Rw6G002260

Iron-sulfur assembly protein IscA-like 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0030407
Physical Location & Seq
Reverse (-)
3770065 .. 3771825
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002260.1

Sequence Viewer

Length: 312 bp
ATGGCCTCGCAGATTCTGGCGGCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGATGGCCCGGGCAGCTTCTAGAATACGGCAGCTGCTAGAGCAGCGCCAGCGGTCTTATCTGAAGCTCGAGGTTAAGGAGCGTGGCTGCAACGGCTTGTCGTACACTCTCAATTATGCAGATGATAGACAGTTCGATGAGTTGGCTCTGATGCATGTTATTGGAACCAAGATGGATTTTGTGGATGACAAACTCAGGTCTGCTTTTATATTTATCAATCCCAAATCAAATGGACAATGCGGCTGCATGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fe-S_biosyn

Protein Analysis

103

Amino Acids

11.23

Weight (kDa)

9.22

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030407)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0245041
rosa_wichuraiana Rw6G002260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 20, 109, 297
AcoI YGGCCR 1 cut(s) 21
AcuI CTGAAG 1 cut(s) 140
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 1 cut(s) 30
AluBI AGCT 3 cut(s) 74, 91, 124
AluI AGCT 3 cut(s) 74, 91, 124
AlwNI CAGNNNCTG 1 cut(s) 16
Ama87I CYCGRG 2 cut(s) 66, 125
AoxI GGCC 3 cut(s) 3, 21, 63
ApeKI GCWGC 6 cut(s) 71, 88, 91, 100, 144, 300
AspLEI GCGC 1 cut(s) 105
AspS9I GGNCC 1 cut(s) 64
AsuC2I CCSGG 3 cut(s) 25, 67, 68
AvaI CYCGRG 2 cut(s) 66, 125
BbvI GCAGC 6 cut(s) 78, 83, 100, 112, 131, 287
BccI CCATC 2 cut(s) 55, 223
BceAI ACGGC 2 cut(s) 101, 166
BcnI CCSGG 3 cut(s) 25, 67, 68
BfaI CTAG 2 cut(s) 78, 95
BfoI RGCGCY 1 cut(s) 106
BisI GCNGC 8 cut(s) 21, 72, 89, 92, 101, 145, 298, 301
BlsI GCNGC 8 cut(s) 22, 73, 90, 93, 102, 146, 299, 302
Bme1390I CCNGG 3 cut(s) 25, 67, 68
BmeT110I CYCGRG 2 cut(s) 66, 125
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 1 cut(s) 223
BmrFI CCNGG 3 cut(s) 25, 67, 68
BmsI GCATC 1 cut(s) 198
BpuMI CCSGG 3 cut(s) 25, 67, 68
BsaJI CCNNGG 2 cut(s) 24, 66
Bsc4I CCNNNNNNNGG 1 cut(s) 30
BseDI CCNNGG 2 cut(s) 24, 66
BseGI GGATG 2 cut(s) 66, 247
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMII CTCAG 1 cut(s) 265
BseX3I CGGCCG 1 cut(s) 21
BseXI GCAGC 6 cut(s) 78, 83, 100, 112, 131, 287
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 3 cut(s) 5, 23, 65
BsiEI CGRYCG 1 cut(s) 24
BsiHKCI CYCGRG 2 cut(s) 66, 125
BsiSI CCGG 2 cut(s) 24, 67
BslI CCNNNNNNNGG 1 cut(s) 30
BsnI GGCC 3 cut(s) 5, 23, 65
BsoBI CYCGRG 2 cut(s) 66, 125
BspACI CCGC 3 cut(s) 20, 109, 297
BspANI GGCC 3 cut(s) 5, 23, 65
BspCNI CTCAG 1 cut(s) 264
BspLI GGNNCC 1 cut(s) 223
BssECI CCNNGG 2 cut(s) 24, 66
Bst4CI ACNGT 1 cut(s) 189
BstC8I GCNNGC 1 cut(s) 107
BstDEI CTNAG 1 cut(s) 251
BstF5I GGATG 2 cut(s) 66, 247
BstH2I RGCGCY 1 cut(s) 106
BstHHI GCGC 1 cut(s) 105
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 5 cut(s) 71, 97, 100, 106, 150
BstNSI RCATGY 2 cut(s) 215, 307
BstSCI CCNGG 3 cut(s) 23, 65, 66
BstV1I GCAGC 6 cut(s) 78, 83, 100, 112, 131, 287
BstZI CGGCCG 1 cut(s) 21
BsuRI GGCC 3 cut(s) 5, 23, 65
BtsCI GGATG 2 cut(s) 66, 247
Cac8I GCNNGC 1 cut(s) 107
CaiI CAGNNNCTG 1 cut(s) 16
