Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0021944

Physical Location & Seq

Genomic Coordinates Forward (+)

24234280 .. 24235912

Gene Structure 1633 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G65250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1119 bp

ATGGGTTGGCTGAGGAAGAAGAAGAAGCCAAAATCCGATATAGCGTCCGAGAGAGGGGCCAAGTTGTTAGAAGAGCTCATCGAATGTTGCGATGGCAAATCCAATCCCATCAAATTCTTCTCTGCTGATGAGATCCTCAAAGCCACCAACGATTTCAGCGACTCTAATTTTGTTTTACGATTAGAAGTTCCCTTCAAATGGTATTCTGGTAAGAACGAGAATCATCCCATGATACTAATCAAGAAAGATGTTGGCTGGTGGAGTGGCTTGAGAGTAGATCGTTTGTGCCGCGACATAGCAGTTTCATCGATGGTGAGTGGTCACAAAAACTTCATGAAGTTGGTTGGATGTTGTCTTGAGTTAGATTATCCAGTCATGGTGTATCATAGTGTTAAGAAACATTACAAATTAGAAATAAGTGAACAGCCATGGAAAAAGAGAATGAAGATAGCAGAAGATATCGCTACTGCTTTAGCTTACCTTCACACCGCCTTCCCTAGGCCCTTTGTATATAGGATTTTGTCTCATTGGAATATCTTATTGGATGAAGATGGTGTCGCCAAGCTGACTGATTTCTCTCACTGCGTCTCAATCCCAGAAGGAGAAACATTTGTCCGGGTTGACAGAGACGTGGGATTATATAGTTACTTTGCTGATAATTACGTTAGGAGTGGCCTAGTCTCAGATAAAACAGATGTCTTTGCCTTTGGAATCTTTATGGGTCATAGGCTTCTATTAGGATACGAATACTATTTCGAGCATTATCGAGGAGAAGAAGAAGAAAGTGAAGATGGATTTGACAGTCTGATGAAAAGACATGCTCGGAATTTGCTCTCAACATTAAAGGAAGACAGACCAATGGAAGAGATTGCAGATTCAAAGATGATAGAAAAGATGGGTCAAATTTCAGAACAAGAGCGTTGTCAAATGAAAGCTTTCTTGAAGCTATCACTGAGATGCACCGGCCCTAGTGAAGAAGTTCCAACGATGGTGGAAGTGGCCAAAGAACTAAACAAGATTCAAAGATCTCTTTTTAACGACTCTTCTTCTCTTTCTTCAGGTCAAACCCAACTCGACTCTGCCCAAGACATTTCTTCCACAGTGGTCCTCTCAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

372

Amino Acids

42.73

Weight (kDa)

6.11

Isoelectric Point (pI)

