AT3G26500

Plant Intracellular

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0023638
Physical Location & Seq
Reverse (-)
9707912 .. 9710100
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G26500.1

Sequence Viewer

Length: 1416 bp
ATGGATCACGATCTCGACAAATTCCCTTTACTCTCTTACGTCCTTCATCAACATGACTCCAATCTCCACGCGCCGCCGTCTATGGCCGCGCAAGAAACTCTCCTTCCTTCTTTTCCTCTTCTCTCCAATCCAGAAATCATGTCTATGTTGACTCAATCCATCCCCACAACGATCACACAGACGCTTTTCGTCTTCAACTCTCTCGGCTCTCGACCTGATCCCTTAGCGGTTTCCTCTGCTCGATTCAAGATCGCTCAAATTATGGATTCGCTCTCGCCGGAGGAAGCAGCGAAGGAGTCGGAGATTTATGCCGGGGTTGTGCGGTTGGATGAAGTGCACGATAGTTACGAGAAGAAGTTGAAGGATACAGAGGAGGAGCTTTCTAGGGTTTATTCTACGGAGGTGGAATCGATGTTGAGAAGCGGTGAAGAAGTGAACGAGAAGGTTCTTGCGGTGCTCAAGGAGGCGGAATCCGGCGGAACCGTTGAGAGGATTGATCTCTCCAGCCAAGAACTTAAGCTTATCCCTGAAGCTTTTTGGAAAGTAGTTGGCTTAGTATACTTGAATCTCTCTGGCAATGACTTGACGTTTATACCGGATGCAATCTCGAAACTCAAGAAGCTAGAAGAGCTCGATGTATCTTCAAATTCTTTGGAATCTCTTCCTGACTCTATAGGGATGTTGCTTAACCTTAGAATCCTTAATGTGAATGCGAACAATCTAACCGCACTCCCTGAGAGCATCGCGCATTGCAGGTCACTAGTTGAGTTAGATGCAAGCTACAACAACTTGACTTCATTACCTACAAACATTGGATACGGATTACAAAACCTCGAGAGATTATCGATCCAGCTGAACAAACTCCGTTACTTTCCAGGTTCGATAAGTGAAATGTATAATCTAAAGTATCTTGACGCACACATGAACGAGATCCACGGGATACCAAACTCGATCGGGAGACTAACGAAACTTGAGGTTCTAAACCTAAGCAGCAATTTCAACAACTTGATGGGTGTCCCTGACACTATCACTGATCTAACTAACCTGAGGGAGCTTGATCTAAGCAACAACCAAATCCAAGCTATACCAGACTCATTTTATAGGCTGAGGAAGCTAGAGAAACTGAACTTGGACCAAAACCCGCTTGAGATCCCATCTCAAGAAGTGGCTACTCAAGGAGCTGAAGTTGTTAGAGAGTTTATGAGGAAGAGATGGGGTGATATCATGGCAGAGCAGCAACAGAGGATTGGCGTTGAGGCTGAGCGACATGGAGATGAAAACGGGTGGGTCTATTGGGGAACCTCCATGGTTACTAATCTTGTTTCTGGAGTGACCCATACCATTGGATTTGGAGGAGCAACTAGCGATGGTGGAGATAAGAAACCGGGAGACTCGTACTTTTATCACCAAATCTGA

Protein Analysis

471

Amino Acids

52.6

Weight (kDa)

