Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0021861

Physical Location & Seq

Genomic Coordinates Forward (+)

27446069 .. 27451067

Gene Structure 4999 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G72960.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2388 bp

ATGGATGCAGATAAAAGCGAGGGATGTTGTTCTGTACAGCTCATCGACGGAGATGGTATATACAATGTCTCTCGCATCGATCATTTCATTAAAGATGTGAAACTTGCAGACTGTGGCCTTTCTTATGCTGTTGTCTCTATTATGGGTCCTCAAAGTAGCGGGAAAAGTACACTATTGAACCATTTGTTTGGAACAAACTTTATGGAGATGGATGCATTCAAGGGAAGGTCTCAGACCACAAAAGGTATCTGGCTTGCAAGATGTGCCGGTATAGAGCCTTGCACCCTTGTAATGGATTTAGAGGGCACCGATGGCAGAGAGCGAGGAGAGGATGATACGGCATTCGAAAAACAGAGTGCTCTTTTCGCTCTTGCTATCTCTGATATCGTCTTGATCAACATGTGGTGTCACGACATTGGCCGTGAACAAGCTGCTAATAAACCTCTCTTGAAAACTGTCTTTCAGGTGATGATGCGATTGTTTAGTCCACGTAAAACAACAATGCTGTTTGTAATACGAGATAAAACCCGGACACCGTTGGAAAATTTAGAGCCTGTCTTAAGAGAAGATATTCAGAAGATATGGGATTCTGTTCCAAAGCCTGAAGCACACAAGGAAACTCCTCTAAGTGACTTTTTCAATGTTGAAGTTGTTGCTCTTTCCAGTTATGAGGAGAAGGAAGAACAGTTTAAAGAGCAGATTGCTAGTTTGAGACAACGGTTCATGCATTCTATTGCACCGGGAGGTCTTGCTGGAGACAGAAGAGGAGTGATTCCAGCCTCAGGTTTTGCATTTAGTGCAGATCAGATCTGGAGAGTCATCAAAGAGAATAAGGATCTTGACCTTCCAGCCCACAAGGTCATGGTGGCTACTGTGCGGTGCGAAGAAATTGCAAATGAGAAGTTTGCGCATTTCATTACAAACGAGGACTGGCGCAAACTGGATGAGGAGGTGCAAGCCGGTCCAGTTTCTAATTTTGGAAAGAGGCTTACTACCATTCTCGGTTCTTGCTTATCAGAATATGACGGGGAGGCTACATTCTTCGATGAAGGTGTCAGATCTTCGAAGAGACAGCAGCTTGAAGAAAAGCTTCTCCAACTTGTGAATCCAGCGTTTCAAGATGTATTGGGACATATAAGATGGGGAATACTTGAAAAGTTTAAGGCTTCTTTTGATAAGGCTTTAGGCATTGGTGAAGGGTTTTCTTCAGCTTCCCAAGATTGGTTCAAGGCTTGCATGACCCAATTCGATGAAGAGTGTGCAGGTGCCATCATTGAACAAGCCAATTGGGACACATCAAAAGTACGGGACAAGCTAGTACGTGACATTGAGGCTCACATTTCCTCTGTGCGGACTTCCAAGTTATCTGAACTTACCAGTCTATACGAGTCAAAAGTTCATGAAGCTTTATCAGAACCGGTGGAAGCTTTGTTGGATGGAGCCAACGATGAAACGTGGAGCACAGTAAAGAAGCTTCATCGGCGTGAAACTGAATCTGCAGTATCTGGACTCAGTAGTGCATTAGCTGGTTTTGACATGGAAGAAGAAACAAGAGATAGAATGGTGAAGAGCCTTCAGGATTATGCAAGAGGTGTCATTGAAACGAAGGCAAAAGAAGAGGCTGTGCGGGTTTTGATGCGTATGAAGGAAAGGTTTGGTACAATCTTCAGCCATGATTCTGATTCAATGCCACGTGTTTGGACCGGAAAAGAGGATCTTCGAGCAATTACTAAATCAGCTCGATCTGCTTCCTTGAAGTTGCTCTCAGTAATGGCTGTCATTCGATTGGGGGATGAACCTGATAACATCGAGAAGACACTAACTGTAGCTCTGCTGGATCCAACAAAGAATGATACTTCTAAGAAAAGCATCACAACATCTGATCCACTGGCTTCAAGCACTTGGGATGAGGTTCCATCATCAAGAACTTTGATCACACCGGTGCAATGTAAATCTATATGGAGACAGTTCAAGACAGAAACAGAGTATACAGTGACTCAAGCCATATCCGCACAGGAGGCGAACAGGCGTGGAAATAACTGGTTACCTCCTCCATGGGCAATTCTTGCATTGATCGTCCTTGGATTCAACGAATTCATGACTCTTTTAAGAAACCCTCTCTATCTTGGTGTCATGTTTGTCGCTTTCCTTCTGGCAAAAGCACTGTGGACGCAATTGGATATCCCTGGCGAATTCCGCAATGGTGCACTTCCAGGGCTCATATCGATATCCGCAAAGTTTGTTCCCACAGTAATGAACCTTATCAAGAACCTTGCAGCGCAAGGCGAAGACCCTCCTGCAGCTAATCCAGAGAACCGTCGTTCGAGCAACAACACTTCTTCATCAGAAAATCCACCAGATCACAAAAGCTCCTCAAAAGAGGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000902 GO:0001882 GO:0001883 GO:0003674 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005886 GO:0006810 GO:0006888 GO:0006996 GO:0007010 GO:0007275 GO:0008150 GO:0009653 GO:0009826 GO:0009832 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0017076 GO:0019001 GO:0022622 GO:0030029 GO:0030036 GO:0030154 GO:0031090 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032989 GO:0035639 GO:0036094 GO:0040007 GO:0042546 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0048364 GO:0048589 GO:0048731 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060560 GO:0071554 GO:0071669 GO:0071840 GO:0071944 GO:0090558 GO:0090627 GO:0097159 GO:0097367 GO:0098588 GO:0098805 GO:0099402 GO:1901265 GO:1901363 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RHD3

Protein Analysis

795

Amino Acids

88.82

Weight (kDa)

5.41

Isoelectric Point (pI)

43.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
URGCP_GTPase	PF25683	12 - 172	2.8e-09	URGCP-like GTPase domain
RHD3_GTPase	PF05879	48 - 283	6e-119	Root hair defective 3 GTP-binding protein (RHD3) GTPase domain
Sey1_3HB	PF20428	324 - 668	1.2e-134	Sey1 three-helix bundle domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0021861)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72960 AT1G72960
rosa_chinensis	RchiOBHm_Chr4g0434511
rosa_samantha	Rh4BG000200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1253
