Rw2G018390

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0031205
Physical Location & Seq
Forward (+)
23687604 .. 23690636
3033 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G018390.1

Sequence Viewer

Length: 1776 bp
ATGTTTCCTAAGCTAGAAAGACTTGTACTAAATGATCTTCCAAACCTCTCTGGACTCAAAGCTGTAACAGTTCGACAATGCAATCGTCTCAAAAATATTCTTCCCGAATCAACCATAGCCATTGTTTTTCAGCACCTACAGAAGTTGACAGTGGAGCAATGTGAAGTCACGGAAATAGTTGCCCAGGAGGATGTACAAGATACATTAATGCCTGATCAGGTTGTCTTCCCAGATGTAACATGTTTGGAATTTAAAAGTCTGCTCCAACTTAAGAGCTTCTATCCAAGGACGCATGCTTTCAAGTGGCCCAAACTCAAAAAATTAGTGTTGTCTGACTGCCCGTACGTGGGAATTTTTGCCTCGGAATTTTTATGCCTTCAGAAATCACAGGACTTGCATTTCAGAAAAAGGAAATCAAAGGAGATGGGCGGTCAGTGTGCTCCAGTTAAACAATCTTTTGTTCTGATTGAGAAGGTTGTGGACACCTCAGAGTCTGCTAGTAGCCTCGGAACAAATCAGAACCAGTCTGTCTCGAATGCATCTGAAGCAGCTCAAAATGGCGCAAGAGAAATCCATGATGATGGAATTGTTTCTGAGCTGAACCATGACCAACAGGCCCTATTGGATGGCATTTCTGAAAGAGAAGGAGTTGAAGCTGTGATCCAAGGAAATCATGTCAGTTCTCAAAACATGAAACACAGTAAGATTATGGACCCAAATCAGGAGCATATAGCTGTCACGAATTCTCCAGAGAATTTTCAGGAGGCTGCCAATCTTCAAATGCAAGGGACTGTCATTCAACCTTTGAATGTTATTGTAACTTATCAAGGTTCCCTGCCCTCTGTTGTGATTGACGGTGTTGATGCCACCGTTAAAGAAGTTGTGTTTGCATCTGAGATTGAACAAGGGGTTAATTCAGATATCCTTCGGAGCGTCAGGGCAGATGGACCAACACATGGTGACACAGATGTGGTTTTAGAGCTTCCAGTCTGCTCAGGTGTAGATGCTTCTGAAGTGGATAATGCTATAATTCTTGGAACTGCACCCTCTATACAGGTTGTGGAAGCCTCTTCCGAGCAAAATCTACCTCTCCAAGTTCCCCATGAGCCTATAAAGGTGTTATCTAGTGCAGACAGACTGAAAAGGTTCGACACGGCTGCAGCTTTGCTTACCTGTGGTCAGTCGACCCAAAGTTCTCAAGAGACTGAAACAGCTAAGGTCGTAATATCAAGACTTTTTGGGAGTTCAGCGGACTTGCTTGAAAGTATAGAAGCTGAAAATCAATTCAAAGAGGCCTTGGAAATCCTAGTTACACAAAAAGTTATTCCTCCACCTATCCAGACCAGAACAATAACTTTCTTAAGAGATATTAAGGAAGCTAAAGAATCAGGTGCACTGGCTACAGAAAAGCTGGCCTCATTGAACCAAGACATTAAAAGGGAAAACTTGGTCAGATCAAAACTAGGCGAGCTTGCAAATGAAGGCAAGCAGTTTCAACTTCAAGCTGATTCAAGGTCTCAGCGCATACTTAAGCTTGATATGGAGATAAGTTCGGAAGAAGAAATGACCAAAGAAATAGAGTGGAGAGATCACGAGCAAAAGCTCGCTGACGAGAGACTTGCAACAATTGATAGTCTGACGCTTCAGGTAGCTAACTATCCAGATCGTCGTGCAGCCCTTGTTGACTGTCAAGCAGACAAAGATTTGGCTGATGGCAAAGTTGTATTCTTGTACAGCTCTATGAAACAACTTCTAGAGAAGAACCTCAATTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

591

Amino Acids

65.33

Weight (kDa)

