AT1G66410

Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2 ). Among the enzymes to be stimulated by the calmodulin-Ca(2 ) complex are a number of protein kinases and phosphatases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0020827
Physical Location & Seq
Reverse (-)
24774113 .. 24775984
1872 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G66410.3

Sequence Viewer

Length: 450 bp
ATGGCGGATCAGCTAACTGATGAACAGATCTCTGAGTTTAAGGAAGCTTTTAGCCTCTTCGACAAAGATGGAGATGGTTGCATCACCACAAAGGAGCTGGGAACAGTGATGCGGTCACTAGGGCAGAACCCAACAGAAGCTGAGCTACAAGACATGATCAACGAGGTTGACGCTGATGGAAACGGAACCATTGACTTCCCTGAGTTCTTAAACCTAATGGCTAAAAAGATGAAGGACACAGACTCGGAGGAAGAGCTGAAAGAAGCTTTCAGGGTGTTCGACAAAGACCAGAACGGTTTCATATCCGCTGCAGAGCTTCGCCATGTGATGACGAATCTAGGTGAGAAACTGACTGATGAAGAGGTCGAGGAGATGATTCGTGAAGCAGATGTTGATGGAGATGGTCAGATAAACTACGAGGAGTTTGTCAAGATTATGATGGCTAAGTGA

Protein Analysis

149

Amino Acids

16.86

Weight (kDa)

4.14

Isoelectric Point (pI)

29.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 2 - 39 7.2e-08 EF-hand domain pair
EF-hand_7 PF13499 3 - 37 1.4e-07 EF-hand domain pair
EF-hand_1 PF00036 12 - 39 1.4e-08 EF hand domain
EF-hand_7 PF13499 47 - 111 2.1e-22 EF-hand domain pair
AIF-1 PF21008 47 - 113 9.6e-07 Allograft inflammatory factor 1
EF-hand_1 PF00036 49 - 76 1.6e-09 EF hand domain
EF-hand_6 PF13405 49 - 78 1.2e-09 EF-hand domain
EF-hand_5 PF13202 50 - 74 8.5e-07 EF hand
EF-hand_9 PF14658 52 - 112 8.8e-08 EF-hand domain
EF-hand_8 PF13833 65 - 108 1.7e-11 EF-hand domain pair
EF-hand_1 PF00036 85 - 112 2.9e-10 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0020827)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66410 AT1G66410 AT5G37780 AT5G37780
rosa_laevigata RLG00000022862

