AT3G16100

RAB GTPase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0020840
Physical Location & Seq
Forward (+)
5459162 .. 5460816
1655 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G16100.1

Sequence Viewer

Length: 621 bp
ATGGCTTCTCGGCGGCGAGTGCTTCTGAAGGTTATAATTCTCGGAGACAGTGGGGTTGGGAAGACGTCCTTGATGAATCAGTTTGTGAATCGAAAATTCAGTAATCAGTATAAAGCTACCATTGGAGCTGATTTCTTGACCAAAGAGGTCCAAATTGATGACAGAATCTTCACTTTGCAGATTTGGGATACTGCAGGGCAAGAGAGGTTCCAAAGTTTGGGTGTTGCTTTCTACCGAGGAGCGGACTGTTGTGTTCTTGTGAATGATGTCAATGTTATGAAATCCTTTGAAAATCTTAACAACTGGAGGGAAGAGTTCCTGATCCAGGCTAGTCCTTCAGATCCTGAAAACTTCCCCTTCGTTGTCTTGGGGAACAAGACAGATGTTGACGGTGGAAAGAGTCGAGTGGTTACTGAGAAGAAAGCAAAATCTTGGTGTGCATCCAAGGGAAACATTCCTTACTTCGAGACTTCGGCCAAAGATGGAGTCAATGTTGATGCAGCTTTCGAATGCATTGCCAAAAATGCTCTCAAGAATGAACCTGAAGAAGAAGTGTACCTTCCGGACACCATTGATGTAGCTGGGGCTCGGCAACAAAGATCAACTGGGTGCGAATGCTAA

Protein Analysis

206

Amino Acids

22.97

Weight (kDa)

