AT1G33350

PPR repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0020959
Physical Location & Seq
Reverse (-)
12089249 .. 12091743
2495 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G33350.2

Sequence Viewer

Length: 1617 bp
ATGGGGACACGTGTAACACAATTCTCTTACCTCCACGCGCCGTCGTCACACATGGCGGAGCAGTTACTGAATCAATTCATCTCCGCAGTGATATCAAAGTCGAGACATTTGAATCATTTGAAGCAAGTTCAGTCCTTTATGATAGTTTCTGGACTAAGTCATTCACATTTCCTCTGTTTTAAGCTTCTTCGTTTTTGTACGCTTCGTCTCTGTAATCTCTCTTACGCTCGCTTTATCTTTGATCGTTTCTCTTTCCCCAATACGCATCTTTACGCTGCCGTTTTAACTGCTTACTCTTCTTCTCTTCCTCTTCATGCTTCTTCAGCTTTCTCCTTCTTTCGTCTCATGGTTAATCGTTCTGTTCCTCGGCCTAATCACTTTATCTACCCACTTGTTCTTAAGTCTACACCATATCTCTCCTCTGCTTTTTCAACTCCTTTGGTGCATACCCATTTGTTTAAATCTGGGTTTCATCTCTATGTCGTTGTTCAAACTGCTCTTCTTCACTCCTACGCTTCCTCTGTTTCTCATATAACTCTTGCAAGACAACTGTTCGACGAAATGTCTGAGAGAAATGTTGTTTCTTGGACTGCTATGCTTTCTGGGTATGCTAGGTCTGGTGATATCTCTAATGCTGTTGCTCTTTTTGAAGATATGCCTGAGCGTGATGTTCCTTCCTGGAATGCGATTCTTGCTGCTTGTACTCAGAATGGTCTTTTTCTTGAAGCGGTTTCTCTGTTTAGAAGGATGATTAATGAGCCTAGCATTCGTCCTAATGAGGTAACTGTAGTTTGTGTCCTCTCTGCGTGTGCTCAAACTGGTACGCTTCAGCTGGCTAAAGGGATTCATGCCTTTGCTTACCGTCGTGATCTTTCTTCTGATGTGTTTGTTTCGAATTCTTTAGTTGACTTGTATGGAAAATGTGGTAACTTGGAGGAAGCAAGCTCTGTTTTTAAGATGGCATCCAAGAAAAGTTTAACAGCTTGGAATTCTATGATCAATTGCTTTGCACTCCATGGTAGAAGTGAAGAAGCCATAGCTGTGTTTGAAGAAATGATGAAGTTGAACATTAACGATATCAAACCAGATCACATTACTTTCATCGGTCTTCTCAATGCGTGTACTCATGGAGGTTTGGTATCAAAAGGCCGAGGTTATTTCGATCTGATGACAAACAGATTCGGTATTGAGCCAAGGATTGAGCATTATGGCTGTCTAATAGATCTTTTAGGCAGGGCAGGTCGGTTTGATGAAGCTTTAGAAGTTATGTCCACGATGAAGATGAAAGCGGATGAGGCTATTTGGGGATCACTGCTTAATGCTTGTAAGATACATGGCCATCTGGATTTGGCTGAAGTTGCTGTAAAAAACCTGGTTGCTTTAAATCCTAATAATGGAGGTTATGTCGCTATGATGGCAAATTTATATGGAGAGATGGGTAATTGGGAAGAGGCACGTAGAGCTAGAAAGATGATCAAGCACCAAAATGCTTATAAACCACCAGGCTGGAGTCGGATTGAGATCGATAACGAGGTCCATCAGTTCTACTCTCTTGACAAATCACATCCGGAGACTGAGGAGATATACATGATACTTGACAGTCTGATCTCTTTCTGA

Protein Analysis

538

Amino Acids

60.47

Weight (kDa)

8.22

Isoelectric Point (pI)

