AT1G49100

leucine-rich repeat protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0027262
Physical Location & Seq
Reverse (-)
18166128 .. 18170105
3978 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G49100.1

Sequence Viewer

Length: 2667 bp
ATGGAGAAGTATTTTCATGGAGTTTTATGTGTGTTCATCATCACAGTTGCTTTTATACATGTTGTTCAGGCTCAAGATCCAAACGGATTCATCACTTTGGATTGTGGTCTGTTACCTGATGGATCTCCATATACCAATCCATCTACTGGATTAACATTCACTTCGGATTCTAGTTTCATCGAGAGTGGAAAGAATGGCCGAGTCAGTAAGGACTCTGAGCGAAACTTCGAAAAAGCTTTTGTAACTCTAAGATACTTTCCAGATGGAGAGCGGAACTGTTATAACCTGAATGTCACACAAGGAACAAATTACTTGATTAGAGCAGCTTTCTTATATGGAAATTACGATGGTCTTAATACTGTCCCAAACTTTGATCTATTTATTGGCCCTAATAAGGTGACAACAGTGAATTTTAATGCAACCGGAGGTGGTGTGTTCGTGGAGATAATTCACATGTCAAGGTCAACCCCTTTGGATATTTGTCTTGTTAAGACAGGAACAACTACACCGATGATATCAACCTTGGAGCTACGACCTTTGAGAAGTGATACTTACATTAGTGCCATTGGGAGCTCCTTGCTCCTCTATTTTAGAGGTTATCTTAATGATTCAGGTGTCGTTTTACGGTACCCCGATGATGTCAACGACCGTAGATGGTTCCCATTCTCATATAAGGAGTGGAAAATTGTAACCACAACTCTCAATGTAAACACTTCAAATGGTTTTGATCTACCACAAGGTGCAATGGCATCGGCTGCAACCCGTGTTAATGATAATGGGACATGGGAATTTCCATGGAGCTTAGAGGATTCTACCACACGGTTTCACATTTACCTTCACTTCGCAGAGCTTCAAACTTTGTTAGCCAACGAGACTAGAGAATTCAATGTTTTGCTGAATGGAAAAGTTTATTATGGACCTTATAGTCCTAAAATGTTAAGTATAGATACTATGAGCCCCCAACCCGATTCGACATTGACATGTAAAGGAGGAAGTTGCCTCTTGCAGCTAGTGAAGACAACAAAGTCAACTCTTCCTCCTCTCATCAATGCTATTGAACTTTTTACTGTTGTTGAGTTTCCTCAATCAGAAACAAACCAAGATGAAGTGATTGCTATCAAGAAGATCCAACTTACTTATGGATTGAGTAGAATTAACTGGCAAGGAGATCCATGTGTCCCCGAGCAGTTTTTGTGGGCTGGTTTGAAGTGCAGCAATATTAATAGTTCCACTCCACCAACAATCACTTTCTTAAACTTGTCTTCAAGTGGACTAACCGGGATCATTTCACCTTCCATCCAGAATTTGACCCATTTACAAGAGTTGGATTTGTCAAATAACGACTTGACCGGGGATGTGCCTGAGTTTCTAGCTGACATAAAATCGCTCTTGATCATAAACTTAAGTGGAAACAATTTTAGCGGTCAACTTCCTCAAAAGCTTATAGATAAGAAAAGACTGAAGCTGAATGTTGAAGGAAACCCTAAGCTTCTTTGCACAAAAGGACCATGTGGAAATAAACCTGGAGAAGGTGGACATCCCAAAAAGAGTATAATTGTACCGGTTGTCTCATCAGTTGCTTTAATAGCTATTCTTATAGCTGCATTGGTTTTGTTTTTGGTTCTTAGAAAGAAAAATCCATCAAGGAGTAAAGAAAATGGTAGAACTTCAAGATCATCCGAGCCACCAAGAATAACAAAAAAGAAAAAGTTTACTTACGTGGAAGTTACTGAAATGACAAATAACTTTAGAAGTGTTCTTGGGAAAGGAGGGTTCGGTATGGTTTATCATGGATATGTAAATGGTAGAGAGCAAGTTGCTGTTAAAGTACTCTCACACGCTTCAAAACATGGCCATAAACAATTCAAAGCAGAGGTTGAACTTCTTTTGAGAGTTCATCACAAGAATTTGGTAAGCCTAGTTGGATACTGCGAAAAAGGGAAGGAATTGGCTCTTGTCTACGAATACATGGCTAATGGAGACTTAAAAGAGTTTTTCTCAGGGAAGCGTGGTGATGATGTTTTAAGGTGGGAAACTAGATTACAAATAGCAGTGGAGGCCGCACAAGGTTTGGAGTACTTGCATAAAGGATGTAGACCACCAATTGTTCATAGAGATGTCAAAACCGCAAACATATTATTGGATGAACACTTCCAAGCCAAACTTGCTGACTTTGGGCTTTCGAGATCATTTCTAAACGAAGGAGAAAGTCATGTCTCGACAGTTGTTGCAGGAACTATTGGTTACCTTGATCCAGAATATTACAGAACAAATTGGTTGACAGAGAAGAGTGATGTGTATAGTTTTGGGGTCGTTTTATTGGAGATCATAACAAATCAGCGCGTGATTGAGCGGACTCGAGAAAAGCCACACATAGCAGAATGGGTGAATTTAATGATTACCAAAGGAGATATTAGAAAAATTGTAGATCCAAATCTCAAGGGAGATTACCATTCTGATTCTGTTTGGAAGTTTGTGGAGCTAGCAATGACTTGTGTAAATGATTCTTCAGCGACAAGACCGACCATGACTCAAGTTGTTACCGAACTAACCGAATGTGTAACTTTAGAAAACTCAAGGGGAGGGAAAAGTCAGAACATGGGTTCAACGAGTTCAAGCGAAGTGACCATGACCTTTGATACCGAAGTGAACCCTGTGGCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001653 GO:0002229 GO:0002237 GO:0002238 GO:0002239 GO:0002376 GO:0002831 GO:0002832 GO:0002833 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009787 GO:0009788 GO:0009790 GO:0009791 GO:0009793 GO:0009845 GO:0009888 GO:0009960 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010154 GO:0010200 GO:0010243 GO:0010646 GO:0010648 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023051 GO:0023052 GO:0023057 GO:0031347 GO:0031348 GO:0031349 GO:0032101 GO:0032102 GO:0032103 GO:0032501 GO:0032502 GO:0032879 GO:0036211 GO:0038023 GO:0042221 GO:0042493 GO:0042802 GO:0042803 GO:0042995 GO:0043170 GO:0043207 GO:0043412 GO:0043900 GO:0043901 GO:0043902 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0046777 GO:0046983 GO:0048316 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0090351 GO:0090406 GO:0098542 GO:0120025 GO:0140096 GO:1900150 GO:1900424 GO:1900426 GO:1901419 GO:1901420 GO:1901564 GO:1901698 GO:1901700 GO:1902288 GO:1902289 GO:1905957 GO:1905958 GO:2000071
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

888

Amino Acids

98.87

Weight (kDa)

7.55

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 33 - 357 2e-122 Malectin-like domain
LRR_8 PF13855 415 - 473 2.6e-07 Leucine rich repeat
LRR_4 PF12799 437 - 477 9.3e-06 Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr PF07714 583 - 850 3.2e-50 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 585 - 848 1.7e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0027262)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49100 AT3G21340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
AasI GACNNNNNNGTC 2 cut(s) 924, 1024
Acc65I GGTACC 1 cut(s) 627
AccB1I GGYRCC 1 cut(s) 627
AccB7I CCANNNNNTGG 1 cut(s) 146