4.82

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 178 - 258 5.3e-11 Leucine-rich repeat region
LRR_4 PF12799 185 - 222 1.9e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 207 - 264 4.1e-09 Leucine rich repeat
LRR_8 PF13855 252 - 311 1.1e-07 Leucine rich repeat
LRR_14 PF23598 261 - 331 5.8e-07 Leucine-rich repeat region
LRR_8 PF13855 334 - 382 1.7e-10 Leucine rich repeat
LRR_4 PF12799 347 - 382 1.6e-07 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0023638)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12970 AT3G26500
prunus_persica Prupe.1G244900_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 744
AccI GTMKAC 1 cut(s) 558
AccII CGCG 3 cut(s) 71, 89, 746
AclWI GGATC 5 cut(s) 12, 212, 841, 925, 1144
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 2 cut(s) 20, 646
AcuI CTGAAG 2 cut(s) 549, 1203
AfaI GTAC 1 cut(s) 1397
AfiI CCNNNNNNNGG 1 cut(s) 489
AflII CTTAAG 1 cut(s) 515
AgsI TTSAA 6 cut(s) 196, 247, 361, 565, 645, 1000
AhlI ACTAGT 1 cut(s) 760
AjnI CCWGG 1 cut(s) 874
AjuI GAANNNNNNNTTGG 2 cut(s) 1112, 1144
Alw21I GWGCWC 3 cut(s) 339, 459, 633
Alw26I GTCTC 2 cut(s) 952, 1383
Alw44I GTGCAC 1 cut(s) 335
AlwI GGATC 5 cut(s) 12, 212, 841, 925, 1144
Ama87I CYCGRG 1 cut(s) 833
AoxI GGCC 1 cut(s) 84
ApaLI GTGCAC 1 cut(s) 335
ApeKI GCWGC 3 cut(s) 287, 990, 1234
ApoI RAATTY 2 cut(s) 20, 646
Asp700I GAANNNNTTC 1 cut(s) 660
AspLEI GCGC 3 cut(s) 73, 91, 748
AspS9I GGNCC 1 cut(s) 1132
AsuC2I CCSGG 2 cut(s) 313, 1386
AsuHPI GGTGA 3 cut(s) 437, 1229, 1397
AvaI CYCGRG 1 cut(s) 833
AvaII GGWCC 1 cut(s) 1132
AxyI CCTNAGG 1 cut(s) 1046
BaeGI GKGCMC 1 cut(s) 339
BanII GRGCYC 1 cut(s) 633
BbsI GAAGAC 1 cut(s) 184
Bbv12I GWGCWC 3 cut(s) 339, 459, 633
BbvCI CCTCAGC 1 cut(s) 1106
BbvI GCAGC 3 cut(s) 299, 1002, 1246
BccI CCATC 5 cut(s) 167, 1003, 1162, 1206, 1361
BceAI ACGGC 1 cut(s) 61
BciT130I CCWGG 1 cut(s) 876
BciVI GTATCC 3 cut(s) 358, 809, 933
BcnI CCSGG 2 cut(s) 313, 1386
BcoDI GTCTC 2 cut(s) 952, 1383
BcuI ACTAGT 1 cut(s) 760
BfaI CTAG 5 cut(s) 384, 623, 761, 1115, 1362
BfmI CTRYAG 1 cut(s) 672
BfrI CTTAAG 1 cut(s) 515
BfuAI ACCTGC 1 cut(s) 744
BfuI GTATCC 3 cut(s) 358, 809, 933
BisI GCNGC 5 cut(s) 74, 87, 288, 991, 1235
BlpI GCTNAGC 1 cut(s) 1260