45.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 191 - 224 3.3e-07 PPR repeat family
PPR PF01535 194 - 223 2.7e-07 PPR repeat
PPR_2 PF13041 223 - 271 9.9e-07 PPR repeat family
PPR_2 PF13041 327 - 375 8.5e-09 PPR repeat family
PPR PF01535 328 - 357 5.6e-08 PPR repeat
E_motif PF20431 445 - 507 1e-10 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020959)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33350 AT1G33350
malus_domestica MD11G1207700.v1.1
prunus_persica Prupe.8G000500_v2.0.a1
pyrus_communis pycom11g18090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1494
Acc36I ACCTGC 1 cut(s) 1229
AccI GTMKAC 1 cut(s) 404
AccII CGCG 1 cut(s) 38
AccIII TCCGGA 1 cut(s) 1567
AciI CCGC 4 cut(s) 56, 84, 728, 1289
AclWI GGATC 1 cut(s) 1315
AcoI YGGCCR 1 cut(s) 1336
AcsI RAATTY 3 cut(s) 895, 988, 1420
AcuI CTGAAG 3 cut(s) 306, 812, 1374
AcvI CACGTG 1 cut(s) 11
AfaI GTAC 4 cut(s) 199, 703, 823, 1123
AfiI CCNNNNNNNGG 1 cut(s) 1394
AflII CTTAAG 1 cut(s) 398
AflIII ACRYGT 2 cut(s) 8, 10
AgsI TTSAA 8 cut(s) 112, 121, 432, 491, 650, 725, 1049, 1066
AhdI GACNNNNNGTC 1 cut(s) 562
AjnI CCWGG 3 cut(s) 677, 1371, 1501
AluBI AGCT 8 cut(s) 184, 326, 832, 945, 983, 1040, 1256, 1463
AluI AGCT 8 cut(s) 184, 326, 832, 945, 983, 1040, 1256, 1463
Alw21I GWGCWC 1 cut(s) 814
Alw26I GTCTC 4 cut(s) 97, 212, 347, 1565
AlwI GGATC 1 cut(s) 1315
AlwNI CAGNNNCTG 1 cut(s) 67
Aor13HI TCCGGA 1 cut(s) 1567
AoxI GGCC 3 cut(s) 368, 1147, 1336
ApeKI GCWGC 2 cut(s) 275, 695
ApoI RAATTY 3 cut(s) 895, 988, 1420
AseI ATTAAT 1 cut(s) 753
Asp700I GAANNNNTTC 1 cut(s) 74
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 1534
AsuHPI GGTGA 1 cut(s) 632
AsuII TTCGAA 1 cut(s) 893
AvaII GGWCC 1 cut(s) 1534
BalI TGGCCA 1 cut(s) 1338
BbrPI CACGTG 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 1100
Bbv12I GWGCWC 1 cut(s) 814
BbvI GCAGC 2 cut(s) 262, 682
BccI CCATC 5 cut(s) 952, 1347, 1408, 1429, 1545
BceAI ACGGC 2 cut(s) 25, 263
BciT130I CCWGG 3 cut(s) 679, 1373, 1503
BclI TGATCA 2 cut(s) 996, 1473
BcoDI GTCTC 4 cut(s) 97, 212, 347, 1565
BfaI CTAG 3 cut(s) 612, 762, 1464
BfmI CTRYAG 1 cut(s) 786
BfrI CTTAAG 1 cut(s) 398
BfuAI ACCTGC 1 cut(s) 1229
BglII AGATCT 1 cut(s) 1222