AccBSI CCGCTC 2 cut(s) 271, 2353
AccI GTMKAC 2 cut(s) 1959, 2095
AccII CGCG 1 cut(s) 2343
AciI CCGC 5 cut(s) 271, 1422, 2061, 2127, 2353
AclWI GGATC 7 cut(s) 71, 130, 1120, 1163, 1289, 2246, 2423
AcoI YGGCCR 2 cut(s) 196, 1852
AcsI RAATTY 6 cut(s) 409, 788, 881, 1303, 1906, 2389
AcuI CTGAAG 2 cut(s) 1481, 2493
AdeI CACNNNGTG 1 cut(s) 740
AfaI GTAC 4 cut(s) 629, 1560, 1830, 2078
AfiI CCNNNNNNNGG 3 cut(s) 146, 394, 1529
AflII CTTAAG 1 cut(s) 1402
AflIII ACRYGT 3 cut(s) 58, 453, 980
AgeI ACCGGT 1 cut(s) 1561
AjnI CCWGG 1 cut(s) 1522
Alw21I GWGCWC 1 cut(s) 575
Alw26I GTCTC 4 cut(s) 866, 1573, 1974, 2221
AlwI GGATC 7 cut(s) 71, 130, 1120, 1163, 1289, 2246, 2423
Ama87I CYCGRG 2 cut(s) 1181, 2358
AoxI GGCC 4 cut(s) 196, 385, 1852, 2058
ApeKI GCWGC 5 cut(s) 323, 755, 1006, 1212, 1601
ApoI RAATTY 6 cut(s) 409, 788, 881, 1303, 1906, 2389
AseI ATTAAT 1 cut(s) 1221
AsiGI ACCGGT 1 cut(s) 1561
Asp718I GGTACC 1 cut(s) 627
AspLEI GCGC 1 cut(s) 2343
AspS9I GGNCC 3 cut(s) 386, 917, 1505
AsuC2I CCSGG 2 cut(s) 1279, 1351
AsuHPI GGTGA 4 cut(s) 409, 1281, 2024, 2398
AsuII TTCGAA 1 cut(s) 228
AsuNHI GCTAGC 1 cut(s) 2482
AvaI CYCGRG 2 cut(s) 1181, 2358
AvaII GGWCC 2 cut(s) 917, 1505
BaeI ACNNNNGTAYC 2 cut(s) 2631, 2664
BalI TGGCCA 1 cut(s) 1854
BanI GGYRCC 1 cut(s) 627
BanII GRGCYC 2 cut(s) 575, 959
BbsI GAAGAC 2 cut(s) 1022, 1254
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 5 cut(s) 335, 742, 1018, 1224, 1588
BccI CCATC 7 cut(s) 113, 148, 257, 341, 648, 1304, 1648
BcgI CGANNNNNNTGC 2 cut(s) 732, 766
BciT130I CCWGG 1 cut(s) 1524
BciVI GTATCC 1 cut(s) 1919
BclI TGATCA 1 cut(s) 1392
BcnI CCSGG 2 cut(s) 1279, 1351
BcoDI GTCTC 4 cut(s) 866, 1573, 1974, 2221
BfaI CTAG 8 cut(s) 171, 876, 1010, 1370, 1919, 2037, 2483, 2665
BfrI CTTAAG 1 cut(s) 1402
BfuI GTATCC 1 cut(s) 1919
BisI GCNGC 6 cut(s) 324, 756, 1007, 1213, 1602, 2061
BlsI GCNGC 6 cut(s) 325, 757, 1008, 1214, 1603, 2062
BmcAI AGTACT 2 cut(s) 1830, 2078
Bme1390I CCNGG 3 cut(s) 1279, 1351, 1524
Bme18I GGWCC 2 cut(s) 917, 1505
BmeT110I CYCGRG 2 cut(s) 1181, 2358
BmgT120I GGNCC 3 cut(s) 386, 917, 1505
BmiI GGNNCC 2 cut(s) 629, 659
BmrFI CCNGG 3 cut(s) 1279, 1351, 1524
BmsI GCATC 1 cut(s) 758
BmtI GCTAGC 1 cut(s) 2486
BpiI GAAGAC 2 cut(s) 1022, 1254
BplI GAGNNNNNCTC 2 cut(s) 1982, 2014
BpmI CTGGAG 1 cut(s) 1545
Bpu10I CCTNAGC 1 cut(s) 1485
Bpu14I TTCGAA 1 cut(s) 228
