Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0019602

Physical Location & Seq

Genomic Coordinates Forward (+)

23172754 .. 23174993

Gene Structure 2240 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G62660.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGCTTTGGATCATGAGATTCTCCGGTTTATTCTCCGCCGCTTTGGTTATCATCGTCCTCTCTCCTTCTCTCCAATCGTTTCCTCCAGCTGAAGCTATCAGATCCTCTCATCTCGACGCTTACCTCCGTTTCCCCTCCTCCGATCCACCGCCGCATAGATTCTCCTTCAGAAAAGCTCCTGTTTTCCGCAATGCCGCCGATTGCGCCGCCGCAGATATCGATTCCGGCGTCTGTAACCCTTCCTTGGTCCACGTCGCGATTACTCTCGATTTCGAGTACCTGCGTGGCTCAATCGCCGCCGTTCATTCGATTCTCAAGCACTCGTCGTGTCCCGAGAGCGTCTTCTTCCATTTCCTCGTCTCCGAGACTGACCTAGAATCCTTGATTCGTTCGACTTTTCCCGAATTGAAATTAAAGGTTTACTACTTCGATCCGGAGATTGTACGGACGCTGATCTCAACCTCCGTGAGACAAGCGCTCGAGCAGCCGTTGAATTACGCTAGAAATTACCTAGCTGACCTTCTCGAGCCTTGCGTGCGTCGCGTGATCTACCTAGATTCCGATCTAATCGTCGTCGACGACATCGCAAAGCTCTGGATGACGAAACTGGGATCGAAAACGATCGGAGCTCCCGAGTACTGTCACGCGAACTTCACAAAGTATTTCACACCGGCGTTCTGGTCCGACGAGAGGTTCTCCGGAGCTTTCTCCGGGAGGAAACCGTGCTACTTCAACACGGGAGTGATGGTGATGGATCTAGAGAGATGGAGGCGCGTAGGGTACACGGAGGTGATAGAGAAATGGATGGAGATTCAGAAGAGTGATAGGATTTACGAGCTGGGATCATTGCCGCCGTTCTTGTTGGTGTTCGCCGGAGAAGTAGCTCCGATAGAGCATCGGTGGAACCAGCATGGGCTTGGTGGAGATAACGTGAGAGGAAGCTGTAGAGATTTACATCCCGGTCCGGTTAGCTTGCTTCATTGGTCCGGTAGTGGTAAACCGTGGTTTCGGTTAGATTCGAGACGGCCTTGTCCACTTGATACTCTTTGGGCACCTTATGATTTGTATGGACACTACTCTCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000271 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005975 GO:0005976 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009059 GO:0009664 GO:0009791 GO:0009832 GO:0009987 GO:0010154 GO:0010191 GO:0010192 GO:0010214 GO:0010393 GO:0012505 GO:0016043 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0017144 GO:0022414 GO:0022607 GO:0032501 GO:0032502 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0045488 GO:0047262 GO:0048316 GO:0048354 GO:0048359 GO:0048363 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0070726 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071704 GO:0071840 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_transf_8

Protein Analysis

361

Amino Acids

41.12

Weight (kDa)

6.86

Isoelectric Point (pI)

51.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_8	PF01501	84 - 337	1.1e-61	Glycosyl transferase family 8
Glyco_transf_24	PF18404	122 - 265	3e-06	Glucosyltransferase 24

