AT1G65450

Transferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0019624
Physical Location & Seq
Forward (+)
24315722 .. 24319092
3371 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65450.1

Sequence Viewer

Length: 1353 bp
ATGGGTTCTTCGTACCAAGAATCTCCACCGTTGCTTCTTGAAGATCTTAAGGTTACGATCAAAGAATCAACACTCATTTTCCCATCTGAAGAAACCAGCGAGAGAAAATCCATGTTCTTATCCAACGTCGACCAAATTCTCAACTTTGACGTTCAAACAGTTCATTTCTTCCGACCTAACAAGGAGTTTCCACCGGAGATGGTCTCGGAGAAGCTGAGAAAGGCGTTGGTGAAACTCATGGACGCTTATGAGTTCTTGGCCGGGAGACTCCGCGTGGATCCTAGCTCTGGACGGCTTGATGTTGACTGTAACGGTGCTGGAGCTGGCTTTGTGACGGCGGCGAGTGACTACACGTTGGAGGAACTTGGAGATTTGGTTTATCCAAATCCAGCTTTTGCTCAATTGGTTACAAGTCAGCTTCAGAGTTTGCCCAAAGATGATCAACCCTTGTTTGTCTTTCAGATAACATCATTCAAGTGCGGTGGATTTGCAATGGGAATCTCAACAAACCATACCACGTTCGATGGACTTAGTTTCAAAACATTCCTCGAAAACTTAGCTTCTCTACTTCACGAGAAGCCCTTATCAACACCTCCATGCAACGACCGTACTCTTCTCAAAGCTCGTGATCCACCAAGTGTTGCATTCCCACACCACGAGCTTGTCAAGTTCCAAGACTGTGAGACAACAACAGTCTTCGAAGCTACTTCAGAGCACTTAGACTTCAAGATCTTCAAGCTATCCTCTGAACAAATCAAGAAGCTCAAGGAGAGAGCCTCAGAGACTAGTAACGGTAATGTTCGCGTGACGGGTTTCAACGTTGTAACCGCTTTGGTCTGGAGGTGCAAGGCACTCTCTGTAGCAGCAGAGGAAGGAGAAGAAACCAATCTTGAAAGAGAATCAACGATTCTTTATGCTGTGGACATTAGAGGGAGGCTGAATCCTGAGCTTCCACCGTCTTACACTGGAAACGCGGTTTTAACGGCATACGCAAAGGAGAAATGCAAGGCATTGCTTGAAGAGCCGTTTGGGAGGATTGTAGAAATGGTAGGAGAAGGGTCAAAGAGGATAACGGATGAGTATGCGCGGTCTGCTATAGATTGGGGAGAGTTATACAAAGGGTTTCCCCATGGGGAAGTTTTGGTTTCTTCTTGGTGGAAACTCGGATTCGCAGAGGTTGAGTATCCTTGGGGAAAGCCTAAGTATAGCTGTCCCGTTGTCTATCATCGAAAAGACATTGTTTTGCTGTTTCCGGACATCGATGGAGATAGTAAAGGTGTTTATGTCTTGGCTGCTTTGCCTTCTAAGGAGATGTCCAAGTTTCAGCACTGGTTTGAAGACACTCTGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
Transferase

Protein Analysis

450

Amino Acids

50.5

Weight (kDa)

5.22

Isoelectric Point (pI)