BpuEI CTTGAG 4 cut(s) 57, 2423, 2517, 2560
BpuMI CCSGG 2 cut(s) 1279, 1351
BsaAI YACGTR 1 cut(s) 1720
BsaBI GATNNNNATC 2 cut(s) 1115, 2433
BsaJI CCNNGG 3 cut(s) 522, 794, 1350
BsaWI WCCGGW 2 cut(s) 422, 1561
BsaXI ACNNNNNCTCC 4 cut(s) 417, 447, 1021, 1051
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 394, 1529
Bse118I RCCGGY 1 cut(s) 1561
Bse1I ACTGG 2 cut(s) 151, 1163
Bse3DI GCAATG 2 cut(s) 750, 2493
Bse8I GATNNNNATC 2 cut(s) 1115, 2433
BseBI CCWGG 1 cut(s) 1524
BseDI CCNNGG 3 cut(s) 522, 794, 1350
BseGI GGATG 6 cut(s) 1296, 1360, 1537, 1676, 2096, 2149
BseJI GATNNNNATC 2 cut(s) 1115, 2433
BseLI CCNNNNNNNGG 3 cut(s) 146, 394, 1529
BseMI GCAATG 2 cut(s) 750, 2493
BseMII CTCAG 3 cut(s) 207, 1353, 2013
BseNI ACTGG 2 cut(s) 151, 1163
BseRI GAGGAG 2 cut(s) 572, 1029
BseXI GCAGC 5 cut(s) 335, 742, 1018, 1224, 1588
BsgI GTGCAG 1 cut(s) 1231
Bsh1236I CGCG 1 cut(s) 2343
Bsh1285I CGRYCG 1 cut(s) 649
BshFI GGCC 4 cut(s) 198, 387, 1854, 2060
BshNI GGYRCC 1 cut(s) 627
BshTI ACCGGT 1 cut(s) 1561
BsiEI CGRYCG 1 cut(s) 649
BsiHKAI GWGCWC 1 cut(s) 575
BsiHKCI CYCGRG 2 cut(s) 1181, 2358
BsiSI CCGG 4 cut(s) 423, 1278, 1350, 1562
BslFI GGGAC 3 cut(s) 347, 793, 1163
BslI CCNNNNNNNGG 3 cut(s) 146, 394, 1529
BsmAI GTCTC 4 cut(s) 866, 1573, 1974, 2221
BsmFI GGGAC 3 cut(s) 347, 793, 1163
BsnI GGCC 4 cut(s) 198, 387, 1854, 2060
BsoBI CYCGRG 2 cut(s) 1181, 2358
Bsp119I TTCGAA 1 cut(s) 228
Bsp1286I GDGCHC 2 cut(s) 575, 959
Bsp19I CCATGG 1 cut(s) 794
BspACI CCGC 5 cut(s) 271, 1422, 2061, 2127, 2353
BspANI GGCC 4 cut(s) 198, 387, 1854, 2060
BspCNI CTCAG 3 cut(s) 208, 1354, 2012
BspFNI CGCG 1 cut(s) 2343
BspLI GGNNCC 2 cut(s) 629, 659
BspOI GCTAGC 1 cut(s) 2486
BspPI GGATC 7 cut(s) 71, 130, 1120, 1163, 1289, 2246, 2423
BspT104I TTCGAA 1 cut(s) 228
BspT107I GGYRCC 1 cut(s) 627
BspTI CTTAAG 1 cut(s) 1402
BsrBI CCGCTC 2 cut(s) 271, 2353
BsrDI GCAATG 2 cut(s) 750, 2493
BsrFI RCCGGY 1 cut(s) 1561
BsrI ACTGG 2 cut(s) 151, 1163
BssAI RCCGGY 1 cut(s) 1561
BssECI CCNNGG 3 cut(s) 522, 794, 1350
BssT1I CCWWGG 2 cut(s) 522, 794
Bst2UI CCWGG 1 cut(s) 1524
Bst4CI ACNGT 9 cut(s) 46, 278, 361, 406, 627, 650, 822, 1069, 2224
Bst6I CTCTTC 2 cut(s) 1038, 2282
BstAFI CTTAAG 1 cut(s) 1402
BstAPI GCANNNNNTGC 1 cut(s) 755
BstBAI YACGTR 1 cut(s) 1720
BstBI TTCGAA 1 cut(s) 228
BstC8I GCNNGC 2 cut(s) 2484, 2662
