AT3G05650

Leucine-rich repeat-containing N-terminal domain, type 2 (InterPro IPR013210), Leucine-rich repeat (InterPro IPR001611)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011881
Physical Location & Seq
Reverse (-)
1644882 .. 1648742
3861 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05650.1

Sequence Viewer

Length: 2607 bp
ATGAAAGACTCTTGGAACTCAACGAGTATCATTCCTTTCACTTTTTCTTCTCTCATATTCTTCTTATTCACTTTTGATTTTCAAGATGTCTTTGGGGTTCCTACTAAACACTTATGTCGTCTCGAACAAAGAGATGCACTACTGGAGCTCAAGAAGGAGTTTAAGATTAAGAAGCCTTGTTTCGACGGCCTTCATCCGACGACGGAGTCATGGGCGAATAACAGTGACTGTTGCTATTGGGACGGTATCACGTGCAATGACAAGTCCGGGGAAGTGCTTGAGCTAGACCTTAGTCGTAGCTGCCTCCAAAGCCGGTTTCATTCCAACAGCAGTCTTTTTACGGTTCTAAATCTTCGTTTTCTAACCACTCTCGACCTTTCCTATAATTATTTCAGTGGCCAAATCCCGTCTTGTATTGAAAACTTTTCTCATCTTACCACTCTCGACCTTTCTAAAAACTATTTCAGTGGTGGGATTCCTTCTTCAATAGGAAATCTTTCACAACTCACTTTTCTCGACCTTTCTGGTAACGAGTTCGTTGGTGAAATGCCATTTTTTGGCAACATGAACCAGTTGACCAACTTATATGTTGATTCCAATGACCTTACTGGTATCTTCCCCCTTTCCCTACTCAATCTGAAACATCTTTCGGATTTATCACTCTCCCGCAATCAGTTCACAGGTACCCTTCCTTCTAACATGAGCTCACTCTCTAACTTGGAGTACTTTGAGGCATGGGGCAACGCTTTTACTGGAACTCTCCCTTCTTCTCTCTTCACTATTGCTTCTTTGACGTCTATTAATTTGAGAAATAACCAACTCAACGGCACTCTTGAGTTTGGGAACATATCTTCACCATCTACACTAACTGTGTTAGACATTAGCAATAACAACTTCATAGGGCCAATCCCGAAATCCATTTCCAAATTTATAAACCTTCAGGACCTTGATCTTTCTCATCTCAACACCCAAGGCCCAGTTGACTTTAGTATCTTCACGAATCTCAAGTCGCTCCAACTTCTTAACCTATCTCATTTGAACACCACCACTACGATTGACTTGAATGCACTCTTTTCCTCGCATCTCAACTCAATCTATTCAATGGATCTCTCAGGCAACCATGTTTCAGCCACAACCAAAATTTCAGTTGCAGATCATCATCCTACGCAATTGATAAGTCAGTTGTACTTGTCTGGATGCGGCATCACTGAGTTCCCGGAGCTCTTAAGAAGCCAACACAAAATGACGAATCTAGACATTTCCAACAACAAAATCAAAGGTCAGGTGCCTGGCTGGCTATGGACACTACCAAAGTTGATATTCGTTGATCTTTCCAACAACATTTTTACCGGTTTTGAAAGATCAACGGAACATGGACTATCCTTAATCACCAAACCATCTATGCAGTACTTGGTTGGCTCCAACAACAATTTCACGGGAAAGATTCCCTCTTTCATATGCGCTTTGCGCTCTCTAATCACTCTTGATTTATCTGACAACAACCTCAATGGTTCCATCCCTCCATGTATGGGAAACCTTAAGAGTACTCTTTCATTTCTAAACCTTCGTCAGAATCGTCTTGGTGGAGGTCTTCCAAGGAGTATATTTAAAAGTCTAAGGTCACTTGATGTCGGTCATAACCAACTAGTCGGAAAACTTCCTAGATCTTTCATCCGTCTCTCTGCTCTTGAAGTTCTGAATGTGGAAAACAACAGAATCAACGACACATTCCCGTTCTGGTTGTCTTCTCTCAAAAAGCTGCAAGTTCTTGTCTTACGCTCCAATGCATTCCACGGACCGATACATCATGCCTCGTTCCATACATTGCGAATCATCAACTTATCTCATAATCAATTCAGTGGAACTTTGCCAGCAAACTATTTTGTGAACTGGAATGCTATGTCATCACTTATGGCAACCGAGGATCGGTCTCAAGAGAAATACATGGGAGACTCTTTTCGATATTACCATGATTCAGTGGTTTTGATGAATAAAGGCTTAGAGATGGAGCTGGTACGCATCCTAAAAATCTACACAGCTCTCGACTTTTCTGAAAACAAATTAGAAGGAGAGATTCCAAGATCCATCGGTCTTTTGAAAGAGCTTCATGTTCTTAACTTGTCAAGCAATGCTTTCACCGGCCACATCCCATCATCTATGGGGAATCTTAGAGAGCTCGAGTCACTGGACGTTTCTCAAAACAAGCTTTCAGGAGAAATTCCACAAGAATTAGGGAACCTATCGTACCTTGCCTACATGAACTTCTCTCACAACCAGCTTGGAGGTCTAGTACCAGGGGGAACTCAGTTTCGTCGACAAAACTGCTCTTCTTTCAAGGACAACCCTGGACTTTATGGCTCTTCACTTGAAGAAGTTTGTTTAGATATTCATGCACCAGCACCGCAACAACATGAACCGCCAGAGTTAGAGGAAGAAGACAGAGAGGTGTTCAGTTGGATAGCAGCTGCAATAGGATTCGGACCTGGTATTGCCTTTGGATTGACGATTAGATACATACTGGTTTTCTACAAACCAGATTGGTTCATGCATACCTTTGGCCACTTACAACCCTCTGCACATGAAAAAAGATTGAGGAGAAAGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

868

Amino Acids

97.3

Weight (kDa)

6.28

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 42 - 86 4.7e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 109 - 350 2.2e-17 Leucine-rich repeat region
LRR_8 PF13855 121 - 178 5.6e-09 Leucine rich repeat
LRR_8 PF13855 191 - 248 7e-06 Leucine rich repeat
LRR_8 PF13855 517 - 573 7.2e-06 Leucine rich repeat
LRR_8 PF13855 538 - 596 1.7e-08 Leucine rich repeat
LRR_8 PF13855 562 - 619 1.2e-06 Leucine rich repeat
LRR_14 PF23598 690 - 753 7.7e-07 Leucine-rich repeat region
LRR_8 PF13855 701 - 760 5.9e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 932
AasI GACNNNNNNGTC 1 cut(s) 205
AatII GACGTC 1 cut(s) 797
Acc65I GGTACC 1 cut(s) 683
AccB1I GGYRCC 2 cut(s) 683, 1285
AccB7I CCANNNNNTGG 1 cut(s) 557
AccI GTMKAC 1 cut(s) 2314
AciI CCGC 4 cut(s) 667, 1200, 2402, 2417
AclWI GGATC 3 cut(s) 1113, 1932, 2076
AcoI YGGCCR 3 cut(s) 397, 2140, 2557
AcsI RAATTY 3 cut(s) 926, 1140, 2217
AcuI CTGAAG 1 cut(s) 923
AcvI CACGTG 1 cut(s) 252
AcyI GRCGYC 1 cut(s) 794
AfaI GTAC 8 cut(s) 685, 725, 1187, 1409, 1546, 2016, 2246, 2292
AfiI CCNNNNNNNGG 3 cut(s) 557, 1529, 1926
AflII CTTAAG 2 cut(s) 1225, 1538
AgeI ACCGGT 1 cut(s) 1349
AhlI ACTAGT 1 cut(s) 1645
AjnI CCWGG 4 cut(s) 1288, 2293, 2344, 2482
AjuI GAANNNNNNNTTGG 4 cut(s) 300, 332, 1415, 1447
Alw21I GWGCWC 4 cut(s) 150, 707, 1224, 2178
Alw26I GTCTC 4 cut(s) 125, 1682, 1935, 1944
AlwI GGATC 3 cut(s) 1113, 1932, 2076
AlwNI CAGNNNCTG 1 cut(s) 228
Ama87I CYCGRG 1 cut(s) 2177
