I TTAA 4 cut(s) 407, 813, 983, 1038
Tru9I TTAA 4 cut(s) 407, 813, 983, 1038
TscAI CASTG 3 cut(s) 966, 1104, 1141
TseFI GTSAC 3 cut(s) 173, 603, 758
TseI GCWGC 1 cut(s) 869
Tsp45I GTSAC 3 cut(s) 173, 603, 758
TspDTI ATGAA 3 cut(s) 111, 873, 994
TspRI CASTG 3 cut(s) 966, 1104, 1141
VneI GTGCAC 1 cut(s) 939
XapI RAATTY 4 cut(s) 23, 73, 729, 910
XbaI TCTAGA 1 cut(s) 931
XceI RCATGY 3 cut(s) 59, 678, 705
XspI CTAG 3 cut(s) 608, 632, 932
Zsp2I ATGCAT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.