BstDEI CTNAG 7 cut(s) 216, 248, 802, 1362, 1485, 1625, 1999
BstDSI CCRYGG 1 cut(s) 794
BstEII GGTNACC 1 cut(s) 2243
BstENI CCTNNNNNAGG 1 cut(s) 1527
BstF5I GGATG 6 cut(s) 1296, 1360, 1537, 1676, 2096, 2149
BstFNI CGCG 1 cut(s) 2343
BstHHI GCGC 1 cut(s) 2343
BstMAI GTCTC 4 cut(s) 866, 1573, 1974, 2221
BstMCI CGRYCG 1 cut(s) 649
BstMWI GCNNNNNNNGC 4 cut(s) 755, 1586, 2057, 2165
BstNI CCWGG 1 cut(s) 1524
BstNSI RCATGY 3 cut(s) 62, 457, 984
BstPI GGTNACC 1 cut(s) 2243
BstSCI CCNGG 3 cut(s) 1277, 1349, 1522
BstUI CGCG 1 cut(s) 2343
BstV1I GCAGC 5 cut(s) 335, 742, 1018, 1224, 1588
BstV2I GAAGAC 2 cut(s) 1022, 1254
BstX2I RGATCY 5 cut(s) 76, 122, 1125, 1168, 2428
BstYI RGATCY 5 cut(s) 76, 122, 1125, 1168, 2428
BsuI GTATCC 1 cut(s) 1919
BsuRI GGCC 4 cut(s) 198, 387, 1854, 2060
BtgI CCRYGG 1 cut(s) 794
BtsCI GGATG 6 cut(s) 1296, 1360, 1537, 1676, 2096, 2149
BtsI GCAGTG 1 cut(s) 2058
BtsIMutI CAGTG 2 cut(s) 411, 2058
Cac8I GCNNGC 2 cut(s) 2484, 2662
CfoI GCGC 1 cut(s) 2343
Cfr10I RCCGGY 1 cut(s) 1561
Cfr13I GGNCC 3 cut(s) 386, 917, 1505
Csp6I GTAC 4 cut(s) 628, 1559, 1829, 2077
CspAI ACCGGT 1 cut(s) 1561
CviQI GTAC 4 cut(s) 628, 1559, 1829, 2077
DdeI CTNAG 7 cut(s) 216, 248, 802, 1362, 1485, 1625, 1999
DraIII CACNNNGTG 1 cut(s) 740
DrdI GACNNNNNNGTC 2 cut(s) 924, 1024
DseDI GACNNNNNNGTC 2 cut(s) 924, 1024
EaeI YGGCCR 2 cut(s) 196, 1852
Eam1104I CTCTTC 2 cut(s) 1038, 2282
EarI CTCTTC 2 cut(s) 1038, 2282
Ecl136II GAGCTC 1 cut(s) 573
Eco130I CCWWGG 2 cut(s) 522, 794
Eco24I GRGCYC 2 cut(s) 575, 959
Eco32I GATATC 1 cut(s) 516
Eco47I GGWCC 2 cut(s) 917, 1505
Eco53kI GAGCTC 1 cut(s) 573
Eco57I CTGAAG 2 cut(s) 1481, 2493
Eco88I CYCGRG 2 cut(s) 1181, 2358
Eco91I GGTNACC 1 cut(s) 2243
EcoICRI GAGCTC 1 cut(s) 573
EcoNI CCTNNNNNAGG 1 cut(s) 1527
EcoO65I GGTNACC 1 cut(s) 2243
EcoRI GAATTC 1 cut(s) 881
EcoRII CCWGG 1 cut(s) 1522
EcoRV GATATC 1 cut(s) 516
EcoT14I CCWWGG 2 cut(s) 522, 794
EcoT38I GRGCYC 2 cut(s) 575, 959
ErhI CCWWGG 2 cut(s) 522, 794
FalI AAGNNNNNCTT 4 cut(s) 535, 567, 2148, 2180
FaqI GGGAC 3 cut(s) 347, 793, 1163
FbaI TGATCA 1 cut(s) 1392
FblI GTMKAC 2 cut(s) 1959, 2095
Fnu4HI GCNGC 6 cut(s) 324, 756, 1007, 1213, 1602, 2061
FokI GGATG 6 cut(s) 1283, 1367, 1524, 1663, 2103, 2156
FriOI GRGCYC 2 cut(s) 575, 959
Fsp4HI GCNGC 6 cut(s) 324, 756, 1007, 1213, 1602, 2061
FspBI CTAG 8 cut(s) 171, 876, 1010, 1370, 1919, 2037, 2483, 2665
