Rw6G036690

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0017252
Physical Location & Seq
Forward (+)
61121514 .. 61123058
1545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G036690.1

Sequence Viewer

Length: 1410 bp
ATGGTTTTGTTGACGGTGTCAGCCTCCGTGGATAGCTTACGTCCCCCTCCATGCACGGCAAGACCATGCAACAAAGCAACCGATGGCCAAACATGGTTCCTATACGCTGCACTCGGCCTCATAGCCCTCGGAACAGGTGGCATCAAACCATGTGTCTCGTCTTTTGGAGCTGATCAATTCGATGAGGCTGATACAAATGAAGTCCAAAAGAAATACTCATTCTTCAACTGGTTCTTCTTTGGCATAAACATGGGTGCACTCTTGGGCATTACACTACTCGTTTACCTACAAGAAAATAAGGGTTGGACTTGGGGCTTCGGAGTTCCAACAGTTGCCATGGTTTGCTCGATTGTAATCTTGGCTGCCGGAATTCCTAAATATCGTTACCAAAAGCCAATGGGAAGCCCCTTTACTAGGTTTATTCAGGTCATCGTTGTCTCACTCAGGAACCATTCTCGACGTGTTGAAGTGGGAAGTGAAGCAGCGTTATACGAGGTCAACACCGAGGAGTCTGATATTGTGGGGGCGAGAAAGCTACCTCATACTGCACAATACAGATTTCTGGACAAAGCAGCACTTGTGACAGACCTAGAGGACAACCCAAAGACTCGTTGGAGGCTATGCACAGTGACCCAAGTTGAAGAATTTAAATGCTTCATTAGGGTCCTTCCCATATGGGCATCAACCATAGCTCTCTCCCTCTCCTTCGCTCAACTTTCAACCTTCTTCATAAGCCAAGCTGCCCTCATGGACCGCAAGCTTGGTCCCGACTTCGAAATCCCTGCAGGCTCTGTCCCCGTCTTCAGCGCCGTCAACGCCCTCATTCTCGTCCCTATCTATGAAAAATGGATGGTTCCGATCATCAGTAGGAGGACCGGCCACCACCGTGGTATCACATCGTTGCAAAGAATGGGTGTCGGACTCTTTATCTCCATCTTTGCTCTGGCTTCAGCCGCTTTGGTAGAGAAGAAACGCCGAGACCACTACTCGAAACCGTTTAGCATGAGTGTGTTTTGGTTGCTTCCACAATTCTTTCTGATTGGTGCCGCCGAGGTTTTTACTTACGTTGGACAGTTGGAGTTTTTCTACGATGAAGCTACTGATGGTACGAGAAGTATAAGTAGTGCAATGTTCCTGAGTGAGATAGGAATTGGGAGCTGGTTAAGCACTGCGCTTGTTAAGATCATAGAGGGTACAACTGGTGGAGAAACAAAAGGGTGGTTGAGGGCTACTCTTAATAAGAGCAAGCTTGATTATTTCTATTGGATATTGACAGCCATTAATGGTCTGAATTTCCTTGTGTATCTGTGGGTTTCACGGCGTTATAAAGGTAGACACGGAACCAGCACAAGTGTGAGAGACGGCCGGGAAGAGTGGAGAACTGATCATGAATTTGAAGTATGGGCGTGA

Protein Analysis

469

Amino Acids

52.62

Weight (kDa)

8.97

Isoelectric Point (pI)