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0019602)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G62660 AT4G02130 AT4G02130 AT4G02130
prunus_persica	Prupe.8G268500_v2.0.a1 Prupe.8G268500_v2.0.a1
pyrus_communis	pycom11g27730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1029
Acc36I	ACCTGC	1 cut(s)	288
AccB1I	GGYRCC	1 cut(s)	1051
AccI	GTMKAC	1 cut(s)	576
AccII	CGCG	4 cut(s)	257, 543, 647, 774
AccIII	TCCGGA	2 cut(s)	433, 698
AclWI	GGATC	7 cut(s)	17, 96, 137, 425, 619, 762, 850
AcuI	CTGAAG	2 cut(s)	111, 151
AcyI	GRCGYC	1 cut(s)	228
AfaI	GTAC	4 cut(s)	278, 444, 638, 782
AfeI	AGCGCT	1 cut(s)	477
AfiI	CCNNNNNNNGG	2 cut(s)	244, 690
AgsI	TTSAA	3 cut(s)	409, 493, 733
AjiI	CACGTC	1 cut(s)	253
AleI	CACNNNNGTG	2 cut(s)	740, 788
Alw21I	GWGCWC	1 cut(s)	631
Alw26I	GTCTC	4 cut(s)	359, 364, 463, 1015
AlwI	GGATC	7 cut(s)	17, 96, 137, 425, 619, 762, 850
Ama87I	CYCGRG	4 cut(s)	332, 479, 524, 632
Aor13HI	TCCGGA	2 cut(s)	433, 698
Aor51HI	AGCGCT	1 cut(s)	477
AoxI	GGCC	1 cut(s)	1025
ApeKI	GCWGC	1 cut(s)	484
AspLEI	GCGC	3 cut(s)	206, 478, 774
AspS9I	GGNCC	4 cut(s)	247, 681, 962, 984
AsuC2I	CCSGG	2 cut(s)	712, 960
AsuHPI	GGTGA	2 cut(s)	760, 802
AvaI	CYCGRG	4 cut(s)	332, 479, 524, 632
AvaII	GGWCC	4 cut(s)	247, 681, 962, 984
BaeGI	GKGCMC	1 cut(s)	1054
BanI	GGYRCC	1 cut(s)	1051
BanII	GRGCYC	1 cut(s)	631
BbsI	GAAGAC	1 cut(s)	334
Bbv12I	GWGCWC	1 cut(s)	631
BbvI	GCAGC	1 cut(s)	496
BccI	CCATC	4 cut(s)	739, 745, 759, 799
BceAI	ACGGC	4 cut(s)	284, 472, 838, 1040
BcnI	CCSGG	2 cut(s)	712, 960
BcoDI	GTCTC	4 cut(s)	359, 364, 463, 1015
BfaI	CTAG	5 cut(s)	374, 501, 512, 554, 758
BfmI	CTRYAG	1 cut(s)	943
BfoI	RGCGCY	1 cut(s)	479
BfuAI	ACCTGC	1 cut(s)	288
BisI	GCNGC	8 cut(s)	39, 152, 195, 207, 210, 297, 485, 851
BlsI	GCNGC	8 cut(s)	40, 153, 196, 208, 211, 298, 486, 852
BmcAI	AGTACT	1 cut(s)	638
Bme1390I	CCNGG	2 cut(s)	712, 960
Bme18I	GGWCC	4 cut(s)	247, 681, 962, 984
BmeT110I	CYCGRG	4 cut(s)	332, 479, 524, 632
BmgBI	CACGTC	1 cut(s)	253
BmgT120I	GGNCC	4 cut(s)	247, 681, 962, 984
BmiI	GGNNCC	2 cut(s)	905, 1053
BmrFI	CCNGG	2 cut(s)	712, 960