Acc16I	TGCGCA	1 cut(s)	909
Acc36I	ACCTGC	1 cut(s)	1253
AccB1I	GGYRCC	2 cut(s)	305, 1265
AccI	GTMKAC	1 cut(s)	1988
AciI	CCGC	7 cut(s)	159, 877, 1351, 1627, 2010, 2197, 2232
AclWI	GGATC	5 cut(s)	843, 1722, 1832, 1845, 1877
AcoI	YGGCCR	1 cut(s)	418
AcsI	RAATTY	3 cut(s)	544, 2093, 2192
AcuI	CTGAAG	4 cut(s)	624, 1191, 1559, 1651
AcvI	CACGTG	1 cut(s)	1694
AfaI	GTAC	5 cut(s)	36, 169, 1305, 1320, 1660
AfiI	CCNNNNNNNGG	4 cut(s)	292, 782, 1221, 1350
AflII	CTTAAG	1 cut(s)	559
AflIII	ACRYGT	2 cut(s)	399, 1693
AgeI	ACCGGT	2 cut(s)	1417, 1939
AjnI	CCWGG	2 cut(s)	2185, 2212
AleI	CACNNNNGTG	1 cut(s)	1940
Alw21I	GWGCWC	3 cut(s)	361, 1463, 2209
Alw26I	GTCTC	7 cut(s)	73, 139, 234, 706, 750, 1063, 1957
Alw44I	GTGCAC	1 cut(s)	2205
AlwI	GGATC	5 cut(s)	843, 1722, 1832, 1845, 1877
AlwNI	CAGNNNCTG	1 cut(s)	1505
AoxI	GGCC	2 cut(s)	115, 418
ApaLI	GTGCAC	1 cut(s)	2205
ApeKI	GCWGC	4 cut(s)	431, 1075, 2276, 2300
ApoI	RAATTY	3 cut(s)	544, 2093, 2192
AsiGI	ACCGGT	2 cut(s)	1417, 1939
Asp700I	GAANNNNTTC	2 cut(s)	570, 1089
AspLEI	GCGC	3 cut(s)	910, 936, 2281
AspS9I	GGNCC	3 cut(s)	146, 962, 1701
AsuC2I	CCSGG	2 cut(s)	529, 741
AsuHPI	GGTGA	3 cut(s)	478, 1205, 1576
AsuII	TTCGAA	2 cut(s)	345, 1064
AvaII	GGWCC	3 cut(s)	146, 962, 1701
AxyI	CCTNAGG	1 cut(s)	781
BaeGI	GKGCMC	2 cut(s)	308, 2209
BamHI	GGATCC	1 cut(s)	1837
BanI	GGYRCC	2 cut(s)	305, 1265
BanII	GRGCYC	1 cut(s)	2220
BarI	GAAGNNNNNNTAC	2 cut(s)	1458, 1490
BbrPI	CACGTG	1 cut(s)	1694
BbsI	GAAGAC	2 cut(s)	1820, 2295
Bbv12I	GWGCWC	3 cut(s)	361, 1463, 2209
BbvI	GCAGC	4 cut(s)	418, 1087, 2288, 2312
BccI	CCATC	7 cut(s)	47, 202, 305, 1134, 1277, 1430, 1924
BceAI	ACGGC	2 cut(s)	354, 405
BcgI	CGANNNNNNTGC	2 cut(s)	872, 906
BciT130I	CCWGG	2 cut(s)	2187, 2214
BclI	TGATCA	2 cut(s)	393, 1932
BcnI	CCSGG	2 cut(s)	529, 741
BcoDI	GTCTC	7 cut(s)	73, 139, 234, 706, 750, 1063, 1957
BfaI	CTAG	2 cut(s)	705, 1316
BfmI	CTRYAG	3 cut(s)	1497, 1824, 2298
BfrI	CTTAAG	1 cut(s)	559
BfuAI	ACCTGC	1 cut(s)	1253
BglII	AGATCT	2 cut(s)	807, 1058
BisI	GCNGC	4 cut(s)	432, 1076, 2277, 2301
BlsI	GCNGC	4 cut(s)	433, 1077, 2278, 2302
Bme1390I	CCNGG	4 cut(s)	529, 741, 2187, 2214