32.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 1 - 407 1.1e-97 POT family
MFS_1 PF07690 30 - 378 9.4e-10 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017252)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g38910
malus_domestica MD08G1022500.v1.1 MD15G1019900.v1.1
prunus_persica Prupe.1G371900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0302001
rosa_laevigata RLG00000011204
rosa_multiflora Rmu_sc0006892.1_g000005
rosa_roxburghii Rroxscaffold_7G00166200
rosa_rugosa Rorug06G0311000
rosa_samantha Rh6BG430200 Rh6CG436600 Rh6DG423600
rosa_wichuraiana Rw6G036690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1326
AccB1I GGYRCC 1 cut(s) 1043
AccI GTMKAC 1 cut(s) 1333
AciI CCGC 3 cut(s) 754, 954, 1047
AcoI YGGCCR 3 cut(s) 85, 877, 1363
AcsI RAATTY 4 cut(s) 369, 644, 1291, 1391
AcuI CTGAAG 2 cut(s) 787, 933
AfaI GTAC 2 cut(s) 1108, 1195
AfiI CCNNNNNNNGG 1 cut(s) 414
AflIII ACRYGT 1 cut(s) 460
AgsI TTSAA 5 cut(s) 226, 467, 641, 720, 1397
AjiI CACGTC 1 cut(s) 461
AleI CACNNNNGTG 2 cut(s) 885, 1352
AluBI AGCT 9 cut(s) 36, 170, 535, 692, 740, 760, 1097, 1158, 1249
AluI AGCT 9 cut(s) 36, 170, 535, 692, 740, 760, 1097, 1158, 1249
Alw21I GWGCWC 1 cut(s) 259
Alw26I GTCTC 4 cut(s) 160, 442, 972, 1353
Alw44I GTGCAC 1 cut(s) 255
AlwNI CAGNNNCTG 1 cut(s) 791
AoxI GGCC 4 cut(s) 85, 115, 877, 1363
ApaLI GTGCAC 1 cut(s) 255
ApeKI GCWGC 5 cut(s) 107, 362, 482, 572, 740
ApoI RAATTY 4 cut(s) 369, 644, 1291, 1391
ArsI GACNNNNNNTTYG 2 cut(s) 138, 170
AseI ATTAAT 1 cut(s) 1281
AspLEI GCGC 2 cut(s) 809, 1174
AspS9I GGNCC 4 cut(s) 664, 751, 764, 873
AsuC2I CCSGG 1 cut(s) 1367
AsuII TTCGAA 1 cut(s) 774
AvaII GGWCC 4 cut(s) 664, 751, 764, 873
BaeGI GKGCMC 1 cut(s) 259
BaeI ACNNNNGTAYC 2 cut(s) 874, 907
BalI TGGCCA 1 cut(s) 87
BanI GGYRCC 1 cut(s) 1043
BarI GAAGNNNNNNTAC 2 cut(s) 394, 426
BbsI GAAGAC 1 cut(s) 793
Bbv12I GWGCWC 1 cut(s) 259
BbvI GCAGC 5 cut(s) 94, 349, 494, 584, 727
BccI CCATC 4 cut(s) 77, 844, 941, 1097
BceAI ACGGC 4 cut(s) 72, 794, 1334, 1378
BcgI CGANNNNNNTGC 2 cut(s) 764, 798
BclI TGATCA 2 cut(s) 172, 1384
BcnI CCSGG 1 cut(s) 1367
BcoDI GTCTC 4 cut(s) 160, 442, 972, 1353
BfaI CTAG 2 cut(s) 414, 590
BfmI CTRYAG 1 cut(s) 783
BfoI RGCGCY 1 cut(s) 810
BisI GCNGC 7 cut(s) 108, 363, 483, 573, 741, 954, 1047
