AT1G12970

Plant Intracellular

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0023638
Physical Location & Seq
Forward (+)
4423540 .. 4425936
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G12970.1

Sequence Viewer

Length: 1395 bp
ATGGATCATGATCTCGAGATCTTCCCTTTACTCTCCTACGTCCTCCACCATTCCGATCCCGCTTCTCACGCGCCACCGTCTCTAGCGATTCAGCAATCTCTTGCGAATCGTTATCCCCTTCTCACCAATCCTTATGTAATCTCCTCGTTGATTGAATCAATTCCCAGTACAATCACGCAGACCCTTTTCGTATTTGGCTCTCTTGGGCCACGACCTGATCCTTTAGCTGTCTCTTCTGCTCGGTCTAAGATCCGAGAGATCAAAGAGAATGATTCTTTATCGCCGGAAGATGCGGCTAAGGAAGAGCAGGTTTACGCTGCGGTTGTGAGTTTAGAGGAAGTTCATGAAGGATATGAGAAGCAGTTAAGAGATTTGGAGGAGGAGATTGGTAGGGTTTATGCTTCGGCGGTTGAATCATTGAGCGGTGGTGATGAAGTGAATGAGGAGGTTTTAGCGGTGATTAAAGACGCTGAAGATGGTGGAGTCGTTGAAAGGATTGATCTTTCTGATCATGAGCTTAAGCTTCTTCCTGATGCTTTAGGGAAAATCGTCGGTTTGGTTTCACTGAATGTCTCTCGTAACAATCTCAGGTTTCTACCTGATACAATCTCAGGACTTGAGAAACTGGAGGAGCTAGATTTATCTTCAAACCGTCTCGTGTTTCTTCCCGACTCTATTGGACTGTTACTTAATTTGAGGATCTTAAATGTGACGGGGAACAAGCTAACTTTACTCCCAGAAAGCATTGCGCAATGCAGGTCACTGGTAGAGTTAGATGCCAGCTTCAACAACTTGACTTCTTTGCCTGCAAACTTCGGATACGGATTACTCAATCTCGAAAGGCTATCGATCCAGCTGAACAAAATCCGTTTCTTCCCCAATTCCATATGTGAGATGCGTTCTCTAAGGTATCTCGACGCACACATGAACGAAATCCACGGCCTTCCAATCGCTATAGGGAGACTCACAAACCTTGAAGTCATGAACTTGAGCAGCAACTTCAGCGACTTGATTGAACTTCCTGACACAATCTCTGATTTAGCTAACTTGAGGGAGCTAGATCTCAGCAACAACCAGATCAGAGTCTTGCCTGACTCGTTTTTCAGGCTGGAGAAGCTGGAGAAACTGAACTTGGACCAGAACCCTTTGGAGTATCCCCCACAAGAGATGGTTAACCAAAGCGCTGAAGCAGTTCGAGAGTTCATGAGGAAGAGGTGGGAAGAGATGGTGGAGGAAGAGCAATTGAGGAGTGTGATTGAAGCTGAGAAGCAACAAGGAGGAGCAACAGGTTGGCTCTCATGGGGGAGCTCCATTGTTACTAGCCTTTTCTCTGGAGGTACTCATGGTGGTGCAGCTAAGAAGCCTAAAAACTCATTTTTGGATGAACAGCTCTGA

Protein Analysis

464

Amino Acids

51.65

Weight (kDa)