BmrI	ACTGGG	1 cut(s)	617
BmsI	GCATC	1 cut(s)	904
BmuI	ACTGGG	1 cut(s)	617
BpiI	GAAGAC	1 cut(s)	334
BplI	GAGNNNNNCTC	2 cut(s)	680, 712
BpmI	CTGGAG	1 cut(s)	69
BpuEI	CTTGAG	1 cut(s)	299
BpuMI	CCSGG	2 cut(s)	712, 960
Bsa29I	ATCGAT	1 cut(s)	219
BsaBI	GATNNNNATC	2 cut(s)	561, 954
BsaHI	GRCGYC	1 cut(s)	228
BsaJI	CCNNGG	2 cut(s)	243, 1001
BsaWI	WCCGGW	5 cut(s)	23, 433, 698, 964, 986
BsaXI	ACNNNNNCTCC	4 cut(s)	732, 762, 915, 945
Bsc4I	CCNNNNNNNGG	2 cut(s)	244, 690
Bse118I	RCCGGY	1 cut(s)	670
Bse1I	ACTGG	1 cut(s)	612
Bse3DI	GCAATG	2 cut(s)	196, 845
Bse8I	GATNNNNATC	2 cut(s)	561, 954
BseAI	TCCGGA	2 cut(s)	433, 698
BseCI	ATCGAT	1 cut(s)	219
BseDI	CCNNGG	2 cut(s)	243, 1001
BseGI	GGATG	3 cut(s)	603, 810, 955
BseJI	GATNNNNATC	2 cut(s)	561, 954
BseLI	CCNNNNNNNGG	2 cut(s)	244, 690
BseMI	GCAATG	2 cut(s)	196, 845
BseNI	ACTGG	1 cut(s)	612
BseRI	GAGGAG	1 cut(s)	127
BseSI	GKGCMC	1 cut(s)	1054
BseXI	GCAGC	1 cut(s)	496
BseYI	CCCAGC	1 cut(s)	838
Bsh1236I	CGCG	4 cut(s)	257, 543, 647, 774
Bsh1285I	CGRYCG	1 cut(s)	624
BshFI	GGCC	1 cut(s)	1027
BshNI	GGYRCC	1 cut(s)	1051
BshVI	ATCGAT	1 cut(s)	219
BsiEI	CGRYCG	1 cut(s)	624
BsiHKAI	GWGCWC	1 cut(s)	631
BsiHKCI	CYCGRG	4 cut(s)	332, 479, 524, 632
BslFI	GGGAC	1 cut(s)	315
BslI	CCNNNNNNNGG	2 cut(s)	244, 690
BsmAI	GTCTC	4 cut(s)	359, 364, 463, 1015
BsmBI	CGTCTC	2 cut(s)	364, 1015
BsmFI	GGGAC	1 cut(s)	315
BsnI	GGCC	1 cut(s)	1027
BsoBI	CYCGRG	4 cut(s)	332, 479, 524, 632
Bsp1286I	GDGCHC	2 cut(s)	631, 1054
Bsp13I	TCCGGA	2 cut(s)	433, 698
Bsp68I	TCGCGA	1 cut(s)	257
BspANI	GGCC	1 cut(s)	1027
BspDI	ATCGAT	1 cut(s)	219
BspEI	TCCGGA	2 cut(s)	433, 698
BspFNI	CGCG	4 cut(s)	257, 543, 647, 774
BspHI	TCATGA	1 cut(s)	12
BspLI	GGNNCC	2 cut(s)	905, 1053
BspMI	ACCTGC	1 cut(s)	288
BspPI	GGATC	7 cut(s)	17, 96, 137, 425, 619, 762, 850
BspT107I	GGYRCC	1 cut(s)	1051
BsrDI	GCAATG	2 cut(s)	196, 845
BsrFI	RCCGGY	1 cut(s)	670
BsrI	ACTGG	1 cut(s)	612
BssAI	RCCGGY	1 cut(s)	670
BssECI	CCNNGG	2 cut(s)	243, 1001
BssNI	GRCGYC	1 cut(s)	228
BssT1I	CCWWGG	1 cut(s)	243
Bst4CI	ACNGT	3 cut(s)	641, 723, 1002
Bst6I	CTCTTC	1 cut(s)	812
BstACI	GRCGYC	1 cut(s)	228