4.95

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 16 - 114 2.5e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031205)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002351.1_g000051
rosa_wichuraiana Rw2G018390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 956
AccI GTMKAC 1 cut(s) 1184
AciI CCGC 2 cut(s) 429, 1250
AclWI GGATC 1 cut(s) 655
AcsI RAATTY 5 cut(s) 248, 351, 365, 742, 754
AcuI CTGAAG 4 cut(s) 362, 564, 1032, 1628
AdeI CACNNNGTG 1 cut(s) 959
AfaI GTAC 4 cut(s) 27, 195, 344, 1733
AfiI CCNNNNNNNGG 4 cut(s) 346, 347, 721, 956
AflII CTTAAG 3 cut(s) 269, 1360, 1529
AflIII ACRYGT 1 cut(s) 239
AjnI CCWGG 1 cut(s) 183
AleI CACNNNNGTG 1 cut(s) 968
Alw21I GWGCWC 2 cut(s) 442, 1396
Alw26I GTCTC 5 cut(s) 92, 535, 1196, 1521, 1609
Alw44I GTGCAC 1 cut(s) 1392
AlwI GGATC 1 cut(s) 655
AlwNI CAGNNNCTG 1 cut(s) 494
AoxI GGCC 4 cut(s) 305, 615, 1293, 1413
ApaLI GTGCAC 1 cut(s) 1392
ApeKI GCWGC 5 cut(s) 548, 767, 1157, 1160, 1673
ApoI RAATTY 5 cut(s) 248, 351, 365, 742, 754
AseI ATTAAT 1 cut(s) 206
Asp700I GAANNNNTTC 2 cut(s) 589, 1145
AspLEI GCGC 2 cut(s) 563, 1524
AspS9I GGNCC 4 cut(s) 306, 616, 712, 947
AsuHPI GGTGA 1 cut(s) 971
AvaII GGWCC 2 cut(s) 712, 947
BaeGI GKGCMC 1 cut(s) 1396
BauI CACGAG 1 cut(s) 1592
BbsI GAAGAC 1 cut(s) 217
Bbv12I GWGCWC 2 cut(s) 442, 1396
BbvI GCAGC 5 cut(s) 560, 754, 1144, 1172, 1685
BccI CCATC 5 cut(s) 418, 575, 620, 938, 1706
BceAI ACGGC 1 cut(s) 1170
BcgI CGANNNNNNTGC 4 cut(s) 1139, 1173, 1601, 1635
BciT130I CCWGG 1 cut(s) 185
BclI TGATCA 1 cut(s) 214
BcoDI GTCTC 5 cut(s) 92, 535, 1196, 1521, 1609
BfaI CTAG 6 cut(s) 14, 498, 1125, 1307, 1463, 1754
BfmI CTRYAG 3 cut(s) 137, 1158, 1401
BfrI CTTAAG 3 cut(s) 269, 1360, 1529
BisI GCNGC 5 cut(s) 549, 768, 1158, 1161, 1674
BlsI GCNGC 5 cut(s) 550, 769, 1159, 1162, 1675
Bme1390I CCNGG 1 cut(s) 185
Bme18I GGWCC 2 cut(s) 712, 947
BmgT120I GGNCC 4 cut(s) 306, 616, 712, 947
BmiI GGNNCC 2 cut(s) 714, 832
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 4 cut(s) 548, 853, 899, 994
BpiI GAAGAC 1 cut(s) 217
BpmI CTGGAG 2 cut(s) 426, 732
Bpu10I CCTNAGC 3 cut(s) 9, 994, 1215
BpuEI CTTGAG 1 cut(s) 1182
BsaAI YACGTR 1 cut(s) 346
BsaI GGTCTC 1 cut(s) 1521
BsaJI CCNNGG 6 cut(s) 183, 284, 360, 505, 664, 1296
BsaXI ACNNNNNCTCC 2 cut(s) 730, 760
Bsc4I CCNNNNNNNGG 4 cut(s) 346, 347, 721, 956
Bse1I ACTGG 4 cut(s) 443, 523, 986, 1401
Bse3DI GCAATG 1 cut(s) 164
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 6 cut(s) 183, 284, 360, 505, 664, 1296
BseGI GGATG 2 cut(s) 196, 631
BseLI CCNNNNNNNGG 4 cut(s) 346, 347, 721, 956
BseMI GCAATG 1 cut(s) 164
BseMII CTCAG 5 cut(s) 501, 585, 885, 1008, 1532
BseNI ACTGG 4 cut(s) 443, 523, 986, 1401
BseSI GKGCMC 1 cut(s) 1396
