Rw0G014040

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00628
OG0030219
Physical Location & Seq
Reverse (-)
25874 .. 26598
725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G014040.1

Sequence Viewer

Length: 459 bp
ATGAGCTCTCTGAAACCCATCTTGACTGACAAAAGATCTTCATCTGTTCAACTAGTGAGCTCGGAACTGGAAGAAACGCTATCCAACACCGAGCTTCCGTACTTCCACCGTCTTAGACGTGCCACGTGGGTCGAACTGAAGATTCTCTTCCGCCTGGCAGCTCCGGCAGTGGTGGTTTACTTGCTAAACAACGTTATTTCCACGTCCACCCAAATCTACTGCGGGCATCTCGGCAATCTCGAACTCGCTGCCTCTTCTCTTGGCAACACCGGCATCCAAGTTTTTGCCTACGGCCTCATGCTTGGAATGGGAAGTGCGGTGGAAACGCTCTGTGGTCAAGCCTATGGAGCAAAGAAATATGACATGCTAGGCGTATATCTGCAGCGTTCAACGATCCTGCTAATGGCAACTGGGATCCCAGTTATGTTCATCTTCATCTTCTCCGAGCCCATCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

152

Amino Acids

16.73

Weight (kDa)

7.77

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 55 - 151 9.1e-23 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030219)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0003361
rosa_wichuraiana Rw0G014040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 151, 222, 317
AclI AACGTT 1 cut(s) 192
AclWI GGATC 3 cut(s) 388, 409, 422
AcuI CTGAAG 1 cut(s) 158
AcvI CACGTG 1 cut(s) 126
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 403
AgsI TTSAA 2 cut(s) 50, 390
AhlI ACTAGT 1 cut(s) 52
AjiI CACGTC 2 cut(s) 119, 204
AjnI CCWGG 1 cut(s) 153
AluBI AGCT 4 cut(s) 6, 60, 94, 161
AluI AGCT 4 cut(s) 6, 60, 94, 161
Alw21I GWGCWC 2 cut(s) 8, 62
AlwI GGATC 3 cut(s) 388, 409, 422
AoxI GGCC 1 cut(s) 292
ApeKI GCWGC 3 cut(s) 158, 248, 382
BamHI GGATCC 1 cut(s) 414
BanII GRGCYC 3 cut(s) 8, 62, 450
BbrPI CACGTG 1 cut(s) 126
Bbv12I GWGCWC 2 cut(s) 8, 62
BbvI GCAGC 3 cut(s) 170, 235, 394
BccI CCATC 2 cut(s) 26, 458
BceAI ACGGC 1 cut(s) 307
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 1 cut(s) 155
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 2 cut(s) 53, 368
BfmI CTRYAG 1 cut(s) 380
BglII AGATCT 1 cut(s) 35
BisI GCNGC 3 cut(s) 159, 249, 383
BlsI GCNGC 3 cut(s) 160, 250, 384
Bme1390I CCNGG 1 cut(s) 155
BmgBI CACGTC 2 cut(s) 119, 204
BmiI GGNNCC 1 cut(s) 416
BmrFI CCNGG 1 cut(s) 155
BmrI ACTGGG 2 cut(s) 413, 420
BmsI GCATC 2 cut(s) 235, 282
BmuI ACTGGG 2 cut(s) 413, 420
BsaAI YACGTR 1 cut(s) 126
BsaBI GATNNNNATC 1 cut(s) 40
Bsc4I CCNNNNNNNGG 1 cut(s) 403
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 3 cut(s) 72, 415, 419
Bse8I GATNNNNATC 1 cut(s) 40
BseBI CCWGG 1 cut(s) 155
BseGI GGATG 1 cut(s) 273