BstC8I	GCNNGC	2 cut(s)	536, 974
BstDSI	CCRYGG	1 cut(s)	1001
BstF5I	GGATG	3 cut(s)	603, 810, 955
BstFNI	CGCG	4 cut(s)	257, 543, 647, 774
BstH2I	RGCGCY	1 cut(s)	479
BstHHI	GCGC	3 cut(s)	206, 478, 774
BstMAI	GTCTC	4 cut(s)	359, 364, 463, 1015
BstMCI	CGRYCG	1 cut(s)	624
BstMWI	GCNNNNNNNGC	4 cut(s)	203, 484, 535, 540
BstSCI	CCNGG	2 cut(s)	710, 958
BstSFI	CTRYAG	1 cut(s)	943
BstSLI	GKGCMC	1 cut(s)	1054
BstUI	CGCG	4 cut(s)	257, 543, 647, 774
BstV1I	GCAGC	1 cut(s)	496
BstV2I	GAAGAC	1 cut(s)	334
BstX2I	RGATCY	2 cut(s)	101, 754
BstYI	RGATCY	2 cut(s)	101, 754
Bsu15I	ATCGAT	1 cut(s)	219
BsuRI	GGCC	1 cut(s)	1027
BsuTUI	ATCGAT	1 cut(s)	219
BtgI	CCRYGG	1 cut(s)	1001
BtgZI	GCGATG	1 cut(s)	568
BtrI	CACGTC	1 cut(s)	253
BtsCI	GGATG	3 cut(s)	603, 810, 955
BtuMI	TCGCGA	1 cut(s)	257
BveI	ACCTGC	1 cut(s)	288
Cac8I	GCNNGC	2 cut(s)	536, 974
CciI	TCATGA	1 cut(s)	12
CfoI	GCGC	3 cut(s)	206, 478, 774
Cfr10I	RCCGGY	1 cut(s)	670
Cfr13I	GGNCC	4 cut(s)	247, 681, 962, 984
ClaI	ATCGAT	1 cut(s)	219
CpoI	CGGWCCG	1 cut(s)	962
CseI	GACGC	5 cut(s)	125, 217, 328, 457, 527
Csp6I	GTAC	4 cut(s)	277, 443, 637, 781
CspI	CGGWCCG	1 cut(s)	962
CviAII	CATG	2 cut(s)	13, 911
CviQI	GTAC	4 cut(s)	277, 443, 637, 781
DrdI	GACNNNNNNGTC	1 cut(s)	1029
DseDI	GACNNNNNNGTC	1 cut(s)	1029
Eam1104I	CTCTTC	1 cut(s)	812
EarI	CTCTTC	1 cut(s)	812
EciI	GGCGGA	1 cut(s)	25
Ecl136II	GAGCTC	1 cut(s)	629
Eco130I	CCWWGG	1 cut(s)	243
Eco24I	GRGCYC	1 cut(s)	631
Eco32I	GATATC	1 cut(s)	217
Eco47I	GGWCC	4 cut(s)	247, 681, 962, 984
Eco47III	AGCGCT	1 cut(s)	477
Eco53kI	GAGCTC	1 cut(s)	629
Eco57I	CTGAAG	2 cut(s)	111, 151
Eco88I	CYCGRG	4 cut(s)	332, 479, 524, 632
EcoICRI	GAGCTC	1 cut(s)	629
EcoRV	GATATC	1 cut(s)	217
EcoT14I	CCWWGG	1 cut(s)	243
EcoT38I	GRGCYC	1 cut(s)	631
ErhI	CCWWGG	1 cut(s)	243
Esp3I	CGTCTC	2 cut(s)	364, 1015
FaeI	CATG	2 cut(s)	16, 914
FaiI	YATR	5 cut(s)	14, 156, 912, 1059, 1068
FaqI	GGGAC	1 cut(s)	315
FatI	CATG	2 cut(s)	12, 910
FblI	GTMKAC	1 cut(s)	576
Fnu4HI	GCNGC	8 cut(s)	39, 152, 195, 207, 210, 297, 485, 851
FokI	GGATG	3 cut(s)	610, 817, 942
FriOI	GRGCYC	1 cut(s)	631
