Rw7G025000

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0019930
Physical Location & Seq
Forward (+)
31244846 .. 31245280
435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025000.1

Sequence Viewer

Length: 189 bp
ATGGTAGGAGATAATCCCTTAGTTGACATCAAGGGTGCACGACAGGCTGGCCATCCCTGGTTTTCCATTCTGACAAGGACTGGAGTTTTCAAGGGAAAGGAAAATCATGACGAGTTTCCAGCTGATCTGGTTGTTGATACTGTCGAAGAAGCTGTGGACTATGTTTTGAAAAAGGAGTCGATCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_6 Hydrolase_like

Protein Analysis

62

Amino Acids

6.87

Weight (kDa)

4.88

Isoelectric Point (pI)

4.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_like PF13242 1 - 51 2.1e-09 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019930)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0046071
rosa_roxburghii Rroxscaffold_5G00349070
rosa_rugosa Rorug06G0047900
rosa_samantha Rh5BG315100 Rh5DG325300
rosa_wichuraiana Rw2G027100 Rw7G025000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 49
AgsI TTSAA 2 cut(s) 91, 169
AjnI CCWGG 1 cut(s) 56
AluBI AGCT 2 cut(s) 122, 152
AluI AGCT 2 cut(s) 122, 152
Alw21I GWGCWC 1 cut(s) 40
Alw44I GTGCAC 1 cut(s) 36
AoxI GGCC 1 cut(s) 49
ApaLI GTGCAC 1 cut(s) 36
BaeGI GKGCMC 1 cut(s) 40
BalI TGGCCA 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 40
BccI CCATC 1 cut(s) 60
BciT130I CCWGG 1 cut(s) 58
Bme1390I CCNGG 1 cut(s) 58
BmrFI CCNGG 1 cut(s) 58
BplI GAGNNNNNCTC 1 cut(s) 167
BpmI CTGGAG 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 85
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 1 cut(s) 56
BseGI GGATG 1 cut(s) 52
BseNI ACTGG 1 cut(s) 85
BseSI GKGCMC 1 cut(s) 40
BshFI GGCC 1 cut(s) 51
BsiHKAI GWGCWC 1 cut(s) 40
BsnI GGCC 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 40
Bsp143I GATC 2 cut(s) 124, 180
BspANI GGCC 1 cut(s) 51
BspHI TCATGA 1 cut(s) 106
BsrI ACTGG 1 cut(s) 85
BssECI CCNNGG 1 cut(s) 56
BssMI GATC 2 cut(s) 124, 180
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 1 cut(s) 142
BstC8I GCNNGC 1 cut(s) 49
BstDEI CTNAG 2 cut(s) 19, 186
BstF5I GGATG 1 cut(s) 52
BstKTI GATC 2 cut(s) 127, 183
BstMBI GATC 2 cut(s) 124, 180
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNI CCWGG 1 cut(s) 58
BstSCI CCNGG 1 cut(s) 56
BstSLI GKGCMC 1 cut(s) 40
BsuRI GGCC 1 cut(s) 51
BtsCI GGATG 1 cut(s) 52
Cac8I GCNNGC 1 cut(s) 49
CciI TCATGA 1 cut(s) 106
CviAII CATG 1 cut(s) 107
CviJI RGCY 4 cut(s) 47, 51, 122, 152
CviKI_1 RGCY 4 cut(s) 47, 51, 122, 152
DdeI CTNAG 2 cut(s) 19, 186
DpnI GATC 2 cut(s) 126, 182
DpnII GATC 2 cut(s) 124, 180
EaeI YGGCCR 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 56
FaeI CATG 1 cut(s) 110
FaiI YATR 2 cut(s) 108, 162
FatI CATG 1 cut(s) 106
FokI GGATG 1 cut(s) 39
GsuI CTGGAG 1 cut(s) 102
HaeIII GGCC 1 cut(s) 51
Hin1II CATG 1 cut(s) 110
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HinfI GANTC 1 cut(s) 176
Hpy166II GTNNAC 3 cut(s) 25, 38, 157
Hpy188I TCNGA 1 cut(s) 72
Hpy188III TCNNGA 1 cut(s) 107
Hpy8I GTNNAC 3 cut(s) 25, 38, 157
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4V TGCA 1 cut(s) 38
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
HpyF3I CTNAG 2 cut(s) 19, 186
Hsp92II CATG 1 cut(s) 110
Kzo9I GATC 2 cut(s) 124, 180
LpnPI CCDG 7 cut(s) 29, 33, 43, 66, 70, 113, 132
MalI GATC 2 cut(s) 126, 182
MboI GATC 2 cut(s) 124, 180
MboII GAAGA 1 cut(s) 158
MhlI GDGCHC 1 cut(s) 40
MlsI TGGCCA 1 cut(s) 51
MluNI TGGCCA 1 cut(s) 51
MlyI GAGTC 1 cut(s) 185
Mox20I TGGCCA 1 cut(s) 51
MscI TGGCCA 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 51
MspA1I CMGCKG 1 cut(s) 122
MspR9I CCNGG 1 cut(s) 58
MvaI CCWGG 1 cut(s) 58
MwoI GCNNNNNNNGC 1 cut(s) 44
NdeII GATC 2 cut(s) 124, 180
NlaIII CATG 1 cut(s) 110
PagI TCATGA 1 cut(s) 106
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PvuII CAGCTG 1 cut(s) 122
Sau3AI GATC 2 cut(s) 124, 180
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 58
SduI GDGCHC 1 cut(s) 40
SetI ASST 2 cut(s) 124, 154
StyD4I CCNGG 1 cut(s) 56
TaaI ACNGT 1 cut(s) 142
TaqI TCGA 2 cut(s) 144, 179
VneI GTGCAC 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.