AoxI GGCC 6 cut(s) 187, 397, 902, 973, 2140, 2557
ApeKI GCWGC 4 cut(s) 300, 1759, 2462, 2465
ApoI RAATTY 3 cut(s) 926, 1140, 2217
ArsI GACNNNNNNTTYG 2 cut(s) 992, 1024
AseI ATTAAT 1 cut(s) 801
AsiGI ACCGGT 1 cut(s) 1349
Asp700I GAANNNNTTC 1 cut(s) 496
Asp718I GGTACC 1 cut(s) 683
AspLEI GCGC 2 cut(s) 1463, 1470
AspS9I GGNCC 5 cut(s) 902, 943, 974, 1796, 2480
AsuC2I CCSGG 2 cut(s) 268, 1217
AsuHPI GGTGA 4 cut(s) 554, 846, 1381, 2128
AvaI CYCGRG 1 cut(s) 2177
AvaII GGWCC 3 cut(s) 943, 1796, 2480
BaeI ACNNNNGTAYC 2 cut(s) 973, 1006
BalI TGGCCA 2 cut(s) 399, 2559
BanI GGYRCC 2 cut(s) 683, 1285
BanII GRGCYC 4 cut(s) 150, 707, 1224, 2178
BarI GAAGNNNNNNTAC 2 cut(s) 676, 708
BbrPI CACGTG 1 cut(s) 252
BbsI GAAGAC 3 cut(s) 1583, 1737, 2442
Bbv12I GWGCWC 4 cut(s) 150, 707, 1224, 2178
BbvI GCAGC 4 cut(s) 287, 1746, 2452, 2474
BccI CCATC 6 cut(s) 865, 1405, 1523, 1999, 2093, 2158
BceAI ACGGC 2 cut(s) 202, 841
BcgI CGANNNNNNTGC 2 cut(s) 2304, 2338
BciT130I CCWGG 4 cut(s) 1290, 2295, 2346, 2484
BcnI CCSGG 2 cut(s) 268, 1217
BcoDI GTCTC 4 cut(s) 125, 1682, 1935, 1944
BcuI ACTAGT 1 cut(s) 1645
BfaI CTAG 5 cut(s) 284, 1253, 1646, 1662, 2288
BfrI CTTAAG 2 cut(s) 1225, 1538
BglI GCCNNNNNGGC 1 cut(s) 1294
BglII AGATCT 1 cut(s) 1664
BisI GCNGC 5 cut(s) 301, 1201, 1760, 2463, 2466
BlsI GCNGC 5 cut(s) 302, 1202, 1761, 2464, 2467
BmcAI AGTACT 3 cut(s) 725, 1409, 1546
Bme1390I CCNGG 6 cut(s) 268, 1217, 1290, 2295, 2346, 2484
Bme18I GGWCC 3 cut(s) 943, 1796, 2480
BmeT110I CYCGRG 1 cut(s) 2177
BmgT120I GGNCC 5 cut(s) 902, 943, 974, 1796, 2480
BmiI GGNNCC 6 cut(s) 99, 685, 1287, 1420, 1513, 2237
BmrFI CCNGG 6 cut(s) 268, 1217, 1290, 2295, 2346, 2484
BmrI ACTGGG 1 cut(s) 971
BmsI GCATC 5 cut(s) 124, 1090, 1187, 1212, 2028
BmuI ACTGGG 1 cut(s) 971
BoxI GACNNNNGTC 1 cut(s) 291
BpiI GAAGAC 3 cut(s) 1583, 1737, 2442
BpmI CTGGAG 1 cut(s) 164
BpuEI CTTGAG 5 cut(s) 134, 299, 854, 989, 1917
BpuMI CCSGG 2 cut(s) 268, 1217
BsaAI YACGTR 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 1158
BsaHI GRCGYC 1 cut(s) 794
BsaI GGTCTC 1 cut(s) 1935
BsaJI CCNNGG 7 cut(s) 267, 970, 1595, 1792, 1920, 2294, 2344
BsaWI WCCGGW 1 cut(s) 1349
BsaXI ACNNNNNCTCC 2 cut(s) 2274, 2304
Bsc4I CCNNNNNNNGG 3 cut(s) 557, 1529, 1926
Bse118I RCCGGY 3 cut(s) 312, 1349, 2138