30.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 16 - 447 6.6e-155 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019624)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65450 AT1G65450 AT1G65450
fragaria_vesca FvH4_6g00100
prunus_persica Prupe.6G364800_v2.0.a1
rosa_laevigata RLG00000025997
rosa_roxburghii Rroxscaffold_6G00425660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 129
AccII CGCG 4 cut(s) 273, 804, 974, 1087
AccIII TCCGGA 1 cut(s) 1252
AciI CCGC 6 cut(s) 271, 338, 480, 828, 974, 1087
AclI AACGTT 1 cut(s) 819
AclWI GGATC 3 cut(s) 272, 285, 623
AcoI YGGCCR 1 cut(s) 258
AcsI RAATTY 1 cut(s) 135
AcuI CTGAAG 3 cut(s) 108, 404, 693
AdeI CACNNNGTG 2 cut(s) 638, 1347
AfaI GTAC 2 cut(s) 14, 610
AfiI CCNNNNNNNGG 1 cut(s) 287
AflII CTTAAG 1 cut(s) 47
AflIII ACRYGT 1 cut(s) 351
AhlI ACTAGT 1 cut(s) 785
AjuI GAANNNNNNNTTGG 2 cut(s) 1011, 1043
Alw21I GWGCWC 1 cut(s) 717
Alw26I GTCTC 4 cut(s) 208, 259, 677, 776
AlwI GGATC 3 cut(s) 272, 285, 623
Aor13HI TCCGGA 1 cut(s) 1252
AoxI GGCC 1 cut(s) 258
ApeKI GCWGC 2 cut(s) 863, 1292
ApoI RAATTY 1 cut(s) 135
AspLEI GCGC 1 cut(s) 1087
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 1 cut(s) 241
AsuII TTCGAA 1 cut(s) 699
BamHI GGATCC 1 cut(s) 277
BauI CACGAG 3 cut(s) 572, 624, 656
BbsI GAAGAC 2 cut(s) 688, 1344
Bbv12I GWGCWC 1 cut(s) 717
BbvI GCAGC 2 cut(s) 875, 1279
BccI CCATC 4 cut(s) 91, 193, 518, 1256
BceAI ACGGC 4 cut(s) 308, 351, 999, 1009
BciVI GTATCC 1 cut(s) 1194
BclI TGATCA 1 cut(s) 439
BcnI CCSGG 1 cut(s) 262
BcoDI GTCTC 4 cut(s) 208, 259, 677, 776
BcuI ACTAGT 1 cut(s) 785
BfaI CTAG 2 cut(s) 282, 786
BfmI CTRYAG 2 cut(s) 858, 1095
BfrI CTTAAG 1 cut(s) 47
BfuI GTATCC 1 cut(s) 1194
BglII AGATCT 2 cut(s) 43, 729
BisI GCNGC 3 cut(s) 339, 864, 1293
BlsI GCNGC 3 cut(s) 340, 865, 1294
Bme1390I CCNGG 1 cut(s) 262
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 1 cut(s) 262
BpiI GAAGAC 2 cut(s) 688, 1344
BplI GAGNNNNNCTC 4 cut(s) 188, 220, 761, 793
BpmI CTGGAG 2 cut(s) 339, 859
Bpu10I CCTNAGC 1 cut(s) 945
Bpu14I TTCGAA 1 cut(s) 699
BpuEI CTTGAG 1 cut(s) 749
BpuMI CCSGG 1 cut(s) 262
Bsa29I ATCGAT 1 cut(s) 1260
BsaI GGTCTC 1 cut(s) 208
BsaJI CCNNGG 2 cut(s) 1129, 1187
BsaWI WCCGGW 2 cut(s) 193, 1252
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 970, 1334
Bse3DI GCAATG 2 cut(s) 498, 1010
BseAI TCCGGA 1 cut(s) 1252
BseCI ATCGAT 1 cut(s) 1260
BseDI CCNNGG 2 cut(s) 1129, 1187
BseGI GGATG 1 cut(s) 1081
