Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0023564

Physical Location & Seq

Genomic Coordinates Forward (+)

11823248 .. 11834892

Gene Structure 11645 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1150100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3663 bp

ATGGCGAGCTCCAGCGGTACGAAGAACAAGTTCCGGGGGCCGTCTCGGTCATTTTCGAGGGCAATGACGAGAATGCCCACAAGGGTTTTGGAGTCGCCGAACGAGGACGAGTCGTTGGACAGCATAGTTGTGCCCCCGGGACTTCCCTCAATTGCTCGGATTTTTCGAGTTGCGGATGAGATTGAGAAGGACAACCCTAGGGTTGCTTATCTCTGCCGCTTCCATGGATTTGAGAAGGCACACAAGATGGATCCAACATCAAGTGGTCGTGGTTTTCGACAGTTTAAGACACTTCTTTTGAACAAACTTGACGAAATAGTGACAAAACATCAGCTTGCTAAATCTGATCCACAAGAAATTGTGAAGTATTACCACCAATTCTATGAGAAAAATATCAAAGATGGAGAGTACACAAAGAAACCGGAAGAGATGGCTAAGAATTGTCAGATTGCAACAGTACTGTATGATGTGCTCATGACAGTGGTACCTCAAACCAAAATAGACAAACAGACACTGAAGATGGCTGAAGATGTCAAGAAGAAAAGGGAGCAGTTTGTCAACTATAACATTCTTCCGCTGTATGGTGGTGGTGTTAAACCAGCAATTATGGAACTTCCCAAGGTTGTACATCAAAGCAGCGCTCCGTGCTCTACAAAATGTGAACAGTCTTCCAATGCCAAAATTTCATGTGAAGCTCCACGAATCCTGATGACAAGTCAACAGTGCCTACAGAGAGGATTAAACCTGTCAATGATATACTCGATTGGCTTTCTTCTATTTTCGGGTTTCAGGTCCAAAACTTTTATGGGAAATGTGGCAAATCAGAGGGAACACTTACTCTTGCTACTTGCCAACATGGACGTAAGACATAGAAATCTTGAAAATTTCACTGAGTTGAACAGTGCCACTGTACAACAATTGAGTGAGAAAATTTTTAAGAATTATCGTTCCTGGTTTAACTATTTGCATTACAACAATTGGAACTTATTTACATTGCGCTCTATCTTCTCATATGGGGTGAAGCTTCAAATATTCGGTTCATGCCTGAATGCTTATGCTATATTTTCCATCAGATGGCCAAAGAGGTATATGGAATTTTATCTCGCAATGCTCATCCGGCCTACTGGAGAAATATACGAAACAACAGCACGGGATGAAGAATCTTTTCTGAGGGATGTTGTAACTCCTATCTACCAGGTCTTGTACAAGCAAAGAGAAACAAAAATGGCAAGGCAAGTCATTCAAGATGGAGAAATTATGATGATCTTAAATGAATACTTTTGGTCCGACAGATGTTTTAGGCTAGGGTGGCCAATGGACCCTAAAGCAGATTTTTTTAGACATTCAGATGGGATTCAACCAACAAAGGAAGCTGCCGGTGGGAGGAGGAAACCTAAAACAAATTTTGTAGAAGTTCGAACATTTTGGCACCTCTACAGAAGTTTTGATCGAATGTGGATATTCTTGATATTGGCTTTTCAGGCTATGCTTATTGTTGCATGGAGTACTTCTGGTTCTCTGACTGCTTTCTTTGATGCGGATGTCTTCAGAAGTGTTTTAAGCATCTTCATCACTTATGCTTTCCTCAACTTATTACAAGCCACTCTGGATATAATTCTTAGTTGGCATTGTTGGAAGAGCTTGAAATTCACTCAAATACTACGATACCTCTTGAAATTTTCTGTAGCTGGTGTATGGGCTGTGGTTCTGCCCATTGGTTATTCTAGTTCTGTGCAGAGTCCTACAGGACTTCTAAAATTCTTCAACAGTTGGGCTAGGGATTGGCGGAATCAATCATTTTATAATTATGCTGTTGCGCTCTATCTGCTTCCTAATATAATGGCTGCTGTGCTATTATTTCTTCCACCTTTACGGAGGCACCTAGAGCGCTCAAACTGGAGGATTGTCACTCTTTTTATGTGGTGGGCTCAGGCAAGCATGTCTGAAACAAATTTCTTGTTGCCTTGTGGTTTAGATAAATGTTTTTGCTTTCCAAAACTGTATATAGGAAGAGGGCTTCATGAGGACTTCTTTTCACTGCTGAAATATACGCTGTTTTGGATCGTTCTGCTAATCAGCAAGCTATCGTTCAGCTATTATGTGGAGATACTGCCACTTGTTGCACCAACAAAAATAATAATGAAGATGCCTATAAGTAATTATCAATGGCATGAGTTCTTTCCAAATGTAACTCATAATATTGGCGTTGTTATTGCAATATGGGCTCCAATTGTCCTGGTTTATTTTATGGATGCTCAAATATGGTATGCAATATTTTCTACTATCTTTGGTGGAATTCATGGAGCCTTCAGCCATTTGGGTGAGATACGGACCCTTGGGATGTTGAGGTCCAGATTTGAGTCTCTGCCTTCTGCTTTCAATGATCGTCTTATGCCGTCACAAAATGAGGATGCAAACGAGGAAGGACCATTGATGAATGAGGAACGCCAACGAAAGAACATTGATGAGGAACGCCAACGAAAGAACATTGCTAATTTCTCTCAGGTGTGGAATGAGTTCATAACTTCAATGCGATGGGAGGACCTTATCAGCAATAGAGATAGAGATCTGCTGCTTGTTCCATATTCGTCAAGTGATCTGTCTGTTGTCCAGTGGCCTCCTTTCTTGCTTGCTAGTAAGATTCCGATAGCATTGGACATGGCAAAAGATTTTACGGGGAAGGCAGATGATGATTTATTTAAAAAGATTAAGAGTGATGATTGTATGTACTCAGCAGTAATTGAATGCTATCAGACACTCAGTGATATTATATATGGCCTTTTGGAAGATGAAGCAGATAAGATGATCGTAAAGTGGATATGCAATGAAGTCGACAGTAGTATAAACCAGAAAAGATTTTTGACTAAATTCCGCATGAGTGGGCTGCTATTTCTTAGCGAGAGGTTGGAGAAATTTCTAGAGCTCTTGATGAGTAATGATGATAATTCCATGCGTCAGATAATCAATGTCCTTCAAGATATCATGGAGATTATCACACAGGATGTTATGATCAACGGCCATGAAATACTGGAGGCAGCACATATACATTTGATTCCTCAAAATGTCAAGAAGGAGCAAAGATTTCAAAAGCTTAAGATTGACCAGAGACATAAGGGTTGGAGGGAGAAGGTAGTCAGACTTCATTTGCTTTTGACCGTCAAGGAGTCTGCCATCAATGTCCCTCAAAACTTGGATGCTCGCAGGCGCATCACCTTCTTTGCAAACTCCTTATTCATGAACATGCCACGTGCTCCCAAAGTTCGTGACATGCTCTCATTCAGTGTTCTGACTCCTTATTACAAAGAAGATGTCATCTATTCTGATGAGGAACTTAATAAAGAAAATGAGGATGGAATCTCAGTTTTGTTCTACCTGCAGAAAATATATCCTGAGTTAACCCGTCAGTGGGTATCTTACAGGGCACAGACACTTTCTAGAACAGTGAGAGGGATGATGTACTATAGGAGGGCTTTAGACCTTCAGTGTGTCCTGGAAACAGCAGGAGATAGTGATTTTTTGGGAGGCTACCAAACCTCAGGGTTAAGCGAAAATGATGAGCAGGCTGAGCAGGCTTTTCTAGATCGTGCACAAGCTCTGGCAGATTTGAAGTTTACATATGTAGTTTCTTGTCAGATCTATGGTGCTCAGAAAAACTCCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003843 GO:0006810 GO:0008150 GO:0008194 GO:0010232 GO:0010233 GO:0016740 GO:0016757 GO:0016758 GO:0032501 GO:0033036 GO:0033037 GO:0035251 GO:0046527 GO:0051179 GO:0051234 GO:0052545 GO:0080165

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1221

Amino Acids

142.1

Weight (kDa)

8.85

Isoelectric Point (pI)

47.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vta1	PF04652	50 - 178	2.1e-08	Vta1 like
FKS1_dom1	PF14288	378 - 438	2.1e-06	1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1	PF25968	873 - 1053	7.6e-65	Callose synthase 1 domain
Glucan_synthase	PF02364	1060 - 1131	1.2e-23	1,3-beta-glucan synthase component