BlsI GCNGC 5 cut(s) 75, 88, 289, 992, 1236
Bme1390I CCNGG 3 cut(s) 313, 876, 1386
Bme18I GGWCC 1 cut(s) 1132
BmeT110I CYCGRG 1 cut(s) 833
BmgT120I GGNCC 1 cut(s) 1132
BmiI GGNNCC 2 cut(s) 481, 1300
BmrFI CCNGG 3 cut(s) 313, 876, 1386
BmsI GCATC 3 cut(s) 589, 750, 763
BpiI GAAGAC 1 cut(s) 184
BpmI CTGGAG 2 cut(s) 487, 1347
Bpu10I CCTNAGC 3 cut(s) 223, 986, 1106
Bpu1102I GCTNAGC 1 cut(s) 1260
BpuEI CTTGAG 6 cut(s) 443, 599, 992, 1143, 1158, 1166
BpuMI CCSGG 2 cut(s) 313, 1386
Bsa29I ATCGAT 2 cut(s) 410, 845
BsaBI GATNNNNATC 1 cut(s) 9
BsaJI CCNNGG 3 cut(s) 312, 934, 1305
BsaWI WCCGGW 1 cut(s) 595
BsaXI ACNNNNNCTCC 2 cut(s) 1170, 1200
Bsc4I CCNNNNNNNGG 1 cut(s) 489
Bse21I CCTNAGG 1 cut(s) 1046
Bse3DI GCAATG 2 cut(s) 583, 748
Bse8I GATNNNNATC 1 cut(s) 9
BseBI CCWGG 1 cut(s) 876
BseCI ATCGAT 2 cut(s) 410, 845
BseDI CCNNGG 3 cut(s) 312, 934, 1305
BseGI GGATG 4 cut(s) 159, 334, 604, 684
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 489
BseMI GCAATG 2 cut(s) 583, 748
BseMII CTCAG 4 cut(s) 726, 1037, 1097, 1251
BseRI GAGGAG 3 cut(s) 386, 389, 1368
BseSI GKGCMC 1 cut(s) 339
BseXI GCAGC 3 cut(s) 299, 1002, 1246
Bsh1236I CGCG 3 cut(s) 71, 89, 746
Bsh1285I CGRYCG 1 cut(s) 954
BshFI GGCC 1 cut(s) 86
BshVI ATCGAT 2 cut(s) 410, 845
BsiEI CGRYCG 1 cut(s) 954
BsiHKAI GWGCWC 3 cut(s) 339, 459, 633
BsiHKCI CYCGRG 1 cut(s) 833
BsiSI CCGG 5 cut(s) 278, 312, 474, 596, 1385
BslFI GGGAC 1 cut(s) 1001
BslI CCNNNNNNNGG 1 cut(s) 489
BsmAI GTCTC 2 cut(s) 952, 1383
BsmFI GGGAC 1 cut(s) 1001
BsmI GAATGC 1 cut(s) 715
BsnI GGCC 1 cut(s) 86
BsoBI CYCGRG 1 cut(s) 833
Bsp1286I GDGCHC 3 cut(s) 339, 459, 633
Bsp1720I GCTNAGC 1 cut(s) 1260
Bsp19I CCATGG 1 cut(s) 1305
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 4 cut(s) 727, 1038, 1098, 1252
BspDI ATCGAT 2 cut(s) 410, 845
BspFNI CGCG 3 cut(s) 71, 89, 746
BspLI GGNNCC 2 cut(s) 481, 1300
BspMI ACCTGC 1 cut(s) 744
BspPI GGATC 5 cut(s) 12, 212, 841, 925, 1144
BspQI GCTCTTC 1 cut(s) 621
BspTI CTTAAG 1 cut(s) 515
BsrDI GCAATG 2 cut(s) 583, 748
BssECI CCNNGG 3 cut(s) 312, 934, 1305
BssNAI GTATAC 1 cut(s) 559
BssT1I CCWWGG 1 cut(s) 1305
Bst1107I GTATAC 1 cut(s) 559
Bst2UI CCWGG 1 cut(s) 876
Bst4CI ACNGT 1 cut(s) 484
Bst6I CTCTTC 4 cut(s) 123, 621, 666, 1202