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMI GCAATG 2 cut(s) 498, 1010
BseMII CTCAG 3 cut(s) 206, 792, 936
BseNI ACTGG 2 cut(s) 970, 1334
BseXI GCAGC 2 cut(s) 875, 1279
Bsh1236I CGCG 4 cut(s) 273, 804, 974, 1087
Bsh1285I CGRYCG 1 cut(s) 607
BshFI GGCC 1 cut(s) 260
BshVI ATCGAT 1 cut(s) 1260
BsiEI CGRYCG 1 cut(s) 607
BsiHKAI GWGCWC 1 cut(s) 717
BsiSI CCGG 3 cut(s) 194, 261, 1253
BslFI GGGAC 1 cut(s) 1197
BslI CCNNNNNNNGG 1 cut(s) 287
BsmAI GTCTC 4 cut(s) 208, 259, 677, 776
BsmFI GGGAC 1 cut(s) 1197
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 1 cut(s) 260
Bso31I GGTCTC 1 cut(s) 208
Bsp119I TTCGAA 1 cut(s) 699
Bsp1286I GDGCHC 1 cut(s) 717
Bsp13I TCCGGA 1 cut(s) 1252
Bsp143I GATC 6 cut(s) 43, 57, 277, 439, 628, 729
Bsp19I CCATGG 1 cut(s) 1129
BspACI CCGC 6 cut(s) 271, 338, 480, 828, 974, 1087
BspANI GGCC 1 cut(s) 260
BspCNI CTCAG 3 cut(s) 207, 791, 937
BspDI ATCGAT 1 cut(s) 1260
BspEI TCCGGA 1 cut(s) 1252
BspFNI CGCG 4 cut(s) 273, 804, 974, 1087
BspLI GGNNCC 1 cut(s) 279
BspPI GGATC 3 cut(s) 272, 285, 623
BspQI GCTCTTC 1 cut(s) 1014
BspT104I TTCGAA 1 cut(s) 699
BspTI CTTAAG 1 cut(s) 47
BspTNI GGTCTC 1 cut(s) 208
BsrDI GCAATG 2 cut(s) 498, 1010
BsrI ACTGG 2 cut(s) 970, 1334
BssECI CCNNGG 2 cut(s) 1129, 1187
BssMI GATC 6 cut(s) 43, 57, 277, 439, 628, 729
BssSI CACGAG 3 cut(s) 572, 624, 656
BssT1I CCWWGG 2 cut(s) 1129, 1187
Bst2BI CACGAG 3 cut(s) 572, 624, 656
Bst4CI ACNGT 9 cut(s) 30, 160, 308, 314, 608, 680, 694, 794, 957
Bst6I CTCTTC 2 cut(s) 618, 1014
BstAFI CTTAAG 1 cut(s) 47
BstBI TTCGAA 1 cut(s) 699
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 8 cut(s) 215, 530, 556, 718, 778, 945, 1200, 1305
BstDSI CCRYGG 1 cut(s) 1129
BstF5I GGATG 1 cut(s) 1081
BstFNI CGCG 4 cut(s) 273, 804, 974, 1087
BstHHI GCGC 1 cut(s) 1087
BstKTI GATC 6 cut(s) 46, 60, 280, 442, 631, 732
BstMAI GTCTC 4 cut(s) 208, 259, 677, 776
BstMBI GATC 6 cut(s) 43, 57, 277, 439, 628, 729
BstMCI CGRYCG 1 cut(s) 607
BstMWI GCNNNNNNNGC 2 cut(s) 1021, 1091
BstSCI CCNGG 1 cut(s) 260
BstSFI CTRYAG 2 cut(s) 858, 1095
BstUI CGCG 4 cut(s) 273, 804, 974, 1087
BstV1I GCAGC 2 cut(s) 875, 1279
BstV2I GAAGAC 2 cut(s) 688, 1344
BstX2I RGATCY 3 cut(s) 43, 277, 729
BstYI RGATCY 3 cut(s) 43, 277, 729
Bsu15I ATCGAT 1 cut(s) 1260
BsuI GTATCC 1 cut(s) 1194
BsuRI GGCC 1 cut(s) 260
BsuTUI ATCGAT 1 cut(s) 1260
BtgI CCRYGG 1 cut(s) 1129
BtsCI GGATG 1 cut(s) 1081
BtsIMutI CAGTG 2 cut(s) 963, 1327
Cac8I GCNNGC 1 cut(s) 325
CfoI GCGC 1 cut(s) 1087