BseXI GCAGC 5 cut(s) 560, 754, 1144, 1172, 1685
BsgI GTGCAG 3 cut(s) 1026, 1149, 1692
BshFI GGCC 4 cut(s) 307, 617, 1295, 1415
BsiHKAI GWGCWC 2 cut(s) 442, 1396
BsiWI CGTACG 1 cut(s) 342
BslFI GGGAC 1 cut(s) 802
BslI CCNNNNNNNGG 4 cut(s) 346, 347, 721, 956
BsmAI GTCTC 5 cut(s) 92, 535, 1196, 1521, 1609
BsmBI CGTCTC 1 cut(s) 92
BsmFI GGGAC 1 cut(s) 802
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 4 cut(s) 307, 617, 1295, 1415
Bso31I GGTCTC 1 cut(s) 1521
Bsp1286I GDGCHC 2 cut(s) 442, 1396
Bsp1407I TGTACA 2 cut(s) 193, 1731
Bsp143I GATC 6 cut(s) 34, 214, 660, 1454, 1588, 1663
BspACI CCGC 2 cut(s) 429, 1250
BspANI GGCC 4 cut(s) 307, 617, 1295, 1415
BspCNI CTCAG 5 cut(s) 500, 586, 886, 1007, 1531
BspLI GGNNCC 2 cut(s) 714, 832
BspMAI CTGCAG 1 cut(s) 1162
BspPI GGATC 1 cut(s) 655
BspTI CTTAAG 3 cut(s) 269, 1360, 1529
BspTNI GGTCTC 1 cut(s) 1521
BsrDI GCAATG 1 cut(s) 164
BsrGI TGTACA 2 cut(s) 193, 1731
BsrI ACTGG 4 cut(s) 443, 523, 986, 1401
BssECI CCNNGG 6 cut(s) 183, 284, 360, 505, 664, 1296
BssMI GATC 6 cut(s) 34, 214, 660, 1454, 1588, 1663
BssSI CACGAG 1 cut(s) 1592
BssT1I CCWWGG 3 cut(s) 284, 664, 1296
Bst2BI CACGAG 1 cut(s) 1592
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 7 cut(s) 70, 151, 701, 793, 857, 871, 1688
Bst6I CTCTTC 1 cut(s) 1075
BstAFI CTTAAG 3 cut(s) 269, 1360, 1529
BstAUI TGTACA 2 cut(s) 193, 1731
BstBAI YACGTR 1 cut(s) 346
BstC8I GCNNGC 6 cut(s) 294, 1413, 1469, 1473, 1487, 1605
BstDEI CTNAG 7 cut(s) 9, 487, 594, 894, 994, 1215, 1518
BstF5I GGATG 2 cut(s) 196, 631
BstHHI GCGC 2 cut(s) 563, 1524
BstKTI GATC 6 cut(s) 37, 217, 663, 1457, 1591, 1666
BstMAI GTCTC 5 cut(s) 92, 535, 1196, 1521, 1609
BstMBI GATC 6 cut(s) 34, 214, 660, 1454, 1588, 1663
BstMWI GCNNNNNNNGC 1 cut(s) 545
BstNI CCWGG 1 cut(s) 185
BstNSI RCATGY 2 cut(s) 243, 296
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 3 cut(s) 137, 1158, 1401
BstSLI GKGCMC 1 cut(s) 1396
BstV1I GCAGC 5 cut(s) 560, 754, 1144, 1172, 1685
BstV2I GAAGAC 1 cut(s) 217
BstXI CCANNNNNNTGG 1 cut(s) 581
BsuRI GGCC 4 cut(s) 307, 617, 1295, 1415
BtsCI GGATG 2 cut(s) 196, 631
BtsIMutI CAGTG 3 cut(s) 156, 440, 1394
Cac8I GCNNGC 6 cut(s) 294, 1413, 1469, 1473, 1487, 1605
CaiI CAGNNNCTG 1 cut(s) 494
CfoI GCGC 2 cut(s) 563, 1524
Cfr13I GGNCC 4 cut(s) 306, 616, 712, 947
CseI GACGC 3 cut(s) 298, 922, 1648
Csp6I GTAC 4 cut(s) 26, 194, 343, 1732
CviAII CATG 8 cut(s) 240, 293, 575, 605, 674, 691, 956, 1103
CviQI GTAC 4 cut(s) 26, 194, 343, 1732
DdeI CTNAG 7 cut(s) 9, 487, 594, 894, 994, 1215, 1518
DpnI GATC 6 cut(s) 36, 216, 662, 1456, 1590, 1665
DpnII GATC 6 cut(s) 34, 214, 660, 1454, 1588, 1663
DraI TTTAAA 1 cut(s) 253
DraIII CACNNNGTG 1 cut(s) 959
Eam1104I CTCTTC 1 cut(s) 1075
EarI CTCTTC 1 cut(s) 1075