Bse1I ACTGG 8 cut(s) 147, 571, 613, 757, 977, 1895, 2190, 2523
Bse3DI GCAATG 3 cut(s) 262, 1823, 2134
Bse8I GATNNNNATC 1 cut(s) 1158
BseBI CCWGG 4 cut(s) 1290, 2295, 2346, 2484
BseDI CCNNGG 7 cut(s) 267, 970, 1595, 1792, 1920, 2294, 2344
BseGI GGATG 7 cut(s) 193, 1159, 1202, 1515, 1671, 2019, 2145
BseJI GATNNNNATC 1 cut(s) 1158
BseLI CCNNNNNNNGG 3 cut(s) 557, 1529, 1926
BseMI GCAATG 3 cut(s) 262, 1823, 2134
BseMII CTCAG 3 cut(s) 1125, 1200, 2318
BseNI ACTGG 8 cut(s) 147, 571, 613, 757, 977, 1895, 2190, 2523
BseXI GCAGC 4 cut(s) 287, 1746, 2452, 2474
BsgI GTGCAG 1 cut(s) 2559
BshFI GGCC 6 cut(s) 189, 399, 904, 975, 2142, 2559
BshNI GGYRCC 2 cut(s) 683, 1285
BshTI ACCGGT 1 cut(s) 1349
BsiHKAI GWGCWC 4 cut(s) 150, 707, 1224, 2178
BsiHKCI CYCGRG 1 cut(s) 2177
BsiSI CCGG 5 cut(s) 267, 313, 1217, 1350, 2139
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 3 cut(s) 557, 1529, 1926
BsmAI GTCTC 4 cut(s) 125, 1682, 1935, 1944
BsmBI CGTCTC 2 cut(s) 125, 1682
BsmFI GGGAC 1 cut(s) 254
BsmI GAATGC 3 cut(s) 1069, 1787, 1900
BsnI GGCC 6 cut(s) 189, 399, 904, 975, 2142, 2559
Bso31I GGTCTC 1 cut(s) 1935
BsoBI CYCGRG 1 cut(s) 2177
Bsp1286I GDGCHC 4 cut(s) 150, 707, 1224, 2178
Bsp143I GATC 8 cut(s) 949, 1105, 1153, 1327, 1361, 1664, 1924, 2081
BspACI CCGC 4 cut(s) 667, 1200, 2402, 2417
BspANI GGCC 6 cut(s) 189, 399, 904, 975, 2142, 2559
BspCNI CTCAG 3 cut(s) 1124, 1201, 2317
BspLI GGNNCC 6 cut(s) 99, 685, 1287, 1420, 1513, 2237
BspPI GGATC 3 cut(s) 1113, 1932, 2076
BspQI GCTCTTC 2 cut(s) 2332, 2365
BspT107I GGYRCC 2 cut(s) 683, 1285
BspTI CTTAAG 2 cut(s) 1225, 1538
BspTNI GGTCTC 1 cut(s) 1935
BsrDI GCAATG 3 cut(s) 262, 1823, 2134
BsrFI RCCGGY 3 cut(s) 312, 1349, 2138
BsrI ACTGG 8 cut(s) 147, 571, 613, 757, 977, 1895, 2190, 2523
BssAI RCCGGY 3 cut(s) 312, 1349, 2138
BssECI CCNNGG 7 cut(s) 267, 970, 1595, 1792, 1920, 2294, 2344
BssMI GATC 8 cut(s) 949, 1105, 1153, 1327, 1361, 1664, 1924, 2081
BssNI GRCGYC 1 cut(s) 794
BssT1I CCWWGG 2 cut(s) 970, 1595
Bst2UI CCWGG 4 cut(s) 1290, 2295, 2346, 2484
Bst4CI ACNGT 5 cut(s) 224, 230, 245, 343, 871
Bst6I CTCTTC 3 cut(s) 779, 2332, 2365
BstACI GRCGYC 1 cut(s) 794
BstAFI CTTAAG 2 cut(s) 1225, 1538
BstBAI YACGTR 1 cut(s) 252
BstC8I GCNNGC 2 cut(s) 1295, 1872