Bme18I	GGWCC	3 cut(s)	146, 962, 1701
BmgT120I	GGNCC	3 cut(s)	146, 962, 1701
BmiI	GGNNCC	6 cut(s)	147, 307, 1267, 1441, 1839, 1914
BmrFI	CCNGG	4 cut(s)	529, 741, 2187, 2214
BmsI	GCATC	5 cut(s)	84, 202, 462, 1626, 1878
BpiI	GAAGAC	2 cut(s)	1820, 2295
BpmI	CTGGAG	2 cut(s)	774, 832
Bpu14I	TTCGAA	2 cut(s)	345, 1064
BpuEI	CTTGAG	1 cut(s)	1983
BpuMI	CCSGG	2 cut(s)	529, 741
Bsa29I	ATCGAT	2 cut(s)	78, 2225
BsaAI	YACGTR	3 cut(s)	491, 1322, 1694
BsaI	GGTCTC	1 cut(s)	234
BsaJI	CCNNGG	4 cut(s)	2054, 2080, 2185, 2213
BsaWI	WCCGGW	3 cut(s)	1417, 1703, 1939
BsaXI	ACNNNNNCTCC	2 cut(s)	2354, 2384
Bsc4I	CCNNNNNNNGG	4 cut(s)	292, 782, 1221, 1350
Bse118I	RCCGGY	4 cut(s)	266, 959, 1417, 1939
Bse1I	ACTGG	7 cut(s)	663, 935, 945, 965, 1377, 1893, 2045
Bse21I	CCTNAGG	1 cut(s)	781
Bse3DI	GCAATG	2 cut(s)	1952, 2206
BseBI	CCWGG	2 cut(s)	2187, 2214
BseCI	ATCGAT	2 cut(s)	78, 2225
BseDI	CCNNGG	4 cut(s)	2054, 2080, 2185, 2213
BseGI	GGATG	8 cut(s)	10, 29, 217, 337, 949, 1441, 1798, 1912
BseLI	CCNNNNNNNGG	4 cut(s)	292, 782, 1221, 1350
BseMI	GCAATG	2 cut(s)	1952, 2206
BseMII	CTCAG	4 cut(s)	245, 795, 1525, 1779
BseNI	ACTGG	7 cut(s)	663, 935, 945, 965, 1377, 1893, 2045
BseRI	GAGGAG	7 cut(s)	339, 612, 686, 780, 962, 2040, 2362
BseSI	GKGCMC	2 cut(s)	308, 2209
BseXI	GCAGC	4 cut(s)	418, 1087, 2288, 2312
BsgI	GTGCAG	2 cut(s)	819, 1281
BshFI	GGCC	2 cut(s)	117, 420
BshNI	GGYRCC	2 cut(s)	305, 1265
BshTI	ACCGGT	2 cut(s)	1417, 1939
BshVI	ATCGAT	2 cut(s)	78, 2225
BsiHKAI	GWGCWC	3 cut(s)	361, 1463, 2209
BsiSI	CCGG	7 cut(s)	267, 529, 740, 960, 1418, 1704, 1940
BslFI	GGGAC	3 cut(s)	1143, 1304, 1322
BslI	CCNNNNNNNGG	4 cut(s)	292, 782, 1221, 1350
BsmAI	GTCTC	7 cut(s)	73, 139, 234, 706, 750, 1063, 1957
BsmFI	GGGAC	3 cut(s)	1143, 1304, 1322
BsmI	GAATGC	3 cut(s)	215, 341, 727
BsnI	GGCC	2 cut(s)	117, 420
Bso31I	GGTCTC	1 cut(s)	234
Bsp119I	TTCGAA	2 cut(s)	345, 1064
Bsp1286I	GDGCHC	5 cut(s)	308, 361, 1463, 2209, 2220
Bsp1407I	TGTACA	1 cut(s)	34
Bsp19I	CCATGG	1 cut(s)	2054
BspACI	CCGC	7 cut(s)	159, 877, 1351, 1627, 2010, 2197, 2232
BspANI	GGCC	2 cut(s)	117, 420