Glucan_synthase	PF02364	1133 - 1219	3.2e-15	1,3-beta-glucan synthase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0023564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14780
malus_domestica	MD09G1150100.v1.1
pyrus_communis	pycom09g06950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1803
Acc36I	ACCTGC	1 cut(s)	3381
Acc65I	GGTACC	1 cut(s)	484
AccB1I	GGYRCC	3 cut(s)	484, 1428, 1878
AccB7I	CCANNNNNTGG	1 cut(s)	1074
AccI	GTMKAC	1 cut(s)	2832
AciI	CCGC	7 cut(s)	15, 173, 217, 575, 1538, 1786, 2872
AclWI	GGATC	4 cut(s)	245, 258, 341, 2069
AcoI	YGGCCR	3 cut(s)	1076, 1310, 3016
AcuI	CTGAAG	5 cut(s)	536, 546, 1531, 2293, 3464
AcvI	CACGTG	1 cut(s)	3248
AdeI	CACNNNGTG	1 cut(s)	2762
AfeI	AGCGCT	2 cut(s)	640, 1889
AfiI	CCNNNNNNNGG	5 cut(s)	581, 1074, 1383, 3405, 3406
AflII	CTTAAG	1 cut(s)	3092
AjnI	CCWGG	4 cut(s)	950, 1194, 2235, 3489
Alw21I	GWGCWC	7 cut(s)	11, 474, 650, 2925, 3253, 3589, 3646
Alw26I	GTCTC	3 cut(s)	48, 2367, 3100
Alw44I	GTGCAC	1 cut(s)	3585
AlwI	GGATC	4 cut(s)	245, 258, 341, 2069
AlwNI	CAGNNNCTG	1 cut(s)	514
Ama87I	CYCGRG	1 cut(s)	136
Aor51HI	AGCGCT	2 cut(s)	640, 1889
AoxI	GGCC	7 cut(s)	38, 1076, 1118, 1310, 2615, 2776, 3016
ApaLI	GTGCAC	1 cut(s)	3585
ApeKI	GCWGC	6 cut(s)	636, 1373, 1844, 2572, 2884, 3035
ArsI	GACNNNNNNTTYG	4 cut(s)	280, 312, 3294, 3326
Asp700I	GAANNNNTTC	3 cut(s)	29, 1164, 2516
Asp718I	GGTACC	1 cut(s)	484
AspA2I	CCTAGG	1 cut(s)	197
AspLEI	GCGC	5 cut(s)	641, 999, 1819, 1890, 3207
AspS9I	GGNCC	8 cut(s)	38, 792, 1284, 1318, 2331, 2349, 2426, 2542
AsuC2I	CCSGG	3 cut(s)	35, 137, 138
AsuHPI	GGTGA	3 cut(s)	1030, 2333, 3202
AsuII	TTCGAA	1 cut(s)	1417
AvaI	CYCGRG	1 cut(s)	136
AvaII	GGWCC	7 cut(s)	792, 1284, 1318, 2331, 2349, 2426, 2542
AvrII	CCTAGG	1 cut(s)	197
AxyI	CCTNAGG	1 cut(s)	3535
BaeGI	GKGCMC	3 cut(s)	135, 3424, 3589
BaeI	ACNNNNGTAYC	2 cut(s)	468, 501
BalI	TGGCCA	2 cut(s)	1078, 1312
BamHI	GGATCC	1 cut(s)	250
BanI	GGYRCC	3 cut(s)	484, 1428, 1878
BanII	GRGCYC	4 cut(s)	11, 1930, 2227, 2925
BarI	GAAGNNNNNNTAC	2 cut(s)	3123, 3155
BbrPI	CACGTG	1 cut(s)	3248
BbsI	GAAGAC	2 cut(s)	660, 1537
Bbv12I	GWGCWC	7 cut(s)	11, 474, 650, 2925, 3253, 3589, 3646
BbvI	GCAGC	6 cut(s)	648, 1360, 1831, 2559, 2871, 3047
BceAI	ACGGC	3 cut(s)	25, 2380, 3031
BcgI	CGANNNNNNTGC	2 cut(s)	2812, 2846
BciT130I	CCWGG	4 cut(s)	952, 1196, 2237, 3491
BclI	TGATCA	1 cut(s)	3009
BcnI	CCSGG	3 cut(s)	35, 137, 138