4.69

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 165 - 219 2.5e-07 Leucine rich repeat
LRR_8 PF13855 208 - 265 1.5e-08 Leucine rich repeat
LRR_8 PF13855 253 - 312 1e-08 Leucine rich repeat
LRR_14 PF23598 319 - 381 6e-07 Leucine-rich repeat region
LRR_8 PF13855 324 - 383 8e-10 Leucine rich repeat
LRR_4 PF12799 349 - 383 2e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0023638)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12970 AT3G26500
prunus_persica Prupe.1G244900_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 750
Acc36I ACCTGC 2 cut(s) 298, 747
AccBSI CCGCTC 1 cut(s) 423
AccII CGCG 1 cut(s) 71
AciI CCGC 6 cut(s) 60, 293, 320, 407, 423, 455
AclWI GGATC 6 cut(s) 12, 50, 212, 244, 707, 844
AcuI CTGAAG 3 cut(s) 492, 985, 1206
AfaI GTAC 2 cut(s) 169, 1339
AfeI AGCGCT 1 cut(s) 1183
AflII CTTAAG 1 cut(s) 518
AgsI TTSAA 8 cut(s) 155, 413, 491, 648, 787, 977, 1016, 1259
AjuI GAANNNNNNNTTGG 2 cut(s) 1115, 1147
Alw21I GWGCWC 1 cut(s) 1310
Alw26I GTCTC 5 cut(s) 84, 235, 577, 659, 955
AlwI GGATC 6 cut(s) 12, 50, 212, 244, 707, 844
Ama87I CYCGRG 1 cut(s) 14
Aor51HI AGCGCT 1 cut(s) 1183
AoxI GGCC 2 cut(s) 206, 940
ApeKI GCWGC 3 cut(s) 317, 993, 1352
Asp700I GAANNNNTTC 2 cut(s) 159, 1191
AspLEI GCGC 3 cut(s) 73, 751, 1184
AspS9I GGNCC 2 cut(s) 206, 1135
AsuHPI GGTGA 3 cut(s) 115, 440, 469
AvaI CYCGRG 1 cut(s) 14
AvaII GGWCC 1 cut(s) 1135
BanII GRGCYC 1 cut(s) 1310
BauI CACGAG 1 cut(s) 656
Bbv12I GWGCWC 1 cut(s) 1310
BbvI GCAGC 3 cut(s) 304, 1005, 1364
BccI CCATC 3 cut(s) 470, 1162, 1219
BceAI ACGGC 1 cut(s) 955
BciVI GTATCC 2 cut(s) 812, 1164
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 5 cut(s) 84, 235, 577, 659, 955
BfaI CTAG 4 cut(s) 83, 635, 1058, 1320
BfmI CTRYAG 1 cut(s) 954
BfoI RGCGCY 1 cut(s) 1185
BfrI CTTAAG 1 cut(s) 518
BfuAI ACCTGC 2 cut(s) 298, 747
BfuI GTATCC 2 cut(s) 812, 1164
BglII AGATCT 2 cut(s) 18, 1060
BisI GCNGC 4 cut(s) 294, 318, 994, 1353
BlsI GCNGC 4 cut(s) 295, 319, 995, 1354
Bme18I GGWCC 1 cut(s) 1135
BmeT110I CYCGRG 1 cut(s) 14
BmgT120I GGNCC 2 cut(s) 206, 1135
BmrI ACTGGG 1 cut(s) 159
BmsI GCATC 4 cut(s) 280, 523, 766, 885
BmuI ACTGGG 1 cut(s) 159
BpmI CTGGAG 4 cut(s) 647, 1130, 1139, 1353
Bpu10I CCTNAGC 1 cut(s) 297
BpuEI CTTGAG 3 cut(s) 638, 1009, 1069
Bsa29I ATCGAT 1 cut(s) 848
BsaBI GATNNNNATC 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 937
Bse1I ACTGG 3 cut(s) 165, 630, 768
Bse3DI GCAATG 2 cut(s) 744, 758
Bse8I GATNNNNATC 1 cut(s) 9