BseJI GATNNNNATC 1 cut(s) 40
BseLI CCNNNNNNNGG 1 cut(s) 403
BseNI ACTGG 3 cut(s) 72, 415, 419
BseXI GCAGC 3 cut(s) 170, 235, 394
BshFI GGCC 1 cut(s) 294
BsiHKAI GWGCWC 2 cut(s) 8, 62
BsiSI CCGG 2 cut(s) 164, 270
BslI CCNNNNNNNGG 1 cut(s) 403
BsnI GGCC 1 cut(s) 294
Bsp1286I GDGCHC 3 cut(s) 8, 62, 450
Bsp143I GATC 3 cut(s) 35, 393, 414
BspACI CCGC 3 cut(s) 151, 222, 317
BspANI GGCC 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 416
BspMAI CTGCAG 1 cut(s) 384
BspPI GGATC 3 cut(s) 388, 409, 422
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 3 cut(s) 72, 415, 419
BssAI RCCGGY 1 cut(s) 269
BssMI GATC 3 cut(s) 35, 393, 414
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 1 cut(s) 110
Bst6I CTCTTC 2 cut(s) 152, 259
BstBAI YACGTR 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 224
BstDEI CTNAG 1 cut(s) 113
BstF5I GGATG 1 cut(s) 273
BstKTI GATC 3 cut(s) 38, 396, 417
BstMBI GATC 3 cut(s) 35, 393, 414
BstMWI GCNNNNNNNGC 3 cut(s) 164, 270, 347
BstNI CCWGG 1 cut(s) 155
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 153
BstSFI CTRYAG 1 cut(s) 380
BstV1I GCAGC 3 cut(s) 170, 235, 394
BstX2I RGATCY 2 cut(s) 35, 414
BstYI RGATCY 2 cut(s) 35, 414
BsuRI GGCC 1 cut(s) 294
BtrI CACGTC 2 cut(s) 119, 204
BtsCI GGATG 1 cut(s) 273
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 1 cut(s) 174
Cac8I GCNNGC 1 cut(s) 224
Cfr10I RCCGGY 1 cut(s) 269
Csp6I GTAC 1 cut(s) 100
CviAII CATG 2 cut(s) 298, 364
CviJI RGCY 7 cut(s) 6, 60, 94, 161, 294, 341, 448
CviKI_1 RGCY 7 cut(s) 6, 60, 94, 161, 294, 341, 448
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 1 cut(s) 113
DpnI GATC 3 cut(s) 37, 395, 416
DpnII GATC 3 cut(s) 35, 393, 414
Eam1104I CTCTTC 2 cut(s) 152, 259
EarI CTCTTC 2 cut(s) 152, 259
EciI GGCGGA 1 cut(s) 140
Ecl136II GAGCTC 2 cut(s) 6, 60
Eco24I GRGCYC 3 cut(s) 8, 62, 450
Eco53kI GAGCTC 2 cut(s) 6, 60
Eco57I CTGAAG 1 cut(s) 158
Eco72I CACGTG 1 cut(s) 126
EcoICRI GAGCTC 2 cut(s) 6, 60
EcoRII CCWGG 1 cut(s) 153
EcoT38I GRGCYC 3 cut(s) 8, 62, 450
FaeI CATG 2 cut(s) 301, 367
FaiI YATR 6 cut(s) 299, 345, 360, 365, 376, 425
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FatI CATG 2 cut(s) 297, 363
FauI CCCGC 1 cut(s) 215
Fnu4HI GCNGC 3 cut(s) 159, 249, 383
FokI GGATG 1 cut(s) 260
FriOI GRGCYC 3 cut(s) 8, 62, 450
Fsp4HI GCNGC 3 cut(s) 159, 249, 383
FspBI CTAG 2 cut(s) 53, 368
GluI GCNGC 3 cut(s) 159, 249, 383
HaeIII GGCC 1 cut(s) 294
HapII CCGG 2 cut(s) 164, 270
Hin1II CATG 2 cut(s) 301, 367