BcoDI	GTCTC	3 cut(s)	48, 2367, 3100
BfaI	CTAG	9 cut(s)	198, 1304, 1725, 1776, 1883, 2634, 2918, 3435, 3578
BfmI	CTRYAG	6 cut(s)	728, 1435, 1683, 1743, 3374, 3460
BfoI	RGCGCY	2 cut(s)	642, 1891
BfrI	CTTAAG	1 cut(s)	3092
BfuAI	ACCTGC	1 cut(s)	3381
BglII	AGATCT	2 cut(s)	2566, 3633
BisI	GCNGC	7 cut(s)	217, 637, 1374, 1845, 2573, 2885, 3036
BlnI	CCTAGG	1 cut(s)	197
BlpI	GCTNAGC	1 cut(s)	3564
BlsI	GCNGC	7 cut(s)	218, 638, 1375, 1846, 2574, 2886, 3037
BmcAI	AGTACT	2 cut(s)	459, 1507
Bme1390I	CCNGG	7 cut(s)	35, 137, 138, 952, 1196, 2237, 3491
Bme18I	GGWCC	7 cut(s)	792, 1284, 1318, 2331, 2349, 2426, 2542
BmeT110I	CYCGRG	1 cut(s)	136
BmgT120I	GGNCC	8 cut(s)	38, 792, 1284, 1318, 2331, 2349, 2426, 2542
BmiI	GGNNCC	9 cut(s)	39, 252, 486, 1320, 1430, 1880, 2226, 2305, 2333
BmrFI	CCNGG	7 cut(s)	35, 137, 138, 952, 1196, 2237, 3491
BmsI	GCATC	7 cut(s)	1525, 1572, 2136, 2242, 2401, 3184, 3216
BpiI	GAAGAC	2 cut(s)	660, 1537
BpmI	CTGGAG	3 cut(s)	1146, 1918, 3050
Bpu10I	CCTNAGC	1 cut(s)	1929
Bpu1102I	GCTNAGC	1 cut(s)	3564
Bpu14I	TTCGAA	1 cut(s)	1417
BpuMI	CCSGG	3 cut(s)	35, 137, 138
BsaAI	YACGTR	1 cut(s)	3248
BsaBI	GATNNNNATC	1 cut(s)	2565
BsaJI	CCNNGG	7 cut(s)	34, 135, 136, 197, 223, 618, 2335
BsaWI	WCCGGW	1 cut(s)	421
Bsc4I	CCNNNNNNNGG	5 cut(s)	581, 1074, 1383, 3405, 3406
Bse118I	RCCGGY	1 cut(s)	1376
Bse1I	ACTGG	4 cut(s)	1129, 1901, 2611, 3033
Bse21I	CCTNAGG	1 cut(s)	3535
Bse3DI	GCAATG	5 cut(s)	69, 992, 1113, 2487, 2830
Bse8I	GATNNNNATC	1 cut(s)	2565
BseBI	CCWGG	4 cut(s)	952, 1196, 2237, 3491
BseDI	CCNNGG	7 cut(s)	34, 135, 136, 197, 223, 618, 2335
BseJI	GATNNNNATC	1 cut(s)	2565
BseLI	CCNNNNNNNGG	5 cut(s)	581, 1074, 1383, 3405, 3406
BseMI	GCAATG	5 cut(s)	69, 992, 1113, 2487, 2830
BseNI	ACTGG	4 cut(s)	1129, 1901, 2611, 3033
BseRI	GAGGAG	1 cut(s)	1399
BseSI	GKGCMC	3 cut(s)	135, 3424, 3589
BseXI	GCAGC	6 cut(s)	648, 1360, 1831, 2559, 2871, 3047
BsgI	GTGCAG	1 cut(s)	1754
BshFI	GGCC	7 cut(s)	40, 1078, 1120, 1312, 2617, 2778, 3018
BshNI	GGYRCC	3 cut(s)	484, 1428, 1878
BsiHKAI	GWGCWC	7 cut(s)	11, 474, 650, 2925, 3253, 3589, 3646
BsiHKCI	CYCGRG	1 cut(s)	136
BsiSI	CCGG	5 cut(s)	34, 137, 422, 1117, 1377
BslFI	GGGAC	2 cut(s)	153, 3164
BslI	CCNNNNNNNGG	5 cut(s)	581, 1074, 1383, 3405, 3406
BsmAI	GTCTC	3 cut(s)	48, 2367, 3100
BsmBI	CGTCTC	1 cut(s)	48
BsmFI	GGGAC	2 cut(s)	153, 3164
BsmI	GAATGC	3 cut(s)	78, 1054, 2750
