AT3G14780

Callose synthase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0023564
Physical Location & Seq
Reverse (-)
4961777 .. 4963569
1793 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G14780.1

Sequence Viewer

Length: 1044 bp
ATGTCGTCTAACGAAATCGCCCCTGTGGATCCTACCGATGTTTTTCTTGATATGGATCCCATCCTCCCGGAAGATTCGCCGGCAACCAGCTTGTTCACGAGACCCCTCGCTTTGAAAGAACAATATGTGTGGGAGCCCTATGACAGCGAGAAGCTTCCGGAGACATTAGCCTCAGGGATTCAAAGGTTTCTTCGTGTAGCAAACTTGGTTGAGTCTGATGATCCTCGTGTTGCTTATCTATGCCGTTTTTATACTTTTGAAGAAGCTCACCGCATTGACAGTAGATCTAACGGAAGAGGTGTACGACAGTTCAAAAATTCTCTTCTTCGAAGGCTAGAGAAGGATGACGAGTTTACTATAAGAAGAAGAAAGGAAATAAATGATCATAAGGAACTCAAACGTGTTTATCATGCTTACAATGAATACATCATCAGACATGGTGCATCATTCAATTTGGATAATAGTCAGCAAGAGAAGTTGATAAATGCACGCAGCATTGCTTCTGTTTTATATGAAATTCTAAGGAAAATCGAATCAAGTATGGGCCGTGCCTCAACCCCAGAAAGTATACAATTAAACCGGGATGAGGAGATTGGTGAGCTCCTTGATATGGACACCTCAATGGAGGAGACCAACAAGATAAATTCGGACGAGACCTTTGTCGACCAAAAAGCCGAGTTGGTAGTTGAGAAGAGCAAAGTCTTAGAGGACGAGAGACTAACTCAAATGTTTCAAGGAGACCACCAATATCTCACCCAAGATGAAAAAGATGACATATACAAAGAGGAGGTTCAAATAAAGAAAGACAAACGTTTTGCATATGGATCAATGAAGGCGGTTCCATCTTTGTTTTACGAAGATAAGTATCTCGAGACACAAGAAAAGTTGAACAAGCTGGAGAAGAAGATTGAAGAGATGGAGCTGAAACTCAAGGATGTCGACTTTTGGACTAACATGATGCAAAACATGTTTCCAGACCAAGTACCTCCATCTATGAGAGCAAACCAAACCGATAACAACAAAAACGACAAACTAAGCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

347

Amino Acids

40.8

Weight (kDa)

4.95

Isoelectric Point (pI)

