Rh6DG204400

Vacuolar iron transporter homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0030310
Physical Location & Seq
Reverse (-)
36368189 .. 36368794
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG204400.1

Sequence Viewer

Length: 606 bp
ATGGCGCCAAATAGCCAAGAATCCGTAAATGGGGGCAAGTACCCTAACCCAACCATGACCAACCTGGACCTCGAGCACCAACCCACCCAAGACCTCAATACTTCCCAGGACAACGACGACTTCGACTACTCCAAACGCTCCCAGTGGCTTCGTGCCGCCGTCCTGGGAGCCAACGACGGTCTGATCTCCACCGCGTCTTTAATGATGGGTGTTGGTGCTGTTAAGCAGGACATTAAAGCCATGATACTAACCGGGTTTGCCGGACTAGTAGCCGGTGCCTGCAGCATGGCCATCGGAGAGTTTGTCTCCGTTTACTCCCAGTTGGACATAGAGGTGGCCCAAATGAAGAGAGACAACAACAAAAACAACAAGCAAAAGAGCCCGGTGGTACACGTGGTAGAAGACGAGGAGGGAGAAGAGAAGGAGAAATTGCCAAACCCGTTACGAGCAGACGAGGAGGGAGAAGAGAAGGAGAACTTGCCAAACCCGTTACGAGCAGCAGGTGCATCGGCTCTTGCGTTTTCAGTGGGAGCAATGGTACCACTGCTAGCAGCCTCGTTCATAAGGGAGTATAGGGCGAGGTTGGGAGTGTGGCGGCGGCAGTGA

Protein Analysis

201

Amino Acids

21.92

Weight (kDa)