BstDEI CTNAG 7 cut(s) 290, 1111, 1209, 1616, 1999, 2168, 2304
BstDSI CCRYGG 1 cut(s) 1792
BstF5I GGATG 7 cut(s) 193, 1159, 1202, 1515, 1671, 2019, 2145
BstHHI GCGC 2 cut(s) 1463, 1470
BstKTI GATC 8 cut(s) 952, 1108, 1156, 1330, 1364, 1667, 1927, 2084
BstMAI GTCTC 4 cut(s) 125, 1682, 1935, 1944
BstMBI GATC 8 cut(s) 949, 1105, 1153, 1327, 1361, 1664, 1924, 2081
BstMWI GCNNNNNNNGC 3 cut(s) 309, 1294, 1467
BstNI CCWGG 4 cut(s) 1290, 2295, 2346, 2484
BstPAI GACNNNNGTC 1 cut(s) 291
BstSCI CCNGG 6 cut(s) 266, 1215, 1288, 2293, 2344, 2482
BstV1I GCAGC 4 cut(s) 287, 1746, 2452, 2474
BstV2I GAAGAC 3 cut(s) 1583, 1737, 2442
BstX2I RGATCY 3 cut(s) 1105, 1664, 2081
BstYI RGATCY 3 cut(s) 1105, 1664, 2081
BsuRI GGCC 6 cut(s) 189, 399, 904, 975, 2142, 2559
BtgI CCRYGG 1 cut(s) 1792
BtsCI GGATG 7 cut(s) 193, 1159, 1202, 1515, 1671, 2019, 2145
BtsIMutI CAGTG 7 cut(s) 229, 400, 472, 1206, 1864, 1983, 2183
Cac8I GCNNGC 2 cut(s) 1295, 1872
CaiI CAGNNNCTG 1 cut(s) 228
CfoI GCGC 2 cut(s) 1463, 1470
Cfr10I RCCGGY 3 cut(s) 312, 1349, 2138
Cfr13I GGNCC 5 cut(s) 902, 943, 974, 1796, 2480
CpoI CGGWCCG 1 cut(s) 1796
CsiI ACCWGGT 1 cut(s) 2482
Csp6I GTAC 8 cut(s) 684, 724, 1186, 1408, 1545, 2015, 2245, 2291
CspAI ACCGGT 1 cut(s) 1349
CspCI CAANNNNNGTGG 2 cut(s) 1036, 1071
CspI CGGWCCG 1 cut(s) 1796
CviQI GTAC 8 cut(s) 684, 724, 1186, 1408, 1545, 2015, 2245, 2291
DdeI CTNAG 7 cut(s) 290, 1111, 1209, 1616, 1999, 2168, 2304
DpnI GATC 8 cut(s) 951, 1107, 1155, 1329, 1363, 1666, 1926, 2083
DpnII GATC 8 cut(s) 949, 1105, 1153, 1327, 1361, 1664, 1924, 2081
DraI TTTAAA 1 cut(s) 1609
DrdI GACNNNNNNGTC 1 cut(s) 205
DseDI GACNNNNNNGTC 1 cut(s) 205
EaeI YGGCCR 3 cut(s) 397, 2140, 2557
Eam1104I CTCTTC 3 cut(s) 779, 2332, 2365
EarI CTCTTC 3 cut(s) 779, 2332, 2365
Ecl136II GAGCTC 4 cut(s) 148, 705, 1222, 2176
Eco130I CCWWGG 2 cut(s) 970, 1595
Eco24I GRGCYC 4 cut(s) 150, 707, 1224, 2178
Eco31I GGTCTC 1 cut(s) 1935
Eco47I GGWCC 3 cut(s) 943, 1796, 2480
Eco53kI GAGCTC 4 cut(s) 148, 705, 1222, 2176
Eco57I CTGAAG 1 cut(s) 923
Eco72I CACGTG 1 cut(s) 252
Eco88I CYCGRG 1 cut(s) 2177
EcoICRI GAGCTC 4 cut(s) 148, 705, 1222, 2176
EcoO109I RGGNCCY 1 cut(s) 943
EcoRII CCWGG 4 cut(s) 1288, 2293, 2344, 2482
EcoT14I CCWWGG 2 cut(s) 970, 1595
EcoT22I ATGCAT 2 cut(s) 1789, 2550