BsnI	GGCC	7 cut(s)	40, 1078, 1120, 1312, 2617, 2778, 3018
BsoBI	CYCGRG	1 cut(s)	136
Bsp119I	TTCGAA	1 cut(s)	1417
Bsp1407I	TGTACA	3 cut(s)	625, 910, 1203
Bsp1720I	GCTNAGC	1 cut(s)	3564
Bsp19I	CCATGG	1 cut(s)	223
BspACI	CCGC	7 cut(s)	15, 173, 217, 575, 1538, 1786, 2872
BspANI	GGCC	7 cut(s)	40, 1078, 1120, 1312, 2617, 2778, 3018
BspHI	TCATGA	3 cut(s)	474, 2020, 3234
BspLI	GGNNCC	9 cut(s)	39, 252, 486, 1320, 1430, 1880, 2226, 2305, 2333
BspMAI	CTGCAG	1 cut(s)	3378
BspMI	ACCTGC	1 cut(s)	3381
BspPI	GGATC	4 cut(s)	245, 258, 341, 2069
BspQI	GCTCTTC	1 cut(s)	1631
BspT104I	TTCGAA	1 cut(s)	1417
BspT107I	GGYRCC	3 cut(s)	484, 1428, 1878
BspTI	CTTAAG	1 cut(s)	3092
BsrDI	GCAATG	5 cut(s)	69, 992, 1113, 2487, 2830
BsrFI	RCCGGY	1 cut(s)	1376
BsrGI	TGTACA	3 cut(s)	625, 910, 1203
BsrI	ACTGG	4 cut(s)	1129, 1901, 2611, 3033
BssAI	RCCGGY	1 cut(s)	1376
BssECI	CCNNGG	7 cut(s)	34, 135, 136, 197, 223, 618, 2335
BssT1I	CCWWGG	4 cut(s)	197, 223, 618, 2335
Bst2UI	CCWGG	4 cut(s)	952, 1196, 2237, 3491
Bst6I	CTCTTC	3 cut(s)	420, 1631, 2005
BstAFI	CTTAAG	1 cut(s)	3092
BstAUI	TGTACA	3 cut(s)	625, 910, 1203
BstBAI	YACGTR	1 cut(s)	3248
BstBI	TTCGAA	1 cut(s)	1417
BstC8I	GCNNGC	9 cut(s)	7, 336, 1936, 2081, 2631, 3199, 3203, 3561, 3570
BstDSI	CCRYGG	1 cut(s)	223
BstH2I	RGCGCY	2 cut(s)	642, 1891
BstHHI	GCGC	5 cut(s)	641, 999, 1819, 1890, 3207
BstMAI	GTCTC	3 cut(s)	48, 2367, 3100
BstMWI	GCNNNNNNNGC	9 cut(s)	645, 1117, 1309, 1481, 1825, 1885, 2222, 3565, 3569
BstNI	CCWGG	4 cut(s)	952, 1196, 2237, 3491
BstNSI	RCATGY	3 cut(s)	1942, 3244, 3271
BstSCI	CCNGG	7 cut(s)	33, 135, 136, 950, 1194, 2235, 3489
BstSFI	CTRYAG	6 cut(s)	728, 1435, 1683, 1743, 3374, 3460
BstSLI	GKGCMC	3 cut(s)	135, 3424, 3589
BstV1I	GCAGC	6 cut(s)	648, 1360, 1831, 2559, 2871, 3047
BstV2I	GAAGAC	2 cut(s)	660, 1537
BstX2I	RGATCY	3 cut(s)	250, 2566, 3633
BstYI	RGATCY	3 cut(s)	250, 2566, 3633
Bsu36I	CCTNAGG	1 cut(s)	3535
BsuRI	GGCC	7 cut(s)	40, 1078, 1120, 1312, 2617, 2778, 3018
BtgI	CCRYGG	1 cut(s)	223
BtgZI	GCGATG	1 cut(s)	2548
BtsI	GCAGTG	1 cut(s)	2036
BveI	ACCTGC	1 cut(s)	3381
Cac8I	GCNNGC	9 cut(s)	7, 336, 1936, 2081, 2631, 3199, 3203, 3561, 3570
CaiI	CAGNNNCTG	1 cut(s)	514
CciI	TCATGA	3 cut(s)	474, 2020, 3234
CfoI	GCGC	5 cut(s)	641, 999, 1819, 1890, 3207
Cfr10I	RCCGGY	1 cut(s)	1376
Cfr13I	GGNCC	8 cut(s)	38, 792, 1284, 1318, 2331, 2349, 2426, 2542
Cfr9I	CCCGGG	1 cut(s)	136
CseI	GACGC	1 cut(s)	2942