BstAFI CTTAAG 1 cut(s) 515
BstC8I GCNNGC 1 cut(s) 778
BstDEI CTNAG 9 cut(s) 223, 553, 692, 735, 986, 1046, 1061, 1106, 1260
BstDSI CCRYGG 2 cut(s) 934, 1305
BstF5I GGATG 4 cut(s) 159, 334, 604, 684
BstFNI CGCG 3 cut(s) 71, 89, 746
BstHHI GCGC 3 cut(s) 73, 91, 748
BstMAI GTCTC 2 cut(s) 952, 1383
BstMCI CGRYCG 1 cut(s) 954
BstMWI GCNNNNNNNGC 2 cut(s) 628, 1111
BstNI CCWGG 1 cut(s) 876
BstSCI CCNGG 3 cut(s) 311, 874, 1384
BstSFI CTRYAG 1 cut(s) 672
BstSLI GKGCMC 1 cut(s) 339
BstUI CGCG 3 cut(s) 71, 89, 746
BstV1I GCAGC 3 cut(s) 299, 1002, 1246
BstV2I GAAGAC 1 cut(s) 184
BstX2I RGATCY 2 cut(s) 930, 1149
BstXI CCANNNNNNTGG 1 cut(s) 1343
BstYI RGATCY 2 cut(s) 930, 1149
BstZ17I GTATAC 1 cut(s) 559
Bsu15I ATCGAT 2 cut(s) 410, 845
Bsu36I CCTNAGG 1 cut(s) 1046
BsuI GTATCC 3 cut(s) 358, 809, 933
BsuRI GGCC 1 cut(s) 86
BsuTUI ATCGAT 2 cut(s) 410, 845
BtgI CCRYGG 2 cut(s) 934, 1305
BtgZI GCGATG 2 cut(s) 727, 1380
BtsCI GGATG 4 cut(s) 159, 334, 604, 684
BtsIMutI CAGTG 1 cut(s) 1029
BveI ACCTGC 1 cut(s) 744
Cac8I GCNNGC 1 cut(s) 778
CfoI GCGC 3 cut(s) 73, 91, 748
Cfr13I GGNCC 1 cut(s) 1132
ClaI ATCGAT 2 cut(s) 410, 845
CseI GACGC 2 cut(s) 190, 923
Csp6I GTAC 1 cut(s) 1396
CviAII CATG 6 cut(s) 53, 139, 922, 1225, 1268, 1306
CviQI GTAC 1 cut(s) 1396
DdeI CTNAG 9 cut(s) 223, 553, 692, 735, 986, 1046, 1061, 1106, 1260
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 4 cut(s) 123, 621, 666, 1202
EarI CTCTTC 4 cut(s) 123, 621, 666, 1202
EciI GGCGGA 2 cut(s) 482, 492
Ecl136II GAGCTC 1 cut(s) 631
Eco130I CCWWGG 1 cut(s) 1305
Eco24I GRGCYC 1 cut(s) 633
Eco32I GATATC 1 cut(s) 1222
Eco47I GGWCC 1 cut(s) 1132
Eco53kI GAGCTC 1 cut(s) 631
Eco57I CTGAAG 2 cut(s) 549, 1203
Eco81I CCTNAGG 1 cut(s) 1046
Eco88I CYCGRG 1 cut(s) 833
EcoICRI GAGCTC 1 cut(s) 631
EcoRII CCWGG 1 cut(s) 874
EcoRV GATATC 1 cut(s) 1222
EcoT14I CCWWGG 1 cut(s) 1305
EcoT38I GRGCYC 1 cut(s) 633
ErhI CCWWGG 1 cut(s) 1305
FaeI CATG 6 cut(s) 56, 142, 925, 1228, 1271, 1309
FaqI GGGAC 1 cut(s) 1001
FatI CATG 6 cut(s) 52, 138, 921, 1224, 1267, 1305
FauI CCCGC 1 cut(s) 1149
FblI GTMKAC 1 cut(s) 558
Fnu4HI GCNGC 5 cut(s) 74, 87, 288, 991, 1235
FokI GGATG 4 cut(s) 146, 341, 611, 691
FriOI GRGCYC 1 cut(s) 633