EcoT38I GRGCYC 4 cut(s) 150, 707, 1224, 2178
ErhI CCWWGG 2 cut(s) 970, 1595
Esp3I CGTCTC 2 cut(s) 125, 1682
FalI AAGNNNNNCTT 2 cut(s) 2116, 2148
FaqI GGGAC 1 cut(s) 254
FauI CCCGC 1 cut(s) 674
FauNDI CATATG 1 cut(s) 1457
FblI GTMKAC 1 cut(s) 2314
Fnu4HI GCNGC 5 cut(s) 301, 1201, 1760, 2463, 2466
FokI GGATG 7 cut(s) 180, 1146, 1209, 1502, 1658, 2006, 2132
FriOI GRGCYC 4 cut(s) 150, 707, 1224, 2178
Fsp4HI GCNGC 5 cut(s) 301, 1201, 1760, 2463, 2466
FspBI CTAG 5 cut(s) 284, 1253, 1646, 1662, 2288
GlaI GCGC 2 cut(s) 1462, 1469
GluI GCNGC 5 cut(s) 301, 1201, 1760, 2463, 2466
GsuI CTGGAG 1 cut(s) 164
HaeIII GGCC 6 cut(s) 189, 399, 904, 975, 2142, 2559
HapII CCGG 5 cut(s) 267, 313, 1217, 1350, 2139
HhaI GCGC 2 cut(s) 1463, 1470
Hin1I GRCGYC 1 cut(s) 794
Hin6I GCGC 2 cut(s) 1461, 1468
HinP1I GCGC 2 cut(s) 1461, 1468
HincII GTYRAC 3 cut(s) 576, 982, 2315
HindII GTYRAC 3 cut(s) 576, 982, 2315
HindIII AAGCTT 1 cut(s) 2204
HpaII CCGG 5 cut(s) 267, 313, 1217, 1350, 2139
HphI GGTGA 4 cut(s) 554, 846, 1381, 2128
Hpy166II GTNNAC 5 cut(s) 576, 678, 982, 1888, 2315
Hpy188I TCNGA 9 cut(s) 198, 639, 652, 1495, 1572, 1652, 1698, 2053, 2480
Hpy8I GTNNAC 5 cut(s) 576, 678, 982, 1888, 2315
Hpy99I CGWCG 4 cut(s) 188, 202, 205, 2316
HpyAV CCTTC 9 cut(s) 148, 200, 489, 698, 702, 774, 947, 1574, 2060
HpyCH4III ACNGT 5 cut(s) 224, 230, 245, 343, 871
HpyCH4IV ACGT 3 cut(s) 251, 794, 2190
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 1294, 1467
HpyF3I CTNAG 7 cut(s) 290, 1111, 1209, 1616, 1999, 2168, 2304
HpySE526I ACGT 3 cut(s) 251, 794, 2190
Hsp92I GRCGYC 1 cut(s) 794
HspAI GCGC 2 cut(s) 1461, 1468
KpnI GGTACC 1 cut(s) 687
Kzo9I GATC 8 cut(s) 949, 1105, 1153, 1327, 1361, 1664, 1924, 2081
LguI GCTCTTC 2 cut(s) 2332, 2365
LmnI GCTCC 6 cut(s) 145, 1017, 1219, 1424, 1784, 2008
Lsp1109I GCAGC 4 cut(s) 287, 1746, 2452, 2474
LweI GCATC 5 cut(s) 124, 1090, 1187, 1212, 2028
MabI ACCWGGT 1 cut(s) 2482
MaeI CTAG 5 cut(s) 284, 1253, 1646, 1662, 2288
MaeII ACGT 3 cut(s) 251, 794, 2190
MaeIII GTNAC 4 cut(s) 224, 527, 1620, 2181
MalI GATC 8 cut(s) 951, 1107, 1155, 1329, 1363, 1666, 1926, 2083
MboI GATC 8 cut(s) 949, 1105, 1153, 1327, 1361, 1664, 1924, 2081
MfeI CAATTG 1 cut(s) 1169
MflI RGATCY 3 cut(s) 1105, 1664, 2081
MhlI GDGCHC 4 cut(s) 150, 707, 1224, 2178