BspCNI	CTCAG	4 cut(s)	244, 794, 1524, 1778
BspDI	ATCGAT	2 cut(s)	78, 2225
BspHI	TCATGA	2 cut(s)	1399, 2097
BspLI	GGNNCC	6 cut(s)	147, 307, 1267, 1441, 1839, 1914
BspMAI	CTGCAG	2 cut(s)	1501, 2302
BspMI	ACCTGC	1 cut(s)	1253
BspPI	GGATC	5 cut(s)	843, 1722, 1832, 1845, 1877
BspQI	GCTCTTC	1 cut(s)	1562
BspT104I	TTCGAA	2 cut(s)	345, 1064
BspT107I	GGYRCC	2 cut(s)	305, 1265
BspTI	CTTAAG	1 cut(s)	559
BspTNI	GGTCTC	1 cut(s)	234
BsrDI	GCAATG	2 cut(s)	1952, 2206
BsrFI	RCCGGY	4 cut(s)	266, 959, 1417, 1939
BsrGI	TGTACA	1 cut(s)	34
BsrI	ACTGG	7 cut(s)	663, 935, 945, 965, 1377, 1893, 2045
BssAI	RCCGGY	4 cut(s)	266, 959, 1417, 1939
BssECI	CCNNGG	4 cut(s)	2054, 2080, 2185, 2213
BssNAI	GTATAC	1 cut(s)	1989
BssT1I	CCWWGG	2 cut(s)	2054, 2080
Bst1107I	GTATAC	1 cut(s)	1989
Bst2UI	CCWGG	2 cut(s)	2187, 2214
Bst6I	CTCTTC	5 cut(s)	757, 1061, 1248, 1562, 1611
BstAFI	CTTAAG	1 cut(s)	559
BstAPI	GCANNNNNTGC	3 cut(s)	263, 797, 2066
BstAUI	TGTACA	1 cut(s)	34
BstBAI	YACGTR	3 cut(s)	491, 1322, 1694
BstBI	TTCGAA	2 cut(s)	345, 1064
BstC8I	GCNNGC	3 cut(s)	255, 957, 1234
BstDEI	CTNAG	6 cut(s)	231, 626, 781, 1511, 1765, 1860
BstDSI	CCRYGG	1 cut(s)	2054
BstEII	GGTNACC	1 cut(s)	2043
BstF5I	GGATG	8 cut(s)	10, 29, 217, 337, 949, 1441, 1798, 1912
BstHHI	GCGC	3 cut(s)	910, 936, 2281
BstMAI	GTCTC	7 cut(s)	73, 139, 234, 706, 750, 1063, 1957
BstNI	CCWGG	2 cut(s)	2187, 2214
BstNSI	RCATGY	1 cut(s)	403
BstPI	GGTNACC	1 cut(s)	2043
BstSCI	CCNGG	4 cut(s)	527, 739, 2185, 2212
BstSFI	CTRYAG	3 cut(s)	1497, 1824, 2298
BstSLI	GKGCMC	2 cut(s)	308, 2209
BstV1I	GCAGC	4 cut(s)	418, 1087, 2288, 2312
BstV2I	GAAGAC	2 cut(s)	1820, 2295
BstX2I	RGATCY	5 cut(s)	807, 835, 1058, 1714, 1837
BstXI	CCANNNNNNTGG	2 cut(s)	188, 1698
BstYI	RGATCY	5 cut(s)	807, 835, 1058, 1714, 1837
BstZ17I	GTATAC	1 cut(s)	1989
Bsu15I	ATCGAT	2 cut(s)	78, 2225
Bsu36I	CCTNAGG	1 cut(s)	781
BsuRI	GGCC	2 cut(s)	117, 420
BsuTUI	ATCGAT	2 cut(s)	78, 2225
BtgI	CCRYGG	1 cut(s)	2054
BtsCI	GGATG	8 cut(s)	10, 29, 217, 337, 949, 1441, 1798, 1912
BtsIMutI	CAGTG	3 cut(s)	1886, 1998, 2162
BveI	ACCTGC	1 cut(s)	1253
Cac8I	GCNNGC	3 cut(s)	255, 957, 1234