Fsp4HI GCNGC 5 cut(s) 74, 87, 288, 991, 1235
FspBI CTAG 5 cut(s) 384, 623, 761, 1115, 1362
GlaI GCGC 3 cut(s) 72, 90, 747
GluI GCNGC 5 cut(s) 74, 87, 288, 991, 1235
GsuI CTGGAG 2 cut(s) 487, 1347
HaeIII GGCC 1 cut(s) 86
HapII CCGG 5 cut(s) 278, 312, 474, 596, 1385
HgaI GACGC 2 cut(s) 190, 923
HhaI GCGC 3 cut(s) 73, 91, 748
Hin1II CATG 6 cut(s) 56, 142, 925, 1228, 1271, 1309
Hin6I GCGC 3 cut(s) 71, 89, 746
HinP1I GCGC 3 cut(s) 71, 89, 746
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HindIII AAGCTT 2 cut(s) 518, 531
HpaII CCGG 5 cut(s) 278, 312, 474, 596, 1385
HphI GGTGA 3 cut(s) 437, 1229, 1397
Hpy166II GTNNAC 4 cut(s) 150, 337, 436, 559
Hpy188I TCNGA 2 cut(s) 301, 1415
Hpy8I GTNNAC 4 cut(s) 150, 337, 436, 559
HpyAV CCTTC 6 cut(s) 53, 113, 117, 286, 355, 436
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4IV ACGT 2 cut(s) 39, 587
HpyCH4V TGCA 4 cut(s) 337, 602, 753, 776
HpyF10VI GCNNNNNNNGC 2 cut(s) 628, 1111
HpyF3I CTNAG 9 cut(s) 223, 553, 692, 735, 986, 1046, 1061, 1106, 1260
HpySE526I ACGT 2 cut(s) 39, 587
Hsp92II CATG 6 cut(s) 56, 142, 925, 1228, 1271, 1309
HspAI GCGC 3 cut(s) 71, 89, 746
LguI GCTCTTC 1 cut(s) 621
LmnI GCTCC 4 cut(s) 376, 1051, 1178, 1355
Lsp1109I GCAGC 3 cut(s) 299, 1002, 1246
LweI GCATC 3 cut(s) 589, 750, 763
MaeI CTAG 5 cut(s) 384, 623, 761, 1115, 1362
MaeII ACGT 2 cut(s) 39, 587
MaeIII GTNAC 5 cut(s) 344, 756, 866, 1309, 1330
MboII GAAGA 8 cut(s) 110, 184, 364, 440, 633, 638, 653, 1219
MflI RGATCY 2 cut(s) 930, 1149
MhlI GDGCHC 3 cut(s) 339, 459, 633
MluCI AATT 4 cut(s) 20, 258, 646, 994
MlyI GAGTC 6 cut(s) 50, 145, 305, 662, 1085, 1385
MmeI TCCRAC 2 cut(s) 279, 306
MroXI GAANNNNTTC 1 cut(s) 660
MseI TTAA 3 cut(s) 516, 687, 702
MslI CAYNNNNRTG 3 cut(s) 51, 143, 1272
MspA1I CMGCKG 1 cut(s) 853
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 5 cut(s) 278, 312, 474, 596, 1385
MspR9I CCNGG 3 cut(s) 313, 876, 1386
Mva1269I GAATGC 1 cut(s) 715
MvaI CCWGG 1 cut(s) 876
MvnI CGCG 3 cut(s) 71, 89, 746
MwoI GCNNNNNNNGC 2 cut(s) 628, 1111
NciI CCSGG 2 cut(s) 313, 1386
NcoI CCATGG 1 cut(s) 1305
NlaIII CATG 6 cut(s) 56, 142, 925, 1228, 1271, 1309
NlaIV GGNNCC 2 cut(s) 481, 1300
NmeAIII GCCGAG 1 cut(s) 183
NmuCI GTSAC 2 cut(s) 756, 1330
PaeR7I CTCGAG 1 cut(s) 833