5.4

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 9 - 174 2.1e-12 ADP-ribosylation factor family
Ras PF00071 10 - 176 8.4e-54 Ras family
Roc PF08477 10 - 128 6.4e-32 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 10 - 103 3.4e-07 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020840)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52280 AT3G16100
rosa_chinensis RchiOBHm_Chr1g0315361
rosa_wichuraiana Rw1G014070 Rw1G023270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 35
AatII GACGTC 1 cut(s) 68
AccB7I CCANNNNNTGG 1 cut(s) 217
AccBSI CCGCTC 1 cut(s) 242
AccIII TCCGGA 1 cut(s) 562
AciI CCGC 2 cut(s) 13, 242
AclWI GGATC 2 cut(s) 316, 335
AcoI YGGCCR 1 cut(s) 474
AcsI RAATTY 1 cut(s) 95
AcuI CTGAAG 3 cut(s) 47, 321, 564
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 1 cut(s) 557
AfiI CCNNNNNNNGG 3 cut(s) 217, 241, 325
AgsI TTSAA 1 cut(s) 290
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 4 cut(s) 116, 128, 503, 581
AluI AGCT 4 cut(s) 116, 128, 503, 581
Alw26I GTCTC 2 cut(s) 39, 461
AlwI GGATC 2 cut(s) 316, 335
AlwNI CAGNNNCTG 1 cut(s) 344
Aor13HI TCCGGA 1 cut(s) 562
AoxI GGCC 1 cut(s) 474
ApeKI GCWGC 1 cut(s) 500
ApoI RAATTY 1 cut(s) 95
ArsI GACNNNNNNTTYG 2 cut(s) 471, 503
AspS9I GGNCC 1 cut(s) 148
AsuII TTCGAA 1 cut(s) 507
AvaII GGWCC 1 cut(s) 148
BanII GRGCYC 1 cut(s) 589
BbsI GAAGAC 1 cut(s) 68
BbvI GCAGC 1 cut(s) 512
BccI CCATC 1 cut(s) 476
BcgI CGANNNNNNTGC 2 cut(s) 497, 531
BciT130I CCWGG 1 cut(s) 326
BciVI GTATCC 1 cut(s) 181
BcoDI GTCTC 2 cut(s) 39, 461
BfaI CTAG 1 cut(s) 330
BfmI CTRYAG 1 cut(s) 192
BfuI GTATCC 1 cut(s) 181
BisI GCNGC 2 cut(s) 14, 501
BlsI GCNGC 2 cut(s) 15, 502
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 1 cut(s) 209
BmrFI CCNGG 1 cut(s) 326
BmrI ACTGGG 1 cut(s) 615
BmsI GCATC 2 cut(s) 449, 487
BmuI ACTGGG 1 cut(s) 615
BpiI GAAGAC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 325
Bpu14I TTCGAA 1 cut(s) 507
BpuEI CTTGAG 1 cut(s) 515
BsaHI GRCGYC 1 cut(s) 65
BsaJI CCNNGG 2 cut(s) 235, 444
BsaWI WCCGGW 1 cut(s) 562
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 241, 325
Bse1I ACTGG 2 cut(s) 308, 610
Bse3DI GCAATG 1 cut(s) 513
BseAI TCCGGA 1 cut(s) 562
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 2 cut(s) 235, 444
BseGI GGATG 1 cut(s) 440
BseLI CCNNNNNNNGG 3 cut(s) 217, 241, 325
BseMI GCAATG 1 cut(s) 513
BseMII CTCAG 1 cut(s) 405
BseNI ACTGG 2 cut(s) 308, 610
BseRI GAGGAG 1 cut(s) 252
BseXI GCAGC 1 cut(s) 512
BseYI CCCAGC 1 cut(s) 581
BshFI GGCC 1 cut(s) 476
BsiSI CCGG 1 cut(s) 563
BslI CCNNNNNNNGG 3 cut(s) 217, 241, 325
BsmAI GTCTC 2 cut(s) 39, 461
BsmI GAATGC 2 cut(s) 515, 620
BsnI GGCC 1 cut(s) 476
Bsp119I TTCGAA 1 cut(s) 507
Bsp1286I GDGCHC 1 cut(s) 589
Bsp13I TCCGGA 1 cut(s) 562
Bsp143I GATC 3 cut(s) 321, 340, 599
BspACI CCGC 2 cut(s) 13, 242
BspANI GGCC 1 cut(s) 476
BspCNI CTCAG 1 cut(s) 406
BspEI TCCGGA 1 cut(s) 562
BspLI GGNNCC 1 cut(s) 209
BspMAI CTGCAG 1 cut(s) 196
BspPI GGATC 2 cut(s) 316, 335
BspT104I TTCGAA 1 cut(s) 507
BsrBI CCGCTC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 513
BsrI ACTGG 2 cut(s) 308, 610
BssECI CCNNGG 2 cut(s) 235, 444
BssMI GATC 3 cut(s) 321, 340, 599
BssNI GRCGYC 1 cut(s) 65
BssT1I CCWWGG 1 cut(s) 444
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 3 cut(s) 50, 248, 392
Bst6I CTCTTC 1 cut(s) 306
BstACI GRCGYC 1 cut(s) 65
BstBI TTCGAA 1 cut(s) 507
BstDEI CTNAG 1 cut(s) 414
BstENI CCTNNNNNAGG 1 cut(s) 323
BstF5I GGATG 1 cut(s) 440
BstKTI GATC 3 cut(s) 324, 343, 602
BstMAI GTCTC 2 cut(s) 39, 461
BstMBI GATC 3 cut(s) 321, 340, 599
BstMWI GCNNNNNNNGC 2 cut(s) 19, 524
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 1 cut(s) 324
BstSFI CTRYAG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 512
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 1 cut(s) 476
BtsCI GGATG 1 cut(s) 440
BtsIMutI CAGTG 1 cut(s) 55
CaiI CAGNNNCTG 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 148
Csp6I GTAC 1 cut(s) 556