BlsI GCNGC 7 cut(s) 109, 364, 484, 574, 742, 955, 1048
Bme1390I CCNGG 1 cut(s) 1367
Bme18I GGWCC 4 cut(s) 664, 751, 764, 873
BmgBI CACGTC 1 cut(s) 461
BmgT120I GGNCC 4 cut(s) 664, 751, 764, 873
BmiI GGNNCC 7 cut(s) 98, 449, 665, 766, 855, 1045, 1342
BmrFI CCNGG 1 cut(s) 1367
BmsI GCATC 2 cut(s) 150, 689
BpiI GAAGAC 1 cut(s) 793
BplI GAGNNNNNCTC 2 cut(s) 1216, 1248
Bpu14I TTCGAA 1 cut(s) 774
BpuMI CCSGG 1 cut(s) 1367
BsaBI GATNNNNATC 1 cut(s) 353
BsaI GGTCTC 1 cut(s) 972
BsaJI CCNNGG 6 cut(s) 27, 127, 336, 504, 886, 1050
Bsc4I CCNNNNNNNGG 1 cut(s) 414
Bse118I RCCGGY 1 cut(s) 875
Bse1I ACTGG 2 cut(s) 233, 1204
Bse3DI GCAATG 1 cut(s) 1134
Bse8I GATNNNNATC 1 cut(s) 353
BseDI CCNNGG 6 cut(s) 27, 127, 336, 504, 886, 1050
BseGI GGATG 1 cut(s) 855
BseJI GATNNNNATC 1 cut(s) 353
BseLI CCNNNNNNNGG 1 cut(s) 414
BseMI GCAATG 1 cut(s) 1134
BseMII CTCAG 2 cut(s) 457, 1127
BseNI ACTGG 2 cut(s) 233, 1204
BseRI GAGGAG 1 cut(s) 521
BseSI GKGCMC 1 cut(s) 259
BseX3I CGGCCG 1 cut(s) 1363
BseXI GCAGC 5 cut(s) 94, 349, 494, 584, 727
BsgI GTGCAG 2 cut(s) 93, 531
Bsh1285I CGRYCG 1 cut(s) 1366
BshFI GGCC 4 cut(s) 87, 117, 879, 1365
BshNI GGYRCC 1 cut(s) 1043
BsiEI CGRYCG 1 cut(s) 1366
BsiHKAI GWGCWC 1 cut(s) 259
BsiSI CCGG 3 cut(s) 366, 876, 1366
BslFI GGGAC 4 cut(s) 27, 750, 779, 815
BslI CCNNNNNNNGG 1 cut(s) 414
BsmAI GTCTC 4 cut(s) 160, 442, 972, 1353
BsmBI CGTCTC 1 cut(s) 1353
BsmFI GGGAC 4 cut(s) 27, 750, 779, 815
BsnI GGCC 4 cut(s) 87, 117, 879, 1365
Bso31I GGTCTC 1 cut(s) 972
Bsp119I TTCGAA 1 cut(s) 774
Bsp1286I GDGCHC 1 cut(s) 259
Bsp143I GATC 4 cut(s) 172, 858, 1182, 1384
Bsp19I CCATGG 1 cut(s) 336
BspACI CCGC 3 cut(s) 754, 954, 1047
BspANI GGCC 4 cut(s) 87, 117, 879, 1365
BspCNI CTCAG 2 cut(s) 456, 1128
BspHI TCATGA 1 cut(s) 1387
BspLI GGNNCC 7 cut(s) 98, 449, 665, 766, 855, 1045, 1342
BspMAI CTGCAG 1 cut(s) 787
BspT104I TTCGAA 1 cut(s) 774
BspT107I GGYRCC 1 cut(s) 1043
BspTNI GGTCTC 1 cut(s) 972
BsrDI GCAATG 1 cut(s) 1134
BsrFI RCCGGY 1 cut(s) 875
BsrI ACTGG 2 cut(s) 233, 1204
BssAI RCCGGY 1 cut(s) 875
BssECI CCNNGG 6 cut(s) 27, 127, 336, 504, 886, 1050
BssMI GATC 4 cut(s) 172, 858, 1182, 1384
BssT1I CCWWGG 1 cut(s) 336
Bst4CI ACNGT 6 cut(s) 16, 331, 628, 887, 996, 1074
Bst6I CTCTTC 1 cut(s) 1365
BstBI TTCGAA 1 cut(s) 774
BstC8I GCNNGC 3 cut(s) 758, 787, 1247