PciSI GCTCTTC 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PctI GAATGC 1 cut(s) 715
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 7 cut(s) 243, 266, 407, 470, 565, 656, 696
PkrI GCNGC 5 cut(s) 75, 88, 289, 992, 1236
Ple19I CGATCG 1 cut(s) 954
PleI GAGTC 6 cut(s) 50, 145, 304, 662, 1085, 1385
PpsI GAGTC 6 cut(s) 50, 145, 304, 662, 1085, 1385
Psp124BI GAGCTC 1 cut(s) 633
Psp6I CCWGG 1 cut(s) 874
PspGI CCWGG 1 cut(s) 874
PspN4I GGNNCC 2 cut(s) 481, 1300
PspPI GGNCC 1 cut(s) 1132
PsuI RGATCY 2 cut(s) 930, 1149
PvuI CGATCG 1 cut(s) 954
PvuII CAGCTG 1 cut(s) 853
RsaI GTAC 1 cut(s) 1397
RsaNI GTAC 1 cut(s) 1396
RseI CAYNNNNRTG 3 cut(s) 51, 143, 1272
SacI GAGCTC 1 cut(s) 633
SapI GCTCTTC 1 cut(s) 621
SaqAI TTAA 3 cut(s) 516, 687, 702
SatI GCNGC 5 cut(s) 74, 87, 288, 991, 1235
Sau96I GGNCC 1 cut(s) 1132
SchI GAGTC 6 cut(s) 50, 145, 305, 662, 1085, 1385
ScrFI CCNGG 3 cut(s) 313, 876, 1386
SduI GDGCHC 3 cut(s) 339, 459, 633
SfaNI GCATC 3 cut(s) 589, 750, 763
SfcI CTRYAG 1 cut(s) 672
Sfr274I CTCGAG 1 cut(s) 833
SinI GGWCC 1 cut(s) 1132
SlaI CTCGAG 1 cut(s) 833
SmiMI CAYNNNNRTG 3 cut(s) 51, 143, 1272
SmlI CTYRAG 8 cut(s) 458, 515, 614, 833, 971, 1145, 1158, 1173
SmoI CTYRAG 8 cut(s) 458, 515, 614, 833, 971, 1145, 1158, 1173
SpeI ACTAGT 1 cut(s) 760
Sse9I AATT 4 cut(s) 20, 258, 646, 994
SspMI CTAG 5 cut(s) 384, 623, 761, 1115, 1362
SstI GAGCTC 1 cut(s) 633
StyD4I CCNGG 3 cut(s) 311, 874, 1384
StyI CCWWGG 1 cut(s) 1305
TaaI ACNGT 1 cut(s) 484
TaiI ACGT 2 cut(s) 42, 590
TasI AATT 4 cut(s) 20, 258, 646, 994
TauI GCSGC 2 cut(s) 76, 89
TfiI GAWTC 7 cut(s) 243, 266, 407, 470, 565, 656, 696
Tru1I TTAA 3 cut(s) 516, 687, 702
Tru9I TTAA 3 cut(s) 516, 687, 702
TscAI CASTG 1 cut(s) 1036
TseFI GTSAC 2 cut(s) 756, 1330
TseI GCWGC 3 cut(s) 287, 990, 1234
Tsp45I GTSAC 2 cut(s) 756, 1330
TspDTI ATGAA 5 cut(s) 35, 345, 786, 938, 1290
TspGWI ACGGA 3 cut(s) 413, 834, 854
TspRI CASTG 1 cut(s) 1036
Vha464I CTTAAG 1 cut(s) 515
VneI GTGCAC 1 cut(s) 335
VpaK11BI GGWCC 1 cut(s) 1132
XapI RAATTY 2 cut(s) 20, 646
XhoI CTCGAG 1 cut(s) 833
XmiI GTMKAC 1 cut(s) 558
XmnI GAANNNNTTC 1 cut(s) 660
XspI CTAG 5 cut(s) 384, 623, 761, 1115, 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.