BisI GCNGC 2 cut(s) 276, 696
BlsI GCNGC 2 cut(s) 277, 697
Bme1390I CCNGG 3 cut(s) 679, 1373, 1503
Bme18I GGWCC 1 cut(s) 1534
BmeRI GACNNNNNGTC 1 cut(s) 562
BmgT120I GGNCC 1 cut(s) 1534
BmrFI CCNGG 3 cut(s) 679, 1373, 1503
BmsI GCATC 2 cut(s) 274, 971
BpiI GAAGAC 1 cut(s) 1100
BpmI CTGGAG 1 cut(s) 1528
Bpu10I CCTNAGC 1 cut(s) 660
Bpu14I TTCGAA 1 cut(s) 893
Bsa29I ATCGAT 1 cut(s) 1524
BsaAI YACGTR 2 cut(s) 11, 1457
BsaBI GATNNNNATC 1 cut(s) 1520
BsaJI CCNNGG 4 cut(s) 365, 1015, 1150, 1193
BsaWI WCCGGW 1 cut(s) 1567
BsaXI ACNNNNNCTCC 4 cut(s) 1122, 1152, 1389, 1419
Bsc4I CCNNNNNNNGG 1 cut(s) 1394
Bse1I ACTGG 1 cut(s) 823
Bse8I GATNNNNATC 1 cut(s) 1520
BseAI TCCGGA 1 cut(s) 1567
BseBI CCWGG 3 cut(s) 679, 1373, 1503
BseCI ATCGAT 1 cut(s) 1524
BseDI CCNNGG 4 cut(s) 365, 1015, 1150, 1193
BseGI GGATG 4 cut(s) 753, 962, 1297, 1564
BseJI GATNNNNATC 1 cut(s) 1520
BseLI CCNNNNNNNGG 1 cut(s) 1394
BseMII CTCAG 4 cut(s) 558, 651, 719, 1566
BseNI ACTGG 1 cut(s) 823
BseRI GAGGAG 2 cut(s) 409, 1592
BseXI GCAGC 2 cut(s) 262, 682
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 3 cut(s) 370, 1149, 1338
BshVI ATCGAT 1 cut(s) 1524
BsiHKAI GWGCWC 1 cut(s) 814
BsiSI CCGG 1 cut(s) 1568
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 1394
BsmAI GTCTC 4 cut(s) 97, 212, 347, 1565
BsmBI CGTCTC 2 cut(s) 212, 347
BsmFI GGGAC 1 cut(s) 19
BsmI GAATGC 2 cut(s) 688, 765
BsnI GGCC 3 cut(s) 370, 1149, 1338
Bsp119I TTCGAA 1 cut(s) 893
Bsp1286I GDGCHC 1 cut(s) 814
Bsp13I TCCGGA 1 cut(s) 1567
Bsp19I CCATGG 1 cut(s) 1015
BspACI CCGC 4 cut(s) 56, 84, 728, 1289
BspANI GGCC 3 cut(s) 370, 1149, 1338
BspCNI CTCAG 4 cut(s) 559, 652, 718, 1567
BspDI ATCGAT 1 cut(s) 1524
BspEI TCCGGA 1 cut(s) 1567
BspFNI CGCG 1 cut(s) 38
BspMI ACCTGC 1 cut(s) 1229
BspPI GGATC 1 cut(s) 1315
BspQI GCTCTTC 1 cut(s) 504
BspT104I TTCGAA 1 cut(s) 893
BspTI CTTAAG 1 cut(s) 398
BsrI ACTGG 1 cut(s) 823
BssECI CCNNGG 4 cut(s) 365, 1015, 1150, 1193
BssT1I CCWWGG 2 cut(s) 1015, 1193
Bst2UI CCWGG 3 cut(s) 679, 1373, 1503
Bst4CI ACNGT 4 cut(s) 552, 787, 863, 1601
Bst6I CTCTTC 5 cut(s) 301, 309, 315, 504, 1443
BstAFI CTTAAG 1 cut(s) 398
BstBAI YACGTR 2 cut(s) 11, 1457
BstBI TTCGAA 1 cut(s) 893
BstC8I GCNNGC 3 cut(s) 229, 834, 943