ClaI ATCGAT 1 cut(s) 1260
CseI GACGC 1 cut(s) 251
Csp6I GTAC 2 cut(s) 13, 609
CspCI CAANNNNNGTGG 2 cut(s) 463, 498
CviAII CATG 4 cut(s) 112, 238, 597, 1130
CviQI GTAC 2 cut(s) 13, 609
DdeI CTNAG 8 cut(s) 215, 530, 556, 718, 778, 945, 1200, 1305
DpnI GATC 6 cut(s) 45, 59, 279, 441, 630, 731
DpnII GATC 6 cut(s) 43, 57, 277, 439, 628, 729
DraIII CACNNNGTG 2 cut(s) 638, 1347
EaeI YGGCCR 1 cut(s) 258
Eam1104I CTCTTC 2 cut(s) 618, 1014
EarI CTCTTC 2 cut(s) 618, 1014
Eco130I CCWWGG 2 cut(s) 1129, 1187
Eco31I GGTCTC 1 cut(s) 208
Eco57I CTGAAG 3 cut(s) 108, 404, 693
EcoT14I CCWWGG 2 cut(s) 1129, 1187
ErhI CCWWGG 2 cut(s) 1129, 1187
FaeI CATG 4 cut(s) 115, 241, 600, 1133
FaqI GGGAC 1 cut(s) 1197
FatI CATG 4 cut(s) 111, 237, 596, 1129
FbaI TGATCA 1 cut(s) 439
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 3 cut(s) 339, 864, 1293
FokI GGATG 1 cut(s) 1088
Fsp4HI GCNGC 3 cut(s) 339, 864, 1293
FspBI CTAG 2 cut(s) 282, 786
GlaI GCGC 1 cut(s) 1086
GluI GCNGC 3 cut(s) 339, 864, 1293
GsuI CTGGAG 2 cut(s) 339, 859
HaeIII GGCC 1 cut(s) 260
HapII CCGG 3 cut(s) 194, 261, 1253
HgaI GACGC 1 cut(s) 251
HhaI GCGC 1 cut(s) 1087
Hin1II CATG 4 cut(s) 115, 241, 600, 1133
Hin6I GCGC 1 cut(s) 1085
HinP1I GCGC 1 cut(s) 1085
HincII GTYRAC 2 cut(s) 130, 304
HindII GTYRAC 2 cut(s) 130, 304
HinfI GANTC 8 cut(s) 20, 65, 267, 498, 899, 907, 940, 1167
HpaII CCGG 3 cut(s) 194, 261, 1253
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 3 cut(s) 130, 304, 922
Hpy188I TCNGA 9 cut(s) 88, 173, 208, 423, 462, 712, 748, 781, 1166
Hpy8I GTNNAC 3 cut(s) 130, 304, 922
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 3 cut(s) 866, 1049, 1311
HpyCH4III ACNGT 9 cut(s) 30, 160, 308, 314, 608, 680, 694, 794, 957
HpyCH4IV ACGT 5 cut(s) 126, 150, 353, 518, 819
HpyCH4V TGCA 5 cut(s) 491, 600, 644, 846, 1005
HpyF10VI GCNNNNNNNGC 2 cut(s) 1021, 1091
HpyF3I CTNAG 8 cut(s) 215, 530, 556, 718, 778, 945, 1200, 1305
HpySE526I ACGT 5 cut(s) 126, 150, 353, 518, 819
Hsp92II CATG 4 cut(s) 115, 241, 600, 1133
HspAI GCGC 1 cut(s) 1085
Kpn2I TCCGGA 1 cut(s) 1252
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 6 cut(s) 43, 57, 277, 439, 628, 729
LguI GCTCTTC 1 cut(s) 1014
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 2 cut(s) 875, 1279
MaeI CTAG 2 cut(s) 282, 786
MaeII ACGT 5 cut(s) 126, 150, 353, 518, 819
MaeIII GTNAC 8 cut(s) 52, 308, 331, 344, 406, 788, 805, 823
MalI GATC 6 cut(s) 45, 59, 279, 441, 630, 731