Fsp4HI	GCNGC	8 cut(s)	39, 152, 195, 207, 210, 297, 485, 851
FspBI	CTAG	5 cut(s)	374, 501, 512, 554, 758
GlaI	GCGC	3 cut(s)	205, 477, 773
GluI	GCNGC	8 cut(s)	39, 152, 195, 207, 210, 297, 485, 851
GsaI	CCCAGC	1 cut(s)	842
GsuI	CTGGAG	1 cut(s)	69
HaeII	RGCGCY	1 cut(s)	479
HaeIII	GGCC	1 cut(s)	1027
HgaI	GACGC	5 cut(s)	125, 217, 328, 457, 527
HhaI	GCGC	3 cut(s)	206, 478, 774
Hin1I	GRCGYC	1 cut(s)	228
Hin1II	CATG	2 cut(s)	16, 914
Hin6I	GCGC	3 cut(s)	204, 476, 772
HinP1I	GCGC	3 cut(s)	204, 476, 772
HincII	GTYRAC	1 cut(s)	577
HindII	GTYRAC	1 cut(s)	577
HinfI	GANTC	9 cut(s)	18, 159, 221, 310, 377, 385, 557, 811, 1016
HphI	GGTGA	2 cut(s)	760, 802
Hpy166II	GTNNAC	6 cut(s)	250, 421, 577, 783, 998, 1034
Hpy188I	TCNGA	9 cut(s)	101, 142, 170, 364, 562, 626, 685, 816, 888
Hpy8I	GTNNAC	6 cut(s)	250, 421, 577, 783, 998, 1034
Hpy99I	CGWCG	8 cut(s)	119, 257, 328, 543, 575, 578, 581, 689
HpyAV	CCTTC	4 cut(s)	75, 175, 249, 530
HpyCH4III	ACNGT	3 cut(s)	641, 723, 1002
HpyCH4IV	ACGT	2 cut(s)	252, 930
HpyF10VI	GCNNNNNNNGC	4 cut(s)	203, 484, 535, 540
HpySE526I	ACGT	2 cut(s)	252, 930
Hsp92I	GRCGYC	1 cut(s)	228
Hsp92II	CATG	2 cut(s)	16, 914
HspAI	GCGC	3 cut(s)	204, 476, 772
Kpn2I	TCCGGA	2 cut(s)	433, 698
LmnI	GCTCC	5 cut(s)	181, 626, 634, 701, 889
Lsp1109I	GCAGC	1 cut(s)	496
LweI	GCATC	1 cut(s)	904
MaeI	CTAG	5 cut(s)	374, 501, 512, 554, 758
MaeII	ACGT	2 cut(s)	252, 930
MaeIII	GTNAC	2 cut(s)	233, 641
MboII	GAAGA	3 cut(s)	334, 337, 829
MflI	RGATCY	2 cut(s)	101, 754
MhlI	GDGCHC	2 cut(s)	631, 1054
MluCI	AATT	4 cut(s)	404, 410, 493, 505
MmeI	TCCRAC	1 cut(s)	708
MroI	TCCGGA	2 cut(s)	433, 698
MseI	TTAA	1 cut(s)	413
MslI	CAYNNNNRTG	2 cut(s)	740, 788
MspA1I	CMGCKG	1 cut(s)	89
MspR9I	CCNGG	2 cut(s)	712, 960
MvnI	CGCG	4 cut(s)	257, 543, 647, 774
MwoI	GCNNNNNNNGC	4 cut(s)	203, 484, 535, 540
NciI	CCSGG	2 cut(s)	712, 960
NlaIII	CATG	2 cut(s)	16, 914
NlaIV	GGNNCC	2 cut(s)	905, 1053
NmuCI	GTSAC	1 cut(s)	641
NruI	TCGCGA	1 cut(s)	257
OliI	CACNNNNGTG	2 cut(s)	740, 788
PaeR7I	CTCGAG	2 cut(s)	479, 524
PagI	TCATGA	1 cut(s)	12
PcsI	WCGNNNNNNNCGW	4 cut(s)	225, 531, 576, 630