BstDEI CTNAG 5 cut(s) 155, 567, 660, 705, 1575
BstDSI CCRYGG 1 cut(s) 1015
BstF5I GGATG 4 cut(s) 753, 962, 1297, 1564
BstFNI CGCG 1 cut(s) 38
BstHHI GCGC 1 cut(s) 40
BstMAI GTCTC 4 cut(s) 97, 212, 347, 1565
BstMWI GCNNNNNNNGC 6 cut(s) 323, 692, 1295, 1358, 1415, 1460
BstNI CCWGG 3 cut(s) 679, 1373, 1503
BstSCI CCNGG 3 cut(s) 677, 1371, 1501
BstSFI CTRYAG 1 cut(s) 786
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 2 cut(s) 262, 682
BstV2I GAAGAC 1 cut(s) 1100
BstX2I RGATCY 1 cut(s) 1222
BstXI CCANNNNNNTGG 1 cut(s) 1506
BstYI RGATCY 1 cut(s) 1222
Bsu15I ATCGAT 1 cut(s) 1524
BsuRI GGCC 3 cut(s) 370, 1149, 1338
BsuTUI ATCGAT 1 cut(s) 1524
BtgI CCRYGG 1 cut(s) 1015
BtsCI GGATG 4 cut(s) 753, 962, 1297, 1564
BtsI GCAGTG 2 cut(s) 93, 1310
BtsIMutI CAGTG 2 cut(s) 93, 1310
BveI ACCTGC 1 cut(s) 1229
Cac8I GCNNGC 3 cut(s) 229, 834, 943
CaiI CAGNNNCTG 1 cut(s) 67
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 1534
ClaI ATCGAT 1 cut(s) 1524
CsiI ACCWGGT 1 cut(s) 1371
Csp6I GTAC 4 cut(s) 198, 702, 822, 1122
CviAII CATG 8 cut(s) 52, 314, 346, 848, 1016, 1127, 1334, 1588
CviQI GTAC 4 cut(s) 198, 702, 822, 1122
DdeI CTNAG 5 cut(s) 155, 567, 660, 705, 1575
DraI TTTAAA 2 cut(s) 460, 1383
DriI GACNNNNNGTC 1 cut(s) 562
EaeI YGGCCR 1 cut(s) 1336
Eam1104I CTCTTC 5 cut(s) 301, 309, 315, 504, 1443
Eam1105I GACNNNNNGTC 1 cut(s) 562
EarI CTCTTC 5 cut(s) 301, 309, 315, 504, 1443
EciI GGCGGA 1 cut(s) 71
Eco130I CCWWGG 2 cut(s) 1015, 1193
Eco32I GATATC 3 cut(s) 93, 625, 1078
Eco47I GGWCC 1 cut(s) 1534
Eco57I CTGAAG 3 cut(s) 306, 812, 1374
Eco72I CACGTG 1 cut(s) 11
EcoRI GAATTC 2 cut(s) 895, 988
EcoRII CCWGG 3 cut(s) 677, 1371, 1501
EcoRV GATATC 3 cut(s) 93, 625, 1078
EcoT14I CCWWGG 2 cut(s) 1015, 1193
ErhI CCWWGG 2 cut(s) 1015, 1193
Esp3I CGTCTC 2 cut(s) 212, 347
FaeI CATG 8 cut(s) 55, 317, 349, 851, 1019, 1130, 1337, 1591
FaqI GGGAC 1 cut(s) 19
FatI CATG 8 cut(s) 51, 313, 345, 847, 1015, 1126, 1333, 1587
FbaI TGATCA 2 cut(s) 996, 1473
FblI GTMKAC 1 cut(s) 404
Fnu4HI GCNGC 2 cut(s) 276, 696
FokI GGATG 4 cut(s) 760, 949, 1304, 1551
Fsp4HI GCNGC 2 cut(s) 276, 696
FspBI CTAG 3 cut(s) 612, 762, 1464
GlaI GCGC 1 cut(s) 39
GluI GCNGC 2 cut(s) 276, 696
GsuI CTGGAG 1 cut(s) 1528