45.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	92 - 247	3.2e-13	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	93 - 315	1.4e-15	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0021944)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65190 AT1G65250 AT1G67470 AT3G57640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	291
AciI	CCGC	2 cut(s)	289, 489
AclWI	GGATC	1 cut(s)	127
AcoI	YGGCCR	1 cut(s)	999
AcsI	RAATTY	3 cut(s)	113, 826, 903
AcuI	CTGAAG	1 cut(s)	1041
AfiI	CCNNNNNNNGG	3 cut(s)	54, 198, 498
AgsI	TTSAA	4 cut(s)	196, 879, 943, 1022
AjiI	CACGTC	1 cut(s)	631
AjuI	GAANNNNNNNTTGG	2 cut(s)	524, 556
AluBI	AGCT	5 cut(s)	76, 476, 565, 935, 946
AluI	AGCT	5 cut(s)	76, 476, 565, 935, 946
Alw21I	GWGCWC	1 cut(s)	78
Alw26I	GTCTC	4 cut(s)	528, 592, 621, 685
AlwI	GGATC	1 cut(s)	127
AoxI	GGCC	5 cut(s)	57, 500, 673, 964, 999
ApoI	RAATTY	3 cut(s)	113, 826, 903
ArsI	GACNNNNNNTTYG	2 cut(s)	152, 184
Asp700I	GAANNNNTTC	2 cut(s)	935, 978
AspA2I	CCTAGG	1 cut(s)	497
AspS9I	GGNCC	4 cut(s)	57, 501, 965, 1105
AsuC2I	CCSGG	1 cut(s)	617
AsuHPI	GGTGA	1 cut(s)	325
AvaII	GGWCC	1 cut(s)	1105
AvrII	CCTAGG	1 cut(s)	497
BalI	TGGCCA	1 cut(s)	1001
BanII	GRGCYC	1 cut(s)	78
BbsI	GAAGAC	1 cut(s)	855
Bbv12I	GWGCWC	1 cut(s)	78
BbvCI	CCTCAGC	1 cut(s)	11
BccI	CCATC	7 cut(s)	86, 116, 304, 545, 785, 889, 982
BcgI	CGANNNNNNTGC	2 cut(s)	288, 322
BciVI	GTATCC	1 cut(s)	734
BcnI	CCSGG	1 cut(s)	617
BcoDI	GTCTC	4 cut(s)	528, 592, 621, 685
BfaI	CTAG	3 cut(s)	498, 677, 969
BfuI	GTATCC	1 cut(s)	734
BglII	AGATCT	1 cut(s)	1025
BisI	GCNGC	1 cut(s)	289
BlnI	CCTAGG	1 cut(s)	497
BlsI	GCNGC	1 cut(s)	290
Bme1390I	CCNGG	1 cut(s)	617
Bme18I	GGWCC	1 cut(s)	1105
BmgBI	CACGTC	1 cut(s)	631
BmgT120I	GGNCC	4 cut(s)	57, 501, 965, 1105
BmiI	GGNNCC	1 cut(s)	58
BmrFI	CCNGG	1 cut(s)	617
BmsI	GCATC	1 cut(s)	947
BpiI	GAAGAC	1 cut(s)	855
Bpu10I	CCTNAGC	1 cut(s)	11
BpuEI	CTTGAG	2 cut(s)	289, 377
BpuMI	CCSGG	1 cut(s)	617
Bsa29I	ATCGAT	1 cut(s)	308
BsaBI	GATNNNNATC	1 cut(s)	236
BsaJI	CCNNGG	2 cut(s)	428, 497
Bsc4I	CCNNNNNNNGG	3 cut(s)	54, 198, 498
Bse118I	RCCGGY	1 cut(s)	962
Bse1I	ACTGG	1 cut(s)	371
Bse8I	GATNNNNATC	1 cut(s)	236
BseCI	ATCGAT	1 cut(s)	308
BseDI	CCNNGG	2 cut(s)	428, 497