CsiI	ACCWGGT	1 cut(s)	1194
DraI	TTTAAA	1 cut(s)	2701
DraIII	CACNNNGTG	1 cut(s)	2762
EaeI	YGGCCR	3 cut(s)	1076, 1310, 3016
Eam1104I	CTCTTC	3 cut(s)	420, 1631, 2005
EarI	CTCTTC	3 cut(s)	420, 1631, 2005
EciI	GGCGGA	1 cut(s)	1801
Ecl136II	GAGCTC	2 cut(s)	9, 2923
Eco130I	CCWWGG	4 cut(s)	197, 223, 618, 2335
Eco24I	GRGCYC	4 cut(s)	11, 1930, 2227, 2925
Eco32I	GATATC	1 cut(s)	2980
Eco47I	GGWCC	7 cut(s)	792, 1284, 1318, 2331, 2349, 2426, 2542
Eco47III	AGCGCT	2 cut(s)	640, 1889
Eco53kI	GAGCTC	2 cut(s)	9, 2923
Eco57I	CTGAAG	5 cut(s)	536, 546, 1531, 2293, 3464
Eco72I	CACGTG	1 cut(s)	3248
Eco81I	CCTNAGG	1 cut(s)	3535
Eco88I	CYCGRG	1 cut(s)	136
EcoICRI	GAGCTC	2 cut(s)	9, 2923
EcoO109I	RGGNCCY	1 cut(s)	2542
EcoRI	GAATTC	1 cut(s)	2295
EcoRII	CCWGG	4 cut(s)	950, 1194, 2235, 3489
EcoRV	GATATC	1 cut(s)	2980
EcoT14I	CCWWGG	4 cut(s)	197, 223, 618, 2335
EcoT38I	GRGCYC	4 cut(s)	11, 1930, 2227, 2925
ErhI	CCWWGG	4 cut(s)	197, 223, 618, 2335
Esp3I	CGTCTC	1 cut(s)	48
FaqI	GGGAC	2 cut(s)	153, 3164
FauNDI	CATATG	2 cut(s)	1012, 3616
FbaI	TGATCA	1 cut(s)	3009
FblI	GTMKAC	1 cut(s)	2832
Fnu4HI	GCNGC	7 cut(s)	217, 637, 1374, 1845, 2573, 2885, 3036
FriOI	GRGCYC	4 cut(s)	11, 1930, 2227, 2925
Fsp4HI	GCNGC	7 cut(s)	217, 637, 1374, 1845, 2573, 2885, 3036
FspBI	CTAG	9 cut(s)	198, 1304, 1725, 1776, 1883, 2634, 2918, 3435, 3578
GlaI	GCGC	5 cut(s)	640, 998, 1818, 1889, 3206
GluI	GCNGC	7 cut(s)	217, 637, 1374, 1845, 2573, 2885, 3036
GsuI	CTGGAG	3 cut(s)	1146, 1918, 3050
HaeII	RGCGCY	2 cut(s)	642, 1891
HaeIII	GGCC	7 cut(s)	40, 1078, 1120, 1312, 2617, 2778, 3018
HapII	CCGG	5 cut(s)	34, 137, 422, 1117, 1377
HgaI	GACGC	1 cut(s)	2942
HhaI	GCGC	5 cut(s)	641, 999, 1819, 1890, 3207
Hin6I	GCGC	5 cut(s)	639, 997, 1817, 1888, 3205
HinP1I	GCGC	5 cut(s)	639, 997, 1817, 1888, 3205
HincII	GTYRAC	4 cut(s)	559, 719, 2833, 3396
HindII	GTYRAC	4 cut(s)	559, 719, 2833, 3396
HindIII	AAGCTT	2 cut(s)	1022, 3089
HpaI	GTTAAC	1 cut(s)	3396
HpaII	CCGG	5 cut(s)	34, 137, 422, 1117, 1377
HphI	GGTGA	3 cut(s)	1030, 2333, 3202
Hpy166II	GTNNAC	8 cut(s)	411, 559, 662, 719, 2833, 3396, 3587, 3612
Hpy8I	GTNNAC	8 cut(s)	411, 559, 662, 719, 2833, 3396, 3587, 3612
HpyCH4IV	ACGT	2 cut(s)	861, 3247
HpyF10VI	GCNNNNNNNGC	9 cut(s)	645, 1117, 1309, 1481, 1825, 1885, 2222, 3565, 3569
HpySE526I	ACGT	2 cut(s)	861, 3247
HspAI	GCGC	5 cut(s)	639, 997, 1817, 1888, 3205
KpnI	GGTACC	1 cut(s)	488