CaiI	CAGNNNCTG	1 cut(s)	1505
CciI	TCATGA	2 cut(s)	1399, 2097
CfoI	GCGC	3 cut(s)	910, 936, 2281
Cfr10I	RCCGGY	4 cut(s)	266, 959, 1417, 1939
Cfr13I	GGNCC	3 cut(s)	146, 962, 1701
ClaI	ATCGAT	2 cut(s)	78, 2225
CseI	GACGC	1 cut(s)	2179
Csp6I	GTAC	5 cut(s)	35, 168, 1304, 1319, 1659
CspAI	ACCGGT	2 cut(s)	1417, 1939
CviQI	GTAC	5 cut(s)	35, 168, 1304, 1319, 1659
DdeI	CTNAG	6 cut(s)	231, 626, 781, 1511, 1765, 1860
DraI	TTTAAA	1 cut(s)	691
EaeI	YGGCCR	1 cut(s)	418
Eam1104I	CTCTTC	5 cut(s)	757, 1061, 1248, 1562, 1611
EarI	CTCTTC	5 cut(s)	757, 1061, 1248, 1562, 1611
Eco130I	CCWWGG	2 cut(s)	2054, 2080
Eco24I	GRGCYC	1 cut(s)	2220
Eco31I	GGTCTC	1 cut(s)	234
Eco32I	GATATC	3 cut(s)	385, 2182, 2229
Eco47I	GGWCC	3 cut(s)	146, 962, 1701
Eco57I	CTGAAG	4 cut(s)	624, 1191, 1559, 1651
Eco72I	CACGTG	1 cut(s)	1694
Eco81I	CCTNAGG	1 cut(s)	781
Eco91I	GGTNACC	1 cut(s)	2043
EcoO109I	RGGNCCY	1 cut(s)	146
EcoO65I	GGTNACC	1 cut(s)	2043
EcoRI	GAATTC	2 cut(s)	2093, 2192
EcoRII	CCWGG	2 cut(s)	2185, 2212
EcoRV	GATATC	3 cut(s)	385, 2182, 2229
EcoT14I	CCWWGG	2 cut(s)	2054, 2080
EcoT22I	ATGCAT	2 cut(s)	217, 729
EcoT38I	GRGCYC	1 cut(s)	2220
ErhI	CCWWGG	2 cut(s)	2054, 2080
FalI	AAGNNNNNCTT	4 cut(s)	1074, 1106, 1701, 1733
FaqI	GGGAC	3 cut(s)	1143, 1304, 1322
FauI	CCCGC	2 cut(s)	152, 1620
FbaI	TGATCA	2 cut(s)	393, 1932
FblI	GTMKAC	1 cut(s)	1988
Fnu4HI	GCNGC	4 cut(s)	432, 1076, 2277, 2301
FokI	GGATG	8 cut(s)	17, 36, 224, 344, 956, 1448, 1805, 1919
FriOI	GRGCYC	1 cut(s)	2220
Fsp4HI	GCNGC	4 cut(s)	432, 1076, 2277, 2301
FspBI	CTAG	2 cut(s)	705, 1316
FspI	TGCGCA	1 cut(s)	909
GlaI	GCGC	3 cut(s)	909, 935, 2280
GluI	GCNGC	4 cut(s)	432, 1076, 2277, 2301
GsuI	CTGGAG	2 cut(s)	774, 832
HaeIII	GGCC	2 cut(s)	117, 420
HapII	CCGG	7 cut(s)	267, 529, 740, 960, 1418, 1704, 1940
HgaI	GACGC	1 cut(s)	2179
HhaI	GCGC	3 cut(s)	910, 936, 2281
Hin6I	GCGC	3 cut(s)	908, 934, 2279
HinP1I	GCGC	3 cut(s)	908, 934, 2279
HindIII	AAGCTT	4 cut(s)	1088, 1404, 1425, 1472
HpaII	CCGG	7 cut(s)	267, 529, 740, 960, 1418, 1704, 1940
HphI	GGTGA	3 cut(s)	478, 1205, 1576
Hpy166II	GTNNAC	6 cut(s)	170, 425, 488, 1989, 2169, 2207