BseGI	GGATG	3 cut(s)	223, 353, 550
BseJI	GATNNNNATC	1 cut(s)	236
BseLI	CCNNNNNNNGG	3 cut(s)	54, 198, 498
BseMII	CTCAG	2 cut(s)	696, 944
BseNI	ACTGG	1 cut(s)	371
BseRI	GAGGAG	1 cut(s)	783
Bsh1236I	CGCG	1 cut(s)	291
BshFI	GGCC	5 cut(s)	59, 502, 675, 966, 1001
BshVI	ATCGAT	1 cut(s)	308
BsiHKAI	GWGCWC	1 cut(s)	78
BsiSI	CCGG	2 cut(s)	616, 963
BslI	CCNNNNNNNGG	3 cut(s)	54, 198, 498
BsmAI	GTCTC	4 cut(s)	528, 592, 621, 685
BsmBI	CGTCTC	2 cut(s)	592, 621
BsnI	GGCC	5 cut(s)	59, 502, 675, 966, 1001
Bsp1286I	GDGCHC	1 cut(s)	78
Bsp143I	GATC	3 cut(s)	132, 277, 1025
Bsp19I	CCATGG	1 cut(s)	428
BspACI	CCGC	2 cut(s)	289, 489
BspANI	GGCC	5 cut(s)	59, 502, 675, 966, 1001
BspCNI	CTCAG	2 cut(s)	695, 945
BspDI	ATCGAT	1 cut(s)	308
BspFNI	CGCG	1 cut(s)	291
BspHI	TCATGA	1 cut(s)	333
BspLI	GGNNCC	1 cut(s)	58
BspPI	GGATC	1 cut(s)	127
BspQI	GCTCTTC	1 cut(s)	66
BsrFI	RCCGGY	1 cut(s)	962
BsrI	ACTGG	1 cut(s)	371
BssAI	RCCGGY	1 cut(s)	962
BssECI	CCNNGG	2 cut(s)	428, 497
BssMI	GATC	3 cut(s)	132, 277, 1025
BssT1I	CCWWGG	2 cut(s)	428, 497
Bst4CI	ACNGT	2 cut(s)	803, 1102
Bst6I	CTCTTC	3 cut(s)	66, 858, 1048
BstDEI	CTNAG	3 cut(s)	11, 682, 953
BstDSI	CCRYGG	1 cut(s)	428
BstENI	CCTNNNNNAGG	1 cut(s)	496
BstF5I	GGATG	3 cut(s)	223, 353, 550
BstFNI	CGCG	1 cut(s)	291
BstKTI	GATC	3 cut(s)	135, 280, 1028
BstMAI	GTCTC	4 cut(s)	528, 592, 621, 685
BstMBI	GATC	3 cut(s)	132, 277, 1025
BstNSI	RCATGY	1 cut(s)	821
BstSCI	CCNGG	1 cut(s)	615
BstUI	CGCG	1 cut(s)	291
BstV2I	GAAGAC	1 cut(s)	855
BstX2I	RGATCY	2 cut(s)	132, 1025
BstYI	RGATCY	2 cut(s)	132, 1025
Bsu15I	ATCGAT	1 cut(s)	308
BsuI	GTATCC	1 cut(s)	734
BsuRI	GGCC	5 cut(s)	59, 502, 675, 966, 1001
BsuTUI	ATCGAT	1 cut(s)	308
BtgI	CCRYGG	1 cut(s)	428
BtgZI	GCGATG	1 cut(s)	105
BtrI	CACGTC	1 cut(s)	631
BtsCI	GGATG	3 cut(s)	223, 353, 550
BtsI	GCAGTG	1 cut(s)	580
BtsIMutI	CAGTG	3 cut(s)	580, 950, 1107
CciI	TCATGA	1 cut(s)	333
Cfr10I	RCCGGY	1 cut(s)	962
Cfr13I	GGNCC	4 cut(s)	57, 501, 965, 1105
ClaI	ATCGAT	1 cut(s)	308
CseI	GACGC	2 cut(s)	33, 574
CspCI	CAANNNNNGTGG	2 cut(s)	972, 1007
CviAII	CATG	5 cut(s)	229, 334, 376, 429, 818
DdeI	CTNAG	3 cut(s)	11, 682, 953
DpnI	GATC	3 cut(s)	134, 279, 1027
DpnII	GATC	3 cut(s)	132, 277, 1025
EaeI	YGGCCR	1 cut(s)	999