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 112, 306
AclWI GGATC 1 cut(s) 15
AluBI AGCT 8 cut(s) 13, 47, 97, 140, 145, 256, 266, 316
AluI AGCT 8 cut(s) 13, 47, 97, 140, 145, 256, 266, 316
AlwI GGATC 1 cut(s) 15
AlwNI CAGNNNCTG 1 cut(s) 140
ApeKI GCWGC 1 cut(s) 308
Asp700I GAANNNNTTC 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 76, 353
BarI GAAGNNNNNNTAC 2 cut(s) 129, 161
BbvI GCAGC 1 cut(s) 295
BccI CCATC 6 cut(s) 62, 68, 170, 389, 395, 433
BclI TGATCA 1 cut(s) 156
BfaI CTAG 2 cut(s) 119, 338
BfmI CTRYAG 1 cut(s) 309
BglII AGATCT 1 cut(s) 27
BisI GCNGC 1 cut(s) 309
BlpI GCTNAGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 310
BmiI GGNNCC 1 cut(s) 187
BmsI GCATC 2 cut(s) 90, 99
Bpu1102I GCTNAGC 1 cut(s) 141
BseMII CTCAG 3 cut(s) 24, 132, 192
BseRI GAGGAG 2 cut(s) 383, 434
BseXI GCAGC 1 cut(s) 295
BseYI CCCAGC 1 cut(s) 97
Bsp143I GATC 3 cut(s) 7, 27, 156
Bsp1720I GCTNAGC 1 cut(s) 141
BspACI CCGC 3 cut(s) 5, 112, 306
BspCNI CTCAG 3 cut(s) 25, 133, 193
BspLI GGNNCC 1 cut(s) 187
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 1 cut(s) 15
BspQI GCTCTTC 1 cut(s) 246
BssMI GATC 3 cut(s) 7, 27, 156
Bst4CI ACNGT 2 cut(s) 106, 296
Bst6I CTCTTC 3 cut(s) 62, 246, 354
BstDEI CTNAG 4 cut(s) 33, 141, 201, 444
BstKTI GATC 3 cut(s) 10, 30, 159
BstMBI GATC 3 cut(s) 7, 27, 156
BstSFI CTRYAG 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 295
BstX2I RGATCY 1 cut(s) 27
BstYI RGATCY 1 cut(s) 27
BtsIMutI CAGTG 1 cut(s) 111
CaiI CAGNNNCTG 1 cut(s) 140
CseI GACGC 1 cut(s) 179
CviAII CATG 2 cut(s) 154, 323
DdeI CTNAG 4 cut(s) 33, 141, 201, 444
DpnI GATC 3 cut(s) 9, 29, 158
DpnII GATC 3 cut(s) 7, 27, 156
Eam1104I CTCTTC 3 cut(s) 62, 246, 354
EarI CTCTTC 3 cut(s) 62, 246, 354
EciI GGCGGA 1 cut(s) 20
FaeI CATG 2 cut(s) 157, 326
FaiI YATR 4 cut(s) 155, 302, 324, 437
FatI CATG 2 cut(s) 153, 322
FbaI TGATCA 1 cut(s) 156
Fnu4HI GCNGC 1 cut(s) 309
Fsp4HI GCNGC 1 cut(s) 309
FspBI CTAG 2 cut(s) 119, 338
GluI GCNGC 1 cut(s) 309
GsaI CCCAGC 1 cut(s) 101
HgaI GACGC 1 cut(s) 179
Hin1II CATG 2 cut(s) 157, 326
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 2 cut(s) 45, 264
HinfI GANTC 3 cut(s) 242, 334, 376
HphI GGTGA 2 cut(s) 76, 353
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 3 cut(s) 34, 247, 408
Hpy188III TCNNGA 2 cut(s) 380, 430
Hpy8I GTNNAC 1 cut(s) 169
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 2 cut(s) 106, 296
HpyCH4V TGCA 2 cut(s) 81, 311
HpyF3I CTNAG 4 cut(s) 33, 141, 201, 444
Hsp92II CATG 2 cut(s) 157, 326
Ksp22I TGATCA 1 cut(s) 156
Kzo9I GATC 3 cut(s) 7, 27, 156
LguI GCTCTTC 1 cut(s) 246
LmnI GCTCC 1 cut(s) 94
LpnPI CCDG 4 cut(s) 83, 213, 256, 302
Lsp1109I GCAGC 1 cut(s) 295
LweI GCATC 2 cut(s) 90, 99
MaeI CTAG 2 cut(s) 119, 338
MaeIII GTNAC 1 cut(s) 114
MalI GATC 3 cut(s) 9, 29, 158
MboI GATC 3 cut(s) 7, 27, 156
MboII GAAGA 3 cut(s) 49, 263, 371
MflI RGATCY 1 cut(s) 27
MlyI GAGTC 1 cut(s) 236
MnlI CCTC 6 cut(s) 65, 157, 241, 355, 361, 412
MroXI GAANNNNTTC 1 cut(s) 296
MseI TTAA 2 cut(s) 39, 209
MspA1I CMGCKG 1 cut(s) 308
NdeII GATC 3 cut(s) 7, 27, 156
NlaIII CATG 2 cut(s) 157, 326
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 114
PciSI GCTCTTC 1 cut(s) 246
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 2 cut(s) 334, 376
PkrI GCNGC 1 cut(s) 310
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
PspFI CCCAGC 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 187
PstI CTGCAG 1 cut(s) 313
PstNI CAGNNNCTG 1 cut(s) 140
PsuI RGATCY 1 cut(s) 27
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 2 cut(s) 39, 209
SatI GCNGC 1 cut(s) 309
Sau3AI GATC 3 cut(s) 7, 27, 156
SchI GAGTC 1 cut(s) 236
SfaNI GCATC 2 cut(s) 90, 99
SfcI CTRYAG 1 cut(s) 309
SsiI CCGC 3 cut(s) 5, 112, 306
SspMI CTAG 2 cut(s) 119, 338
TaaI ACNGT 2 cut(s) 106, 296
TaqI TCGA 3 cut(s) 60, 279, 366
TfiI GAWTC 2 cut(s) 334, 376
Tru1I TTAA 2 cut(s) 39, 209
Tru9I TTAA 2 cut(s) 39, 209
TscAI CASTG 1 cut(s) 111
TseFI GTSAC 1 cut(s) 114
TseI GCWGC 1 cut(s) 308
Tsp45I GTSAC 1 cut(s) 114
TspDTI ATGAA 4 cut(s) 36, 245, 289, 372
TspGWI ACGGA 1 cut(s) 198
TspRI CASTG 1 cut(s) 111
XcmI CCANNNNNNNNNTGG 1 cut(s) 94
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 2 cut(s) 119, 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.