BseCI ATCGAT 1 cut(s) 848
BseDI CCNNGG 1 cut(s) 937
BseGI GGATG 1 cut(s) 1387
BseJI GATNNNNATC 1 cut(s) 9
BseMI GCAATG 2 cut(s) 744, 758
BseMII CTCAG 4 cut(s) 601, 624, 1078, 1254
BseNI ACTGG 3 cut(s) 165, 630, 768
BseRI GAGGAG 7 cut(s) 133, 392, 395, 458, 644, 1261, 1293
BseXI GCAGC 3 cut(s) 304, 1005, 1364
BsgI GTGCAG 1 cut(s) 1371
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 2 cut(s) 208, 942
BshVI ATCGAT 1 cut(s) 848
BsiHKAI GWGCWC 1 cut(s) 1310
BsiHKCI CYCGRG 1 cut(s) 14
BsiSI CCGG 1 cut(s) 284
BsmAI GTCTC 5 cut(s) 84, 235, 577, 659, 955
BsmBI CGTCTC 2 cut(s) 84, 659
BsnI GGCC 2 cut(s) 208, 942
BsoBI CYCGRG 1 cut(s) 14
Bsp1286I GDGCHC 1 cut(s) 1310
BspACI CCGC 6 cut(s) 60, 293, 320, 407, 423, 455
BspANI GGCC 2 cut(s) 208, 942
BspCNI CTCAG 4 cut(s) 600, 623, 1077, 1255
BspDI ATCGAT 1 cut(s) 848
BspFNI CGCG 1 cut(s) 71
BspHI TCATGA 5 cut(s) 7, 343, 511, 981, 1203
BspMI ACCTGC 2 cut(s) 298, 747
BspPI GGATC 6 cut(s) 12, 50, 212, 244, 707, 844
BspQI GCTCTTC 2 cut(s) 297, 1230
BspTI CTTAAG 1 cut(s) 518
BsrBI CCGCTC 1 cut(s) 423
BsrDI GCAATG 2 cut(s) 744, 758
BsrI ACTGG 3 cut(s) 165, 630, 768
BssECI CCNNGG 1 cut(s) 937
BssSI CACGAG 1 cut(s) 656
Bst2BI CACGAG 1 cut(s) 656
Bst4CI ACNGT 3 cut(s) 78, 653, 684
Bst6I CTCTTC 5 cut(s) 238, 297, 1205, 1215, 1230
BstAFI CTTAAG 1 cut(s) 518
BstC8I GCNNGC 2 cut(s) 781, 807
BstDEI CTNAG 8 cut(s) 246, 297, 587, 610, 905, 1064, 1263, 1356
BstDSI CCRYGG 1 cut(s) 937
BstF5I GGATG 1 cut(s) 1387
BstFNI CGCG 1 cut(s) 71
BstH2I RGCGCY 1 cut(s) 1185
BstHHI GCGC 3 cut(s) 73, 751, 1184
BstMAI GTCTC 5 cut(s) 84, 235, 577, 659, 955
BstMWI GCNNNNNNNGC 3 cut(s) 68, 1002, 1114
BstSFI CTRYAG 1 cut(s) 954
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 3 cut(s) 304, 1005, 1364
BstX2I RGATCY 4 cut(s) 18, 249, 699, 1060
BstYI RGATCY 4 cut(s) 18, 249, 699, 1060
Bsu15I ATCGAT 1 cut(s) 848
BsuI GTATCC 2 cut(s) 812, 1164
BsuRI GGCC 2 cut(s) 208, 942
BsuTUI ATCGAT 1 cut(s) 848
BtgI CCRYGG 1 cut(s) 937
BtsCI GGATG 1 cut(s) 1387
BtsIMutI CAGTG 2 cut(s) 563, 761
BveI ACCTGC 2 cut(s) 298, 747
Cac8I GCNNGC 2 cut(s) 781, 807
CciI TCATGA 5 cut(s) 7, 343, 511, 981, 1203
CfoI GCGC 3 cut(s) 73, 751, 1184
Cfr13I GGNCC 2 cut(s) 206, 1135
ClaI ATCGAT 1 cut(s) 848
CseI GACGC 2 cut(s) 476, 926
Csp6I GTAC 2 cut(s) 168, 1338
CviAII CATG 8 cut(s) 8, 344, 512, 925, 982, 1204, 1299, 1343
CviQI GTAC 2 cut(s) 168, 1338
DdeI CTNAG 8 cut(s) 246, 297, 587, 610, 905, 1064, 1263, 1356