4.95

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VIT1 PF01988 50 - 189 5.3e-31 VIT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030310)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0275811
rosa_samantha Rh6DG204400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 491
Acc36I ACCTGC 1 cut(s) 491
Acc65I GGTACC 1 cut(s) 538
AccB1I GGYRCC 3 cut(s) 4, 275, 538
AccII CGCG 1 cut(s) 194
AciI CCGC 4 cut(s) 156, 192, 595, 598
AcoI YGGCCR 1 cut(s) 288
AcvI CACGTG 1 cut(s) 394
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 41, 390, 540
AfiI CCNNNNNNNGG 1 cut(s) 30
AflIII ACRYGT 1 cut(s) 391
AhlI ACTAGT 1 cut(s) 265
AjnI CCWGG 3 cut(s) 63, 105, 162
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 2 cut(s) 310, 345
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 2 cut(s) 288, 336
ApeKI GCWGC 3 cut(s) 282, 497, 551
Asp718I GGTACC 1 cut(s) 538
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 67, 337
AsuC2I CCSGG 2 cut(s) 253, 383
AsuNHI GCTAGC 1 cut(s) 547
AvaI CYCGRG 1 cut(s) 71
AvaII GGWCC 1 cut(s) 67
BalI TGGCCA 1 cut(s) 290
BanI GGYRCC 3 cut(s) 4, 275, 538
BanII GRGCYC 1 cut(s) 383
BbrPI CACGTG 1 cut(s) 394
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 3 cut(s) 294, 509, 563
BccI CCATC 2 cut(s) 199, 299
BceAI ACGGC 1 cut(s) 143
BcgI CGANNNNNNTGC 4 cut(s) 274, 308, 489, 523
BciT130I CCWGG 3 cut(s) 65, 107, 164
BcnI CCSGG 2 cut(s) 253, 383
BcoDI GTCTC 2 cut(s) 310, 345
BcuI ACTAGT 1 cut(s) 265
BfaI CTAG 2 cut(s) 266, 548
BfmI CTRYAG 1 cut(s) 280
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 491
BisI GCNGC 6 cut(s) 156, 283, 498, 552, 596, 599
BlsI GCNGC 6 cut(s) 157, 284, 499, 553, 597, 600
Bme1390I CCNGG 5 cut(s) 65, 107, 164, 253, 383
Bme18I GGWCC 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 2 cut(s) 67, 337
BmiI GGNNCC 4 cut(s) 6, 169, 277, 540
BmrFI CCNGG 5 cut(s) 65, 107, 164, 253, 383
BmrI ACTGGG 2 cut(s) 136, 313
BmsI GCATC 1 cut(s) 515
BmtI GCTAGC 1 cut(s) 551
BmuI ACTGGG 2 cut(s) 136, 313
BpiI GAAGAC 1 cut(s) 408
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpuMI CCSGG 2 cut(s) 253, 383
BsaAI YACGTR 1 cut(s) 394
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 105, 163
BsaXI ACNNNNNCTCC 4 cut(s) 288, 318, 522, 552
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 2 cut(s) 142, 319
Bse3DI GCAATG 1 cut(s) 540
BseBI CCWGG 3 cut(s) 65, 107, 164
BseDI CCNNGG 2 cut(s) 105, 163
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMI GCAATG 1 cut(s) 540
BseNI ACTGG 2 cut(s) 142, 319
BseRI GAGGAG 2 cut(s) 422, 470
BseXI GCAGC 3 cut(s) 294, 509, 563
Bsh1236I CGCG 1 cut(s) 194
BshFI GGCC 2 cut(s) 290, 338
BshNI GGYRCC 3 cut(s) 4, 275, 538
BsiHKAI GWGCWC 1 cut(s) 78
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 4 cut(s) 252, 261, 273, 383
BslI CCNNNNNNNGG 1 cut(s) 30
BsmAI GTCTC 2 cut(s) 310, 345
BsnI GGCC 2 cut(s) 290, 338
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 78, 383
Bsp143I GATC 1 cut(s) 183
BspACI CCGC 4 cut(s) 156, 192, 595, 598
BspANI GGCC 2 cut(s) 290, 338
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 4 cut(s) 6, 169, 277, 540
BspMAI CTGCAG 1 cut(s) 284
BspMI ACCTGC 1 cut(s) 491
BspOI GCTAGC 1 cut(s) 551
BspT107I GGYRCC 3 cut(s) 4, 275, 538
BsrDI GCAATG 1 cut(s) 540
BsrFI RCCGGY 1 cut(s) 272
BsrI ACTGG 2 cut(s) 142, 319
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 2 cut(s) 105, 163
BssMI GATC 1 cut(s) 183
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 3 cut(s) 65, 107, 164
Bst4CI ACNGT 1 cut(s) 179
Bst6I CTCTTC 3 cut(s) 341, 411, 459
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 503
BstBAI YACGTR 1 cut(s) 394
BstC8I GCNNGC 2 cut(s) 280, 549
BstFNI CGCG 1 cut(s) 194
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 1 cut(s) 186
BstMAI GTCTC 2 cut(s) 310, 345
BstMBI GATC 1 cut(s) 183
BstMWI GCNNNNNNNGC 1 cut(s) 503
BstNI CCWGG 3 cut(s) 65, 107, 164
BstSCI CCNGG 5 cut(s) 63, 105, 162, 251, 381
BstSFI CTRYAG 1 cut(s) 280
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 3 cut(s) 294, 509, 563
BstV2I GAAGAC 1 cut(s) 408
BsuRI GGCC 2 cut(s) 290, 338
BtsI GCAGTG 1 cut(s) 542
BtsIMutI CAGTG 3 cut(s) 149, 531, 542
BveI ACCTGC 1 cut(s) 491
Cac8I GCNNGC 2 cut(s) 280, 549
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 2 cut(s) 67, 337
CseI GACGC 1 cut(s) 183
Csp6I GTAC 3 cut(s) 40, 389, 539
CviAII CATG 3 cut(s) 55, 241, 286
CviQI GTAC 3 cut(s) 40, 389, 539
DinI GGCGCC 1 cut(s) 6
DpnI GATC 1 cut(s) 185