HaeIII GGCC 3 cut(s) 370, 1149, 1338
HapII CCGG 1 cut(s) 1568
HhaI GCGC 1 cut(s) 40
Hin1II CATG 8 cut(s) 55, 317, 349, 851, 1019, 1130, 1337, 1591
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 1 cut(s) 907
HindII GTYRAC 1 cut(s) 907
HindIII AAGCTT 2 cut(s) 182, 1254
HinfI GANTC 6 cut(s) 70, 112, 688, 844, 1179, 1510
HpaII CCGG 1 cut(s) 1568
HphI GGTGA 1 cut(s) 632
Hpy166II GTNNAC 4 cut(s) 405, 907, 1122, 1272
Hpy188I TCNGA 7 cut(s) 568, 708, 880, 1167, 1515, 1605, 1616
Hpy188III TCNNGA 7 cut(s) 102, 150, 722, 866, 1343, 1553, 1568
Hpy8I GTNNAC 4 cut(s) 405, 907, 1122, 1272
Hpy99I CGWCG 3 cut(s) 46, 560, 867
HpyAV CCTTC 3 cut(s) 343, 684, 738
HpyCH4III ACNGT 4 cut(s) 552, 787, 863, 1601
HpyCH4IV ACGT 2 cut(s) 10, 1456
HpyCH4V TGCA 3 cut(s) 445, 542, 1010
HpyF10VI GCNNNNNNNGC 6 cut(s) 323, 692, 1295, 1358, 1415, 1460
HpyF3I CTNAG 5 cut(s) 155, 567, 660, 705, 1575
HpySE526I ACGT 2 cut(s) 10, 1456
Hsp92II CATG 8 cut(s) 55, 317, 349, 851, 1019, 1130, 1337, 1591
HspAI GCGC 1 cut(s) 38
Kpn2I TCCGGA 1 cut(s) 1567
Ksp22I TGATCA 2 cut(s) 996, 1473
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 1 cut(s) 58
Lsp1109I GCAGC 2 cut(s) 262, 682
LweI GCATC 2 cut(s) 274, 971
MabI ACCWGGT 1 cut(s) 1371
MaeI CTAG 3 cut(s) 612, 762, 1464
MaeII ACGT 2 cut(s) 10, 1456
MaeIII GTNAC 5 cut(s) 13, 45, 63, 781, 926
MfeI CAATTG 1 cut(s) 1000
MflI RGATCY 1 cut(s) 1222
MhlI GDGCHC 1 cut(s) 814
MlsI TGGCCA 1 cut(s) 1338
MluCI AATT 7 cut(s) 20, 74, 895, 988, 1000, 1420, 1441
MluNI TGGCCA 1 cut(s) 1338
MlyI GAGTC 1 cut(s) 1519
MmeI TCCRAC 1 cut(s) 1493
Mox20I TGGCCA 1 cut(s) 1338
MroI TCCGGA 1 cut(s) 1567
MroXI GAANNNNTTC 1 cut(s) 74
MscI TGGCCA 1 cut(s) 1338
MslI CAYNNNNRTG 3 cut(s) 477, 1040, 1485
Msp20I TGGCCA 1 cut(s) 1338
MspA1I CMGCKG 1 cut(s) 832
MspCI CTTAAG 1 cut(s) 398
MspI CCGG 1 cut(s) 1568
MspR9I CCNGG 3 cut(s) 679, 1373, 1503
MunI CAATTG 1 cut(s) 1000
Mva1269I GAATGC 2 cut(s) 688, 765
MvaI CCWGG 3 cut(s) 679, 1373, 1503
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 6 cut(s) 323, 692, 1295, 1358, 1415, 1460
NcoI CCATGG 1 cut(s) 1015
NlaIII CATG 8 cut(s) 55, 317, 349, 851, 1019, 1130, 1337, 1591
NmeAIII GCCGAG 2 cut(s) 346, 1175