HinfI GANTC 1 cut(s) 142
HpaII CCGG 2 cut(s) 164, 270
Hpy166II GTNNAC 2 cut(s) 178, 207
Hpy188I TCNGA 3 cut(s) 12, 64, 445
Hpy188III TCNNGA 2 cut(s) 22, 239
Hpy8I GTNNAC 2 cut(s) 178, 207
HpyCH4III ACNGT 1 cut(s) 110
HpyCH4IV ACGT 4 cut(s) 118, 125, 192, 203
HpyCH4V TGCA 1 cut(s) 382
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 270, 347
HpyF3I CTNAG 1 cut(s) 113
HpySE526I ACGT 4 cut(s) 118, 125, 192, 203
Hsp92II CATG 2 cut(s) 301, 367
Kzo9I GATC 3 cut(s) 35, 393, 414
LmnI GCTCC 2 cut(s) 166, 347
LpnPI CCDG 8 cut(s) 53, 140, 167, 177, 283, 396, 410, 432
Lsp1109I GCAGC 3 cut(s) 170, 235, 394
LweI GCATC 2 cut(s) 235, 282
MaeI CTAG 2 cut(s) 53, 368
MaeII ACGT 4 cut(s) 118, 125, 192, 203
MalI GATC 3 cut(s) 37, 395, 416
MboI GATC 3 cut(s) 35, 393, 414
MboII GAAGA 7 cut(s) 30, 83, 139, 151, 246, 424, 430
MflI RGATCY 2 cut(s) 35, 414
MhlI GDGCHC 3 cut(s) 8, 62, 450
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 2 cut(s) 262, 305
MspI CCGG 2 cut(s) 164, 270
MspR9I CCNGG 1 cut(s) 155
MvaI CCWGG 1 cut(s) 155
MwoI GCNNNNNNNGC 3 cut(s) 164, 270, 347
NdeII GATC 3 cut(s) 35, 393, 414
NlaIII CATG 2 cut(s) 301, 367
NlaIV GGNNCC 1 cut(s) 416
NmeAIII GCCGAG 1 cut(s) 210
NspI RCATGY 1 cut(s) 367
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 1 cut(s) 142
PkrI GCNGC 3 cut(s) 160, 250, 384
PmaCI CACGTG 1 cut(s) 126
PmlI CACGTG 1 cut(s) 126
Ppu21I YACGTR 1 cut(s) 126
Psp124BI GAGCTC 2 cut(s) 8, 62
Psp1406I AACGTT 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 153
PspCI CACGTG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 416
PstI CTGCAG 1 cut(s) 384
PsuI RGATCY 2 cut(s) 35, 414
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SacI GAGCTC 2 cut(s) 8, 62
SatI GCNGC 3 cut(s) 159, 249, 383
Sau3AI GATC 3 cut(s) 35, 393, 414
ScrFI CCNGG 1 cut(s) 155
SduI GDGCHC 3 cut(s) 8, 62, 450
SetI ASST 8 cut(s) 8, 62, 96, 121, 128, 163, 195, 206
SfaNI GCATC 2 cut(s) 235, 282
SfcI CTRYAG 1 cut(s) 380
SpeI ACTAGT 1 cut(s) 52
SsiI CCGC 3 cut(s) 151, 222, 317
SspMI CTAG 2 cut(s) 53, 368
SstI GAGCTC 2 cut(s) 8, 62
StyD4I CCNGG 1 cut(s) 153
TaaI ACNGT 1 cut(s) 110
TaiI ACGT 4 cut(s) 121, 128, 195, 206
TaqI TCGA 2 cut(s) 132, 240
TfiI GAWTC 1 cut(s) 142
TscAI CASTG 1 cut(s) 174
TseI GCWGC 3 cut(s) 158, 248, 382
TspDTI ATGAA 3 cut(s) 30, 418, 424
TspGWI ACGGA 1 cut(s) 87
TspRI CASTG 1 cut(s) 174
XceI RCATGY 1 cut(s) 367
XspI CTAG 2 cut(s) 53, 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.