CfoI GCGC 1 cut(s) 105
Cfr13I GGNCC 1 cut(s) 64
Cfr9I CCCGGG 1 cut(s) 66
Csp6I GTAC 1 cut(s) 160
CviAII CATG 2 cut(s) 212, 304
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 251
EaeI YGGCCR 1 cut(s) 21
EagI CGGCCG 1 cut(s) 21
EclXI CGGCCG 1 cut(s) 21
Eco52I CGGCCG 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 140
Eco88I CYCGRG 2 cut(s) 66, 125
EcoT22I ATGCAT 1 cut(s) 213
FaeI CATG 2 cut(s) 215, 307
FaiI YATR 4 cut(s) 174, 213, 266, 305
FatI CATG 2 cut(s) 211, 303
Fnu4HI GCNGC 8 cut(s) 21, 72, 89, 92, 101, 145, 298, 301
FokI GGATG 2 cut(s) 73, 254
Fsp4HI GCNGC 8 cut(s) 21, 72, 89, 92, 101, 145, 298, 301
FspBI CTAG 2 cut(s) 78, 95
GlaI GCGC 1 cut(s) 104
GluI GCNGC 8 cut(s) 21, 72, 89, 92, 101, 145, 298, 301
HaeII RGCGCY 1 cut(s) 106
HaeIII GGCC 3 cut(s) 5, 23, 65
HapII CCGG 2 cut(s) 24, 67
HhaI GCGC 1 cut(s) 105
Hin1II CATG 2 cut(s) 215, 307
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HinfI GANTC 1 cut(s) 13
HpaII CCGG 2 cut(s) 24, 67
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 2 cut(s) 120, 207
Hpy188III TCNNGA 1 cut(s) 78
Hpy8I GTNNAC 1 cut(s) 162
HpyCH4III ACNGT 1 cut(s) 189
HpyCH4V TGCA 4 cut(s) 147, 176, 211, 303
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 97, 100, 106, 150
HpyF3I CTNAG 1 cut(s) 251
Hsp92II CATG 2 cut(s) 215, 307
HspAI GCGC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 136
LpnPI CCDG 5 cut(s) 2, 37, 80, 119, 238
Lsp1109I GCAGC 6 cut(s) 78, 83, 100, 112, 131, 287
LweI GCATC 1 cut(s) 198
MaeI CTAG 2 cut(s) 78, 95
MluCI AATT 1 cut(s) 169
MnlI CCTC 3 cut(s) 16, 51, 121
Mph1103I ATGCAT 1 cut(s) 213
MseI TTAA 1 cut(s) 132
MspA1I CMGCKG 2 cut(s) 91, 109
MspI CCGG 2 cut(s) 24, 67
MspR9I CCNGG 3 cut(s) 25, 67, 68
MwoI GCNNNNNNNGC 5 cut(s) 71, 97, 100, 106, 150
NciI CCSGG 3 cut(s) 25, 67, 68
NlaIII CATG 2 cut(s) 215, 307
NlaIV GGNNCC 1 cut(s) 223
NsiI ATGCAT 1 cut(s) 213
NspI RCATGY 2 cut(s) 215, 307
PaeR7I CTCGAG 1 cut(s) 125
PfeI GAWTC 1 cut(s) 13
PkrI GCNGC 8 cut(s) 22, 73, 90, 93, 102, 146, 299, 302
PspN4I GGNNCC 1 cut(s) 223
PspPI GGNCC 1 cut(s) 64
PspXI VCTCGAGB 1 cut(s) 125
PstNI CAGNNNCTG 1 cut(s) 16
PvuII CAGCTG 1 cut(s) 91
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 8 cut(s) 21, 72, 89, 92, 101, 145, 298, 301
Sau96I GGNCC 1 cut(s) 64
ScrFI CCNGG 3 cut(s) 25, 67, 68
SetI ASST 5 cut(s) 76, 93, 126, 132, 257
SfaNI GCATC 1 cut(s) 198
Sfr274I CTCGAG 1 cut(s) 125
SlaI CTCGAG 1 cut(s) 125
SmaI CCCGGG 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SrfI GCCCGGGC 1 cut(s) 68
Sse9I AATT 1 cut(s) 169
SsiI CCGC 3 cut(s) 20, 109, 297
SspMI CTAG 2 cut(s) 78, 95
StyD4I CCNGG 3 cut(s) 23, 65, 66
TaaI ACNGT 1 cut(s) 189
TaqI TCGA 2 cut(s) 126, 192
TasI AATT 1 cut(s) 169
TauI GCSGC 2 cut(s) 23, 300
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TseI GCWGC 6 cut(s) 71, 88, 91, 100, 144, 300
TspMI CCCGGG 1 cut(s) 66
XbaI TCTAGA 1 cut(s) 77
XceI RCATGY 2 cut(s) 215, 307
XhoI CTCGAG 1 cut(s) 125
XmaI CCCGGG 1 cut(s) 66
XspI CTAG 2 cut(s) 78, 95
Zsp2I ATGCAT 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.