46.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 60 - 176 1.1e-08 Vta1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023564)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14780
malus_domestica MD09G1150100.v1.1
pyrus_communis pycom09g06950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 568, 663, 939
AccIII TCCGGA 1 cut(s) 157
AciI CCGC 2 cut(s) 271, 836
AclI AACGTT 1 cut(s) 811
AclWI GGATC 6 cut(s) 23, 36, 50, 63, 215, 832
AcsI RAATTY 3 cut(s) 316, 516, 643
AfaI GTAC 2 cut(s) 303, 984
AfiI CCNNNNNNNGG 2 cut(s) 586, 610
AflIII ACRYGT 2 cut(s) 400, 966
AgsI TTSAA 9 cut(s) 115, 182, 260, 313, 451, 734, 794, 889, 911
AluBI AGCT 6 cut(s) 90, 154, 266, 601, 895, 922
AluI AGCT 6 cut(s) 90, 154, 266, 601, 895, 922
Alw21I GWGCWC 1 cut(s) 603
Alw26I GTCTC 7 cut(s) 94, 155, 623, 647, 709, 732, 866
AlwI GGATC 6 cut(s) 23, 36, 50, 63, 215, 832
Ama87I CYCGRG 1 cut(s) 869
Aor13HI TCCGGA 1 cut(s) 157
AoxI GGCC 1 cut(s) 544
ApeKI GCWGC 1 cut(s) 492
ApoI RAATTY 3 cut(s) 316, 516, 643
ArsI GACNNNNNNTTYG 4 cut(s) 641, 673, 797, 829
AspS9I GGNCC 1 cut(s) 544
AsuC2I CCSGG 2 cut(s) 68, 581
AsuHPI GGTGA 3 cut(s) 260, 608, 745
AsuII TTCGAA 1 cut(s) 328
AvaI CYCGRG 1 cut(s) 869
AxyI CCTNAGG 1 cut(s) 172
BamHI GGATCC 2 cut(s) 28, 55
BanII GRGCYC 2 cut(s) 138, 603
BauI CACGAG 2 cut(s) 97, 225
Bbv12I GWGCWC 1 cut(s) 603
BbvI GCAGC 1 cut(s) 504
BccI CCATC 4 cut(s) 68, 850, 910, 997
BceAI ACGGC 2 cut(s) 228, 531
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 2 cut(s) 68, 581
BcoDI GTCTC 7 cut(s) 94, 155, 623, 647, 709, 732, 866
BfaI CTAG 1 cut(s) 335
BglII AGATCT 1 cut(s) 284
BisI GCNGC 1 cut(s) 493
BlsI GCNGC 1 cut(s) 494
Bme1390I CCNGG 2 cut(s) 68, 581
BmeT110I CYCGRG 1 cut(s) 869
BmgT120I GGNCC 1 cut(s) 544
BmiI GGNNCC 4 cut(s) 30, 57, 135, 840
BmrFI CCNGG 2 cut(s) 68, 581
BmsI GCATC 2 cut(s) 452, 948
BoxI GACNNNNGTC 1 cut(s) 659
BplI GAGNNNNNCTC 2 cut(s) 706, 738
BpmI CTGGAG 1 cut(s) 917
Bpu14I TTCGAA 1 cut(s) 328
BpuEI CTTGAG 1 cut(s) 914
BpuMI CCSGG 2 cut(s) 68, 581
BsaBI GATNNNNATC 2 cut(s) 54, 864
BsaI GGTCTC 4 cut(s) 94, 623, 647, 732
BsaWI WCCGGW 1 cut(s) 157
Bsc4I CCNNNNNNNGG 2 cut(s) 586, 610
Bse118I RCCGGY 1 cut(s) 79
Bse21I CCTNAGG 1 cut(s) 172
Bse3DI GCAATG 1 cut(s) 495
Bse8I GATNNNNATC 2 cut(s) 54, 864
BseAI TCCGGA 1 cut(s) 157
BseGI GGATG 4 cut(s) 60, 349, 589, 940
BseJI GATNNNNATC 2 cut(s) 54, 864
BseLI CCNNNNNNNGG 2 cut(s) 586, 610
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 1 cut(s) 186
BseRI GAGGAG 3 cut(s) 602, 641, 800
BseXI GCAGC 1 cut(s) 504
BshFI GGCC 1 cut(s) 546
BsiHKAI GWGCWC 1 cut(s) 603
BsiHKCI CYCGRG 1 cut(s) 869
BsiSI CCGG 4 cut(s) 68, 80, 158, 580
BslI CCNNNNNNNGG 2 cut(s) 586, 610
BsmAI GTCTC 7 cut(s) 94, 155, 623, 647, 709, 732, 866
BsnI GGCC 1 cut(s) 546
Bso31I GGTCTC 4 cut(s) 94, 623, 647, 732
BsoBI CYCGRG 1 cut(s) 869
Bsp119I TTCGAA 1 cut(s) 328
Bsp1286I GDGCHC 2 cut(s) 138, 603