Eco130I CCWWGG 3 cut(s) 284, 664, 1296
Eco147I AGGCCT 1 cut(s) 1295
Eco31I GGTCTC 1 cut(s) 1521
Eco32I GATATC 1 cut(s) 922
Eco47I GGWCC 2 cut(s) 712, 947
Eco57I CTGAAG 4 cut(s) 362, 564, 1032, 1628
EcoO109I RGGNCCY 1 cut(s) 616
EcoRI GAATTC 1 cut(s) 742
EcoRII CCWGG 1 cut(s) 183
EcoRV GATATC 1 cut(s) 922
EcoT14I CCWWGG 3 cut(s) 284, 664, 1296
EcoT22I ATGCAT 1 cut(s) 541
ErhI CCWWGG 3 cut(s) 284, 664, 1296
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 8 cut(s) 243, 296, 578, 608, 677, 694, 959, 1106
FaqI GGGAC 1 cut(s) 802
FatI CATG 8 cut(s) 239, 292, 574, 604, 673, 690, 955, 1102
FbaI TGATCA 1 cut(s) 214
FblI GTMKAC 1 cut(s) 1184
Fnu4HI GCNGC 5 cut(s) 549, 768, 1158, 1161, 1674
FokI GGATG 2 cut(s) 203, 638
Fsp4HI GCNGC 5 cut(s) 549, 768, 1158, 1161, 1674
FspBI CTAG 6 cut(s) 14, 498, 1125, 1307, 1463, 1754
GlaI GCGC 2 cut(s) 562, 1523
GluI GCNGC 5 cut(s) 549, 768, 1158, 1161, 1674
GsuI CTGGAG 2 cut(s) 426, 732
HaeIII GGCC 4 cut(s) 307, 617, 1295, 1415
HgaI GACGC 3 cut(s) 298, 922, 1648
HhaI GCGC 2 cut(s) 563, 1524
Hin1II CATG 8 cut(s) 243, 296, 578, 608, 677, 694, 959, 1106
Hin6I GCGC 2 cut(s) 561, 1522
HinP1I GCGC 2 cut(s) 561, 1522
HincII GTYRAC 3 cut(s) 147, 1185, 1684
HindII GTYRAC 3 cut(s) 147, 1185, 1684
HindIII AAGCTT 1 cut(s) 1532
HinfI GANTC 5 cut(s) 54, 107, 491, 1385, 1508
HphI GGTGA 1 cut(s) 971
Hpy166II GTNNAC 5 cut(s) 147, 481, 1185, 1394, 1684
Hpy8I GTNNAC 5 cut(s) 147, 481, 1185, 1394, 1684
Hpy99I CGWCG 1 cut(s) 1671
HpyAV CCTTC 5 cut(s) 386, 466, 638, 935, 1475
HpyCH4III ACNGT 7 cut(s) 70, 151, 701, 793, 857, 871, 1688
HpyCH4IV ACGT 1 cut(s) 345
HpyF10VI GCNNNNNNNGC 1 cut(s) 545
HpyF3I CTNAG 7 cut(s) 9, 487, 594, 894, 994, 1215, 1518
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 8 cut(s) 243, 296, 578, 608, 677, 694, 959, 1106
HspAI GCGC 2 cut(s) 561, 1522
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 6 cut(s) 34, 214, 660, 1454, 1588, 1663
LmnI GCTCC 5 cut(s) 154, 267, 445, 724, 930
Lsp1109I GCAGC 5 cut(s) 560, 754, 1144, 1172, 1685
LweI GCATC 4 cut(s) 548, 853, 899, 994
MaeI CTAG 6 cut(s) 14, 498, 1125, 1307, 1463, 1754
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 7 cut(s) 64, 166, 235, 736, 817, 959, 1309
MalI GATC 6 cut(s) 36, 216, 662, 1456, 1590, 1665
MboI GATC 6 cut(s) 34, 214, 660, 1454, 1588, 1663
MboII GAAGA 8 cut(s) 29, 92, 217, 767, 1062, 1568, 1571, 1771
MfeI CAATTG 1 cut(s) 1626
MhlI GDGCHC 2 cut(s) 442, 1396
MlyI GAGTC 2 cut(s) 48, 500
MmeI TCCRAC 1 cut(s) 289
Mph1103I ATGCAT 1 cut(s) 541
MroXI GAANNNNTTC 2 cut(s) 589, 1145
MslI CAYNNNNRTG 2 cut(s) 579, 968
MspA1I CMGCKG 1 cut(s) 1250
MspCI CTTAAG 3 cut(s) 269, 1360, 1529
MspR9I CCNGG 1 cut(s) 185
MunI CAATTG 1 cut(s) 1626
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 1 cut(s) 185
MwoI GCNNNNNNNGC 1 cut(s) 545
NdeII GATC 6 cut(s) 34, 214, 660, 1454, 1588, 1663
NlaIII CATG 8 cut(s) 243, 296, 578, 608, 677, 694, 959, 1106
NlaIV GGNNCC 2 cut(s) 714, 832
NmuCI GTSAC 3 cut(s) 166, 736, 959
NsiI ATGCAT 1 cut(s) 541
NspI RCATGY 2 cut(s) 243, 296
OliI CACNNNNGTG 1 cut(s) 968
PaeI GCATGC 1 cut(s) 296
PceI AGGCCT 1 cut(s) 1295
PciI ACATGT 1 cut(s) 239
PctI GAATGC 1 cut(s) 541
PdmI GAANNNNTTC 2 cut(s) 589, 1145
PfeI GAWTC 3 cut(s) 107, 1385, 1508
Pfl23II CGTACG 1 cut(s) 342
PflMI CCANNNNNTGG 1 cut(s) 956
PkrI GCNGC 5 cut(s) 550, 769, 1159, 1162, 1675
PleI GAGTC 2 cut(s) 48, 499
PpsI GAGTC 2 cut(s) 48, 499
Ppu21I YACGTR 1 cut(s) 346
PscI ACATGT 1 cut(s) 239
PshBI ATTAAT 1 cut(s) 206
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspLI CGTACG 1 cut(s) 342
PspN4I GGNNCC 2 cut(s) 714, 832
PspPI GGNCC 4 cut(s) 306, 616, 712, 947
PstI CTGCAG 1 cut(s) 1162
PstNI CAGNNNCTG 1 cut(s) 494
RsaI GTAC 4 cut(s) 27, 195, 344, 1733
RsaNI GTAC 4 cut(s) 26, 194, 343, 1732
RseI CAYNNNNRTG 2 cut(s) 579, 968
SalI GTCGAC 1 cut(s) 1183
SatI GCNGC 5 cut(s) 549, 768, 1158, 1161, 1674
Sau3AI GATC 6 cut(s) 34, 214, 660, 1454, 1588, 1663
Sau96I GGNCC 4 cut(s) 306, 616, 712, 947
SchI GAGTC 2 cut(s) 48, 500
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 2 cut(s) 442, 1396
SfaNI GCATC 4 cut(s) 548, 853, 899, 994
SfcI CTRYAG 3 cut(s) 137, 1158, 1401
SinI GGWCC 2 cut(s) 712, 947
SmiMI CAYNNNNRTG 2 cut(s) 579, 968
SmlI CTYRAG 4 cut(s) 269, 1197, 1360, 1529
SmoI CTYRAG 4 cut(s) 269, 1197, 1360, 1529
SphI GCATGC 1 cut(s) 296
SseBI AGGCCT 1 cut(s) 1295
SsiI CCGC 2 cut(s) 429, 1250
SspI AATATT 1 cut(s) 97
SspMI CTAG 6 cut(s) 14, 498, 1125, 1307, 1463, 1754
StuI AGGCCT 1 cut(s) 1295
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 3 cut(s) 284, 664, 1296
TaaI ACNGT 7 cut(s) 70, 151, 701, 793, 857, 871, 1688
TaiI ACGT 1 cut(s) 348
TaqI TCGA 4 cut(s) 73, 533, 1149, 1184
TatI WGTACW 3 cut(s) 25, 193, 1731
TfiI GAWTC 3 cut(s) 107, 1385, 1508
TscAI CASTG 3 cut(s) 156, 440, 1401
TseFI GTSAC 3 cut(s) 166, 736, 959
TseI GCWGC 5 cut(s) 548, 767, 1157, 1160, 1673
Tsp45I GTSAC 3 cut(s) 166, 736, 959
TspDTI ATGAA 3 cut(s) 707, 1494, 1757
TspGWI ACGGA 1 cut(s) 185
TspRI CASTG 3 cut(s) 156, 440, 1401
Van91I CCANNNNNTGG 1 cut(s) 956
Vha464I CTTAAG 3 cut(s) 269, 1360, 1529
VneI GTGCAC 1 cut(s) 1392
VpaK11BI GGWCC 2 cut(s) 712, 947
VspI ATTAAT 1 cut(s) 206
XapI RAATTY 5 cut(s) 248, 351, 365, 742, 754
XbaI TCTAGA 1 cut(s) 1753
XceI RCATGY 2 cut(s) 243, 296
XmiI GTMKAC 1 cut(s) 1184
XmnI GAANNNNTTC 2 cut(s) 589, 1145
XspI CTAG 6 cut(s) 14, 498, 1125, 1307, 1463, 1754
Zsp2I ATGCAT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.