NmuCI GTSAC 1 cut(s) 45
NspV TTCGAA 1 cut(s) 893
PciSI GCTCTTC 1 cut(s) 504
PctI GAATGC 2 cut(s) 688, 765
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 5 cut(s) 70, 112, 688, 844, 1179
PflFI GACNNNGTC 1 cut(s) 156
PfoI TCCNGGA 1 cut(s) 677
PkrI GCNGC 2 cut(s) 277, 697
PleI GAGTC 1 cut(s) 1518
PmaCI CACGTG 1 cut(s) 11
PmlI CACGTG 1 cut(s) 11
PpsI GAGTC 1 cut(s) 1518
Ppu21I YACGTR 2 cut(s) 11, 1457
PshBI ATTAAT 1 cut(s) 753
PsiI TTATAA 1 cut(s) 1494
Psp6I CCWGG 3 cut(s) 677, 1371, 1501
PspCI CACGTG 1 cut(s) 11
PspGI CCWGG 3 cut(s) 677, 1371, 1501
PspPI GGNCC 1 cut(s) 1534
PstNI CAGNNNCTG 1 cut(s) 67
PsuI RGATCY 1 cut(s) 1222
PsyI GACNNNGTC 1 cut(s) 156
PvuII CAGCTG 1 cut(s) 832
RsaI GTAC 4 cut(s) 199, 703, 823, 1123
RsaNI GTAC 4 cut(s) 198, 702, 822, 1122
RseI CAYNNNNRTG 3 cut(s) 477, 1040, 1485
SapI GCTCTTC 1 cut(s) 504
SatI GCNGC 2 cut(s) 276, 696
Sau96I GGNCC 1 cut(s) 1534
SchI GAGTC 1 cut(s) 1519
ScrFI CCNGG 3 cut(s) 679, 1373, 1503
SduI GDGCHC 1 cut(s) 814
SexAI ACCWGGT 1 cut(s) 1371
SfaNI GCATC 2 cut(s) 274, 971
SfcI CTRYAG 1 cut(s) 786
SfuI TTCGAA 1 cut(s) 893
SinI GGWCC 1 cut(s) 1534
SmiMI CAYNNNNRTG 3 cut(s) 477, 1040, 1485
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 7 cut(s) 20, 74, 895, 988, 1000, 1420, 1441
SsiI CCGC 4 cut(s) 56, 84, 728, 1289
SspMI CTAG 3 cut(s) 612, 762, 1464
StyD4I CCNGG 3 cut(s) 677, 1371, 1501
StyI CCWWGG 2 cut(s) 1015, 1193
TaaI ACNGT 4 cut(s) 552, 787, 863, 1601
TaiI ACGT 2 cut(s) 13, 1459
TaqI TCGA 5 cut(s) 101, 555, 893, 1161, 1524
TaqII GACCGA 1 cut(s) 1094
TasI AATT 7 cut(s) 20, 74, 895, 988, 1000, 1420, 1441
TatI WGTACW 2 cut(s) 701, 1121
TfiI GAWTC 5 cut(s) 70, 112, 688, 844, 1179
TscAI CASTG 2 cut(s) 93, 1317
TseFI GTSAC 1 cut(s) 45
TseI GCWGC 2 cut(s) 275, 695
Tsp45I GTSAC 1 cut(s) 45
TspDTI ATGAA 9 cut(s) 67, 302, 461, 836, 1073, 1090, 1266, 1292, 1298
TspRI CASTG 2 cut(s) 93, 1317
Tth111I GACNNNGTC 1 cut(s) 156
Vha464I CTTAAG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 1534
VspI ATTAAT 1 cut(s) 753
XapI RAATTY 3 cut(s) 895, 988, 1420
XmiI GTMKAC 1 cut(s) 404
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 3 cut(s) 612, 762, 1464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.