Eam1104I CTCTTC 5 cut(s) 238, 297, 1205, 1215, 1230
EarI CTCTTC 5 cut(s) 238, 297, 1205, 1215, 1230
Ecl136II GAGCTC 1 cut(s) 1308
Eco24I GRGCYC 1 cut(s) 1310
Eco47I GGWCC 1 cut(s) 1135
Eco47III AGCGCT 1 cut(s) 1183
Eco53kI GAGCTC 1 cut(s) 1308
Eco57I CTGAAG 3 cut(s) 492, 985, 1206
Eco88I CYCGRG 1 cut(s) 14
EcoICRI GAGCTC 1 cut(s) 1308
EcoT38I GRGCYC 1 cut(s) 1310
Esp3I CGTCTC 2 cut(s) 84, 659
FaeI CATG 8 cut(s) 11, 347, 515, 928, 985, 1207, 1302, 1346
FatI CATG 8 cut(s) 7, 343, 511, 924, 981, 1203, 1298, 1342
FauI CCCGC 1 cut(s) 67
FauNDI CATATG 1 cut(s) 887
FbaI TGATCA 1 cut(s) 508
Fnu4HI GCNGC 4 cut(s) 294, 318, 994, 1353
FriOI GRGCYC 1 cut(s) 1310
Fsp4HI GCNGC 4 cut(s) 294, 318, 994, 1353
FspBI CTAG 4 cut(s) 83, 635, 1058, 1320
FspI TGCGCA 1 cut(s) 750
GlaI GCGC 3 cut(s) 72, 750, 1183
GluI GCNGC 4 cut(s) 294, 318, 994, 1353
GsuI CTGGAG 4 cut(s) 647, 1130, 1139, 1353
HaeII RGCGCY 1 cut(s) 1185
HaeIII GGCC 2 cut(s) 208, 942
HapII CCGG 1 cut(s) 284
HgaI GACGC 2 cut(s) 476, 926
HhaI GCGC 3 cut(s) 73, 751, 1184
Hin1II CATG 8 cut(s) 11, 347, 515, 928, 985, 1207, 1302, 1346
Hin6I GCGC 3 cut(s) 71, 749, 1182
HinP1I GCGC 3 cut(s) 71, 749, 1182
HincII GTYRAC 1 cut(s) 1174
HindII GTYRAC 1 cut(s) 1174
HindIII AAGCTT 1 cut(s) 521
HpaI GTTAAC 1 cut(s) 1174
HpaII CCGG 1 cut(s) 284
HphI GGTGA 3 cut(s) 115, 440, 469
Hpy166II GTNNAC 2 cut(s) 313, 1174
Hpy188I TCNGA 7 cut(s) 55, 254, 508, 818, 1036, 1082, 1394
Hpy8I GTNNAC 2 cut(s) 313, 1174
Hpy99I CGWCG 2 cut(s) 554, 920
HpyAV CCTTC 3 cut(s) 128, 341, 953
HpyCH4III ACNGT 3 cut(s) 78, 653, 684
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 3 cut(s) 756, 809, 1352
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 1002, 1114
HpyF3I CTNAG 8 cut(s) 246, 297, 587, 610, 905, 1064, 1263, 1356
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 8 cut(s) 11, 347, 515, 928, 985, 1207, 1302, 1346
HspAI GCGC 3 cut(s) 71, 749, 1182
Ksp22I TGATCA 1 cut(s) 508
KspAI GTTAAC 1 cut(s) 1174
LguI GCTCTTC 2 cut(s) 297, 1230
LmnI GCTCC 5 cut(s) 631, 1054, 1280, 1305, 1313
Lsp1109I GCAGC 3 cut(s) 304, 1005, 1364
LweI GCATC 4 cut(s) 280, 523, 766, 885
MaeI CTAG 4 cut(s) 83, 635, 1058, 1320
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 5 cut(s) 578, 684, 709, 759, 1315
MbiI CCGCTC 1 cut(s) 423
MfeI CAATTG 1 cut(s) 1241
MflI RGATCY 4 cut(s) 18, 249, 699, 1060
MhlI GDGCHC 1 cut(s) 1310
MluCI AATT 4 cut(s) 159, 691, 880, 1241
MlyI GAGTC 5 cut(s) 492, 665, 957, 1088, 1092
MroXI GAANNNNTTC 2 cut(s) 159, 1191