CviJI RGCY 8 cut(s) 5, 116, 128, 329, 476, 503, 581, 587
CviKI_1 RGCY 8 cut(s) 5, 116, 128, 329, 476, 503, 581, 587
CviQI GTAC 1 cut(s) 556
DdeI CTNAG 1 cut(s) 414
DpnI GATC 3 cut(s) 323, 342, 601
DpnII GATC 3 cut(s) 321, 340, 599
EaeI YGGCCR 1 cut(s) 474
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 589
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 3 cut(s) 47, 321, 564
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 324
EcoT14I CCWWGG 1 cut(s) 444
EcoT22I ATGCAT 1 cut(s) 515
EcoT38I GRGCYC 1 cut(s) 589
ErhI CCWWGG 1 cut(s) 444
FaiI YATR 3 cut(s) 35, 111, 278
FalI AAGNNNNNCTT 4 cut(s) 53, 85, 543, 575
Fnu4HI GCNGC 2 cut(s) 14, 501
FokI GGATG 1 cut(s) 427
FriOI GRGCYC 1 cut(s) 589
Fsp4HI GCNGC 2 cut(s) 14, 501
FspBI CTAG 1 cut(s) 330
GluI GCNGC 2 cut(s) 14, 501
GsaI CCCAGC 1 cut(s) 585
GsuI CTGGAG 1 cut(s) 325
HaeIII GGCC 1 cut(s) 476
HapII CCGG 1 cut(s) 563
Hin1I GRCGYC 1 cut(s) 65
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 5 cut(s) 76, 88, 165, 400, 486
HpaII CCGG 1 cut(s) 563
Hpy166II GTNNAC 2 cut(s) 388, 556
Hpy188I TCNGA 3 cut(s) 27, 44, 340
Hpy188III TCNNGA 6 cut(s) 136, 319, 344, 466, 532, 563
Hpy8I GTNNAC 2 cut(s) 388, 556
HpyAV CCTTC 4 cut(s) 22, 345, 367, 569
HpyCH4III ACNGT 3 cut(s) 50, 248, 392
HpyCH4IV ACGT 1 cut(s) 65
HpyCH4V TGCA 5 cut(s) 178, 194, 440, 500, 513
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 524
HpyF3I CTNAG 1 cut(s) 414
HpySE526I ACGT 1 cut(s) 65
Hsp92I GRCGYC 1 cut(s) 65
Kpn2I TCCGGA 1 cut(s) 562
Kzo9I GATC 3 cut(s) 321, 340, 599
LmnI GCTCC 2 cut(s) 125, 239
Lsp1109I GCAGC 1 cut(s) 512
LweI GCATC 2 cut(s) 449, 487
MaeI CTAG 1 cut(s) 330
MaeII ACGT 1 cut(s) 65
MaeIII GTNAC 1 cut(s) 409
MalI GATC 3 cut(s) 323, 342, 601
MbiI CCGCTC 1 cut(s) 242
MboI GATC 3 cut(s) 321, 340, 599
MboII GAAGA 6 cut(s) 73, 160, 323, 430, 557, 560
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 1 cut(s) 589
MluCI AATT 3 cut(s) 36, 95, 153
MlyI GAGTC 2 cut(s) 409, 495
MnlI CCTC 4 cut(s) 139, 198, 230, 300
Mph1103I ATGCAT 1 cut(s) 515
MroI TCCGGA 1 cut(s) 562
MseI TTAA 1 cut(s) 297
MspI CCGG 1 cut(s) 563
MspR9I CCNGG 1 cut(s) 326
Mva1269I GAATGC 2 cut(s) 515, 620
MvaI CCWGG 1 cut(s) 326
MwoI GCNNNNNNNGC 2 cut(s) 19, 524
NdeII GATC 3 cut(s) 321, 340, 599
NlaIV GGNNCC 1 cut(s) 209
NmeAIII GCCGAG 1 cut(s) 568
NsiI ATGCAT 1 cut(s) 515
NspV TTCGAA 1 cut(s) 507
PctI GAATGC 2 cut(s) 515, 620
PfeI GAWTC 3 cut(s) 76, 88, 165
PflMI CCANNNNNTGG 1 cut(s) 217
PkrI GCNGC 2 cut(s) 15, 502
PleI GAGTC 2 cut(s) 408, 494
PpsI GAGTC 2 cut(s) 408, 494
PsiI TTATAA 1 cut(s) 35
Psp6I CCWGG 1 cut(s) 324
PspFI CCCAGC 1 cut(s) 581
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 1 cut(s) 209
PspPI GGNCC 1 cut(s) 148
PstI CTGCAG 1 cut(s) 196
PstNI CAGNNNCTG 1 cut(s) 344
PsuI RGATCY 1 cut(s) 340
RsaI GTAC 1 cut(s) 557
RsaNI GTAC 1 cut(s) 556
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 2 cut(s) 14, 501
Sau3AI GATC 3 cut(s) 321, 340, 599
Sau96I GGNCC 1 cut(s) 148
SchI GAGTC 2 cut(s) 409, 495
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 1 cut(s) 589
SfaNI GCATC 2 cut(s) 449, 487
SfcI CTRYAG 1 cut(s) 192
SfuI TTCGAA 1 cut(s) 507
SinI GGWCC 1 cut(s) 148
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
Sse9I AATT 3 cut(s) 36, 95, 153
SsiI CCGC 2 cut(s) 13, 242
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 1 cut(s) 444
TaaI ACNGT 3 cut(s) 50, 248, 392
TaiI ACGT 1 cut(s) 68
TaqI TCGA 4 cut(s) 91, 403, 465, 507
TasI AATT 3 cut(s) 36, 95, 153
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 3 cut(s) 76, 88, 165
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TscAI CASTG 1 cut(s) 55
TseI GCWGC 1 cut(s) 500
TspDTI ATGAA 3 cut(s) 89, 293, 552
TspRI CASTG 1 cut(s) 55
Van91I CCANNNNNTGG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 148
XagI CCTNNNNNAGG 1 cut(s) 323
XapI RAATTY 1 cut(s) 95
XspI CTAG 1 cut(s) 330
ZraI GACGTC 1 cut(s) 66
Zsp2I ATGCAT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.