Eam1104I	CTCTTC	3 cut(s)	66, 858, 1048
EarI	CTCTTC	3 cut(s)	66, 858, 1048
Ecl136II	GAGCTC	1 cut(s)	76
Eco130I	CCWWGG	2 cut(s)	428, 497
Eco24I	GRGCYC	1 cut(s)	78
Eco32I	GATATC	1 cut(s)	460
Eco47I	GGWCC	1 cut(s)	1105
Eco53kI	GAGCTC	1 cut(s)	76
Eco57I	CTGAAG	1 cut(s)	1041
EcoICRI	GAGCTC	1 cut(s)	76
EcoNI	CCTNNNNNAGG	1 cut(s)	496
EcoO109I	RGGNCCY	1 cut(s)	501
EcoRV	GATATC	1 cut(s)	460
EcoT14I	CCWWGG	2 cut(s)	428, 497
EcoT38I	GRGCYC	1 cut(s)	78
ErhI	CCWWGG	2 cut(s)	428, 497
Esp3I	CGTCTC	2 cut(s)	592, 621
FaeI	CATG	5 cut(s)	232, 337, 379, 432, 821
FatI	CATG	5 cut(s)	228, 333, 375, 428, 817
Fnu4HI	GCNGC	1 cut(s)	289
FokI	GGATG	3 cut(s)	210, 360, 557
FriOI	GRGCYC	1 cut(s)	78
Fsp4HI	GCNGC	1 cut(s)	289
FspBI	CTAG	3 cut(s)	498, 677, 969
GluI	GCNGC	1 cut(s)	289
HaeIII	GGCC	5 cut(s)	59, 502, 675, 966, 1001
HapII	CCGG	2 cut(s)	616, 963
HgaI	GACGC	2 cut(s)	33, 574
Hin1II	CATG	5 cut(s)	232, 337, 379, 432, 821
HincII	GTYRAC	1 cut(s)	622
HindII	GTYRAC	1 cut(s)	622
HindIII	AAGCTT	1 cut(s)	933
HinfI	GANTC	7 cut(s)	161, 220, 711, 875, 1018, 1040, 1076
HpaII	CCGG	2 cut(s)	616, 963
HphI	GGTGA	1 cut(s)	325
Hpy166II	GTNNAC	2 cut(s)	422, 622
Hpy188I	TCNGA	6 cut(s)	37, 49, 685, 807, 825, 910
Hpy188III	TCNNGA	4 cut(s)	241, 334, 356, 940
Hpy8I	GTNNAC	2 cut(s)	422, 622
HpyAV	CCTTC	4 cut(s)	202, 491, 502, 593
HpyCH4III	ACNGT	2 cut(s)	803, 1102
HpyCH4IV	ACGT	2 cut(s)	630, 663
HpyCH4V	TGCA	2 cut(s)	872, 960
HpyF3I	CTNAG	3 cut(s)	11, 682, 953
HpySE526I	ACGT	2 cut(s)	630, 663
Hsp92II	CATG	5 cut(s)	232, 337, 379, 432, 821
Kzo9I	GATC	3 cut(s)	132, 277, 1025
LguI	GCTCTTC	1 cut(s)	66
LpnPI	CCDG	7 cut(s)	192, 241, 384, 609, 629, 976, 1044
LweI	GCATC	1 cut(s)	947
MaeI	CTAG	3 cut(s)	498, 677, 969
MaeII	ACGT	2 cut(s)	630, 663
MaeIII	GTNAC	2 cut(s)	320, 644
MalI	GATC	3 cut(s)	134, 279, 1027
MboI	GATC	3 cut(s)	132, 277, 1025
MflI	RGATCY	2 cut(s)	132, 1025
MhlI	GDGCHC	1 cut(s)	78
MlsI	TGGCCA	1 cut(s)	1001
MluCI	AATT	7 cut(s)	113, 166, 407, 658, 826, 903, 1114
MluNI	TGGCCA	1 cut(s)	1001
MlyI	GAGTC	3 cut(s)	155, 1034, 1070
MmeI	TCCRAC	2 cut(s)	325, 1007
MnlI	CCTC	5 cut(s)	6, 47, 146, 761, 1118
Mox20I	TGGCCA	1 cut(s)	1001
MroXI	GAANNNNTTC	2 cut(s)	935, 978
MscI	TGGCCA	1 cut(s)	1001