BstDEI CTNAG 2 cut(s) 443, 1136
BstDSI CCRYGG 3 cut(s) 27, 336, 886
BstENI CCTNNNNNAGG 1 cut(s) 412
BstF5I GGATG 1 cut(s) 855
BstH2I RGCGCY 1 cut(s) 810
BstHHI GCGC 2 cut(s) 809, 1174
BstKTI GATC 4 cut(s) 175, 861, 1185, 1387
BstMAI GTCTC 4 cut(s) 160, 442, 972, 1353
BstMBI GATC 4 cut(s) 172, 858, 1182, 1384
BstMCI CGRYCG 1 cut(s) 1366
BstMWI GCNNNNNNNGC 3 cut(s) 815, 953, 1164
BstSCI CCNGG 1 cut(s) 1365
BstSFI CTRYAG 1 cut(s) 783
BstSLI GKGCMC 1 cut(s) 259
BstV1I GCAGC 5 cut(s) 94, 349, 494, 584, 727
BstV2I GAAGAC 1 cut(s) 793
BstXI CCANNNNNNTGG 1 cut(s) 887
BstZI CGGCCG 1 cut(s) 1363
BsuRI GGCC 4 cut(s) 87, 117, 879, 1365
BtgI CCRYGG 3 cut(s) 27, 336, 886
BtrI CACGTC 1 cut(s) 461
BtsCI GGATG 1 cut(s) 855
BtsI GCAGTG 1 cut(s) 1167
BtsIMutI CAGTG 2 cut(s) 633, 1167
Cac8I GCNNGC 3 cut(s) 758, 787, 1247
CaiI CAGNNNCTG 1 cut(s) 791
CciI TCATGA 1 cut(s) 1387
CfoI GCGC 2 cut(s) 809, 1174
Cfr10I RCCGGY 1 cut(s) 875
Cfr13I GGNCC 4 cut(s) 664, 751, 764, 873
Csp6I GTAC 2 cut(s) 1107, 1194
CviAII CATG 9 cut(s) 51, 66, 93, 150, 250, 337, 748, 1003, 1388
CviQI GTAC 2 cut(s) 1107, 1194
DdeI CTNAG 2 cut(s) 443, 1136
DpnI GATC 4 cut(s) 174, 860, 1184, 1386
DpnII GATC 4 cut(s) 172, 858, 1182, 1384
DraI TTTAAA 1 cut(s) 649
EaeI YGGCCR 3 cut(s) 85, 877, 1363
EagI CGGCCG 1 cut(s) 1363
Eam1104I CTCTTC 1 cut(s) 1365
EarI CTCTTC 1 cut(s) 1365
EclXI CGGCCG 1 cut(s) 1363
Eco130I CCWWGG 1 cut(s) 336
Eco31I GGTCTC 1 cut(s) 972
Eco47I GGWCC 4 cut(s) 664, 751, 764, 873
Eco52I CGGCCG 1 cut(s) 1363
Eco57I CTGAAG 2 cut(s) 787, 933
EcoNI CCTNNNNNAGG 1 cut(s) 412
EcoO109I RGGNCCY 1 cut(s) 664
EcoRI GAATTC 1 cut(s) 369
EcoT14I CCWWGG 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 336
Esp3I CGTCTC 1 cut(s) 1353
FaeI CATG 9 cut(s) 54, 69, 96, 153, 253, 340, 751, 1006, 1391
FalI AAGNNNNNCTT 2 cut(s) 561, 593
FaqI GGGAC 4 cut(s) 27, 750, 779, 815
FatI CATG 9 cut(s) 50, 65, 92, 149, 249, 336, 747, 1002, 1387
FauNDI CATATG 1 cut(s) 674
FbaI TGATCA 2 cut(s) 172, 1384
FblI GTMKAC 1 cut(s) 1333
Fnu4HI GCNGC 7 cut(s) 108, 363, 483, 573, 741, 954, 1047
FokI GGATG 1 cut(s) 862
Fsp4HI GCNGC 7 cut(s) 108, 363, 483, 573, 741, 954, 1047
FspBI CTAG 2 cut(s) 414, 590
GlaI GCGC 2 cut(s) 808, 1173
GluI GCNGC 7 cut(s) 108, 363, 483, 573, 741, 954, 1047
HaeII RGCGCY 1 cut(s) 810