MboI GATC 6 cut(s) 43, 57, 277, 439, 628, 729
MfeI CAATTG 1 cut(s) 401
MflI RGATCY 3 cut(s) 43, 277, 729
MhlI GDGCHC 1 cut(s) 717
MluCI AATT 2 cut(s) 135, 401
MlyI GAGTC 1 cut(s) 261
MmeI TCCRAC 3 cut(s) 147, 196, 336
MroI TCCGGA 1 cut(s) 1252
MseI TTAA 2 cut(s) 48, 980
MslI CAYNNNNRTG 2 cut(s) 475, 595
MspCI CTTAAG 1 cut(s) 47
MspI CCGG 3 cut(s) 194, 261, 1253
MspR9I CCNGG 1 cut(s) 262
MunI CAATTG 1 cut(s) 401
Mva1269I GAATGC 1 cut(s) 644
MvnI CGCG 4 cut(s) 273, 804, 974, 1087
MwoI GCNNNNNNNGC 2 cut(s) 1021, 1091
NciI CCSGG 1 cut(s) 262
NcoI CCATGG 1 cut(s) 1129
NdeII GATC 6 cut(s) 43, 57, 277, 439, 628, 729
NlaIII CATG 4 cut(s) 115, 241, 600, 1133
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 3 cut(s) 331, 344, 805
NspV TTCGAA 1 cut(s) 699
PciSI GCTCTTC 1 cut(s) 1014
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 7 cut(s) 20, 65, 498, 899, 907, 940, 1167
PkrI GCNGC 3 cut(s) 340, 865, 1294
PleI GAGTC 1 cut(s) 261
PpsI GAGTC 1 cut(s) 261
Psp1406I AACGTT 1 cut(s) 819
PspN4I GGNNCC 1 cut(s) 279
PsuI RGATCY 3 cut(s) 43, 277, 729
RsaI GTAC 2 cut(s) 14, 610
RsaNI GTAC 2 cut(s) 13, 609
RseI CAYNNNNRTG 2 cut(s) 475, 595
SalI GTCGAC 1 cut(s) 128
SapI GCTCTTC 1 cut(s) 1014
SaqAI TTAA 2 cut(s) 48, 980
SatI GCNGC 3 cut(s) 339, 864, 1293
Sau3AI GATC 6 cut(s) 43, 57, 277, 439, 628, 729
SchI GAGTC 1 cut(s) 261
ScrFI CCNGG 1 cut(s) 262
SduI GDGCHC 1 cut(s) 717
SfcI CTRYAG 2 cut(s) 858, 1095
SfuI TTCGAA 1 cut(s) 699
SmiMI CAYNNNNRTG 2 cut(s) 475, 595
SmlI CTYRAG 2 cut(s) 47, 764
SmoI CTYRAG 2 cut(s) 47, 764
SpeI ACTAGT 1 cut(s) 785
Sse9I AATT 2 cut(s) 135, 401
SsiI CCGC 6 cut(s) 271, 338, 480, 828, 974, 1087
SspMI CTAG 2 cut(s) 282, 786
StyD4I CCNGG 1 cut(s) 260
StyI CCWWGG 2 cut(s) 1129, 1187
TaaI ACNGT 9 cut(s) 30, 160, 308, 314, 608, 680, 694, 794, 957
TaiI ACGT 5 cut(s) 129, 153, 356, 521, 822
TaqI TCGA 6 cut(s) 129, 522, 549, 699, 1228, 1260
TasI AATT 2 cut(s) 135, 401
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 7 cut(s) 20, 65, 498, 899, 907, 940, 1167
Tru1I TTAA 2 cut(s) 48, 980
Tru9I TTAA 2 cut(s) 48, 980
TscAI CASTG 2 cut(s) 970, 1334
TseFI GTSAC 3 cut(s) 331, 344, 805
TseI GCWGC 2 cut(s) 863, 1292
Tsp45I GTSAC 3 cut(s) 331, 344, 805
TspDTI ATGAA 1 cut(s) 152
TspGWI ACGGA 1 cut(s) 1088
TspRI CASTG 2 cut(s) 970, 1334
Vha464I CTTAAG 1 cut(s) 47
XapI RAATTY 1 cut(s) 135
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 2 cut(s) 282, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.