GlaI GCGC 1 cut(s) 2342
GluI GCNGC 6 cut(s) 324, 756, 1007, 1213, 1602, 2061
GsuI CTGGAG 1 cut(s) 1545
HaeIII GGCC 4 cut(s) 198, 387, 1854, 2060
HapII CCGG 4 cut(s) 423, 1278, 1350, 1562
HhaI GCGC 1 cut(s) 2343
Hin6I GCGC 1 cut(s) 2341
HinP1I GCGC 1 cut(s) 2341
HincII GTYRAC 5 cut(s) 465, 643, 1029, 1427, 2280
HindII GTYRAC 5 cut(s) 465, 643, 1029, 1427, 2280
HindIII AAGCTT 3 cut(s) 234, 1439, 1487
HpaII CCGG 4 cut(s) 423, 1278, 1350, 1562
HphI GGTGA 4 cut(s) 409, 1281, 2024, 2398
Hpy188I TCNGA 6 cut(s) 166, 217, 1090, 1681, 2458, 2595
HpyAV CCTTC 6 cut(s) 845, 1302, 1469, 1523, 1936, 2195
HpyCH4III ACNGT 9 cut(s) 46, 278, 361, 406, 627, 650, 822, 1069, 2224
HpyCH4IV ACGT 1 cut(s) 1719
HpyCH4V TGCA 9 cut(s) 419, 743, 758, 1006, 1212, 1497, 1604, 2083, 2231
HpyF10VI GCNNNNNNNGC 4 cut(s) 755, 1586, 2057, 2165
HpyF3I CTNAG 7 cut(s) 216, 248, 802, 1362, 1485, 1625, 1999
HpySE526I ACGT 1 cut(s) 1719
HspAI GCGC 1 cut(s) 2341
KpnI GGTACC 1 cut(s) 631
Ksp22I TGATCA 1 cut(s) 1392
LmnI GCTCC 6 cut(s) 526, 570, 578, 585, 798, 2479
Lsp1109I GCAGC 5 cut(s) 335, 742, 1018, 1224, 1588
LweI GCATC 1 cut(s) 758
MaeI CTAG 8 cut(s) 171, 876, 1010, 1370, 1919, 2037, 2483, 2665
MaeII ACGT 1 cut(s) 1719
MbiI CCGCTC 2 cut(s) 271, 2353
MboII GAAGA 6 cut(s) 1025, 1027, 1135, 1254, 2299, 2499
MfeI CAATTG 1 cut(s) 2103
MflI RGATCY 5 cut(s) 76, 122, 1125, 1168, 2428
MhlI GDGCHC 2 cut(s) 575, 959
MlsI TGGCCA 1 cut(s) 1854
MluNI TGGCCA 1 cut(s) 1854
MlyI GAGTC 4 cut(s) 206, 210, 2350, 2524
MmeI TCCRAC 3 cut(s) 1153, 1305, 1903
Mox20I TGGCCA 1 cut(s) 1854
MscI TGGCCA 1 cut(s) 1854
MslI CAYNNNNRTG 3 cut(s) 979, 1794, 2115
Msp20I TGGCCA 1 cut(s) 1854
MspCI CTTAAG 1 cut(s) 1402
MspI CCGG 4 cut(s) 423, 1278, 1350, 1562
MspR9I CCNGG 3 cut(s) 1279, 1351, 1524
MunI CAATTG 1 cut(s) 2103
MvaI CCWGG 1 cut(s) 1524
MvnI CGCG 1 cut(s) 2343
MwoI GCNNNNNNNGC 4 cut(s) 755, 1586, 2057, 2165
NciI CCSGG 2 cut(s) 1279, 1351
NcoI CCATGG 1 cut(s) 794
NheI GCTAGC 1 cut(s) 2482
NlaIV GGNNCC 2 cut(s) 629, 659
NmeAIII GCCGAG 1 cut(s) 224
NmuCI GTSAC 3 cut(s) 292, 397, 2623
NspI RCATGY 3 cut(s) 62, 457, 984
NspV TTCGAA 1 cut(s) 228
PaeR7I CTCGAG 1 cut(s) 2358
PciI ACATGT 3 cut(s) 58, 453, 980
PfeI GAWTC 7 cut(s) 87, 167, 608, 809, 968, 2459, 2504
PflMI CCANNNNNTGG 1 cut(s) 146
PinAI ACCGGT 1 cut(s) 1561
PkrI GCNGC 6 cut(s) 325, 757, 1008, 1214, 1603, 2062