Hpy8I	GTNNAC	6 cut(s)	170, 425, 488, 1989, 2169, 2207
Hpy99I	CGWCG	2 cut(s)	50, 2322
HpyAV	CCTTC	9 cut(s)	219, 670, 854, 1043, 1190, 1583, 1600, 1639, 2159
HpyCH4IV	ACGT	4 cut(s)	490, 1321, 1454, 1693
HpyF3I	CTNAG	6 cut(s)	231, 626, 781, 1511, 1765, 1860
HpySE526I	ACGT	4 cut(s)	490, 1321, 1454, 1693
HspAI	GCGC	3 cut(s)	908, 934, 2279
Ksp22I	TGATCA	2 cut(s)	393, 1932
LguI	GCTCTTC	1 cut(s)	1562
LmnI	GCTCC	3 cut(s)	1439, 1458, 2375
Lsp1109I	GCAGC	4 cut(s)	418, 1087, 2288, 2312
LweI	GCATC	5 cut(s)	84, 202, 462, 1626, 1878
MaeI	CTAG	2 cut(s)	705, 1316
MaeII	ACGT	4 cut(s)	490, 1321, 1454, 1693
MaeIII	GTNAC	5 cut(s)	407, 629, 1322, 1993, 2043
MfeI	CAATTG	2 cut(s)	1285, 2174
MflI	RGATCY	5 cut(s)	807, 835, 1058, 1714, 1837
MhlI	GDGCHC	5 cut(s)	308, 361, 1463, 2209, 2220
MlyI	GAGTC	5 cut(s)	825, 1397, 1503, 1990, 2095
MmeI	TCCRAC	4 cut(s)	519, 1120, 1413, 1865
Mph1103I	ATGCAT	2 cut(s)	217, 729
MroXI	GAANNNNTTC	2 cut(s)	570, 1089
MseI	TTAA	5 cut(s)	90, 560, 690, 1161, 2108
MslI	CAYNNNNRTG	3 cut(s)	1482, 1940, 2252
MspCI	CTTAAG	1 cut(s)	559
MspI	CCGG	7 cut(s)	267, 529, 740, 960, 1418, 1704, 1940
MspR9I	CCNGG	4 cut(s)	529, 741, 2187, 2214
MunI	CAATTG	2 cut(s)	1285, 2174
Mva1269I	GAATGC	3 cut(s)	215, 341, 727
MvaI	CCWGG	2 cut(s)	2187, 2214
NciI	CCSGG	2 cut(s)	529, 741
NcoI	CCATGG	1 cut(s)	2054
NlaIV	GGNNCC	6 cut(s)	147, 307, 1267, 1441, 1839, 1914
NmuCI	GTSAC	4 cut(s)	407, 629, 1322, 1993
NsbI	TGCGCA	1 cut(s)	909
NsiI	ATGCAT	2 cut(s)	217, 729
NspI	RCATGY	1 cut(s)	403
NspV	TTCGAA	2 cut(s)	345, 1064
OliI	CACNNNNGTG	1 cut(s)	1940
PagI	TCATGA	2 cut(s)	1399, 2097
PaqCI	CACCTGC	1 cut(s)	1253
PciI	ACATGT	1 cut(s)	399
PciSI	GCTCTTC	1 cut(s)	1562
PctI	GAATGC	3 cut(s)	215, 341, 727
PdmI	GAANNNNTTC	2 cut(s)	570, 1089
PfeI	GAWTC	7 cut(s)	587, 772, 1105, 1493, 1676, 1682, 2085
PinAI	ACCGGT	2 cut(s)	1417, 1939
PkrI	GCNGC	4 cut(s)	433, 1077, 2278, 2302
PleI	GAGTC	5 cut(s)	824, 1396, 1503, 1990, 2095
PmaCI	CACGTG	1 cut(s)	1694
PmlI	CACGTG	1 cut(s)	1694
PpsI	GAGTC	5 cut(s)	824, 1396, 1503, 1990, 2095
Ppu21I	YACGTR	3 cut(s)	491, 1322, 1694