MlsI TGGCCA 2 cut(s) 399, 2559
MluNI TGGCCA 2 cut(s) 399, 2559
MlyI GAGTC 4 cut(s) 2, 215, 1946, 2189
MmeI TCCRAC 8 cut(s) 221, 348, 1039, 1287, 1359, 1446, 1630, 2435
Mox20I TGGCCA 2 cut(s) 399, 2559
Mph1103I ATGCAT 2 cut(s) 1789, 2550
MroXI GAANNNNTTC 1 cut(s) 496
MscI TGGCCA 2 cut(s) 399, 2559
MseI TTAA 9 cut(s) 162, 168, 801, 1023, 1226, 1385, 1539, 1608, 2115
Msp20I TGGCCA 2 cut(s) 399, 2559
MspA1I CMGCKG 1 cut(s) 2465
MspCI CTTAAG 2 cut(s) 1225, 1538
MspI CCGG 5 cut(s) 267, 313, 1217, 1350, 2139
MspR9I CCNGG 6 cut(s) 268, 1217, 1290, 2295, 2346, 2484
MunI CAATTG 1 cut(s) 1169
Mva1269I GAATGC 3 cut(s) 1069, 1787, 1900
MvaI CCWGG 4 cut(s) 1290, 2295, 2346, 2484
MwoI GCNNNNNNNGC 3 cut(s) 309, 1294, 1467
NciI CCSGG 2 cut(s) 268, 1217
NdeI CATATG 1 cut(s) 1457
NdeII GATC 8 cut(s) 949, 1105, 1153, 1327, 1361, 1664, 1924, 2081
NlaIV GGNNCC 6 cut(s) 99, 685, 1287, 1420, 1513, 2237
NmuCI GTSAC 3 cut(s) 224, 1620, 2181
NsiI ATGCAT 2 cut(s) 1789, 2550
PaeR7I CTCGAG 1 cut(s) 2177
PciSI GCTCTTC 2 cut(s) 2332, 2365
PcsI WCGNNNNNNNCGW 1 cut(s) 1573
PctI GAATGC 3 cut(s) 1069, 1787, 1900
PdmI GAANNNNTTC 1 cut(s) 496
PflFI GACNNNGTC 1 cut(s) 205
PflMI CCANNNNNTGG 1 cut(s) 557
PfoI TCCNGGA 1 cut(s) 1215
PinAI ACCGGT 1 cut(s) 1349
PkrI GCNGC 5 cut(s) 302, 1202, 1761, 2464, 2467
PleI GAGTC 4 cut(s) 2, 214, 1946, 2188
PmaCI CACGTG 1 cut(s) 252
PmlI CACGTG 1 cut(s) 252
PpsI GAGTC 4 cut(s) 2, 214, 1946, 2188
Ppu21I YACGTR 1 cut(s) 252
PpuMI RGGWCCY 1 cut(s) 943
PshAI GACNNNNGTC 1 cut(s) 291
PshBI ATTAAT 1 cut(s) 801
PsiI TTATAA 1 cut(s) 932
Psp124BI GAGCTC 4 cut(s) 150, 707, 1224, 2178
Psp5II RGGWCCY 1 cut(s) 943
Psp6I CCWGG 4 cut(s) 1288, 2293, 2344, 2482
PspCI CACGTG 1 cut(s) 252
PspGI CCWGG 4 cut(s) 1288, 2293, 2344, 2482
PspN4I GGNNCC 6 cut(s) 99, 685, 1287, 1420, 1513, 2237
PspPI GGNCC 5 cut(s) 902, 943, 974, 1796, 2480
PspPPI RGGWCCY 1 cut(s) 943
PspXI VCTCGAGB 1 cut(s) 2177
PsrI GAACNNNNNNTAC 2 cut(s) 2228, 2260
PstNI CAGNNNCTG 1 cut(s) 228
PsuI RGATCY 3 cut(s) 1105, 1664, 2081
PsyI GACNNNGTC 1 cut(s) 205
PvuII CAGCTG 1 cut(s) 2465
RsaI GTAC 8 cut(s) 685, 725, 1187, 1409, 1546, 2016, 2246, 2292
RsaNI GTAC 8 cut(s) 684, 724, 1186, 1408, 1545, 2015, 2245, 2291
Rsr2I CGGWCCG 1 cut(s) 1796