Ksp22I	TGATCA	1 cut(s)	3009
KspAI	GTTAAC	1 cut(s)	3396
LguI	GCTCTTC	1 cut(s)	1631
LmnI	GCTCC	8 cut(s)	14, 547, 646, 700, 2230, 2303, 3073, 3256
Lsp1109I	GCAGC	6 cut(s)	648, 1360, 1831, 2559, 2871, 3047
LweI	GCATC	7 cut(s)	1525, 1572, 2136, 2242, 2401, 3184, 3216
MabI	ACCWGGT	1 cut(s)	1194
MaeI	CTAG	9 cut(s)	198, 1304, 1725, 1776, 1883, 2634, 2918, 3435, 3578
MaeII	ACGT	2 cut(s)	861, 3247
MaeIII	GTNAC	6 cut(s)	319, 1180, 1906, 2188, 2397, 3263
MfeI	CAATTG	5 cut(s)	150, 917, 976, 2229, 3658
MflI	RGATCY	3 cut(s)	250, 2566, 3633
MlsI	TGGCCA	2 cut(s)	1078, 1312
MluNI	TGGCCA	2 cut(s)	1078, 1312
MlyI	GAGTC	6 cut(s)	101, 119, 1747, 2369, 3173, 3283
MmeI	TCCRAC	6 cut(s)	96, 278, 1311, 1613, 2886, 3098
Mox20I	TGGCCA	2 cut(s)	1078, 1312
MroXI	GAANNNNTTC	3 cut(s)	29, 1164, 2516
MscI	TGGCCA	2 cut(s)	1078, 1312
MslI	CAYNNNNRTG	5 cut(s)	128, 479, 1347, 2319, 3239
Msp20I	TGGCCA	2 cut(s)	1078, 1312
MspA1I	CMGCKG	2 cut(s)	15, 577
MspCI	CTTAAG	1 cut(s)	3092
MspI	CCGG	5 cut(s)	34, 137, 422, 1117, 1377
MspR9I	CCNGG	7 cut(s)	35, 137, 138, 952, 1196, 2237, 3491
MunI	CAATTG	5 cut(s)	150, 917, 976, 2229, 3658
Mva1269I	GAATGC	3 cut(s)	78, 1054, 2750
MvaI	CCWGG	4 cut(s)	952, 1196, 2237, 3491
MwoI	GCNNNNNNNGC	9 cut(s)	645, 1117, 1309, 1481, 1825, 1885, 2222, 3565, 3569
NciI	CCSGG	3 cut(s)	35, 137, 138
NcoI	CCATGG	1 cut(s)	223
NdeI	CATATG	2 cut(s)	1012, 3616
NlaIV	GGNNCC	9 cut(s)	39, 252, 486, 1320, 1430, 1880, 2226, 2305, 2333
NmuCI	GTSAC	4 cut(s)	319, 1906, 2397, 3263
NspI	RCATGY	3 cut(s)	1942, 3244, 3271
NspV	TTCGAA	1 cut(s)	1417
PagI	TCATGA	3 cut(s)	474, 2020, 3234
PciSI	GCTCTTC	1 cut(s)	1631
PcsI	WCGNNNNNNNCGW	2 cut(s)	163, 274
PctI	GAATGC	3 cut(s)	78, 1054, 2750
PdmI	GAANNNNTTC	3 cut(s)	29, 1164, 2516
PfeI	GAWTC	7 cut(s)	702, 1160, 1354, 1789, 2641, 3052, 3354
PflMI	CCANNNNNTGG	1 cut(s)	1074
PfoI	TCCNGGA	1 cut(s)	3489
PkrI	GCNGC	7 cut(s)	218, 638, 1375, 1846, 2574, 2886, 3037
PleI	GAGTC	6 cut(s)	100, 118, 1746, 2368, 3172, 3283
PmaCI	CACGTG	1 cut(s)	3248
PmlI	CACGTG	1 cut(s)	3248
PpsI	GAGTC	6 cut(s)	100, 118, 1746, 2368, 3172, 3283
Ppu21I	YACGTR	1 cut(s)	3248
PpuMI	RGGWCCY	1 cut(s)	2542
PsiI	TTATAA	1 cut(s)	1803
Psp124BI	GAGCTC	2 cut(s)	11, 2925
Psp5II	RGGWCCY	1 cut(s)	2542
Psp6I	CCWGG	4 cut(s)	950, 1194, 2235, 3489
PspCI	CACGTG	1 cut(s)	3248
PspGI	CCWGG	4 cut(s)	950, 1194, 2235, 3489