HaeIII GGCC 4 cut(s) 87, 117, 879, 1365
HapII CCGG 3 cut(s) 366, 876, 1366
HhaI GCGC 2 cut(s) 809, 1174
Hin1II CATG 9 cut(s) 54, 69, 96, 153, 253, 340, 751, 1006, 1391
Hin6I GCGC 2 cut(s) 807, 1172
HinP1I GCGC 2 cut(s) 807, 1172
HincII GTYRAC 3 cut(s) 12, 499, 814
HindII GTYRAC 3 cut(s) 12, 499, 814
HindIII AAGCTT 2 cut(s) 758, 1247
HinfI GANTC 3 cut(s) 509, 607, 921
HpaII CCGG 3 cut(s) 366, 876, 1366
Hpy166II GTNNAC 6 cut(s) 12, 257, 283, 499, 814, 1334
Hpy188I TCNGA 7 cut(s) 131, 320, 514, 858, 920, 1038, 1290
Hpy188III TCNNGA 6 cut(s) 445, 456, 563, 767, 1135, 1388
Hpy8I GTNNAC 6 cut(s) 12, 257, 283, 499, 814, 1334
Hpy99I CGWCG 1 cut(s) 462
HpyAV CCTTC 3 cut(s) 677, 715, 733
HpyCH4III ACNGT 6 cut(s) 16, 331, 628, 887, 996, 1074
HpyCH4IV ACGT 3 cut(s) 40, 460, 1065
HpyCH4V TGCA 9 cut(s) 54, 69, 110, 257, 548, 624, 785, 904, 1127
HpyF10VI GCNNNNNNNGC 3 cut(s) 815, 953, 1164
HpyF3I CTNAG 2 cut(s) 443, 1136
HpySE526I ACGT 3 cut(s) 40, 460, 1065
Hsp92II CATG 9 cut(s) 54, 69, 96, 153, 253, 340, 751, 1006, 1391
HspAI GCGC 2 cut(s) 807, 1172
Ksp22I TGATCA 2 cut(s) 172, 1384
Kzo9I GATC 4 cut(s) 172, 858, 1182, 1384
LmnI GCTCC 2 cut(s) 167, 1155
Lsp1109I GCAGC 5 cut(s) 94, 349, 494, 584, 727
LweI GCATC 2 cut(s) 150, 689
MaeI CTAG 2 cut(s) 414, 590
MaeII ACGT 3 cut(s) 40, 460, 1065
MaeIII GTNAC 3 cut(s) 383, 580, 628
MalI GATC 4 cut(s) 174, 860, 1184, 1386
MboI GATC 4 cut(s) 172, 858, 1182, 1384
MboII GAAGA 7 cut(s) 214, 226, 653, 718, 793, 979, 1382
MhlI GDGCHC 1 cut(s) 259
MlsI TGGCCA 1 cut(s) 87
MluCI AATT 7 cut(s) 176, 369, 644, 1028, 1149, 1291, 1391
MluNI TGGCCA 1 cut(s) 87
MlyI GAGTC 3 cut(s) 518, 601, 915
MmeI TCCRAC 6 cut(s) 284, 350, 593, 898, 1048, 1056
Mox20I TGGCCA 1 cut(s) 87
MscI TGGCCA 1 cut(s) 87
MseI TTAA 5 cut(s) 648, 1163, 1179, 1236, 1281
MslI CAYNNNNRTG 4 cut(s) 248, 885, 1007, 1352
Msp20I TGGCCA 1 cut(s) 87
MspI CCGG 3 cut(s) 366, 876, 1366
MspR9I CCNGG 1 cut(s) 1367
MwoI GCNNNNNNNGC 3 cut(s) 815, 953, 1164
NciI CCSGG 1 cut(s) 1367
NcoI CCATGG 1 cut(s) 336
NdeI CATATG 1 cut(s) 674
NdeII GATC 4 cut(s) 172, 858, 1182, 1384
NlaIII CATG 9 cut(s) 54, 69, 96, 153, 253, 340, 751, 1006, 1391
NlaIV GGNNCC 7 cut(s) 98, 449, 665, 766, 855, 1045, 1342
NmeAIII GCCGAG 3 cut(s) 93, 1001, 1075
NmuCI GTSAC 2 cut(s) 580, 628
NspV TTCGAA 1 cut(s) 774