DpnII GATC 1 cut(s) 183
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 3 cut(s) 341, 411, 459
EarI CTCTTC 3 cut(s) 341, 411, 459
Eco24I GRGCYC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 67
Eco72I CACGTG 1 cut(s) 394
Eco88I CYCGRG 1 cut(s) 71
EcoRII CCWGG 3 cut(s) 63, 105, 162
EcoT38I GRGCYC 1 cut(s) 383
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
FaeI CATG 3 cut(s) 58, 244, 289
FaiI YATR 6 cut(s) 56, 242, 287, 329, 563, 573
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FatI CATG 3 cut(s) 54, 240, 285
Fnu4HI GCNGC 6 cut(s) 156, 283, 498, 552, 596, 599
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 6 cut(s) 156, 283, 498, 552, 596, 599
FspBI CTAG 2 cut(s) 266, 548
GlaI GCGC 1 cut(s) 6
GluI GCNGC 6 cut(s) 156, 283, 498, 552, 596, 599
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 290, 338
HapII CCGG 4 cut(s) 252, 261, 273, 383
HgaI GACGC 1 cut(s) 183
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 58, 244, 289
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 1 cut(s) 20
HpaII CCGG 4 cut(s) 252, 261, 273, 383
Hpy166II GTNNAC 2 cut(s) 313, 391
Hpy188I TCNGA 2 cut(s) 183, 296
Hpy8I GTNNAC 2 cut(s) 313, 391
Hpy99I CGWCG 2 cut(s) 119, 179
HpyAV CCTTC 2 cut(s) 415, 463
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 2 cut(s) 282, 506
HpyF10VI GCNNNNNNNGC 1 cut(s) 503
HpySE526I ACGT 1 cut(s) 393
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 58, 244, 289
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 1 cut(s) 542
Kzo9I GATC 1 cut(s) 183
LmnI GCTCC 3 cut(s) 143, 167, 530
Lsp1109I GCAGC 3 cut(s) 294, 509, 563
LweI GCATC 1 cut(s) 515
MaeI CTAG 2 cut(s) 266, 548
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 2 cut(s) 441, 489
MalI GATC 1 cut(s) 185
MboI GATC 1 cut(s) 183
MboII GAAGA 4 cut(s) 358, 413, 428, 476
MhlI GDGCHC 2 cut(s) 78, 383
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 1 cut(s) 428
MluNI TGGCCA 1 cut(s) 290
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 9 cut(s) 80, 104, 325, 400, 403, 448, 451, 565, 573
Mox20I TGGCCA 1 cut(s) 290
MscI TGGCCA 1 cut(s) 290
MseI TTAA 3 cut(s) 200, 222, 234
MslI CAYNNNNRTG 1 cut(s) 332
Msp20I TGGCCA 1 cut(s) 290
MspI CCGG 4 cut(s) 252, 261, 273, 383
MspR9I CCNGG 5 cut(s) 65, 107, 164, 253, 383
MvaI CCWGG 3 cut(s) 65, 107, 164
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 1 cut(s) 503
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 2 cut(s) 253, 383
NdeII GATC 1 cut(s) 183
NheI GCTAGC 1 cut(s) 547
NlaIII CATG 3 cut(s) 58, 244, 289
NlaIV GGNNCC 4 cut(s) 6, 169, 277, 540
PaeR7I CTCGAG 1 cut(s) 71
PaqCI CACCTGC 1 cut(s) 491
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 515
PfeI GAWTC 1 cut(s) 20
PkrI GCNGC 6 cut(s) 157, 284, 499, 553, 597, 600
PluTI GGCGCC 1 cut(s) 8
PmaCI CACGTG 1 cut(s) 394
PmlI CACGTG 1 cut(s) 394
Ppu21I YACGTR 1 cut(s) 394
Psp6I CCWGG 3 cut(s) 63, 105, 162
PspCI CACGTG 1 cut(s) 394
PspGI CCWGG 3 cut(s) 63, 105, 162
PspN4I GGNNCC 4 cut(s) 6, 169, 277, 540
PspPI GGNCC 2 cut(s) 67, 337
PspXI VCTCGAGB 1 cut(s) 71
PstI CTGCAG 1 cut(s) 284
RsaI GTAC 3 cut(s) 41, 390, 540
RsaNI GTAC 3 cut(s) 40, 389, 539
RseI CAYNNNNRTG 1 cut(s) 332
SaqAI TTAA 3 cut(s) 200, 222, 234
SatI GCNGC 6 cut(s) 156, 283, 498, 552, 596, 599
Sau3AI GATC 1 cut(s) 183
Sau96I GGNCC 2 cut(s) 67, 337
ScrFI CCNGG 5 cut(s) 65, 107, 164, 253, 383
SduI GDGCHC 2 cut(s) 78, 383
SetI ASST 7 cut(s) 66, 72, 96, 336, 396, 505, 584
SfaNI GCATC 1 cut(s) 515
SfcI CTRYAG 1 cut(s) 280
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 71
SinI GGWCC 1 cut(s) 67
SlaI CTCGAG 1 cut(s) 71
SmiMI CAYNNNNRTG 1 cut(s) 332
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SpeI ACTAGT 1 cut(s) 265
Sse9I AATT 1 cut(s) 428
SsiI CCGC 4 cut(s) 156, 192, 595, 598
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 2 cut(s) 266, 548
StyD4I CCNGG 5 cut(s) 63, 105, 162, 251, 381
TaaI ACNGT 1 cut(s) 179
TaiI ACGT 1 cut(s) 396
TaqI TCGA 2 cut(s) 72, 123
TasI AATT 1 cut(s) 428
TauI GCSGC 3 cut(s) 158, 598, 601
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 3 cut(s) 200, 222, 234
Tru9I TTAA 3 cut(s) 200, 222, 234
TscAI CASTG 3 cut(s) 149, 531, 549
TseI GCWGC 3 cut(s) 282, 497, 551
TspDTI ATGAA 2 cut(s) 359, 550
TspGWI ACGGA 2 cut(s) 13, 298
TspRI CASTG 3 cut(s) 149, 531, 549
VpaK11BI GGWCC 1 cut(s) 67
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 71
XspI CTAG 2 cut(s) 266, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.