PleI GAGTC 4 cut(s) 206, 209, 2350, 2524
PpsI GAGTC 4 cut(s) 206, 209, 2350, 2524
Ppu21I YACGTR 1 cut(s) 1720
PscI ACATGT 3 cut(s) 58, 453, 980
PshBI ATTAAT 1 cut(s) 1221
PsiI TTATAA 1 cut(s) 282
Psp124BI GAGCTC 1 cut(s) 575
Psp6I CCWGG 1 cut(s) 1522
PspEI GGTNACC 1 cut(s) 2243
PspGI CCWGG 1 cut(s) 1522
PspN4I GGNNCC 2 cut(s) 629, 659
PspPI GGNCC 3 cut(s) 386, 917, 1505
PsuI RGATCY 5 cut(s) 76, 122, 1125, 1168, 2428
RsaI GTAC 4 cut(s) 629, 1560, 1830, 2078
RsaNI GTAC 4 cut(s) 628, 1559, 1829, 2077
RseI CAYNNNNRTG 3 cut(s) 979, 1794, 2115
SacI GAGCTC 1 cut(s) 575
SatI GCNGC 6 cut(s) 324, 756, 1007, 1213, 1602, 2061
Sau96I GGNCC 3 cut(s) 386, 917, 1505
ScaI AGTACT 2 cut(s) 1830, 2078
SchI GAGTC 4 cut(s) 206, 210, 2350, 2524
ScrFI CCNGG 3 cut(s) 1279, 1351, 1524
SduI GDGCHC 2 cut(s) 575, 959
SfaNI GCATC 1 cut(s) 758
Sfr274I CTCGAG 1 cut(s) 2358
SfuI TTCGAA 1 cut(s) 228
SinI GGWCC 2 cut(s) 917, 1505
SlaI CTCGAG 1 cut(s) 2358
SmiMI CAYNNNNRTG 3 cut(s) 979, 1794, 2115
SmlI CTYRAG 6 cut(s) 72, 1402, 2358, 2438, 2532, 2575
SmoI CTYRAG 6 cut(s) 72, 1402, 2358, 2438, 2532, 2575
SsiI CCGC 5 cut(s) 271, 1422, 2061, 2127, 2353
SspI AATATT 2 cut(s) 1219, 2261
SspMI CTAG 8 cut(s) 171, 876, 1010, 1370, 1919, 2037, 2483, 2665
SstI GAGCTC 1 cut(s) 575
StyD4I CCNGG 3 cut(s) 1277, 1349, 1522
StyI CCWWGG 2 cut(s) 522, 794
TaaI ACNGT 9 cut(s) 46, 278, 361, 406, 627, 650, 822, 1069, 2224
TaiI ACGT 1 cut(s) 1722
TaqI TCGA 6 cut(s) 180, 228, 971, 2183, 2219, 2359
TaqII GACCGA 1 cut(s) 2536
TatI WGTACW 2 cut(s) 1828, 2076
TauI GCSGC 1 cut(s) 2063
TfiI GAWTC 7 cut(s) 87, 167, 608, 809, 968, 2459, 2504
TscAI CASTG 2 cut(s) 411, 2058
TseFI GTSAC 3 cut(s) 292, 397, 2623
TseI GCWGC 5 cut(s) 323, 755, 1006, 1212, 1601
Tsp45I GTSAC 3 cut(s) 292, 397, 2623
TspDTI ATGAA 8 cut(s) 5, 25, 79, 166, 1119, 1886, 2099, 2160
TspGWI ACGGA 1 cut(s) 99
TspRI CASTG 2 cut(s) 411, 2058
Van91I CCANNNNNTGG 1 cut(s) 146
Vha464I CTTAAG 1 cut(s) 1402
VpaK11BI GGWCC 2 cut(s) 917, 1505
VspI ATTAAT 1 cut(s) 1221
XagI CCTNNNNNAGG 1 cut(s) 1527
XapI RAATTY 6 cut(s) 409, 788, 881, 1303, 1906, 2389
XceI RCATGY 3 cut(s) 62, 457, 984
XcmI CCANNNNNNNNNTGG 1 cut(s) 1136
XhoI CTCGAG 1 cut(s) 2358
XmiI GTMKAC 2 cut(s) 1959, 2095
XspI CTAG 8 cut(s) 171, 876, 1010, 1370, 1919, 2037, 2483, 2665
ZrmI AGTACT 2 cut(s) 1830, 2078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.