PfeI	GAWTC	9 cut(s)	18, 159, 221, 310, 377, 385, 557, 811, 1016
PfoI	TCCNGGA	1 cut(s)	710
PkrI	GCNGC	8 cut(s)	40, 153, 196, 208, 211, 298, 486, 852
Ple19I	CGATCG	1 cut(s)	624
Psp124BI	GAGCTC	1 cut(s)	631
PspFI	CCCAGC	1 cut(s)	838
PspN4I	GGNNCC	2 cut(s)	905, 1053
PspPI	GGNCC	4 cut(s)	247, 681, 962, 984
PspXI	VCTCGAGB	1 cut(s)	479
PsuI	RGATCY	2 cut(s)	101, 754
PvuI	CGATCG	1 cut(s)	624
PvuII	CAGCTG	1 cut(s)	89
RruI	TCGCGA	1 cut(s)	257
RsaI	GTAC	4 cut(s)	278, 444, 638, 782
RsaNI	GTAC	4 cut(s)	277, 443, 637, 781
RseI	CAYNNNNRTG	2 cut(s)	740, 788
Rsr2I	CGGWCCG	1 cut(s)	962
RsrII	CGGWCCG	1 cut(s)	962
SacI	GAGCTC	1 cut(s)	631
SalI	GTCGAC	1 cut(s)	575
SaqAI	TTAA	1 cut(s)	413
SatI	GCNGC	8 cut(s)	39, 152, 195, 207, 210, 297, 485, 851
Sau96I	GGNCC	4 cut(s)	247, 681, 962, 984
ScaI	AGTACT	1 cut(s)	638
ScrFI	CCNGG	2 cut(s)	712, 960
SduI	GDGCHC	2 cut(s)	631, 1054
SfaNI	GCATC	1 cut(s)	904
SfcI	CTRYAG	1 cut(s)	943
Sfr274I	CTCGAG	2 cut(s)	479, 524
SgrAI	CRCCGGYG	1 cut(s)	670
SgrDI	CGTCGACG	1 cut(s)	575
SinI	GGWCC	4 cut(s)	247, 681, 962, 984
SlaI	CTCGAG	2 cut(s)	479, 524
SmiMI	CAYNNNNRTG	2 cut(s)	740, 788
SmlI	CTYRAG	3 cut(s)	314, 479, 524
SmoI	CTYRAG	3 cut(s)	314, 479, 524
Sse9I	AATT	4 cut(s)	404, 410, 493, 505
SspMI	CTAG	5 cut(s)	374, 501, 512, 554, 758
SstI	GAGCTC	1 cut(s)	631
StyD4I	CCNGG	2 cut(s)	710, 958
StyI	CCWWGG	1 cut(s)	243
TaaI	ACNGT	3 cut(s)	641, 723, 1002
TaiI	ACGT	2 cut(s)	255, 933
TasI	AATT	4 cut(s)	404, 410, 493, 505
TatI	WGTACW	1 cut(s)	636
TauI	GCSGC	7 cut(s)	41, 154, 197, 209, 212, 299, 853
TfiI	GAWTC	9 cut(s)	18, 159, 221, 310, 377, 385, 557, 811, 1016
Tru1I	TTAA	1 cut(s)	413
Tru9I	TTAA	1 cut(s)	413
TseFI	GTSAC	1 cut(s)	641
TseI	GCWGC	1 cut(s)	484
Tsp45I	GTSAC	1 cut(s)	641
TspDTI	ATGAA	2 cut(s)	293, 968
TspGWI	ACGGA	4 cut(s)	116, 454, 460, 800
VpaK11BI	GGWCC	4 cut(s)	247, 681, 962, 984
XbaI	TCTAGA	1 cut(s)	757
XcmI	CCANNNNNNNNNTGG	1 cut(s)	914
XhoI	CTCGAG	2 cut(s)	479, 524
XmiI	GTMKAC	1 cut(s)	576
XspI	CTAG	5 cut(s)	374, 501, 512, 554, 758
ZrmI	AGTACT	1 cut(s)	638

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.