MseI	TTAA	3 cut(s)	393, 842, 1035
MslI	CAYNNNNRTG	1 cut(s)	955
Msp20I	TGGCCA	1 cut(s)	1001
MspI	CCGG	2 cut(s)	616, 963
MspR9I	CCNGG	1 cut(s)	617
MvnI	CGCG	1 cut(s)	291
NciI	CCSGG	1 cut(s)	617
NcoI	CCATGG	1 cut(s)	428
NdeII	GATC	3 cut(s)	132, 277, 1025
NlaIII	CATG	5 cut(s)	232, 337, 379, 432, 821
NlaIV	GGNNCC	1 cut(s)	58
NmuCI	GTSAC	1 cut(s)	320
NspI	RCATGY	1 cut(s)	821
PagI	TCATGA	1 cut(s)	333
PciSI	GCTCTTC	1 cut(s)	66
PcsI	WCGNNNNNNNCGW	2 cut(s)	87, 156
PdmI	GAANNNNTTC	2 cut(s)	935, 978
PfeI	GAWTC	4 cut(s)	220, 711, 875, 1018
PkrI	GCNGC	1 cut(s)	290
PleI	GAGTC	3 cut(s)	155, 1034, 1070
PpsI	GAGTC	3 cut(s)	155, 1034, 1070
Psp124BI	GAGCTC	1 cut(s)	78
PspN4I	GGNNCC	1 cut(s)	58
PspPI	GGNCC	4 cut(s)	57, 501, 965, 1105
PsuI	RGATCY	2 cut(s)	132, 1025
RseI	CAYNNNNRTG	1 cut(s)	955
SacI	GAGCTC	1 cut(s)	78
SapI	GCTCTTC	1 cut(s)	66
SaqAI	TTAA	3 cut(s)	393, 842, 1035
SatI	GCNGC	1 cut(s)	289
Sau3AI	GATC	3 cut(s)	132, 277, 1025
Sau96I	GGNCC	4 cut(s)	57, 501, 965, 1105
SchI	GAGTC	3 cut(s)	155, 1034, 1070
ScrFI	CCNGG	1 cut(s)	617
SduI	GDGCHC	1 cut(s)	78
SetI	ASST	9 cut(s)	78, 478, 483, 567, 633, 666, 937, 948, 1063
SfaNI	GCATC	1 cut(s)	947
SinI	GGWCC	1 cut(s)	1105
SmiMI	CAYNNNNRTG	1 cut(s)	955
SmlI	CTYRAG	2 cut(s)	268, 356
SmoI	CTYRAG	2 cut(s)	268, 356
Sse9I	AATT	7 cut(s)	113, 166, 407, 658, 826, 903, 1114
SsiI	CCGC	2 cut(s)	289, 489
SspMI	CTAG	3 cut(s)	498, 677, 969
SstI	GAGCTC	1 cut(s)	78
StyD4I	CCNGG	1 cut(s)	615
StyI	CCWWGG	2 cut(s)	428, 497
TaaI	ACNGT	2 cut(s)	803, 1102
TaiI	ACGT	2 cut(s)	633, 666
TaqI	TCGA	5 cut(s)	81, 308, 756, 766, 1074
TasI	AATT	7 cut(s)	113, 166, 407, 658, 826, 903, 1114
TauI	GCSGC	1 cut(s)	291
TfiI	GAWTC	4 cut(s)	220, 711, 875, 1018
Tru1I	TTAA	3 cut(s)	393, 842, 1035
Tru9I	TTAA	3 cut(s)	393, 842, 1035
TscAI	CASTG	3 cut(s)	587, 957, 1107
TseFI	GTSAC	1 cut(s)	320
Tsp45I	GTSAC	1 cut(s)	320
TspDTI	ATGAA	7 cut(s)	294, 322, 350, 458, 561, 824, 944
TspRI	CASTG	3 cut(s)	587, 957, 1107
VpaK11BI	GGWCC	1 cut(s)	1105
XagI	CCTNNNNNAGG	1 cut(s)	496
XapI	RAATTY	3 cut(s)	113, 826, 903
XceI	RCATGY	1 cut(s)	821
XmaJI	CCTAGG	1 cut(s)	497
XmnI	GAANNNNTTC	2 cut(s)	935, 978
XspI	CTAG	3 cut(s)	498, 677, 969

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.