OliI CACNNNNGTG 2 cut(s) 885, 1352
PagI TCATGA 1 cut(s) 1387
PflFI GACNNNGTC 1 cut(s) 16
PkrI GCNGC 7 cut(s) 109, 364, 484, 574, 742, 955, 1048
PleI GAGTC 3 cut(s) 517, 601, 915
PpsI GAGTC 3 cut(s) 517, 601, 915
PpuMI RGGWCCY 1 cut(s) 664
PshBI ATTAAT 1 cut(s) 1281
PsiI TTATAA 1 cut(s) 1326
Psp5II RGGWCCY 1 cut(s) 664
PspN4I GGNNCC 7 cut(s) 98, 449, 665, 766, 855, 1045, 1342
PspPI GGNCC 4 cut(s) 664, 751, 764, 873
PspPPI RGGWCCY 1 cut(s) 664
PstI CTGCAG 1 cut(s) 787
PstNI CAGNNNCTG 1 cut(s) 791
PsyI GACNNNGTC 1 cut(s) 16
RsaI GTAC 2 cut(s) 1108, 1195
RsaNI GTAC 2 cut(s) 1107, 1194
RseI CAYNNNNRTG 4 cut(s) 248, 885, 1007, 1352
SaqAI TTAA 5 cut(s) 648, 1163, 1179, 1236, 1281
SatI GCNGC 7 cut(s) 108, 363, 483, 573, 741, 954, 1047
Sau3AI GATC 4 cut(s) 172, 858, 1182, 1384
Sau96I GGNCC 4 cut(s) 664, 751, 764, 873
SbfI CCTGCAGG 1 cut(s) 787
SchI GAGTC 3 cut(s) 518, 601, 915
ScrFI CCNGG 1 cut(s) 1367
SdaI CCTGCAGG 1 cut(s) 787
SduI GDGCHC 1 cut(s) 259
SfaNI GCATC 2 cut(s) 150, 689
SfcI CTRYAG 1 cut(s) 783
SfuI TTCGAA 1 cut(s) 774
SinI GGWCC 4 cut(s) 664, 751, 764, 873
SmiI ATTTAAAT 1 cut(s) 649
SmiMI CAYNNNNRTG 4 cut(s) 248, 885, 1007, 1352
Sse8387I CCTGCAGG 1 cut(s) 787
Sse9I AATT 7 cut(s) 176, 369, 644, 1028, 1149, 1291, 1391
SsiI CCGC 3 cut(s) 754, 954, 1047
SspMI CTAG 2 cut(s) 414, 590
StyD4I CCNGG 1 cut(s) 1365
StyI CCWWGG 1 cut(s) 336
SwaI ATTTAAAT 1 cut(s) 649
TaaI ACNGT 6 cut(s) 16, 331, 628, 887, 996, 1074
TaiI ACGT 3 cut(s) 43, 463, 1068
TaqI TCGA 5 cut(s) 180, 347, 457, 774, 989
TasI AATT 7 cut(s) 176, 369, 644, 1028, 1149, 1291, 1391
TauI GCSGC 2 cut(s) 956, 1049
Tru1I TTAA 5 cut(s) 648, 1163, 1179, 1236, 1281
Tru9I TTAA 5 cut(s) 648, 1163, 1179, 1236, 1281
TscAI CASTG 2 cut(s) 633, 1174
TseFI GTSAC 2 cut(s) 580, 628
TseI GCWGC 5 cut(s) 107, 362, 482, 572, 740
Tsp45I GTSAC 2 cut(s) 580, 628
TspDTI ATGAA 6 cut(s) 213, 646, 718, 855, 1107, 1404
TspGWI ACGGA 2 cut(s) 16, 1353
TspRI CASTG 2 cut(s) 633, 1174
Tth111I GACNNNGTC 1 cut(s) 16
VneI GTGCAC 1 cut(s) 255
VpaK11BI GGWCC 4 cut(s) 664, 751, 764, 873
VspI ATTAAT 1 cut(s) 1281
XagI CCTNNNNNAGG 1 cut(s) 412
XapI RAATTY 4 cut(s) 369, 644, 1291, 1391
XcmI CCANNNNNNNNNTGG 2 cut(s) 609, 940
XmiI GTMKAC 1 cut(s) 1333
XspI CTAG 2 cut(s) 414, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.