Bsp13I TCCGGA 1 cut(s) 157
Bsp143I GATC 6 cut(s) 28, 55, 220, 284, 382, 824
BspACI CCGC 2 cut(s) 271, 836
BspANI GGCC 1 cut(s) 546
BspCNI CTCAG 1 cut(s) 185
BspEI TCCGGA 1 cut(s) 157
BspLI GGNNCC 4 cut(s) 30, 57, 135, 840
BspPI GGATC 6 cut(s) 23, 36, 50, 63, 215, 832
BspQI GCTCTTC 1 cut(s) 686
BspT104I TTCGAA 1 cut(s) 328
BspTNI GGTCTC 4 cut(s) 94, 623, 647, 732
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 79
BssAI RCCGGY 1 cut(s) 79
BssMI GATC 6 cut(s) 28, 55, 220, 284, 382, 824
BssNAI GTATAC 1 cut(s) 569
BssSI CACGAG 2 cut(s) 97, 225
Bst1107I GTATAC 1 cut(s) 569
Bst2BI CACGAG 2 cut(s) 97, 225
Bst4CI ACNGT 2 cut(s) 281, 309
Bst6I CTCTTC 4 cut(s) 289, 327, 686, 906
BstBI TTCGAA 1 cut(s) 328
BstC8I GCNNGC 2 cut(s) 81, 490
BstDEI CTNAG 4 cut(s) 172, 521, 703, 1034
BstF5I GGATG 4 cut(s) 60, 349, 589, 940
BstKTI GATC 6 cut(s) 31, 58, 223, 287, 385, 827
BstMAI GTCTC 7 cut(s) 94, 155, 623, 647, 709, 732, 866
BstMBI GATC 6 cut(s) 28, 55, 220, 284, 382, 824
BstNSI RCATGY 1 cut(s) 970
BstPAI GACNNNNGTC 1 cut(s) 659
BstSCI CCNGG 2 cut(s) 66, 579
BstV1I GCAGC 1 cut(s) 504
BstX2I RGATCY 3 cut(s) 28, 55, 284
BstYI RGATCY 3 cut(s) 28, 55, 284
BstZ17I GTATAC 1 cut(s) 569
Bsu36I CCTNAGG 1 cut(s) 172
BsuRI GGCC 1 cut(s) 546
BtsCI GGATG 4 cut(s) 60, 349, 589, 940
Cac8I GCNNGC 2 cut(s) 81, 490
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 544
Csp6I GTAC 2 cut(s) 302, 983
CspCI CAANNNNNGTGG 2 cut(s) 110, 145
CviAII CATG 4 cut(s) 410, 437, 955, 967
CviQI GTAC 2 cut(s) 302, 983
DdeI CTNAG 4 cut(s) 172, 521, 703, 1034
DpnI GATC 6 cut(s) 30, 57, 222, 286, 384, 826
DpnII GATC 6 cut(s) 28, 55, 220, 284, 382, 824
Eam1104I CTCTTC 4 cut(s) 289, 327, 686, 906
EarI CTCTTC 4 cut(s) 289, 327, 686, 906
Ecl136II GAGCTC 1 cut(s) 601
Eco24I GRGCYC 2 cut(s) 138, 603
Eco31I GGTCTC 4 cut(s) 94, 623, 647, 732
Eco53kI GAGCTC 1 cut(s) 601
Eco81I CCTNAGG 1 cut(s) 172
Eco88I CYCGRG 1 cut(s) 869
EcoICRI GAGCTC 1 cut(s) 601
EcoT38I GRGCYC 2 cut(s) 138, 603
FaeI CATG 4 cut(s) 413, 440, 958, 970
FatI CATG 4 cut(s) 409, 436, 954, 966
FauNDI CATATG 1 cut(s) 820
FbaI TGATCA 1 cut(s) 382
FblI GTMKAC 3 cut(s) 568, 663, 939
Fnu4HI GCNGC 1 cut(s) 493
FokI GGATG 4 cut(s) 47, 356, 596, 947
FriOI GRGCYC 2 cut(s) 138, 603
Fsp4HI GCNGC 1 cut(s) 493
FspBI CTAG 1 cut(s) 335
GluI GCNGC 1 cut(s) 493
GsuI CTGGAG 1 cut(s) 917
HaeIII GGCC 1 cut(s) 546
HapII CCGG 4 cut(s) 68, 80, 158, 580
Hin1II CATG 4 cut(s) 413, 440, 958, 970
HincII GTYRAC 2 cut(s) 664, 940
HindII GTYRAC 2 cut(s) 664, 940
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 4 cut(s) 74, 178, 212, 533
HpaII CCGG 4 cut(s) 68, 80, 158, 580
HphI GGTGA 3 cut(s) 260, 608, 745
Hpy166II GTNNAC 6 cut(s) 96, 302, 354, 569, 664, 940
Hpy188I TCNGA 3 cut(s) 217, 434, 649
Hpy188III TCNNGA 6 cut(s) 47, 97, 158, 869, 871, 974
Hpy8I GTNNAC 6 cut(s) 96, 302, 354, 569, 664, 940
HpyAV CCTTC 3 cut(s) 324, 334, 826
HpyCH4III ACNGT 2 cut(s) 281, 309