PspN4I	GGNNCC	9 cut(s)	39, 252, 486, 1320, 1430, 1880, 2226, 2305, 2333
PspPI	GGNCC	8 cut(s)	38, 792, 1284, 1318, 2331, 2349, 2426, 2542
PspPPI	RGGWCCY	1 cut(s)	2542
PsrI	GAACNNNNNNTAC	2 cut(s)	1498, 1530
PstI	CTGCAG	1 cut(s)	3378
PstNI	CAGNNNCTG	1 cut(s)	514
PsuI	RGATCY	3 cut(s)	250, 2566, 3633
RseI	CAYNNNNRTG	5 cut(s)	128, 479, 1347, 2319, 3239
SacI	GAGCTC	2 cut(s)	11, 2925
SalI	GTCGAC	1 cut(s)	2831
SapI	GCTCTTC	1 cut(s)	1631
SatI	GCNGC	7 cut(s)	217, 637, 1374, 1845, 2573, 2885, 3036
Sau96I	GGNCC	8 cut(s)	38, 792, 1284, 1318, 2331, 2349, 2426, 2542
ScaI	AGTACT	2 cut(s)	459, 1507
SchI	GAGTC	6 cut(s)	101, 119, 1747, 2369, 3173, 3283
ScrFI	CCNGG	7 cut(s)	35, 137, 138, 952, 1196, 2237, 3491
SexAI	ACCWGGT	1 cut(s)	1194
SfaNI	GCATC	7 cut(s)	1525, 1572, 2136, 2242, 2401, 3184, 3216
SfcI	CTRYAG	6 cut(s)	728, 1435, 1683, 1743, 3374, 3460
SfuI	TTCGAA	1 cut(s)	1417
SinI	GGWCC	7 cut(s)	792, 1284, 1318, 2331, 2349, 2426, 2542
SmaI	CCCGGG	1 cut(s)	138
SmiMI	CAYNNNNRTG	5 cut(s)	128, 479, 1347, 2319, 3239
SmlI	CTYRAG	1 cut(s)	3092
SmoI	CTYRAG	1 cut(s)	3092
SsiI	CCGC	7 cut(s)	15, 173, 217, 575, 1538, 1786, 2872
SspI	AATATT	3 cut(s)	1032, 2200, 2274
SspMI	CTAG	9 cut(s)	198, 1304, 1725, 1776, 1883, 2634, 2918, 3435, 3578
SstI	GAGCTC	2 cut(s)	11, 2925
StyD4I	CCNGG	7 cut(s)	33, 135, 136, 950, 1194, 2235, 3489
StyI	CCWWGG	4 cut(s)	197, 223, 618, 2335
TaiI	ACGT	2 cut(s)	864, 3250
TaqI	TCGA	7 cut(s)	56, 166, 277, 761, 1417, 1450, 2832
TaqII	GACCGA	1 cut(s)	36
TatI	WGTACW	8 cut(s)	408, 457, 625, 910, 1203, 1505, 2727, 3456
TauI	GCSGC	1 cut(s)	219
TfiI	GAWTC	7 cut(s)	702, 1160, 1354, 1789, 2641, 3052, 3354
TseFI	GTSAC	4 cut(s)	319, 1906, 2397, 3263
TseI	GCWGC	6 cut(s)	636, 1373, 1844, 2572, 2884, 3035
Tsp45I	GTSAC	4 cut(s)	319, 1906, 2397, 3263
TspGWI	ACGGA	3 cut(s)	633, 1888, 2344
TspMI	CCCGGG	1 cut(s)	136
Van91I	CCANNNNNTGG	1 cut(s)	1074
Vha464I	CTTAAG	1 cut(s)	3092
VneI	GTGCAC	1 cut(s)	3585
VpaK11BI	GGWCC	7 cut(s)	792, 1284, 1318, 2331, 2349, 2426, 2542
XbaI	TCTAGA	3 cut(s)	2917, 3434, 3577
XceI	RCATGY	3 cut(s)	1942, 3244, 3271
XcmI	CCANNNNNNNNNTGG	2 cut(s)	85, 802
XmaI	CCCGGG	1 cut(s)	136
XmaJI	CCTAGG	1 cut(s)	197
XmiI	GTMKAC	1 cut(s)	2832
XmnI	GAANNNNTTC	3 cut(s)	29, 1164, 2516
XspI	CTAG	9 cut(s)	198, 1304, 1725, 1776, 1883, 2634, 2918, 3435, 3578
ZrmI	AGTACT	2 cut(s)	459, 1507

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.