RsrII CGGWCCG 1 cut(s) 1796
SacI GAGCTC 4 cut(s) 150, 707, 1224, 2178
SalI GTCGAC 1 cut(s) 2313
SapI GCTCTTC 2 cut(s) 2332, 2365
SaqAI TTAA 9 cut(s) 162, 168, 801, 1023, 1226, 1385, 1539, 1608, 2115
SatI GCNGC 5 cut(s) 301, 1201, 1760, 2463, 2466
Sau3AI GATC 8 cut(s) 949, 1105, 1153, 1327, 1361, 1664, 1924, 2081
Sau96I GGNCC 5 cut(s) 902, 943, 974, 1796, 2480
ScaI AGTACT 3 cut(s) 725, 1409, 1546
SchI GAGTC 4 cut(s) 2, 215, 1946, 2189
ScrFI CCNGG 6 cut(s) 268, 1217, 1290, 2295, 2346, 2484
SduI GDGCHC 4 cut(s) 150, 707, 1224, 2178
SexAI ACCWGGT 1 cut(s) 2482
SfaNI GCATC 5 cut(s) 124, 1090, 1187, 1212, 2028
Sfr274I CTCGAG 1 cut(s) 2177
SinI GGWCC 3 cut(s) 943, 1796, 2480
SlaI CTCGAG 1 cut(s) 2177
SmlI CTYRAG 8 cut(s) 149, 278, 833, 1004, 1225, 1538, 1932, 2177
SmoI CTYRAG 8 cut(s) 149, 278, 833, 1004, 1225, 1538, 1932, 2177
SpeI ACTAGT 1 cut(s) 1645
SsiI CCGC 4 cut(s) 667, 1200, 2402, 2417
SspMI CTAG 5 cut(s) 284, 1253, 1646, 1662, 2288
SstI GAGCTC 4 cut(s) 150, 707, 1224, 2178
StyD4I CCNGG 6 cut(s) 266, 1215, 1288, 2293, 2344, 2482
StyI CCWWGG 2 cut(s) 970, 1595
TaaI ACNGT 5 cut(s) 224, 230, 245, 343, 871
TaiI ACGT 3 cut(s) 254, 797, 2193
TaqI TCGA 9 cut(s) 123, 183, 372, 444, 516, 1960, 2043, 2178, 2314
TaqII GACCGA 4 cut(s) 1622, 1813, 1917, 2078
TatI WGTACW 4 cut(s) 723, 1185, 1407, 1544
TauI GCSGC 1 cut(s) 1203
Tru1I TTAA 9 cut(s) 162, 168, 801, 1023, 1226, 1385, 1539, 1608, 2115
Tru9I TTAA 9 cut(s) 162, 168, 801, 1023, 1226, 1385, 1539, 1608, 2115
TscAI CASTG 7 cut(s) 229, 400, 472, 1213, 1864, 1983, 2190
TseFI GTSAC 3 cut(s) 224, 1620, 2181
TseI GCWGC 4 cut(s) 300, 1759, 2462, 2465
Tsp45I GTSAC 3 cut(s) 224, 1620, 2181
TspGWI ACGGA 4 cut(s) 218, 1382, 1664, 1809
TspRI CASTG 7 cut(s) 229, 400, 472, 1213, 1864, 1983, 2190
Tth111I GACNNNGTC 1 cut(s) 205
Van91I CCANNNNNTGG 1 cut(s) 557
Vha464I CTTAAG 2 cut(s) 1225, 1538
VpaK11BI GGWCC 3 cut(s) 943, 1796, 2480
VspI ATTAAT 1 cut(s) 801
XapI RAATTY 3 cut(s) 926, 1140, 2217
XbaI TCTAGA 1 cut(s) 1252
XhoI CTCGAG 1 cut(s) 2177
XmiI GTMKAC 1 cut(s) 2314
XmnI GAANNNNTTC 1 cut(s) 496
XspI CTAG 5 cut(s) 284, 1253, 1646, 1662, 2288
ZraI GACGTC 1 cut(s) 795
ZrmI AGTACT 3 cut(s) 725, 1409, 1546
Zsp2I ATGCAT 2 cut(s) 1789, 2550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.