HpyCH4IV ACGT 2 cut(s) 400, 811
HpyCH4V TGCA 4 cut(s) 443, 488, 818, 961
HpyF3I CTNAG 4 cut(s) 172, 521, 703, 1034
HpySE526I ACGT 2 cut(s) 400, 811
Hsp92II CATG 4 cut(s) 413, 440, 958, 970
Kpn2I TCCGGA 1 cut(s) 157
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 6 cut(s) 28, 55, 220, 284, 382, 824
LguI GCTCTTC 1 cut(s) 686
LmnI GCTCC 3 cut(s) 133, 606, 919
Lsp1109I GCAGC 1 cut(s) 504
LweI GCATC 2 cut(s) 452, 948
MaeI CTAG 1 cut(s) 335
MaeII ACGT 2 cut(s) 400, 811
MalI GATC 6 cut(s) 30, 57, 222, 286, 384, 826
MboI GATC 6 cut(s) 28, 55, 220, 284, 382, 824
MflI RGATCY 3 cut(s) 28, 55, 284
MhlI GDGCHC 2 cut(s) 138, 603
MluCI AATT 5 cut(s) 316, 451, 516, 572, 643
MlyI GAGTC 1 cut(s) 221
MroI TCCGGA 1 cut(s) 157
MroNI GCCGGC 1 cut(s) 79
MseI TTAA 2 cut(s) 575, 1042
MslI CAYNNNNRTG 1 cut(s) 620
MspI CCGG 4 cut(s) 68, 80, 158, 580
MspR9I CCNGG 2 cut(s) 68, 581
NaeI GCCGGC 1 cut(s) 81
NciI CCSGG 2 cut(s) 68, 581
NdeI CATATG 1 cut(s) 820
NdeII GATC 6 cut(s) 28, 55, 220, 284, 382, 824
NgoMIV GCCGGC 1 cut(s) 79
NlaIII CATG 4 cut(s) 413, 440, 958, 970
NlaIV GGNNCC 4 cut(s) 30, 57, 135, 840
NmeAIII GCCGAG 1 cut(s) 700
NspI RCATGY 1 cut(s) 970
NspV TTCGAA 1 cut(s) 328
PaeR7I CTCGAG 1 cut(s) 869
PciI ACATGT 1 cut(s) 966
PciSI GCTCTTC 1 cut(s) 686
PdiI GCCGGC 1 cut(s) 81
PfeI GAWTC 3 cut(s) 74, 178, 533
PfoI TCCNGGA 1 cut(s) 66
PkrI GCNGC 1 cut(s) 494
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
PscI ACATGT 1 cut(s) 966
PshAI GACNNNNGTC 1 cut(s) 659
Psp124BI GAGCTC 1 cut(s) 603
Psp1406I AACGTT 1 cut(s) 811
PspN4I GGNNCC 4 cut(s) 30, 57, 135, 840
PspPI GGNCC 1 cut(s) 544
PsuI RGATCY 3 cut(s) 28, 55, 284
RsaI GTAC 2 cut(s) 303, 984
RsaNI GTAC 2 cut(s) 302, 983
RseI CAYNNNNRTG 1 cut(s) 620
SacI GAGCTC 1 cut(s) 603
SalI GTCGAC 2 cut(s) 662, 938
SapI GCTCTTC 1 cut(s) 686
SaqAI TTAA 2 cut(s) 575, 1042
SatI GCNGC 1 cut(s) 493
Sau3AI GATC 6 cut(s) 28, 55, 220, 284, 382, 824
Sau96I GGNCC 1 cut(s) 544
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 2 cut(s) 68, 581
SduI GDGCHC 2 cut(s) 138, 603
SfaNI GCATC 2 cut(s) 452, 948
Sfr274I CTCGAG 1 cut(s) 869
SfuI TTCGAA 1 cut(s) 328
SlaI CTCGAG 1 cut(s) 869
SmiMI CAYNNNNRTG 1 cut(s) 620
SmlI CTYRAG 2 cut(s) 869, 929
SmoI CTYRAG 2 cut(s) 869, 929
Sse9I AATT 5 cut(s) 316, 451, 516, 572, 643
SsiI CCGC 2 cut(s) 271, 836
SspMI CTAG 1 cut(s) 335
SstI GAGCTC 1 cut(s) 603
StyD4I CCNGG 2 cut(s) 66, 579
TaaI ACNGT 2 cut(s) 281, 309
TaiI ACGT 2 cut(s) 403, 814
TaqI TCGA 5 cut(s) 328, 531, 663, 870, 939
TasI AATT 5 cut(s) 316, 451, 516, 572, 643
TfiI GAWTC 3 cut(s) 74, 178, 533
Tru1I TTAA 2 cut(s) 575, 1042
Tru9I TTAA 2 cut(s) 575, 1042
TseI GCWGC 1 cut(s) 492
TspDTI ATGAA 4 cut(s) 435, 528, 777, 845
TspGWI ACGGA 1 cut(s) 306
XapI RAATTY 3 cut(s) 316, 516, 643
XceI RCATGY 1 cut(s) 970
XhoI CTCGAG 1 cut(s) 869
XmiI GTMKAC 3 cut(s) 568, 663, 939
XspI CTAG 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.