PpuMI	RGGWCCY	1 cut(s)	146
PscI	ACATGT	1 cut(s)	399
Psp5II	RGGWCCY	1 cut(s)	146
Psp6I	CCWGG	2 cut(s)	2185, 2212
PspCI	CACGTG	1 cut(s)	1694
PspEI	GGTNACC	1 cut(s)	2043
PspGI	CCWGG	2 cut(s)	2185, 2212
PspN4I	GGNNCC	6 cut(s)	147, 307, 1267, 1441, 1839, 1914
PspPI	GGNCC	3 cut(s)	146, 962, 1701
PspPPI	RGGWCCY	1 cut(s)	146
PstI	CTGCAG	2 cut(s)	1501, 2302
PstNI	CAGNNNCTG	1 cut(s)	1505
PsuI	RGATCY	5 cut(s)	807, 835, 1058, 1714, 1837
RsaI	GTAC	5 cut(s)	36, 169, 1305, 1320, 1660
RsaNI	GTAC	5 cut(s)	35, 168, 1304, 1319, 1659
RseI	CAYNNNNRTG	3 cut(s)	1482, 1940, 2252
SapI	GCTCTTC	1 cut(s)	1562
SaqAI	TTAA	5 cut(s)	90, 560, 690, 1161, 2108
SatI	GCNGC	4 cut(s)	432, 1076, 2277, 2301
Sau96I	GGNCC	3 cut(s)	146, 962, 1701
SchI	GAGTC	5 cut(s)	825, 1397, 1503, 1990, 2095
ScrFI	CCNGG	4 cut(s)	529, 741, 2187, 2214
SduI	GDGCHC	5 cut(s)	308, 361, 1463, 2209, 2220
SfaNI	GCATC	5 cut(s)	84, 202, 462, 1626, 1878
SfcI	CTRYAG	3 cut(s)	1497, 1824, 2298
SfuI	TTCGAA	2 cut(s)	345, 1064
SgrAI	CRCCGGYG	1 cut(s)	1939
SinI	GGWCC	3 cut(s)	146, 962, 1701
SmiMI	CAYNNNNRTG	3 cut(s)	1482, 1940, 2252
SmlI	CTYRAG	2 cut(s)	559, 1998
SmoI	CTYRAG	2 cut(s)	559, 1998
SsiI	CCGC	7 cut(s)	159, 877, 1351, 1627, 2010, 2197, 2232
SspMI	CTAG	2 cut(s)	705, 1316
StyD4I	CCNGG	4 cut(s)	527, 739, 2185, 2212
StyI	CCWWGG	2 cut(s)	2054, 2080
TaiI	ACGT	4 cut(s)	493, 1324, 1457, 1696
TatI	WGTACW	2 cut(s)	34, 167
TfiI	GAWTC	7 cut(s)	587, 772, 1105, 1493, 1676, 1682, 2085
Tru1I	TTAA	5 cut(s)	90, 560, 690, 1161, 2108
Tru9I	TTAA	5 cut(s)	90, 560, 690, 1161, 2108
TscAI	CASTG	3 cut(s)	1893, 1998, 2169
TseFI	GTSAC	4 cut(s)	407, 629, 1322, 1993
TseI	GCWGC	4 cut(s)	431, 1075, 2276, 2300
Tsp45I	GTSAC	4 cut(s)	407, 629, 1322, 1993
TspGWI	ACGGA	1 cut(s)	63
TspRI	CASTG	3 cut(s)	1893, 1998, 2169
Vha464I	CTTAAG	1 cut(s)	559
VneI	GTGCAC	1 cut(s)	2205
VpaK11BI	GGWCC	3 cut(s)	146, 962, 1701
XapI	RAATTY	3 cut(s)	544, 2093, 2192
XceI	RCATGY	1 cut(s)	403
XmiI	GTMKAC	1 cut(s)	1988
XmnI	GAANNNNTTC	2 cut(s)	570, 1089
XspI	CTAG	2 cut(s)	705, 1316
Zsp2I	ATGCAT	2 cut(s)	217, 729

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.