MseI TTAA 6 cut(s) 365, 462, 519, 690, 704, 1173
MslI CAYNNNNRTG 1 cut(s) 1347
MspA1I CMGCKG 1 cut(s) 856
MspCI CTTAAG 1 cut(s) 518
MspI CCGG 1 cut(s) 284
MunI CAATTG 1 cut(s) 1241
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 3 cut(s) 68, 1002, 1114
NdeI CATATG 1 cut(s) 887
NlaIII CATG 8 cut(s) 11, 347, 515, 928, 985, 1207, 1302, 1346
NmuCI GTSAC 2 cut(s) 709, 759
NsbI TGCGCA 1 cut(s) 750
PaeR7I CTCGAG 1 cut(s) 14
PagI TCATGA 5 cut(s) 7, 343, 511, 981, 1203
PciSI GCTCTTC 2 cut(s) 297, 1230
PdmI GAANNNNTTC 2 cut(s) 159, 1191
PfeI GAWTC 5 cut(s) 88, 106, 155, 272, 413
PkrI GCNGC 4 cut(s) 295, 319, 995, 1354
PleI GAGTC 5 cut(s) 491, 665, 957, 1088, 1091
PpsI GAGTC 5 cut(s) 491, 665, 957, 1088, 1091
Psp124BI GAGCTC 1 cut(s) 1310
PspPI GGNCC 2 cut(s) 206, 1135
PsuI RGATCY 4 cut(s) 18, 249, 699, 1060
PvuII CAGCTG 1 cut(s) 856
RsaI GTAC 2 cut(s) 169, 1339
RsaNI GTAC 2 cut(s) 168, 1338
RseI CAYNNNNRTG 1 cut(s) 1347
SacI GAGCTC 1 cut(s) 1310
SapI GCTCTTC 2 cut(s) 297, 1230
SaqAI TTAA 6 cut(s) 365, 462, 519, 690, 704, 1173
SatI GCNGC 4 cut(s) 294, 318, 994, 1353
Sau96I GGNCC 2 cut(s) 206, 1135
SchI GAGTC 5 cut(s) 492, 665, 957, 1088, 1092
SduI GDGCHC 1 cut(s) 1310
SfaNI GCATC 4 cut(s) 280, 523, 766, 885
SfcI CTRYAG 1 cut(s) 954
Sfr274I CTCGAG 1 cut(s) 14
SinI GGWCC 1 cut(s) 1135
SlaI CTCGAG 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 1347
SmlI CTYRAG 5 cut(s) 14, 518, 617, 988, 1048
SmoI CTYRAG 5 cut(s) 14, 518, 617, 988, 1048
Sse9I AATT 4 cut(s) 159, 691, 880, 1241
SsiI CCGC 6 cut(s) 60, 293, 320, 407, 423, 455
SspMI CTAG 4 cut(s) 83, 635, 1058, 1320
SstI GAGCTC 1 cut(s) 1310
TaaI ACNGT 3 cut(s) 78, 653, 684
TaiI ACGT 1 cut(s) 42
TaqI TCGA 5 cut(s) 15, 837, 848, 915, 1195
TaqII GACCGA 1 cut(s) 231
TasI AATT 4 cut(s) 159, 691, 880, 1241
TatI WGTACW 1 cut(s) 167
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 5 cut(s) 88, 106, 155, 272, 413
Tru1I TTAA 6 cut(s) 365, 462, 519, 690, 704, 1173
Tru9I TTAA 6 cut(s) 365, 462, 519, 690, 704, 1173
TscAI CASTG 2 cut(s) 570, 768
TseFI GTSAC 2 cut(s) 709, 759
TseI GCWGC 3 cut(s) 317, 993, 1352
Tsp45I GTSAC 2 cut(s) 709, 759
TspDTI ATGAA 6 cut(s) 332, 360, 447, 941, 998, 1192
TspGWI ACGGA 2 cut(s) 837, 857
TspRI CASTG 2 cut(s) 570, 768
Vha464I CTTAAG 1 cut(s) 518
VpaK11BI GGWCC 1 cut(s) 1135
XhoI CTCGAG 1 cut(s) 14
XmnI GAANNNNTTC 2 cut(s) 159, 1191
XspI CTAG 4 cut(s) 83, 635, 1058, 1320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.